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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHN532
Library VH
(Link to library)
Clone ID VHN532
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15717-1
Original site URL
Representative seq. ID VHN532P
(Link to Original site)
Representative DNA sequence 
>VHN532 (VHN532Q) /CSM/VH/VHN5-B/VHN532Q.Seq.d/
TATTTTTTTTTATATTTATATAATTAAAAAAAAAATAATTNTAAAATGAACTCACTCGTT
AAGGGTATTTCCAAAGTTAGATCAACTNNATCTTTTTCAACAGTATCAATGAGTCCTCTT
GAACCAAACAANAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTCANACTC
CATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATGGTCATTTANAANATCCA
TCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCANATCGTGTTGCTATG
CAANATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCANAAACT
GCAGTACCAACAACAATTCATTGNGATCATTTAATTGAAGCATATAAAGGNGGTGAAAAA
NATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACCAGTGCT
AAAAAATTTGGTATGGGTTTCTGGAAGCXXXXXXXXXXAAGCAGCAGATTACAATTACTC
CAACCATTTGCCCCATGGGATAAGAAANATTTAGTCGATATGCAAGTTTTCATCAAGGTT
CAAGGTAAATGNACCNCCGATCACATNTNTATGGNTGGTCCATGGTTGAAANACANAGGT
CATTTGGATAACATTTCCAACAATATGTTAATTGGNGCTATCAACTNTGAAAACGGTAAA
GCCAACGCTGTTTTCAATCAATTCACTGGTGAAATCGGTCCAGTCCCAACCGTCGNTNGT
GANTACAANAAACGTGGTGTCAATTGGATNGTCGTTGGTGATGAAAACTATGGTGAAGGT
TCATNTNGTGAACATGCTGCCCTCGAACCACGTCATNTCGGTGGTAAAGCTATCCTCGTT
AAATCATTTGNTNGNATCCNCGAAACCAATNTCAANAAACAAGGTATNTTACCATTAACC
TTTGNTAACCCATNTGATTACGATAAAATCTNTGGTGACGATNGTATTTCAATCATTGGT
NTCAAANATCTTGCCCCAGGTAAACAACTCACTCTCATTGTTAAATNTGNTAAACAAGGT
TNTGAATTTGAAATTAAAGCTAATCAC
sequence update 2002.10.25
Translated Amino Acid sequence 
yfflylyn*KKNNXKMNSLVKGISKVRSTXSFSTVSMSPLEPNXKLNYEGIDAKLKQFXL
HHNKPLTLAEKIIYGHLXXPSTKVERGITYLKLHPXRVAMQXATAQMAVLQFMSAGLPXT
AVPTTIHXDHLIEAYKGGEKXLEVAKDINKEVYDFLSTSAKKFGMGFWK---

---SSRLQLLQPFAPWDKKXLVDMQVFIKVQGKXTXDHXXMXGPWLKXXGHLDNISNNML
IGAINXENGKANAVFNQFTGEIGPVPTVXXXYXKRGVNWXVVGDENYGEGSXXEHAALEP
RHXGGKAILVKSFXXIXETNXXKQGXLPLTFXNPXDYDKIXGDDXISIIGXKXLAPGKQL
TLIVKXXKQGXEFEIKANH
Translated Amino Acid sequence (All Frames) 
Frame A:
yfflylyn*KKNNXKMNSLVKGISKVRSTXSFSTVSMSPLEPNXKLNYEGIDAKLKQFXL
HHNKPLTLAEKIIYGHLXXPSTKVERGITYLKLHPXRVAMQXATAQMAVLQFMSAGLPXT
AVPTTIHXDHLIEAYKGGEKXLEVAKDINKEVYDFLSTSAKKFGMGFWK---

---kqqititpticpmg*exfsryasfhqgsr*mxxrshxygwsmvexxrsfg*hfqqyv
nwxyql*kr*sqrcfqsihw*nrsspnrrx*xqxtwcqldxrw**klw*rfxx*tccprt
tsxrw*sypr*iixxxprnqxqxtryxtinlx*px*lr*nlw*rxyfnhwxqxscpr*tt
hshc*xx*trx*i*n*s*s

Frame B:
iffyiyiikkkiixk*thslrvfpkldqlxlfqqyq*vllnqtxnsttkvlmln*nnsxs
ittnhspllkrsfmvixxihqlklkevslilnyixivllcxmpphkwlyynlcqlvyxkl
qyqqqfixii*lkhikxvkxi*klqkisikkftisfqpvlknlvwvsgs---

---SSRLQLLQPFAPWDKKXLVDMQVFIKVQGKXTXDHXXMXGPWLKXXGHLDNISNNML
IGAINXENGKANAVFNQFTGEIGPVPTVXXXYXKRGVNWXVVGDENYGEGSXXEHAALEP
RHXGGKAILVKSFXXIXETNXXKQGXLPLTFXNPXDYDKIXGDDXISIIGXKXLAPGKQL
TLIVKXXKQGXEFEIKANH

Frame C:
fffifi*lkkk*x*neltr*gyfqs*inxiffnsiness*tkqxtqlrry*c*iktixtp
sqqtthpc*kdhlwsfxxsin*s*kryhls*itsxsccyaxchrtngcitiyvswftxnc
stnnnslxsfn*si*rx*kxfrsckryq*rslrfpfnqc*kiwygfle---

---aadynysnhlphgirxi*sickfssrfkvnxppitxxwxvhg*xtxviwitfptic*
lxlstxktvkptlfsinslvksvqsqpsxvxtxnvvsigxslvmktmvkvhxvnmlpsnh
vxsvvklsslnhlxxsxkpxsxnkvxyh*plxthxitiksxvtxvfqslvsxilpqvnns
lsllnxxnkvxnlklkli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN532 (VHN532Q) /CSM/VH/VHN5-B/VHN532Q.Seq.d/                       1796   0.0
VHO133 (VHO133Q) /CSM/VH/VHO1-B/VHO133Q.Seq.d/                        950   0.0
VHJ814 (VHJ814Q) /CSM/VH/VHJ8-A/VHJ814Q.Seq.d/                        950   0.0
VHJ504 (VHJ504Q) /CSM/VH/VHJ5-A/VHJ504Q.Seq.d/                        950   0.0
VHD386 (VHD386Q) /CSM/VH/VHD3-D/VHD386Q.Seq.d/                        950   0.0
VHC367 (VHC367Q) /CSM/VH/VHC3-C/VHC367Q.Seq.d/                        942   0.0
CHQ607 (CHQ607Q) /CSM/CH/CHQ6-A/CHQ607Q.Seq.d/                        938   0.0
AHJ143 (AHJ143Q) /CSM/AH/AHJ1-B/AHJ143Q.Seq.d/                        938   0.0
VHF201 (VHF201Q) /CSM/VH/VHF2-A/VHF201Q.Seq.d/                        932   0.0
VHJ867 (VHJ867Q) /CSM/VH/VHJ8-C/VHJ867Q.Seq.d/                        926   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445101) Dictyostelium discoideum cDNA clone:ddv58o07, 3' ...   965   0.0    1
(BJ445359) Dictyostelium discoideum cDNA clone:ddv59a09, 3' ...   950   0.0    1
(BJ442525) Dictyostelium discoideum cDNA clone:ddv50l04, 3' ...   950   0.0    1
(BJ442499) Dictyostelium discoideum cDNA clone:ddv50g01, 3' ...   950   0.0    1
(BJ438430) Dictyostelium discoideum cDNA clone:ddv37l21, 3' ...   950   0.0    1
(BJ437847) Dictyostelium discoideum cDNA clone:ddv35f17, 3' ...   942   0.0    1
(BJ385501) Dictyostelium discoideum cDNA clone:ddc55m02, 3' ...   938   0.0    1
(BJ353194) Dictyostelium discoideum cDNA clone:dda49e11, 3' ...   938   0.0    1
(BJ439599) Dictyostelium discoideum cDNA clone:ddv41a02, 3' ...   932   0.0    1
(BJ442653) Dictyostelium discoideum cDNA clone:ddv50f18, 3' ...   904   0.0    2
dna update 2008.12. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Z49931_1(Z49931|pid:none) B.taurus mRNA for mitochondrial aconit...   221   4e-56
DQ468384_1(DQ468384|pid:none) Bos taurus BTA05 5597 genomic sequ...   221   4e-56
BC102642_1(BC102642|pid:none) Bos taurus aconitase 2, mitochondr...   221   4e-56
(P20004) RecName: Full=Aconitate hydratase, mitochondrial;      ...   221   4e-56
(Q99KI0) RecName: Full=Aconitate hydratase, mitochondrial;      ...   219   1e-55
AK144207_1(AK144207|pid:none) Mus musculus kidney CCL-142 RAG cD...   219   1e-55
AK297213_1(AK297213|pid:none) Homo sapiens cDNA FLJ52327 complet...   218   3e-55
AK314845_1(AK314845|pid:none) Homo sapiens cDNA, FLJ95737, highl...   218   3e-55
CR536568_1(CR536568|pid:none) Homo sapiens full open reading fra...   218   3e-55
AK296260_1(AK296260|pid:none) Homo sapiens cDNA FLJ54329 complet...   218   3e-55
protein update 2009. 5.16
PSORT 

 psg: 0.75  gvh: 0.42  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 24.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHN532 is mit
5' end seq. ID VHN532F
5' end seq. 
>VHN532F.Seq
TATTTTTTTTTATATTTATATAATTAAAAAAAAAATAATTNTAAAATGAACTCACTCGTT
AAGGGTATTTCCAAAGTTAGATCAACTNNATCTTTTTCAACAGTATCAATGAGTCCTCTT
GAACCAAACAANAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTCANACTC
CATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATGGTCATTTANAANATCCA
TCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCANATCGTGTTGCTATG
CAANATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCANAAACT
GCAGTACCAACAACAATTCATTGNGATCATTTAATTGAAGCATATAAAGGNGGTGAAAAA
NATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACCAGTGCT
AAAAAATTTGGTATGGGTTTCTGGAAGCNNNNNNNNNN
Length of 5' end seq. 518
3' end seq. ID VHN532Z
3' end seq. 
>VHN532Z.Seq
NNNNNNNNNNAAGCAGCAGATTACAATTACTCCAACCATTTGCCCCATGGGATAAGAAAN
ATTTAGTCGATATGCAAGTTTTCATCAAGGTTCAAGGTAAATGNACCNCCGATCACATNT
NTATGGNTGGTCCATGGTTGAAANACANAGGTCATTTGGATAACATTTCCAACAATATGT
TAATTGGNGCTATCAACTNTGAAAACGGTAAAGCCAACGCTGTTTTCAATCAATTCACTG
GTGAAATCGGTCCAGTCCCAACCGTCGNTNGTGANTACAANAAACGTGGTGTCAATTGGA
TNGTCGTTGGTGATGAAAACTATGGTGAAGGTTCATNTNGTGAACATGCTGCCCTCGAAC
CACGTCATNTCGGTGGTAAAGCTATCCTCGTTAAATCATTTGNTNGNATCCNCGAAACCA
ATNTCAANAAACAAGGTATNTTACCATTAACCTTTGNTAACCCATNTGATTACGATAAAA
TCTNTGGTGACGATNGTATTTCAATCATTGGTNTCAAANATCTTGCCCCAGGTAAACAAC
TCACTCTCATTGTTAAATNTGNTAAACAAGGTTNTGAATTTGAAATTAAAGCTAATCAC
Length of 3' end seq. 599
Connected seq. ID VHN532P
Connected seq. 
>VHN532P.Seq
TATTTTTTTTTATATTTATATAATTAAAAAAAAAATAATTNTAAAATGAACTCACTCGTT
AAGGGTATTTCCAAAGTTAGATCAACTNNATCTTTTTCAACAGTATCAATGAGTCCTCTT
GAACCAAACAANAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTCANACTC
CATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATGGTCATTTANAANATCCA
TCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCANATCGTGTTGCTATG
CAANATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCANAAACT
GCAGTACCAACAACAATTCATTGNGATCATTTAATTGAAGCATATAAAGGNGGTGAAAAA
NATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACCAGTGCT
AAAAAATTTGGTATGGGTTTCTGGAAGC----------AAGCAGCAGATTACAATTACTC
CAACCATTTGCCCCATGGGATAAGAAANATTTAGTCGATATGCAAGTTTTCATCAAGGTT
CAAGGTAAATGNACCNCCGATCACATNTNTATGGNTGGTCCATGGTTGAAANACANAGGT
CATTTGGATAACATTTCCAACAATATGTTAATTGGNGCTATCAACTNTGAAAACGGTAAA
GCCAACGCTGTTTTCAATCAATTCACTGGTGAAATCGGTCCAGTCCCAACCGTCGNTNGT
GANTACAANAAACGTGGTGTCAATTGGATNGTCGTTGGTGATGAAAACTATGGTGAAGGT
TCATNTNGTGAACATGCTGCCCTCGAACCACGTCATNTCGGTGGTAAAGCTATCCTCGTT
AAATCATTTGNTNGNATCCNCGAAACCAATNTCAANAAACAAGGTATNTTACCATTAACC
TTTGNTAACCCATNTGATTACGATAAAATCTNTGGTGACGATNGTATTTCAATCATTGGT
NTCAAANATCTTGCCCCAGGTAAACAACTCACTCTCATTGTTAAATNTGNTAAACAAGGT
TNTGAATTTGAAATTAAAGCTAATCAC
Length of connected seq. 1097
Full length Seq ID -
Full length Seq. -
Length of full length seq. -