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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO643
Library AF
(Link to library)
Clone ID AFO643
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15316-1
Original site URL
Representative seq. ID AFO643P
(Link to Original site)
Representative DNA sequence 
>AFO643 (AFO643Q) /CSM/AF/AFO6-B/AFO643Q.Seq.d/
ATTTTTTTAAAAAAAAGAAATGAATGGAGTTGATATAAATTCAAATTTAGATTCAATTAA
AGCAAAAGCAGAAGATATTAGAAATTTATTATTAACAACTTCAACTGGTAATTGGGAGGA
TATTTTAGGACATTTTGTAGTATTATCAGGTGAATATGGATCATTAGTCAGAGAGGTCAA
TAATATATACGATAGTGTTGCAGTATTTCCTGAACAAGTTACAGGTGATCCAAATTTAAT
ACCAAATTTATTAAGAACAAAACTATCACCAGAATTGGAGACCAAGAATAGAGATATCAT
AAATCAATACGTTTCAAAGAATTCCACTCACTATGATCCTGGAAATCAAGAGGAGAATAT
ACCACCATTGAAATTACATATAAAATCATTCAATGATAGAATAGATAAAGTGGATCAAGA
ATTTGAGAATAAAATTCAACAAATCAGTAAACATAAATTAACCAAACCATCAGCATCCCA
CGATACCGCAATCCTCAAAGACATCCTCTCCATCACAATGCTAGGATCAGGTATTAAAAT
CAATGCTCAACAACAATTAGCACAACAACAACAACAACAAGCTACAATCAATGCATTACA
ACAACAACAAAGATTATTAAATGCACAACAACAACAACAACAGCAACAACAACCACAAGC
ATTAXXXXXXXXXXACCNACAGCANCAGTATCANNTGCTGCCACTTATAAACCAGTGGCA
TCACCACAACAAACAAGTTACTTCTAAGCAAGTNCCAATACAGTCAACAAATAAACCATT
ACCACAACAACAAACCTTCAATACAAATTCAACCACAACAATCTATTGTGCAAATGGTGC
AGAATGTTTTACCAAATACAACTTCACCGCCTGTTAACAATAATAATCAATCACCAATTA
ATAGTGGTATTGGAGGAGGTCAATTTAAACCATCAGCTATTAATATTGCAAATGCAAATA
GTGGTACATCAACAATTCAACAACAAATTCAATTACAACAACAACAATTACAACAACAAA
TTCAATTACAACAACAATTAGCACAGCAGCAACAACAAAATCAACAAAATCAACAAAATC
AACAAAACCAGCAAAACCAACAAAATCAACAAATTCATCAACATATTCAACATTTAACAC
CACAACAACAAGCTCAATTACAACAACAAATGTTACAACAACAACAACTTCAACAACAAT
TTCAACAACAACAATTATCACAACAACTTTTACAACAACAACAACTTCATCAACAACAAC
AACCAAATCAAGACAATTCAAAATCCATTTCATAATTTTCAAGCAAATAATTTCAGAGTT
CAAAATCCTACAAATTTTATCCACCACAAAATCAATAAAAGTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ff*KKEMNGVDINSNLDSIKAKAEDIRNLLLTTSTGNWEDILGHFVVLSGEYGSLVREVN
NIYDSVAVFPEQVTGDPNLIPNLLRTKLSPELETKNRDIINQYVSKNSTHYDPGNQEENI
PPLKLHIKSFNDRIDKVDQEFENKIQQISKHKLTKPSASHDTAILKDILSITMLGSGIKI
NAQQQLAQQQQQQATINALQQQQRLLNAQQQQQQQQQPQAL---

---XQXQYXXLPLINQWHHHNKQVTSKQVPIQSTNKPLPQQQTFNTNSTTTIYCANGAEC
FTKYNFTAC*q**sitn**wywrrsi*tisy*yckck*wyinnsttnsittttitttnsi
tttistaattkstkstkstkpakptkstnsstystfnttttssitttnvtttttsttist
ttiitttftttttssttttksrqfkihfiifkqiisefkilqilsttksikv
Translated Amino Acid sequence (All Frames) 
Frame A:
iflkkrnews*ykfkfrfn*sksrry*kfiinnfnw*lggyfrtfcsiir*iwiisqrgq
*yir*ccsis*tsyr*skfntkfiknktitrigdqe*ryhksirfkefhsl*swksrgey
ttieitykiiq**nr*sgsri*e*nstnq*t*inqtisipryrnpqrhplhhnariry*n
qcsttisttttttsynqcittttkiikcttttttattttsi---

---txsxsixcchl*tsgitttnklllskxqysqqinhyhnnkpsiqiqpqqsivqmvqn
vlpnttsppvnnnnqspinsgigggqfkpsainianansgtstiqqqiqlqqqqlqqqiq
lqqqlaqqqqqnqqnqqnqqnqqnqqnqqihqhiqhltpqqqaqlqqqmlqqqqlqqqfq
qqqlsqqllqqqqlhqqqqpnqdnsksis*fssk*fqssksykfyppqnq*k

Frame B:
ff*KKEMNGVDINSNLDSIKAKAEDIRNLLLTTSTGNWEDILGHFVVLSGEYGSLVREVN
NIYDSVAVFPEQVTGDPNLIPNLLRTKLSPELETKNRDIINQYVSKNSTHYDPGNQEENI
PPLKLHIKSFNDRIDKVDQEFENKIQQISKHKLTKPSASHDTAILKDILSITMLGSGIKI
NAQQQLAQQQQQQATINALQQQQRLLNAQQQQQQQQQPQAL---

---ptaxvsxaatykpvaspqqtsyf*asxntvnk*tittttnlqykfnhnnllckwcrm
fyqiqlhrlltiiinhqlivvleevnlnhqllilqmqivvhqqfnnkfnynnnnynnkfn
ynnn*hssnnkinkinkinktsktnkinkfinifni*hhnnklnynnkcynnnnfnnnfn
nnnyhnnfynnnnfinnnnqiktiqnpfhnfqannfrvqnptnfihhkinks

Frame C:
ffkkkk*meli*iqi*iqlkqkqkileiyy*qlqlvigrif*dil*yyqvnmdh*sersi
iytivlqyflnklqviqi*yqiy*eqnyhqnwrprieis*intfqripltmileikrriy
hh*nyi*nhsmie*ikwiknlrikfnksvnin*pnhqhptipqssktsspsqc*dqvlks
mlnnn*hnnnnnklqsmhynnnkdy*mhnnnnnsnnnhkh---

---XQXQYXXLPLINQWHHHNKQVTSKQVPIQSTNKPLPQQQTFNTNSTTTIYCANGAEC
FTKYNFTAC*q**sitn**wywrrsi*tisy*yckck*wyinnsttnsittttitttnsi
tttistaattkstkstkstkpakptkstnsstystfnttttssitttnvtttttsttist
ttiitttftttttssttttksrqfkihfiifkqiisefkilqilsttksikv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFN355 (VFN355Q) /CSM/VF/VFN3-C/VFN355Q.Seq.d/                       1053   0.0
CFC365 (CFC365Q) /CSM/CF/CFC3-C/CFC365Q.Seq.d/                       1053   0.0
AFO643 (AFO643Q) /CSM/AF/AFO6-B/AFO643Q.Seq.d/                       1053   0.0
AFI817 (AFI817Q) /CSM/AF/AFI8-A/AFI817Q.Seq.d/                       1053   0.0
AFB455 (AFB455Q) /CSM/AF/AFB4-C/AFB455Q.Seq.d/                       1053   0.0
AFM704 (AFM704Q) /CSM/AF/AFM7-A/AFM704Q.Seq.d/                        936   0.0
CFK186 (CFK186Q) /CSM/CF/CFK1-D/CFK186Q.Seq.d/                        468   e-130
VFE795 (VFE795Q) /CSM/VF/VFE7-D/VFE795Q.Seq.d/                        291   1e-77
VSJ264 (VSJ264Q) /CSM/VS/VSJ2-C/VSJ264Q.Seq.d/                         40   0.087
VSE351 (VSE351Q) /CSM/VS/VSE3-C/VSE351Q.Seq.d/                         40   0.087
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    32  0.068  7
AZ675437|AZ675437.1 ENTMM30TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    38  0.18  2
AC007295|AC007295.8 Arabidopsis thaliana chromosome 2 clone T4E5 map PR1, complete sequence.    38  0.88  5
BH209864|BH209864.1 Sm1-57G17.TR Sm1 Schistosoma mansoni genomic clone Sm1-57G17, DNA sequence.    46  1.2  1
BH132177|BH132177.1 ENTNR63TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    46  1.2  1
AY230875|AY230875.1 Phragmites australis clone PaGT21 microsatellite sequence.    34  2.1  2
AC145770|AC145770.1 Pan troglodytes BAC clone CH251-576K13 from Y, complete sequence.    40  2.4  3
AL645508|AL645508.14 Mouse DNA sequence from clone RP23-170H12 on chromosome 4.    38  3.1  3
Z78067|Z78067.1 Caenorhabditis elegans cosmid ZC412.              38  4.4  4
BH421452|BH421452.1 BOGKK54TF BOGK Brassica oleracea genomic clone BOGKK54, DNA sequence.    44  4.6  1
dna update 2003. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK229348_1(AK229348|pid:none) Arabidopsis thaliana mRNA for hypo...    50   2e-04
EF678671_1(EF678671|pid:none) Picea sitchensis clone WS02930_K02...    45   0.006
EU976541_1(EU976541|pid:none) Zea mays clone 981034 unknown mRNA.      41   0.083
CP000743_812(CP000743|pid:none) Methanococcus aeolicus Nankai-3,...    38   0.70
EF568108_38(EF568108|pid:none) Glossina pallidipes salivary glan...    37   1.2
FM992695_197(FM992695|pid:none) Candida dubliniensis CD36 chromo...    36   2.7
CP001034_1349(CP001034|pid:none) Natranaerobius thermophilus JW/...    36   2.7
(Q5AMN3) RecName: Full=Autophagy-related protein 11;                   36   2.7
AE014187_181(AE014187|pid:none) Plasmodium falciparum 3D7 chromo...    36   3.5
CP001078_2659(CP001078|pid:none) Clostridium botulinum E3 str. A...    35   4.5
protein update 2009. 4.26
PSORT 

 psg: 0.67  gvh: 0.40  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for AFO643 is cyt
5' end seq. ID AFO643F
5' end seq. 
>AFO643F.Seq
ATTTTTTTAAAAAAAAGAAATGAATGGAGTTGATATAAATTCAAATTTAGATTCAATTAA
AGCAAAAGCAGAAGATATTAGAAATTTATTATTAACAACTTCAACTGGTAATTGGGAGGA
TATTTTAGGACATTTTGTAGTATTATCAGGTGAATATGGATCATTAGTCAGAGAGGTCAA
TAATATATACGATAGTGTTGCAGTATTTCCTGAACAAGTTACAGGTGATCCAAATTTAAT
ACCAAATTTATTAAGAACAAAACTATCACCAGAATTGGAGACCAAGAATAGAGATATCAT
AAATCAATACGTTTCAAAGAATTCCACTCACTATGATCCTGGAAATCAAGAGGAGAATAT
ACCACCATTGAAATTACATATAAAATCATTCAATGATAGAATAGATAAAGTGGATCAAGA
ATTTGAGAATAAAATTCAACAAATCAGTAAACATAAATTAACCAAACCATCAGCATCCCA
CGATACCGCAATCCTCAAAGACATCCTCTCCATCACAATGCTAGGATCAGGTATTAAAAT
CAATGCTCAACAACAATTAGCACAACAACAACAACAACAAGCTACAATCAATGCATTACA
ACAACAACAAAGATTATTAAATGCACAACAACAACAACAACAGCAACAACAACCACAAGC
ATTA----------
Length of 5' end seq. 664
3' end seq. ID AFO643Z
3' end seq. 
>AFO643Z.Seq
----------ACCNACAGCANCAGTATCANNTGCTGCCACTTATAAACCAGTGGCATCAC
CACAACAAACAAGTTACTTCTAAGCAAGTNCCAATACAGTCAACAAATAAACCATTACCA
CAACAACAAACCTTCAATACAAATTCAACCACAACAATCTATTGTGCAAATGGTGCAGAA
TGTTTTACCAAATACAACTTCACCGCCTGTTAACAATAATAATCAATCACCAATTAATAG
TGGTATTGGAGGAGGTCAATTTAAACCATCAGCTATTAATATTGCAAATGCAAATAGTGG
TACATCAACAATTCAACAACAAATTCAATTACAACAACAACAATTACAACAACAAATTCA
ATTACAACAACAATTAGCACAGCAGCAACAACAAAATCAACAAAATCAACAAAATCAACA
AAACCAGCAAAACCAACAAAATCAACAAATTCATCAACATATTCAACATTTAACACCACA
ACAACAAGCTCAATTACAACAACAAATGTTACAACAACAACAACTTCAACAACAATTTCA
ACAACAACAATTATCACAACAACTTTTACAACAACAACAACTTCATCAACAACAACAACC
AAATCAAGACAATTCAAAATCCATTTCATAATTTTCAAGCAAATAATTTCAGAGTTCAAA
ATCCTACAAATTTTATCCACCACAAAATCAATAAAAGTA
Length of 3' end seq. 689
Connected seq. ID AFO643P
Connected seq. 
>AFO643P.Seq
ATTTTTTTAAAAAAAAGAAATGAATGGAGTTGATATAAATTCAAATTTAGATTCAATTAA
AGCAAAAGCAGAAGATATTAGAAATTTATTATTAACAACTTCAACTGGTAATTGGGAGGA
TATTTTAGGACATTTTGTAGTATTATCAGGTGAATATGGATCATTAGTCAGAGAGGTCAA
TAATATATACGATAGTGTTGCAGTATTTCCTGAACAAGTTACAGGTGATCCAAATTTAAT
ACCAAATTTATTAAGAACAAAACTATCACCAGAATTGGAGACCAAGAATAGAGATATCAT
AAATCAATACGTTTCAAAGAATTCCACTCACTATGATCCTGGAAATCAAGAGGAGAATAT
ACCACCATTGAAATTACATATAAAATCATTCAATGATAGAATAGATAAAGTGGATCAAGA
ATTTGAGAATAAAATTCAACAAATCAGTAAACATAAATTAACCAAACCATCAGCATCCCA
CGATACCGCAATCCTCAAAGACATCCTCTCCATCACAATGCTAGGATCAGGTATTAAAAT
CAATGCTCAACAACAATTAGCACAACAACAACAACAACAAGCTACAATCAATGCATTACA
ACAACAACAAAGATTATTAAATGCACAACAACAACAACAACAGCAACAACAACCACAAGC
ATTA----------ACCNACAGCANCAGTATCANNTGCTGCCACTTATAAACCAGTGGCA
TCACCACAACAAACAAGTTACTTCTAAGCAAGTNCCAATACAGTCAACAAATAAACCATT
ACCACAACAACAAACCTTCAATACAAATTCAACCACAACAATCTATTGTGCAAATGGTGC
AGAATGTTTTACCAAATACAACTTCACCGCCTGTTAACAATAATAATCAATCACCAATTA
ATAGTGGTATTGGAGGAGGTCAATTTAAACCATCAGCTATTAATATTGCAAATGCAAATA
GTGGTACATCAACAATTCAACAACAAATTCAATTACAACAACAACAATTACAACAACAAA
TTCAATTACAACAACAATTAGCACAGCAGCAACAACAAAATCAACAAAATCAACAAAATC
AACAAAACCAGCAAAACCAACAAAATCAACAAATTCATCAACATATTCAACATTTAACAC
CACAACAACAAGCTCAATTACAACAACAAATGTTACAACAACAACAACTTCAACAACAAT
TTCAACAACAACAATTATCACAACAACTTTTACAACAACAACAACTTCATCAACAACAAC
AACCAAATCAAGACAATTCAAAATCCATTTCATAATTTTCAAGCAAATAATTTCAGAGTT
CAAAATCCTACAAATTTTATCCACCACAAAATCAATAAAAGTA
Length of connected seq. 1353
Full length Seq ID -
Full length Seq. -
Length of full length seq. -