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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHN529
Library VH
(Link to library)
Clone ID VHN529
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15137-1
Original site URL
Representative seq. ID VHN529P
(Link to Original site)
Representative DNA sequence 
>VHN529 (VHN529Q) /CSM/VH/VHN5-B/VHN529Q.Seq.d/
TACAATGACTTGGCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCATCAGC
TGCTCTTTTAGGTGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATGTCGC
TGAAGGTAAGGCAATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTGGCAT
CCACGTTGCTGGTAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTACGGTAA
ATTAGGTGCTGGTGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTGTCTA
TGATGACAAATTACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATTATTT
AATTGACAACAACTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAATATA
TAAAATAAAAAAAAAAXXXXXXXXXXATTTTCATACACTCAAAACTCTTAATAAATATAT
AATATACAATGACTTGGCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCAT
CAGCTGCTCTTTTAGGTGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATG
TCGCTGAAGGTAAGGCAATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTG
GCATCCACGTTGCTGGTAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTACG
GTAAATTAGGTGCTGGTGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTG
TCTATGATGACAAATTACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATT
ATTTAATTGACAACAACTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAA
TAT
sequence update 2002.10.25
Translated Amino Acid sequence 
tmtwqayvdnnllgagfasaallgaadgsvwahsagfnvaegkaitalfqkdgaafatgi
hvagkkymaiksdtrsaygklgaggvvcvktltciivavyddklqpgaaaniaekladyl
idnnc*isilfknk*RKIIYKIKKK---

---FHTLKTLNKYIIYNDLASIRR*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gn
nrtfskgwscirnwhprcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qit
trccc*hc*kisrlfn*qqlln*ysfqk*it*nn
Translated Amino Acid sequence (All Frames) 
Frame A:
yndlasirr*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gnnrtfskgwscirnwh
prcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qittrccc*hc*kisrlf
n*qqlln*ysfqk*it*nni*nkkk---

---ifihskllini*ytmtwqayvdnnllgagfasaallgaadgsvwahsagfnvaegka
italfqkdgaafatgihvagkkymaiksdtrsaygklgaggvvcvktltciivavyddkl
qpgaaaniaekladylidnnc*isilfknk*rkii

Frame B:
tmtwqayvdnnllgagfasaallgaadgsvwahsagfnvaegkaitalfqkdgaafatgi
hvagkkymaiksdtrsaygklgaggvvcvktltciivavyddklqpgaaaniaekladyl
idnnc*isilfknk*RKIIYKIKKK---

---fsytqns**iyniq*lgkhtsittylvlvshqllf*vlltvvfghtqldsmslkvrq
*phffkrmelhsqlastllvrniwpskvtpdlltvn*vlvvlfalkp*lvssllsmmtny
nqvllltllkn*pii*ltttvklvffskinnvk*y

Frame C:
q*lgkhtsittylvlvshqllf*vlltvvfghtqldsmslkvrq*phffkrmelhsqlas
tllvrniwpskvtpdlltvn*vlvvlfalkp*lvssllsmmtnynqvllltllkn*pii*
ltttvklvffskinnvk*yik*kk---

---FHTLKTLNKYIIYNDLASIRR*qltwcwfriscsfrcc*r*clgtlswiqcr*r*gn
nrtfskgwscirnwhprcw*eiyghqk*hqiclr*ircwwcclr*nlnlyhrccl**qit
trccc*hc*kisrlfn*qqlln*ysfqk*it*nn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN529 (VHN529Q) /CSM/VH/VHN5-B/VHN529Q.Seq.d/                        906   0.0
VHH513 (VHH513Q) /CSM/VH/VHH5-A/VHH513Q.Seq.d/                        906   0.0
VFI876 (VFI876Q) /CSM/VF/VFI8-D/VFI876Q.Seq.d/                        906   0.0
VFK707 (VFK707Q) /CSM/VF/VFK7-A/VFK707Q.Seq.d/                        894   0.0
VFE616 (VFE616Q) /CSM/VF/VFE6-A/VFE616Q.Seq.d/                        894   0.0
VSH127 (VSH127Q) /CSM/VS/VSH1-B/VSH127Q.Seq.d/                        876   0.0
SSJ209 (SSJ209Q) /CSM/SS/SSJ2-A/SSJ209Q.Seq.d/                        876   0.0
SSL648 (SSL648Q) /CSM/SS/SSL6-B/SSL648Q.Seq.d/                        866   0.0
SSH172 (SSH172Q) /CSM/SS/SSH1-C/SSH172Q.Seq.d/                        866   0.0
FC-AX21 (FC-AX21Q) /CSM/FC/FC-AX/FC-AX21Q.Seq.d/                      866   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445067) Dictyostelium discoideum cDNA clone:ddv58i07, 3' ...   906   0.0    1
(BJ441237) Dictyostelium discoideum cDNA clone:ddv46i03, 3' ...   906   0.0    1
(BJ422498) Dictyostelium discoideum cDNA clone:ddv46i03, 5' ...   906   0.0    1
(BJ414340) Dictyostelium discoideum cDNA clone:ddv18h20, 5' ...   906   0.0    1
(BJ433534) Dictyostelium discoideum cDNA clone:ddv22n01, 3' ...   894   0.0    1
(BJ427954) Dictyostelium discoideum cDNA clone:ddv10o04, 3' ...   894   0.0    1
(BJ415318) Dictyostelium discoideum cDNA clone:ddv22n01, 5' ...   894   0.0    1
(BJ409836) Dictyostelium discoideum cDNA clone:ddv10o04, 5' ...   894   0.0    1
(AU266945) Dictyostelium discoideum vegetative cDNA clone:VS...   876   0.0    1
(AU266944) Dictyostelium discoideum vegetative cDNA clone:VS...   876   0.0    1
dna update 2008.12. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P26200) RecName: Full=Profilin-2; AltName: Full=Profilin II;  &...   213   9e-54
(Q95VF7) RecName: Full=Profilin-1B; AltName: Full=Profilin IB; A...   105   3e-21
(P68696) RecName: Full=Profilin-1A; AltName: Full=Profilin IA; A...   103   6e-21
C48405(C48405)profilin-IB - Acanthamoeba sp. (tentative sequence)     103   1e-20
NRL(1ACF) Profilin i - Acanthamoeba castellanii                       102   2e-20
NRL(2PRF) Profilin ia (nmr, 19 structures) - Acanthamoeba sp.         102   2e-20
(P39825) RecName: Full=Profilin;  &A53952(A53952;T38785) &CU3296...    97   7e-19
S13199(S13199)profilin - slime mold (Physarum polycephalum)            97   7e-19
(Q8T8M2) RecName: Full=Profilin-3; AltName: Full=Profilin III;  ...    96   2e-18
CR382128_274(CR382128|pid:none) Yarrowia lipolytica strain CLIB1...    89   2e-16
protein update 2009. 5.16
PSORT 

 psg: 0.70  gvh: 0.10  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.00
 nuc: 0.24  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 12.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: plasma membrane

>> prediction for VHN529 is nuc
5' end seq. ID VHN529F
5' end seq. 
>VHN529F.Seq
TACAATGACTTGGCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCATCAGC
TGCTCTTTTAGGTGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATGTCGC
TGAAGGTAAGGCAATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTGGCAT
CCACGTTGCTGGTAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTACGGTAA
ATTAGGTGCTGGTGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTGTCTA
TGATGACAAATTACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATTATTT
AATTGACAACAACTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAATATA
TAAAATAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 446
3' end seq. ID VHN529Z
3' end seq. 
>VHN529Z.Seq
NNNNNNNNNNATTTTCATACACTCAAAACTCTTAATAAATATATAATATACAATGACTTG
GCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCATCAGCTGCTCTTTTAGG
TGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATGTCGCTGAAGGTAAGGC
AATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTGGCATCCACGTTGCTGG
TAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTACGGTAAATTAGGTGCTGG
TGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTGTCTATGATGACAAATT
ACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATTATTTAATTGACAACAA
CTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAATAT
Length of 3' end seq. 467
Connected seq. ID VHN529P
Connected seq. 
>VHN529P.Seq
TACAATGACTTGGCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCATCAGC
TGCTCTTTTAGGTGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATGTCGC
TGAAGGTAAGGCAATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTGGCAT
CCACGTTGCTGGTAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTACGGTAA
ATTAGGTGCTGGTGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTGTCTA
TGATGACAAATTACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATTATTT
AATTGACAACAACTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAATATA
TAAAATAAAAAAAAAA----------ATTTTCATACACTCAAAACTCTTAATAAATATAT
AATATACAATGACTTGGCAAGCATACGTCGATAACAACTTACTTGGTGCTGGTTTCGCAT
CAGCTGCTCTTTTAGGTGCTGCTGACGGTAGTGTTTGGGCACACTCAGCTGGATTCAATG
TCGCTGAAGGTAAGGCAATAACCGCACTTTTTCAAAAGGATGGAGCTGCATTCGCAACTG
GCATCCACGTTGCTGGTAAGAAATATATGGCCATCAAAAGTGACACCAGATCTGCTTACG
GTAAATTAGGTGCTGGTGGTGTTGTTTGCGTTAAAACCTTAACTTGTATCATCGTTGCTG
TCTATGATGACAAATTACAACCAGGTGCTGCTGCTAACATTGCTGAAAAATTAGCCGATT
ATTTAATTGACAACAACTGTTAAATTAGTATTCTTTTCAAAAATAAATAACGTAAAATAA
TAT
Length of connected seq. 893
Full length Seq ID -
Full length Seq. -
Length of full length seq. -