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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHN330
Library VH
(Link to library)
Clone ID VHN330
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15680-1
Original site URL
Representative seq. ID VHN330P
(Link to Original site)
Representative DNA sequence 
>VHN330 (VHN330Q) /CSM/VH/VHN3-B/VHN330Q.Seq.d/
ACAAAATGTGTAAATATAATGATCTAAATATTTAGTAGAAAAGGTAGATAGATTGGTATT
ACCAGAAAATGTTGTACCAATTAAATATGATTTACATTTAAAACCAAATTTAAAAGAATT
TACTTTTAAAGGTGAAGAGACCATCACAGTTCAAGTAAAACAACCAACAAAAACCATAAC
TATACATTCAATTGAAATTGAAATTCAATCAGCTTCAATAAAATCATCATCATCATCTCA
ATCATCAAAATCAATTACATTTTATGAACCAGAAGAAGTTGTCATCTTTGAATTTGAAAA
TGAATTATCAGTTGGTGAATATTGTTTATCATTGGTATTTACTGGTTTATTAAATGACAA
ATTAAAAGGTTTCTATCGTTCAAAGTATACTGTTAAAGGTGAAGATCGTTATTTAGCAAC
AACTCAATTCGAAGCCACCGATGCTCGTCGTTCTTTTCCATGTTTTGATGAACCAGCTCA
TAAAGCAGTTTTCAATATCACTTTAACTGTCTCTGAATGTCATACCGCTATTTCAAATAT
GGAAGAAAAATCAATTACTCCAAATAATGATGGTACTAAAACTTATATATTCGAACAXXX
XXXXXXXTAAGAAATAAAGTAAACTCTTATTTGGGTATTTTGGGTGACAAAGAGATTGTG
GCCGAGGCTAGAAAGAGATTTGAGCAATTCAAAGTCGACCAATCTTCTTTACCTTCCGAT
ATCCGTTCAAGTGTTTTAGTCACTGTTGTAAAGAATGGTTCCGAAGCTGAACAACAAGAA
ATTATCAATAGATACCTCGCTTCCAATGATATTGCAGAGAAATCAAGTTTATTGTCAGTT
GTTTGCAAATCACCATCCAGTGCATTGGTATTGAAGGCTTTGGAATTCTCTGTCTCAAAA
GATGTTAGAACTTGTGAATCCTATATGCTTTGGAGAGTCGGAAATGAATTTAAACCAGTA
GTTTGGAAATATTTCACTGAAAACTTTAAATCAATCAATGAAACCTTTAATCAAAATGTT
TTATTCGCTTATATGATCTCTTTTGCTTTGTCCTCAAAAATGACTGATCAACAATTACAA
CAAGTTGAAGATTTCTTCAAACAAAATCCAGTTGCAATTGCTGATCGTTCAATTAAACAA
GATTTAGAACAAATTAGAAATAATACAAAATGGTTTATTCATTTAATAAAGATTTATTAA
ATGGATTAAAAAATAAATATCTATTAATAAAGATTTATAAATTGGATTTTANAAAAATAA
AATTATTAATTTTATATTTTTTA
sequence update 2002.10.25
Translated Amino Acid sequence 
qnv*i**SKYLVEKVDRLVLPENVVPIKYDLHLKPNLKEFTFKGEETITVQVKQPTKTIT
IHSIEIEIQSASIKSSSSSQSSKSITFYEPEEVVIFEFENELSVGEYCLSLVFTGLLNDK
LKGFYRSKYTVKGEDRYLATTQFEATDARRSFPCFDEPAHKAVFNITLTVSECHTAISNM
EEKSITPNNDGTKTYIFE---

---RNKVNSYLGILGDKEIVAEARKRFEQFKVDQSSLPSDIRSSVLVTVVKNGSEAEQQE
IINRYLASNDIAEKSSLLSVVCKSPSSALVLKALEFSVSKDVRTCESYMLWRVGNEFKPV
VWKYFTENFKSINETFNQNVLFAYMISFALSSKMTDQQLQQVEDFFKQNPVAIADRSIKQ
DLEQIRNNTKWFIHLIKIY*md*kiniy**rfinwilxk*ny*fyif
Translated Amino Acid sequence (All Frames) 
Frame A:
tkcvnimi*ifsrkgr*igitrkcctn*i*ftfktkfkriyf*r*rdhhssskttnknhn
ytfn*n*nsisfnkiiiiisiikinyil*trrschl*i*k*iisw*ilfiigiywfik*q
ikrflsfkvyc*r*rslfsnnsirshrcssffsmf**tss*ssfqyhfncl*msyryfky
grkinysk**wy*nlyirt---

---*eik*tliwvfwvtkrlwprlerdlsnskstnllylpisvqvf*sll*rmvpklnnk
klsidtslpmilqrnqvycqlfanhhpvhwy*rlwnslsqkmlelvnpicfgesemnlnq
*fgnislktlnqsmkplikmfysli*slllcpqk*linnynklkissnkiqlqllivqln
ki*nkleiiqnglfi**rfikwikk*isinkdl*igfxknkiinfiff

Frame B:
qnv*i**SKYLVEKVDRLVLPENVVPIKYDLHLKPNLKEFTFKGEETITVQVKQPTKTIT
IHSIEIEIQSASIKSSSSSQSSKSITFYEPEEVVIFEFENELSVGEYCLSLVFTGLLNDK
LKGFYRSKYTVKGEDRYLATTQFEATDARRSFPCFDEPAHKAVFNITLTVSECHTAISNM
EEKSITPNNDGTKTYIFE---

---kk*skllfgyfg*qrdcgrg*kei*aiqsrpifftfrypfkcfshcckewfrs*ttr
nyq*iprfq*ycreikfivsclqitiqcigiegfgilclkrc*nl*ilyalesrk*i*ts
sleifh*kl*inq*nl*skcfirlydlfcfvlknd*stitts*rflqtksscnc*sfn*t
rfrtn*k*ykmvysfnkdllnglknkyllikiykldfxkikllilyfl

Frame C:
kmckyndlni**kr*idwyyqkmlyqlnmiyi*nqi*knlllkvkrpsqfk*nnqqkp*l
yiqlklkfnqlq*nhhhhlnhqnqlhfmnqkklsslnlkmnyqlvnivyhwyllvy*mtn
*kvsivqsillkvkivi*qqlnskppmlvvlfhvlmnqlikqfsisl*lslnviplfqiw
kknqllqimmvlkliysn---

---RNKVNSYLGILGDKEIVAEARKRFEQFKVDQSSLPSDIRSSVLVTVVKNGSEAEQQE
IINRYLASNDIAEKSSLLSVVCKSPSSALVLKALEFSVSKDVRTCESYMLWRVGNEFKPV
VWKYFTENFKSINETFNQNVLFAYMISFALSSKMTDQQLQQVEDFFKQNPVAIADRSIKQ
DLEQIRNNTKWFIHLIKIY*md*kiniy**rfinwilxk*ny*fyif
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN330 (VHN330Q) /CSM/VH/VHN3-B/VHN330Q.Seq.d/                       1778   0.0
AHJ768 (AHJ768Q) /CSM/AH/AHJ7-C/AHJ768Q.Seq.d/                       1136   0.0
VHP819 (VHP819Q) /CSM/VH/VHP8-A/VHP819Q.Seq.d/                       1130   0.0
VHP741 (VHP741Q) /CSM/VH/VHP7-B/VHP741Q.Seq.d/                       1130   0.0
VHO863 (VHO863Q) /CSM/VH/VHO8-C/VHO863Q.Seq.d/                       1130   0.0
VHO805 (VHO805Q) /CSM/VH/VHO8-A/VHO805Q.Seq.d/                       1130   0.0
VHN658 (VHN658Q) /CSM/VH/VHN6-C/VHN658Q.Seq.d/                       1130   0.0
VHN267 (VHN267Q) /CSM/VH/VHN2-C/VHN267Q.Seq.d/                       1130   0.0
VHJ865 (VHJ865Q) /CSM/VH/VHJ8-C/VHJ865Q.Seq.d/                       1130   0.0
VHJ837 (VHJ837Q) /CSM/VH/VHJ8-B/VHJ837Q.Seq.d/                       1130   0.0
own update 2005. 1. 1
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446284) Dictyostelium discoideum cDNA clone:ddv62b11, 3' ...  1122   0.0    1
(BJ446229) Dictyostelium discoideum cDNA clone:ddv62f06, 3' ...  1122   0.0    1
(BJ445800) Dictyostelium discoideum cDNA clone:ddv60n16, 3' ...  1122   0.0    1
(BJ445630) Dictyostelium discoideum cDNA clone:ddv60j02, 3' ...  1122   0.0    1
(BJ445125) Dictyostelium discoideum cDNA clone:ddv58c16, 3' ...  1122   0.0    1
(BJ444821) Dictyostelium discoideum cDNA clone:ddv57e18, 3' ...  1122   0.0    1
(BJ444767) Dictyostelium discoideum cDNA clone:ddv57l07, 3' ...  1122   0.0    1
(BJ442692) Dictyostelium discoideum cDNA clone:ddv50n17, 3' ...  1122   0.0    1
(BJ442634) Dictyostelium discoideum cDNA clone:ddv50b18, 3' ...  1122   0.0    1
(BJ442595) Dictyostelium discoideum cDNA clone:ddv50j10, 3' ...  1122   0.0    1
dna update 2008.12. 5
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY631393_1(AY631393|pid:none) Dictyostelium discoideum puromycin...   364   4e-99
AF350242_1(AF350242|pid:none) Pichia angusta aminopeptidase gene...   136   2e-30
AE014296_282(AE014296|pid:none) Drosophila melanogaster chromoso...   135   2e-30
AE014296_281(AE014296|pid:none) Drosophila melanogaster chromoso...   135   2e-30
AF327435_1(AF327435|pid:none) Drosophila melanogaster puromycin-...   135   3e-30
(P55786) RecName: Full=Puromycin-sensitive aminopeptidase;      ...   128   4e-28
BC098978_1(BC098978|pid:none) Xenopus laevis hypothetical protei...   127   1e-27
A65888_1(A65888|pid:none) Sequence 1 from Patent WO9738114.  &Y0...   126   1e-27
AJ851657_1(AJ851657|pid:none) Gallus gallus mRNA for hypothetica...   126   2e-27
AP005572_11(AP005572|pid:none) Oryza sativa Japonica Group genom...   125   3e-27
protein update 2009. 7.18
PSORT 

 psg: 0.76  gvh: 0.26  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHN330 is cyt
5' end seq. ID VHN330F
5' end seq. 
>VHN330F.Seq
ACAAAATGTGTAAATATAATGATCTAAATATTTAGTAGAAAAGGTAGATAGATTGGTATT
ACCAGAAAATGTTGTACCAATTAAATATGATTTACATTTAAAACCAAATTTAAAAGAATT
TACTTTTAAAGGTGAAGAGACCATCACAGTTCAAGTAAAACAACCAACAAAAACCATAAC
TATACATTCAATTGAAATTGAAATTCAATCAGCTTCAATAAAATCATCATCATCATCTCA
ATCATCAAAATCAATTACATTTTATGAACCAGAAGAAGTTGTCATCTTTGAATTTGAAAA
TGAATTATCAGTTGGTGAATATTGTTTATCATTGGTATTTACTGGTTTATTAAATGACAA
ATTAAAAGGTTTCTATCGTTCAAAGTATACTGTTAAAGGTGAAGATCGTTATTTAGCAAC
AACTCAATTCGAAGCCACCGATGCTCGTCGTTCTTTTCCATGTTTTGATGAACCAGCTCA
TAAAGCAGTTTTCAATATCACTTTAACTGTCTCTGAATGTCATACCGCTATTTCAAATAT
GGAAGAAAAATCAATTACTCCAAATAATGATGGTACTAAAACTTATATATTCGAACANNN
NNNNNNN
Length of 5' end seq. 607
3' end seq. ID VHN330Z
3' end seq. 
>VHN330Z.Seq
NNNNNNNNNNTAAGAAATAAAGTAAACTCTTATTTGGGTATTTTGGGTGACAAAGAGATT
GTGGCCGAGGCTAGAAAGAGATTTGAGCAATTCAAAGTCGACCAATCTTCTTTACCTTCC
GATATCCGTTCAAGTGTTTTAGTCACTGTTGTAAAGAATGGTTCCGAAGCTGAACAACAA
GAAATTATCAATAGATACCTCGCTTCCAATGATATTGCAGAGAAATCAAGTTTATTGTCA
GTTGTTTGCAAATCACCATCCAGTGCATTGGTATTGAAGGCTTTGGAATTCTCTGTCTCA
AAAGATGTTAGAACTTGTGAATCCTATATGCTTTGGAGAGTCGGAAATGAATTTAAACCA
GTAGTTTGGAAATATTTCACTGAAAACTTTAAATCAATCAATGAAACCTTTAATCAAAAT
GTTTTATTCGCTTATATGATCTCTTTTGCTTTGTCCTCAAAAATGACTGATCAACAATTA
CAACAAGTTGAAGATTTCTTCAAACAAAATCCAGTTGCAATTGCTGATCGTTCAATTAAA
CAAGATTTAGAACAAATTAGAAATAATACAAAATGGTTTATTCATTTAATAAAGATTTAT
TAAATGGATTAAAAAATAAATATCTATTAATAAAGATTTATAAATTGGATTTTANAAAAA
TAAAATTATTAATTTTATATTTTTTA
Length of 3' end seq. 686
Connected seq. ID VHN330P
Connected seq. 
>VHN330P.Seq
ACAAAATGTGTAAATATAATGATCTAAATATTTAGTAGAAAAGGTAGATAGATTGGTATT
ACCAGAAAATGTTGTACCAATTAAATATGATTTACATTTAAAACCAAATTTAAAAGAATT
TACTTTTAAAGGTGAAGAGACCATCACAGTTCAAGTAAAACAACCAACAAAAACCATAAC
TATACATTCAATTGAAATTGAAATTCAATCAGCTTCAATAAAATCATCATCATCATCTCA
ATCATCAAAATCAATTACATTTTATGAACCAGAAGAAGTTGTCATCTTTGAATTTGAAAA
TGAATTATCAGTTGGTGAATATTGTTTATCATTGGTATTTACTGGTTTATTAAATGACAA
ATTAAAAGGTTTCTATCGTTCAAAGTATACTGTTAAAGGTGAAGATCGTTATTTAGCAAC
AACTCAATTCGAAGCCACCGATGCTCGTCGTTCTTTTCCATGTTTTGATGAACCAGCTCA
TAAAGCAGTTTTCAATATCACTTTAACTGTCTCTGAATGTCATACCGCTATTTCAAATAT
GGAAGAAAAATCAATTACTCCAAATAATGATGGTACTAAAACTTATATATTCGAACA---
-------TAAGAAATAAAGTAAACTCTTATTTGGGTATTTTGGGTGACAAAGAGATTGTG
GCCGAGGCTAGAAAGAGATTTGAGCAATTCAAAGTCGACCAATCTTCTTTACCTTCCGAT
ATCCGTTCAAGTGTTTTAGTCACTGTTGTAAAGAATGGTTCCGAAGCTGAACAACAAGAA
ATTATCAATAGATACCTCGCTTCCAATGATATTGCAGAGAAATCAAGTTTATTGTCAGTT
GTTTGCAAATCACCATCCAGTGCATTGGTATTGAAGGCTTTGGAATTCTCTGTCTCAAAA
GATGTTAGAACTTGTGAATCCTATATGCTTTGGAGAGTCGGAAATGAATTTAAACCAGTA
GTTTGGAAATATTTCACTGAAAACTTTAAATCAATCAATGAAACCTTTAATCAAAATGTT
TTATTCGCTTATATGATCTCTTTTGCTTTGTCCTCAAAAATGACTGATCAACAATTACAA
CAAGTTGAAGATTTCTTCAAACAAAATCCAGTTGCAATTGCTGATCGTTCAATTAAACAA
GATTTAGAACAAATTAGAAATAATACAAAATGGTTTATTCATTTAATAAAGATTTATTAA
ATGGATTAAAAAATAAATATCTATTAATAAAGATTTATAAATTGGATTTTANAAAAATAA
AATTATTAATTTTATATTTTTTA
Length of connected seq. 1273
Full length Seq ID -
Full length Seq. -
Length of full length seq. -