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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHN252
Library VH
(Link to library)
Clone ID VHN252
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15690-1
Original site URL
Representative seq. ID VHN252P
(Link to Original site)
Representative DNA sequence 
>VHN252 (VHN252Q) /CSM/VH/VHN2-C/VHN252Q.Seq.d/
AAACGTAATTTAGTTNGTGAAGTTAGAAGAAATCNTACATTAGAAAGAGATCTTAATCGT
CTTGATAAGAGAATTGCACTTTTAATTAAAAATCGTGGTAATATTCAAGATGTATTAGCA
GACAAAGCTGGTCTTAAAGCACCAAAACATAAGGGAGATCAAAAGAAACCAGAGCTTATT
AATGATCCAAAGAAATTAGAAACCTATCAAAATCTCTTTTACTTACTTCAAACAGAACCA
AAATATTTGGCAGGTTTGGTTTATTTGATTCAACCAGAGCAAATGGAGTCCTTCTTGGGT
ACCGTCATCTTGACATTGTTTGGTGATGCATTCACACCAAGAGAAGAGTTTCTCTTACTC
TCTCTCTATAGACTTTCAATTCAAAAGGNGATGGCAAACATTGCAACCGTTGGTGATTTC
CTCAAGGCTGACACTGTCGTTCCAAAGATGATTATCACCTACAACAAACGTAAACAAGGT
ACCGACTAXXXXXXXXXXCTTGTCGATGTTTTAAACAAGGCTCGTCAAGCTGATCCATCT
TTGGAGCCAGAAATCAAAAAGATCCTTGATAACCTCAAAAAACTTGAAGAATATAATCTC
ACCACCTCTGCCGATAATTATTCCTCTTTCCTCAAGGCAGTCGCACTCGAAGTAGTTAAT
CGTGCTGAAATTCGTGAACAACAAAAGAAGGAGAAACAAAGATTAACCACCTCTTTGAAT
AATCTTCGTAAACATCAAAAATATCTCAACGAACAAATCGCTCAATACAATCAATACCTC
CAAGATTGTCGTCTCAAACATTATCAAAACAAGAGCAAAAAGAAGAAGAAGGGAGATGGT
GCCAAAGTTGGTCCTTTCAAATTCAGTTTCAGCGAACTTCACAAGAAAGGTGTCATCGTC
GACTCTGAAGTTCCTCAAATCACCAGAAAGAAGATTAAATTTGTCATTAGCTCTGATACC
GTTGGTGTGTTTGATGTCTCTGCTAAAATGGCTGGTATCGATGTTCAAACTATGCGTTTG
GAACTTGATGACCTTTTAGAACTCAATTCAATTGGCACCACAACTTTAGAATTAGATCAA
ATCACTTTAGATGTTAATATGACCATACATTTATTAAACAAACTTTTCTTATA
sequence update 2002.10.25
Translated Amino Acid sequence 
KRNLVXEVRRNXTLERDLNRLDKRIALLIKNRGNIQDVLADKAGLKAPKHKGDQKKPELI
NDPKKLETYQNLFYLLQTEPKYLAGLVYLIQPEQMESFLGTVILTLFGDAFTPREEFLLL
SLYRLSIQKXMANIATVGDFLKADTVVPKMIITYNKRKQGTD---

---LVDVLNKARQADPSLEPEIKKILDNLKKLEEYNLTTSADNYSSFLKAVALEVVNRAE
IREQQKKEKQRLTTSLNNLRKHQKYLNEQIAQYNQYLQDCRLKHYQNKSKKKKKGDGAKV
GPFKFSFSELHKKGVIVDSEVPQITRKKIKFVISSDTVGVFDVSAKMAGIDVQTMRLELD
DLLELNSIGTTTLELDQITLDVNMTIHLLNKLFL
Translated Amino Acid sequence (All Frames) 
Frame A:
KRNLVXEVRRNXTLERDLNRLDKRIALLIKNRGNIQDVLADKAGLKAPKHKGDQKKPELI
NDPKKLETYQNLFYLLQTEPKYLAGLVYLIQPEQMESFLGTVILTLFGDAFTPREEFLLL
SLYRLSIQKXMANIATVGDFLKADTVVPKMIITYNKRKQGTD---

---LVDVLNKARQADPSLEPEIKKILDNLKKLEEYNLTTSADNYSSFLKAVALEVVNRAE
IREQQKKEKQRLTTSLNNLRKHQKYLNEQIAQYNQYLQDCRLKHYQNKSKKKKKGDGAKV
GPFKFSFSELHKKGVIVDSEVPQITRKKIKFVISSDTVGVFDVSAKMAGIDVQTMRLELD
DLLELNSIGTTTLELDQITLDVNMTIHLLNKLFL

Frame B:
nvi*xvkleeixh*keilivlirelhf*lkivvifkmy*qtklvlkhqnireikrnqsll
miqrn*kpikisftyfkqnqniwqvwfi*fnqskwspswvpss*hclvmhshqeksfsys
lsidfqfkrxwqtlqplvissrltlsfqr*lspttnvnkvpt---

---lsmf*trlvklihlwsqkskrslitsknlkniispplpiiiplssrqshsk*livlk
fvnnkrrrnkd*ppl*iifvniknistnkslntintskivvsniiktrakrrrremvpkl
vlsnsvsanftrkvssstlkflksperrlnlslaliplvclmsllkwlvsmfklcvwnlm
tf*nsiqlapql*n*iksl*mli*pyiy*tnfsy

Frame C:
t*fsx*s*kksyirkrs*ss**enctfn*ksw*ysrcisrqsws*stkt*grsketray*
*skeirnlsksllltsnrtkifgrfglfdstrangvllgyrhldivw*cihtkrrvsltl
sl*tfnskgdgkhcnrw*fpqg*hcrskddyhlqqt*tryrl---

---crcfkqgsss*sifgarnqkdp**pqkt*ri*shhlcr*lflfpqgsrtrss*sc*n
s*ttkegetkinhlfe*ss*tskisqrtnrsiqsipprlssqtlskqeqkeeegrwcqsw
sfqiqfqrtsqerchrrl*sssnhqked*ich*l*yrwcv*clc*ngwyrcsnyafgt**
pfrtqfnwhhnfrirsnhfrc*ydhtfikqtfli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHN252 (VHN252Q) /CSM/VH/VHN2-C/VHN252Q.Seq.d/                       2169   0.0
VHH220 (VHH220Q) /CSM/VH/VHH2-A/VHH220Q.Seq.d/                       1259   0.0
SHL214 (SHL214Q) /CSM/SH/SHL2-A/SHL214Q.Seq.d/                       1259   0.0
SHL120 (SHL120Q) /CSM/SH/SHL1-A/SHL120Q.Seq.d/                       1259   0.0
SHK836 (SHK836Q) /CSM/SH/SHK8-B/SHK836Q.Seq.d/                       1259   0.0
SHK407 (SHK407Q) /CSM/SH/SHK4-A/SHK407Q.Seq.d/                       1259   0.0
SHD812 (SHD812Q) /CSM/SH/SHD8-A/SHD812Q.Seq.d/                       1259   0.0
SHC344 (SHC344Q) /CSM/SH/SHC3-B/SHC344Q.Seq.d/                       1259   0.0
SHA237 (SHA237Q) /CSM/SH/SHA2-B/SHA237Q.Seq.d/                       1259   0.0
CHM205 (CHM205Q) /CSM/CH/CHM2-A/CHM205Q.Seq.d/                       1259   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U81156) Dictyostelium discoideum RasGAP1 (rgaA) gene, compl...  1259   0.0    1
(L75794) Dictyostelium discoideum RasGTPase-activating prote...  1259   0.0    1
(BJ444827) Dictyostelium discoideum cDNA clone:ddv57g14, 3' ...  1259   0.0    1
(BJ440903) Dictyostelium discoideum cDNA clone:ddv45g06, 3' ...  1259   0.0    1
(BJ408534) Dictyostelium discoideum cDNA clone:dds46k04, 3' ...  1259   0.0    1
(BJ408515) Dictyostelium discoideum cDNA clone:dds46g05, 3' ...  1259   0.0    1
(BJ408292) Dictyostelium discoideum cDNA clone:dds45h10, 3' ...  1259   0.0    1
(BJ407950) Dictyostelium discoideum cDNA clone:dds44n02, 3' ...  1259   0.0    1
(BJ405257) Dictyostelium discoideum cDNA clone:dds31h04, 3' ...  1259   0.0    1
(BJ404378) Dictyostelium discoideum cDNA clone:dds28h11, 3' ...  1259   0.0    1
dna update 2008.12. 4
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U81156_1(U81156|pid:none) Dictyostelium discoideum RasGAP1 (rgaA...   367   e-100
AK302981_1(AK302981|pid:none) Homo sapiens cDNA FLJ52376 complet...   112   2e-23
AK303054_1(AK303054|pid:none) Homo sapiens cDNA FLJ50398 complet...   112   2e-23
(Q13576) RecName: Full=Ras GTPase-activating-like protein IQGAP2...   112   2e-23
AY351902_1(AY351902|pid:none) Homo sapiens testis specific IQ mo...   112   2e-23
BC150958_1(BC150958|pid:none) Mus musculus IQ motif containing G...   112   2e-23
AK042038_1(AK042038|pid:none) Mus musculus 3 days neonate thymus...   112   2e-23
AK142813_1(AK142813|pid:none) Mus musculus 15 days embryo head c...   112   2e-23
(Q3UQ44) RecName: Full=Ras GTPase-activating-like protein IQGAP2;     112   2e-23
AK302510_1(AK302510|pid:none) Homo sapiens cDNA FLJ51769 partial...   111   5e-23
protein update 2009. 7.18
PSORT 

 psg: 0.47  gvh: 0.33  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.02
 nuc: 0.18  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial

>> prediction for VHN252 is nuc
5' end seq. ID VHN252F
5' end seq. 
>VHN252F.Seq
AAACGTAATTTAGTTNGTGAAGTTAGAAGAAATCNTACATTAGAAAGAGATCTTAATCGT
CTTGATAAGAGAATTGCACTTTTAATTAAAAATCGTGGTAATATTCAAGATGTATTAGCA
GACAAAGCTGGTCTTAAAGCACCAAAACATAAGGGAGATCAAAAGAAACCAGAGCTTATT
AATGATCCAAAGAAATTAGAAACCTATCAAAATCTCTTTTACTTACTTCAAACAGAACCA
AAATATTTGGCAGGTTTGGTTTATTTGATTCAACCAGAGCAAATGGAGTCCTTCTTGGGT
ACCGTCATCTTGACATTGTTTGGTGATGCATTCACACCAAGAGAAGAGTTTCTCTTACTC
TCTCTCTATAGACTTTCAATTCAAAAGGNGATGGCAAACATTGCAACCGTTGGTGATTTC
CTCAAGGCTGACACTGTCGTTCCAAAGATGATTATCACCTACAACAAACGTAAACAAGGT
ACCGACTANNNNNNNNNN
Length of 5' end seq. 498
3' end seq. ID VHN252Z
3' end seq. 
>VHN252Z.Seq
NNNNNNNNNNCTTGTCGATGTTTTAAACAAGGCTCGTCAAGCTGATCCATCTTTGGAGCC
AGAAATCAAAAAGATCCTTGATAACCTCAAAAAACTTGAAGAATATAATCTCACCACCTC
TGCCGATAATTATTCCTCTTTCCTCAAGGCAGTCGCACTCGAAGTAGTTAATCGTGCTGA
AATTCGTGAACAACAAAAGAAGGAGAAACAAAGATTAACCACCTCTTTGAATAATCTTCG
TAAACATCAAAAATATCTCAACGAACAAATCGCTCAATACAATCAATACCTCCAAGATTG
TCGTCTCAAACATTATCAAAACAAGAGCAAAAAGAAGAAGAAGGGAGATGGTGCCAAAGT
TGGTCCTTTCAAATTCAGTTTCAGCGAACTTCACAAGAAAGGTGTCATCGTCGACTCTGA
AGTTCCTCAAATCACCAGAAAGAAGATTAAATTTGTCATTAGCTCTGATACCGTTGGTGT
GTTTGATGTCTCTGCTAAAATGGCTGGTATCGATGTTCAAACTATGCGTTTGGAACTTGA
TGACCTTTTAGAACTCAATTCAATTGGCACCACAACTTTAGAATTAGATCAAATCACTTT
AGATGTTAATATGACCATACATTTATTAAACAAACTTTTCTTATA
Length of 3' end seq. 645
Connected seq. ID VHN252P
Connected seq. 
>VHN252P.Seq
AAACGTAATTTAGTTNGTGAAGTTAGAAGAAATCNTACATTAGAAAGAGATCTTAATCGT
CTTGATAAGAGAATTGCACTTTTAATTAAAAATCGTGGTAATATTCAAGATGTATTAGCA
GACAAAGCTGGTCTTAAAGCACCAAAACATAAGGGAGATCAAAAGAAACCAGAGCTTATT
AATGATCCAAAGAAATTAGAAACCTATCAAAATCTCTTTTACTTACTTCAAACAGAACCA
AAATATTTGGCAGGTTTGGTTTATTTGATTCAACCAGAGCAAATGGAGTCCTTCTTGGGT
ACCGTCATCTTGACATTGTTTGGTGATGCATTCACACCAAGAGAAGAGTTTCTCTTACTC
TCTCTCTATAGACTTTCAATTCAAAAGGNGATGGCAAACATTGCAACCGTTGGTGATTTC
CTCAAGGCTGACACTGTCGTTCCAAAGATGATTATCACCTACAACAAACGTAAACAAGGT
ACCGACTA----------CTTGTCGATGTTTTAAACAAGGCTCGTCAAGCTGATCCATCT
TTGGAGCCAGAAATCAAAAAGATCCTTGATAACCTCAAAAAACTTGAAGAATATAATCTC
ACCACCTCTGCCGATAATTATTCCTCTTTCCTCAAGGCAGTCGCACTCGAAGTAGTTAAT
CGTGCTGAAATTCGTGAACAACAAAAGAAGGAGAAACAAAGATTAACCACCTCTTTGAAT
AATCTTCGTAAACATCAAAAATATCTCAACGAACAAATCGCTCAATACAATCAATACCTC
CAAGATTGTCGTCTCAAACATTATCAAAACAAGAGCAAAAAGAAGAAGAAGGGAGATGGT
GCCAAAGTTGGTCCTTTCAAATTCAGTTTCAGCGAACTTCACAAGAAAGGTGTCATCGTC
GACTCTGAAGTTCCTCAAATCACCAGAAAGAAGATTAAATTTGTCATTAGCTCTGATACC
GTTGGTGTGTTTGATGTCTCTGCTAAAATGGCTGGTATCGATGTTCAAACTATGCGTTTG
GAACTTGATGACCTTTTAGAACTCAATTCAATTGGCACCACAACTTTAGAATTAGATCAA
ATCACTTTAGATGTTAATATGACCATACATTTATTAAACAAACTTTTCTTATA
Length of connected seq. 1123
Full length Seq ID -
Full length Seq. -
Length of full length seq. -