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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB730
Library AF
(Link to library)
Clone ID AFB730
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U09897-1
Original site URL
Representative seq. ID AFB730P
(Link to Original site)
Representative DNA sequence 
>AFB730 (AFB730Q) /CSM/AF/AFB7-B/AFB730Q.Seq.d/
ATTTATTTATTTTAATTTAAAATGTCAACTTTATATNAAATTTTCAAAGATACAACTTCA
AGAGATAATAAAAAAAAATTGATAACAATTCCTATTGCCAAAGAGGATAGTCAATCATTT
GGTTCACTCTCTATCACCTATCAAAAGTTTTTAAATGATTCAGTTAGCTTTGCAAATAAA
TTAATTGAAAAAGGTATTAAAACTGGTGATGTAGTTTCATTAGTTATTACAAATGGTTAT
CCAATTTTATCATGTTTCTTGGGATCAACATTTGCAAGATGTATTGCAGCACCATTAAAT
TCAGCATATAAGAGTGAAGAATTTAATTTCTATTATAAAGATATGGGAGCAAATATTGTT
ATTGTTCAAAAAGGGTTAAGTGAAGCATTGAAATCAGCCAAGGAATTAGGTATTAAAGTT
TGGGAAATTGAAAATGTTGAAAATGAAAAGGAAGGTAAACTTTATTATAGAATTACAGAT
CCAAGCAATGGAGATGACTTGATTTTCTCAAGTGAAACAATTTCAGGAGAGGATGAAGGT
GATAAAATTAAAXXXXXXXXXXGTCCAGTAATTGAGTCCTACGGTATGACAGAGGCTTCC
CACCAAATGGCATCGAACCCATTGCCACAAGACGGTCCAAGAAAGCCTGGTAGCGTCGGT
AAGGGATTCAATGTTCAAATTAGCATTGTCAATGACAATGGCGAGCATCAAAAACAAGGT
GATGTAGGTGAAGTTTGTATCAAGGGCAAGAATGTTATGCATGGTTATCATAATAATCCA
CAAGCAAACATTGATAACTTTACAAAGGATGGTTGGTTCCTCACTGGTGATATCGGTTAT
TTAGATCAAGACGGCTACTTGATTCTCAAGGGTAGAAAGAAGGAAATCATCAATAGAGGT
GGTGAGAAAATCTCCCCATTGGAAGTTGATAATGCTCTCTTGGAAAATGATAAAATTCTC
GAAGCTGTTTGCTTTGGTGTACCAGATGAAAAGTATGGCGAAGAGATTTGGGCTGCCGTC
ATCCCAAAAGTACCTCAATCAATTACAGTCGAAGAAATCACCCAATTCCTCCAAAAGAAA
TTAATCTCTTTCAAAGTGCCAAAGAAAATCATTATCACTGATATTTCCCAAAAACTGCTT
CAGGTAAAATTCAAAGAAGATTCATTGCAGATTTCTTTTNAAACAACAAAATCAATAATT
AATTTAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iylf*FKMSTLYXIFKDTTSRDNKKKLITIPIAKEDSQSFGSLSITYQKFLNDSVSFANK
LIEKGIKTGDVVSLVITNGYPILSCFLGSTFARCIAAPLNSAYKSEEFNFYYKDMGANIV
IVQKGLSEALKSAKELGIKVWEIENVENEKEGKLYYRITDPSNGDDLIFSSETISGEDEG
DKIK---

---PVIESYGMTEASHQMASNPLPQDGPRKPGSVGKGFNVQISIVNDNGEHQKQGDVGEV
CIKGKNVMHGYHNNPQANIDNFTKDGWFLTGDIGYLDQDGYLILKGRKKEIINRGGEKIS
PLEVDNALLENDKILEAVCFGVPDEKYGEEIWAAVIPKVPQSITVEEITQFLQKKLISFK
VPKKIIITDISQKLLQVKFKEDSLQISFXTTKSIINLI
Translated Amino Acid sequence (All Frames) 
Frame A:
iylf*FKMSTLYXIFKDTTSRDNKKKLITIPIAKEDSQSFGSLSITYQKFLNDSVSFANK
LIEKGIKTGDVVSLVITNGYPILSCFLGSTFARCIAAPLNSAYKSEEFNFYYKDMGANIV
IVQKGLSEALKSAKELGIKVWEIENVENEKEGKLYYRITDPSNGDDLIFSSETISGEDEG
DKIK---

---vq*lsptv*qrlptkwhrthchktvqeslvasvrdsmfklalsmtmasiknkvm*vk
fvsrarmlcmviiiihkqtlitlqrmvgsslvisvi*iktat*fsrverrkssievvrks
phwklimlswkmikfsklfalvyqmksmakrfglpssqkylnqlqskkspnsskrn*sls
kcqrkslslifpkncfr*nskkihcrflxkqqnq*li*

Frame B:
fiyfnlkcqlyxkfskiqlqeiikkn**qfllpkrivnhlvhslspiksf*miqlalqin
*lkkvlklvm*fh*llqmviqfyhvswdqhlqdvlqhh*iqhirvknlisiikiweqill
lfkkg*vkh*nqprn*vlkfgklkmlkmkrkvnfiielqiqamemt*fsqvkqfqermkv
ikl---

---ssn*vlrydrgfppngiepiatrrskkaw*rr*giqcsn*hcq*qwrasktr*cr*s
lyqgqecyawls**stskh**lykgwlvphw*yrlfrsrrlldsqg*kegnhq*rw*enl
pigs**cslgk**nsrscllwctr*kvwrrdlgcrhpkstsinysrrnhpippkeinlfq
sakenhyh*yfpktasgkiqrrfiadffxnnkinn*fn

Frame C:
lfili*nvnfixnfqrynfkr**kkidnnsycqrg*siiwftlyhlskvfk*fs*lck*i
n*kry*nw*csfisyykwlsnfimflginickmycstikfsi*e*ri*fll*rygskycy
cskrvk*sieisqgiry*slgn*kc*k*kgr*tll*nyrskqwr*ldflk*nnfrrg*r*
*n*---

---PVIESYGMTEASHQMASNPLPQDGPRKPGSVGKGFNVQISIVNDNGEHQKQGDVGEV
CIKGKNVMHGYHNNPQANIDNFTKDGWFLTGDIGYLDQDGYLILKGRKKEIINRGGEKIS
PLEVDNALLENDKILEAVCFGVPDEKYGEEIWAAVIPKVPQSITVEEITQFLQKKLISFK
VPKKIIITDISQKLLQVKFKEDSLQISFXTTKSIINLI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB730 (AFB730Q) /CSM/AF/AFB7-B/AFB730Q.Seq.d/                       2272   0.0
SFG669 (SFG669Q) /CSM/SF/SFG6-C/SFG669Q.Seq.d/                       1229   0.0
CFA349 (CFA349Q) /CSM/CF/CFA3-C/CFA349Q.Seq.d/                       1227   0.0
CFG480 (CFG480Q) /CSM/CF/CFG4-D/CFG480Q.Seq.d/                       1221   0.0
AFG820 (AFG820Q) /CSM/AF/AFG8-A/AFG820Q.Seq.d/                       1221   0.0
VFF556 (VFF556Q) /CSM/VF/VFF5-C/VFF556Q.Seq.d/                       1219   0.0
CFI281 (CFI281Q) /CSM/CF/CFI2-D/CFI281Q.Seq.d/                       1219   0.0
CFH325 (CFH325Q) /CSM/CF/CFH3-B/CFH325Q.Seq.d/                       1219   0.0
SFJ267 (SFJ267Q) /CSM/SF/SFJ2-C/SFJ267Q.Seq.d/                       1217   0.0
SFF865 (SFF865Q) /CSM/SF/SFF8-C/SFF865Q.Seq.d/                       1217   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BQ864280|BQ864280.1 QGC26E11.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC26E11, mRNA sequence.    52  0.002  2
AC022325|AC022325.5 Homo sapiens BAC clone RP11-391J13 from 4, complete sequence.    46  0.043  7
AF045640|AF045640.2 Caenorhabditis elegans cosmid C11D2, complete sequence.    38  0.12  4
AF482758|AF482758.2 Cowpox virus strain Brighton Red, complete genome.    40  0.16  8
AE014850|AE014850.1 Plasmodium falciparum 3D7 chromosome 12 section 7 of 9 of the complete sequence.    42  0.16  6
AC116922|AC116922.1 Dictyostelium discoideum chromosome 2 map 4969700-4990227 strain AX4, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    34  0.20  4
CB855921|CB855921.1 ri45h01.y1 Meloidogyne chitwoodi J2 pDNR LIB Meloidogyne chitwoodi cDNA 5' similar to TR:O16482 O16482 SIMILAR TO CYTOCHROME P450. [1] ;, mRNA sequence.    42  0.26  2
AC138014|AC138014.12 Medicago truncatula clone mth2-17p13, WORKING DRAFT SEQUENCE, 5 ordered pieces.    40  0.27  6
AB015477|AB015477.1 Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone:MOK9.    38  0.27  7
AC104155|AC104155.2 Pan troglodytes clone RP43-129C17, WORKING DRAFT SEQUENCE, 4 ordered pieces.    46  0.31  2
dna update 2003. 7.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF354454_1(AF354454|pid:none) Capsicum annuum putative acyl-CoA ...   224   5e-57
CR382131_531(CR382131|pid:none) Yarrowia lipolytica strain CLIB1...   216   7e-57
EF676481_1(EF676481|pid:none) Picea sitchensis clone WS02739_F21...   217   7e-55
CR380957_291(CR380957|pid:none) Candida glabrata strain CBS138 c...   215   3e-54
EU974597_1(EU974597|pid:none) Zea mays clone 453960 peroxisomal-...   209   3e-54
AL606651_2(AL606651|pid:none) Oryza sativa genomic DNA, chromoso...   209   2e-52
(P38137) RecName: Full=Peroxisomal-coenzyme A synthetase;       ...   202   2e-52
AM270070_4(AM270070|pid:none) Aspergillus niger contig An04c0090...   208   3e-52
AP007157_804(AP007157|pid:none) Aspergillus oryzae RIB40 genomic...   207   6e-52
(O74976) RecName: Full=Putative peroxisomal-coenzyme A synthetas...   207   7e-52
protein update 2009. 3.27
PSORT 

 psg: 0.33  gvh: 0.32  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for AFB730 is nuc
5' end seq. ID AFB730F
5' end seq. 
>AFB730F.Seq
ATTTATTTATTTTAATTTAAAATGTCAACTTTATATNAAATTTTCAAAGATACAACTTCA
AGAGATAATAAAAAAAAATTGATAACAATTCCTATTGCCAAAGAGGATAGTCAATCATTT
GGTTCACTCTCTATCACCTATCAAAAGTTTTTAAATGATTCAGTTAGCTTTGCAAATAAA
TTAATTGAAAAAGGTATTAAAACTGGTGATGTAGTTTCATTAGTTATTACAAATGGTTAT
CCAATTTTATCATGTTTCTTGGGATCAACATTTGCAAGATGTATTGCAGCACCATTAAAT
TCAGCATATAAGAGTGAAGAATTTAATTTCTATTATAAAGATATGGGAGCAAATATTGTT
ATTGTTCAAAAAGGGTTAAGTGAAGCATTGAAATCAGCCAAGGAATTAGGTATTAAAGTT
TGGGAAATTGAAAATGTTGAAAATGAAAAGGAAGGTAAACTTTATTATAGAATTACAGAT
CCAAGCAATGGAGATGACTTGATTTTCTCAAGTGAAACAATTTCAGGAGAGGATGAAGGT
GATAAAATTAAA----------
Length of 5' end seq. 552
3' end seq. ID AFB730Z
3' end seq. 
>AFB730Z.Seq
----------GTCCAGTAATTGAGTCCTACGGTATGACAGAGGCTTCCCACCAAATGGCA
TCGAACCCATTGCCACAAGACGGTCCAAGAAAGCCTGGTAGCGTCGGTAAGGGATTCAAT
GTTCAAATTAGCATTGTCAATGACAATGGCGAGCATCAAAAACAAGGTGATGTAGGTGAA
GTTTGTATCAAGGGCAAGAATGTTATGCATGGTTATCATAATAATCCACAAGCAAACATT
GATAACTTTACAAAGGATGGTTGGTTCCTCACTGGTGATATCGGTTATTTAGATCAAGAC
GGCTACTTGATTCTCAAGGGTAGAAAGAAGGAAATCATCAATAGAGGTGGTGAGAAAATC
TCCCCATTGGAAGTTGATAATGCTCTCTTGGAAAATGATAAAATTCTCGAAGCTGTTTGC
TTTGGTGTACCAGATGAAAAGTATGGCGAAGAGATTTGGGCTGCCGTCATCCCAAAAGTA
CCTCAATCAATTACAGTCGAAGAAATCACCCAATTCCTCCAAAAGAAATTAATCTCTTTC
AAAGTGCCAAAGAAAATCATTATCACTGATATTTCCCAAAAACTGCTTCAGGTAAAATTC
AAAGAAGATTCATTGCAGATTTCTTTTNAAACAACAAAATCAATAATTAATTTAATA
Length of 3' end seq. 647
Connected seq. ID AFB730P
Connected seq. 
>AFB730P.Seq
ATTTATTTATTTTAATTTAAAATGTCAACTTTATATNAAATTTTCAAAGATACAACTTCA
AGAGATAATAAAAAAAAATTGATAACAATTCCTATTGCCAAAGAGGATAGTCAATCATTT
GGTTCACTCTCTATCACCTATCAAAAGTTTTTAAATGATTCAGTTAGCTTTGCAAATAAA
TTAATTGAAAAAGGTATTAAAACTGGTGATGTAGTTTCATTAGTTATTACAAATGGTTAT
CCAATTTTATCATGTTTCTTGGGATCAACATTTGCAAGATGTATTGCAGCACCATTAAAT
TCAGCATATAAGAGTGAAGAATTTAATTTCTATTATAAAGATATGGGAGCAAATATTGTT
ATTGTTCAAAAAGGGTTAAGTGAAGCATTGAAATCAGCCAAGGAATTAGGTATTAAAGTT
TGGGAAATTGAAAATGTTGAAAATGAAAAGGAAGGTAAACTTTATTATAGAATTACAGAT
CCAAGCAATGGAGATGACTTGATTTTCTCAAGTGAAACAATTTCAGGAGAGGATGAAGGT
GATAAAATTAAA----------GTCCAGTAATTGAGTCCTACGGTATGACAGAGGCTTCC
CACCAAATGGCATCGAACCCATTGCCACAAGACGGTCCAAGAAAGCCTGGTAGCGTCGGT
AAGGGATTCAATGTTCAAATTAGCATTGTCAATGACAATGGCGAGCATCAAAAACAAGGT
GATGTAGGTGAAGTTTGTATCAAGGGCAAGAATGTTATGCATGGTTATCATAATAATCCA
CAAGCAAACATTGATAACTTTACAAAGGATGGTTGGTTCCTCACTGGTGATATCGGTTAT
TTAGATCAAGACGGCTACTTGATTCTCAAGGGTAGAAAGAAGGAAATCATCAATAGAGGT
GGTGAGAAAATCTCCCCATTGGAAGTTGATAATGCTCTCTTGGAAAATGATAAAATTCTC
GAAGCTGTTTGCTTTGGTGTACCAGATGAAAAGTATGGCGAAGAGATTTGGGCTGCCGTC
ATCCCAAAAGTACCTCAATCAATTACAGTCGAAGAAATCACCCAATTCCTCCAAAAGAAA
TTAATCTCTTTCAAAGTGCCAAAGAAAATCATTATCACTGATATTTCCCAAAAACTGCTT
CAGGTAAAATTCAAAGAAGATTCATTGCAGATTTCTTTTNAAACAACAAAATCAATAATT
AATTTAATA
Length of connected seq. 1199
Full length Seq ID -
Full length Seq. -
Length of full length seq. -