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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO649
Library AF
(Link to library)
Clone ID AFO649
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15310-1
Original site URL
Representative seq. ID AFO649P
(Link to Original site)
Representative DNA sequence 
>AFO649 (AFO649Q) /CSM/AF/AFO6-C/AFO649Q.Seq.d/
AACTTAAATTAAAAATTATTATACCCCCTCACACTATATATAAATTTACATATATATAAA
AAAACACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGA
ACACTAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAG
ATACTGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCA
CCACCACCACCACCCCAACTCCAACTCCAACTCCAACACCAACTCCAACAACTACTACCA
CAACAGAAAAAAAAAGTAAAGAAATTGAAATTGTACCAACACCATCAAATGTACCAGCTT
CAATTTCAGCATCAACTTCAAATGTTGTAATTGCACCAGCTGTTGAAGCACAAATCACTG
ATGACTTTTCAGTTGTCCCAGAGAAAAATAAAAATAAAAAGAAGATCAATTCAACTGCAA
CTGATGGTACCACTACCACCACCAATATACCAAAACCAACACCAGTTAGAGCACCAATTA
CCAAAAAAGGTAACACTGCTCCACGTTCTCAATTCCATTTATTAGCACTCGACGAAGACG
ATATCACCTTTGATGAAACTXXXXXXXXXXAACAACCACTCAAAGAACAACCACTACTAA
ATCAACTCCAACTCCAACACCAACTCCAACTCCANNTGCTACTAAAGTTGTTGAACAACC
AAAAGAAAAATCATCACCAGCACCAGTTAAAAAAGAAAAAGAAATTNNTCAAAATAAAAA
AGAATCTGAAGGATTTTTATTCAGTTTAATGGAATCTTTAATTACTCCAGGTGTTCCATC
CGTAGTTTACAAATTCATTTACGTCGCCCTCATTGGTGTTTTATTATTTTCATTAGTACC
ATTATATTACTCTGGTTTAGATAGTATCTACAGTTATGGTGTCATCGCTTTAGTTTTAGG
TCTTGGTATTTCTTTAACTTTATTCATCAGTGAAATTCCAAGATTACAAGCTTCAAAGGA
ACAAAAATCAAAATCTGGTAACAAAAAATCAACNACTCGTAAAGTTANAGCATAAGNC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
lklkiiipphtiykftyi*KNTNTNKKFKMAGNKKQVKKNTKPIVVDIDDKPLLDTSNLD
TAVQTSASTKKDGKKVTATTTTTTPTPTPTPTPTPTTTTTTEKKSKEIEIVPTPSNVPAS
ISASTSNVVIAPAVEAQITDDFSVVPEKNKNKKKINSTATDGTTTTTNIPKPTPVRAPIT
KKGNTAPRSQFHLLALDEDDITFDET---

---TTTQRTTTTKSTPTPTPTPTPXATKVVEQPKEKSSPAPVKKEKEIXQNKKESEGFLF
SLMESLITPGVPSVVYKFIYVALIGVLLFSLVPLYYSGLDSIYSYGVIALVLGLGISLTL
FISEIPRLQASKEQKSKSGNKKSTTRKVXA*x
Translated Amino Acid sequence (All Frames) 
Frame A:
nln*kllypltlyinlhiykktqtqiknlkwqetrnklkrtlnqsllilminhy*ilqi*
ilqfkqvhqlkktekklllppppppqlqlqlqhqlqqllpqqkkkvkklklyqhhqmyql
qfqhqlqml*lhqllkhkslmtfqlsqrkikikrrsiqlqlmvplpppiyqnqhqlehql
pkkvtllhvlnsiy*hstktisplmk---

---nnhsknnhy*insnsntnsnsxcy*sc*ttkrkiitsts*krkrnxsk*kri*rifi
qfngifnysrcsirslqihlrrphwcfiifistiillwfr*ylqlwchrfsfrswyffnf
ihq*nskitsfkgtkikiw*qkinxs*sxsix

Frame B:
t*iknyytpshyi*iyiyikkhkhk*ki*ngrkqets*keh*tnrc*y***tiiryfkfr
ycssnkcin*krrkksycyhhhhhpnsnsnsntnsnnyyhnrkkk*rn*nctntikctsf
nfsinfkccnctsc*stnh**lfscprek*k*kedqfncn*wyhyhhqytktnts*stny
qkr*hcstfsipfistrrrryhl**n---

---TTTQRTTTTKSTPTPTPTPTPXATKVVEQPKEKSSPAPVKKEKEIXQNKKESEGFLF
SLMESLITPGVPSVVYKFIYVALIGVLLFSLVPLYYSGLDSIYSYGVIALVLGLGISLTL
FISEIPRLQASKEQKSKSGNKKSTTRKVXA*x

Frame C:
lklkiiipphtiykftyi*KNTNTNKKFKMAGNKKQVKKNTKPIVVDIDDKPLLDTSNLD
TAVQTSASTKKDGKKVTATTTTTTPTPTPTPTPTPTTTTTTEKKSKEIEIVPTPSNVPAS
ISASTSNVVIAPAVEAQITDDFSVVPEKNKNKKKINSTATDGTTTTTNIPKPTPVRAPIT
KKGNTAPRSQFHLLALDEDDITFDET---

---qplkeqpllnqlqlqhqlqlxxllkllnnqkknhhqhqlkkkkkxxkikknlkdfys
v*wnl*llqvfhp*ftnsftspslvfyyfh*yhyitlv*ivstvmvssl*f*vlvfl*ly
ssvkfqdyklqrnknqnlvtknqxlvklxhk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO649 (AFO649Q) /CSM/AF/AFO6-C/AFO649Q.Seq.d/                        573   e-162
SLE566 (SLE566Q) /CSM/SL/SLE5-C/SLE566Q.Seq.d/                        565   e-160
SLC662 (SLC662Q) /CSM/SL/SLC6-C/SLC662Q.Seq.d/                        565   e-160
SLB648 (SLB648Q) /CSM/SL/SLB6-B/SLB648Q.Seq.d/                        565   e-160
SLB638 (SLB638Q) /CSM/SL/SLB6-B/SLB638Q.Seq.d/                        565   e-160
SLA475 (SLA475Q) /CSM/SL/SLA4-D/SLA475Q.Seq.d/                        565   e-160
SFJ228 (SFJ228Q) /CSM/SF/SFJ2-B/SFJ228Q.Seq.d/                        565   e-160
SFI764 (SFI764Q) /CSM/SF/SFI7-C/SFI764Q.Seq.d/                        565   e-160
SFI220 (SFI220Q) /CSM/SF/SFI2-A/SFI220Q.Seq.d/                        565   e-160
SFG629 (SFG629Q) /CSM/SF/SFG6-B/SFG629Q.Seq.d/                        565   e-160
own update 2001.11.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL451012|AL451012.1 Neurospora crassa DNA linkage group II BAC clone B21O8.    58  2e-04  1
AL672276|AL672276.9 Mouse DNA sequence from clone RP23-124L1 on chromosome 4.    56  0.001  1
AW490807|AW490807.1 UI-M-BH3-asz-d-01-0-UI.s1 NIH_BMAP_M_S4 Mus musculus cDNA clone UI-M-BH3-asz-d-01-0-UI 3', mRNA sequence.    56  0.001  1
BB641856|BB641856.1 Mus musculus 10 days neonate cortex cDNA, RIKEN full-length enriched library, clone:A830059M14, 5' end partial sequence.    56  0.001  1
BE651335|BE651335.1 UI-M-BH3-asz-d-01-0-UI.r1 NIH_BMAP_M_S4 Mus musculus cDNA clone UI-M-BH3-asz-d-01-0-UI 5', mRNA sequence.    56  0.001  1
BQ747766|BQ747766.1 UI-M-FA0-bxr-d-02-0-UI.r1 NIH_BMAP_FA0 Mus musculus cDNA clone IMAGE:5711953 5', mRNA sequence.    56  0.001  1
BU604682|BU604682.1 AGENCOURT_10050672 NIH_MGC_144 Mus musculus cDNA clone IMAGE:6535829 5', mRNA sequence.    56  0.001  1
AF465309|AF465309.1 Dictyostelium discoideum ABC transporter ABCA.7 (abca7) gene, complete cds.    56  0.001  1
CF542178|CF542178.1 CsC029F cDNA library of cucumber cultivar Addis Cucumis sativus cDNA clone CsC029F, mRNA sequence.    56  0.001  1
CF542168|CF542168.1 CsC029R cDNA library of cucumber cultivar Addis Cucumis sativus cDNA clone CsC029R, mRNA sequence.    56  0.001  1
dna update 2003. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54SG3) RecName: Full=Uncharacterized transmembrane protein DDB...   230   7e-59
EF535298_1(EF535298|pid:none) Platemys platycephala isolate AK10...    38   0.66
EF535297_1(EF535297|pid:none) Platemys platycephala isolate AK10...    38   0.66
EF535299_1(EF535299|pid:none) Platemys platycephala isolate AK66...    37   0.87
AP009179_1281(AP009179|pid:none) Sulfurovum sp. NBC37-1 genomic ...    37   1.1
EF535301_1(EF535301|pid:none) Acanthochelys radiolata isolate AK...    37   1.5
AM295250_1660(AM295250|pid:none) Staphylococcus carnosus subsp. ...    36   1.9
EF362619_2(EF362619|pid:none) Lactococcus lactis subsp. lactis b...    34   7.3
EU880323_10(EU880323|pid:none) Notophthalmus viridescens mitocho...    34   7.3
(P21608) RecName: Full=Citrate-sodium symporter; AltName: Full=C...    34   7.3
protein update 2009. 4.26
PSORT 

 psg: 0.57  gvh: 0.42  alm: 0.20  top: 0.47  tms: 0.13  mit: 0.30  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 44.0 %: endoplasmic reticulum
	 24.0 %: plasma membrane
	 12.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system
	  4.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for AFO649 is end
5' end seq. ID AFO649F
5' end seq. 
>AFO649F.Seq
AACTTAAATTAAAAATTATTATACCCCCTCACACTATATATAAATTTACATATATATAAA
AAAACACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGA
ACACTAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAG
ATACTGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCA
CCACCACCACCACCCCAACTCCAACTCCAACTCCAACACCAACTCCAACAACTACTACCA
CAACAGAAAAAAAAAGTAAAGAAATTGAAATTGTACCAACACCATCAAATGTACCAGCTT
CAATTTCAGCATCAACTTCAAATGTTGTAATTGCACCAGCTGTTGAAGCACAAATCACTG
ATGACTTTTCAGTTGTCCCAGAGAAAAATAAAAATAAAAAGAAGATCAATTCAACTGCAA
CTGATGGTACCACTACCACCACCAATATACCAAAACCAACACCAGTTAGAGCACCAATTA
CCAAAAAAGGTAACACTGCTCCACGTTCTCAATTCCATTTATTAGCACTCGACGAAGACG
ATATCACCTTTGATGAAACT----------
Length of 5' end seq. 620
3' end seq. ID AFO649Z
3' end seq. 
>AFO649Z.Seq
----------AACAACCACTCAAAGAACAACCACTACTAAATCAACTCCAACTCCAACAC
CAACTCCAACTCCANNTGCTACTAAAGTTGTTGAACAACCAAAAGAAAAATCATCACCAG
CACCAGTTAAAAAAGAAAAAGAAATTNNTCAAAATAAAAAAGAATCTGAAGGATTTTTAT
TCAGTTTAATGGAATCTTTAATTACTCCAGGTGTTCCATCCGTAGTTTACAAATTCATTT
ACGTCGCCCTCATTGGTGTTTTATTATTTTCATTAGTACCATTATATTACTCTGGTTTAG
ATAGTATCTACAGTTATGGTGTCATCGCTTTAGTTTTAGGTCTTGGTATTTCTTTAACTT
TATTCATCAGTGAAATTCCAAGATTACAAGCTTCAAAGGAACAAAAATCAAAATCTGGTA
ACAAAAAATCAACNACTCGTAAAGTTANAGCATAAGNC
Length of 3' end seq. 448
Connected seq. ID AFO649P
Connected seq. 
>AFO649P.Seq
AACTTAAATTAAAAATTATTATACCCCCTCACACTATATATAAATTTACATATATATAAA
AAAACACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGA
ACACTAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAG
ATACTGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCA
CCACCACCACCACCCCAACTCCAACTCCAACTCCAACACCAACTCCAACAACTACTACCA
CAACAGAAAAAAAAAGTAAAGAAATTGAAATTGTACCAACACCATCAAATGTACCAGCTT
CAATTTCAGCATCAACTTCAAATGTTGTAATTGCACCAGCTGTTGAAGCACAAATCACTG
ATGACTTTTCAGTTGTCCCAGAGAAAAATAAAAATAAAAAGAAGATCAATTCAACTGCAA
CTGATGGTACCACTACCACCACCAATATACCAAAACCAACACCAGTTAGAGCACCAATTA
CCAAAAAAGGTAACACTGCTCCACGTTCTCAATTCCATTTATTAGCACTCGACGAAGACG
ATATCACCTTTGATGAAACT----------AACAACCACTCAAAGAACAACCACTACTAA
ATCAACTCCAACTCCAACACCAACTCCAACTCCANNTGCTACTAAAGTTGTTGAACAACC
AAAAGAAAAATCATCACCAGCACCAGTTAAAAAAGAAAAAGAAATTNNTCAAAATAAAAA
AGAATCTGAAGGATTTTTATTCAGTTTAATGGAATCTTTAATTACTCCAGGTGTTCCATC
CGTAGTTTACAAATTCATTTACGTCGCCCTCATTGGTGTTTTATTATTTTCATTAGTACC
ATTATATTACTCTGGTTTAGATAGTATCTACAGTTATGGTGTCATCGCTTTAGTTTTAGG
TCTTGGTATTTCTTTAACTTTATTCATCAGTGAAATTCCAAGATTACAAGCTTCAAAGGA
ACAAAAATCAAAATCTGGTAACAAAAAATCAACNACTCGTAAAGTTANAGCATAAGNC
Length of connected seq. 1068
Full length Seq ID -
Full length Seq. -
Length of full length seq. -