>VHN221 (VHN221Q) /CSM/VH/VHN2-A/VHN221Q.Seq.d/
XXXXXXXXXXAAGAAAGTAGTGATACTGAAAAATTAATGGCAAATGAAACTGCTCTCGAG
TATATTATGAATGCTCCAACTCATAGAAGAGATGATCCAATCTTTACCATTCAAGATGGT
TTTGAACCACATGAATTCACTTTTAATTTCCATGCTTGGCAAGTTAATAAAACTCAACAA
GATTCTTATAAATCAAAATTAAGTGCTATCCTTGGTTCAAACAATAGTGGTCCAGCTTCT
CCAATTATGTTACCTACTTCTGGTGTTACTTTAAAACCAACAACAGCAGCTACTCCAAAA
CCAATAACTACACCAACAGTAACTACTCCTAAACCAATAACTACACCGACAGTAGCTACT
CTAAAAACAGTTACACCTGCTGTAACTTTAAAGCCAACAACTGTAACTACTCCATCTAAA
GTTGCTACCACTACAAATACCTCTACTCCATCACCAACTACAATTACAACATTCTATCCA
TTATCAGTTTTAAAACAAAAAACTAATTTACCAAATGATATTGATAAAAGTTGTCTTCAC
TTGTACCTTTCTGATGAAGAATTTTTATCAACCTTTAAAATGACAAAAGAAATCTTCCAA
AAAACTCCTGCTTGGAAAACTAAACAATTACGTGTTNATAATG

---ESSDTEKLMANETALEYIMNAPTHRRDDPIFTIQDGFEPHEFTFNFHAWQVNKTQQD
SYKSKLSAILGSNNSGPASPIMLPTSGVTLKPTTAATPKPITTPTVTTPKPITTPTVATL
KTVTPAVTLKPTTVTTPSKVATTTNTSTPSPTTITTFYPLSVLKQKTNLPNDIDKSCLHL
YLSDEEFLSTFKMTKEIFQKTPAWKTKQLRVXN

Frame A:
---rk**y*kingk*ncsrvyyecsns*kr*snlyhsrwf*tt*ihf*fpclas**nstr
fl*ikikcypwfkq*wssfsnyvtyfwcyfktnnssysktnnytnsnys*tnnytdssys
knsytccnfkanncnysi*scyhykylysitnynynilsiisfktkn*ftk*y**klssl
vpf**rifinl*ndkrnlpknsclen*titcx*


Frame B:
---ESSDTEKLMANETALEYIMNAPTHRRDDPIFTIQDGFEPHEFTFNFHAWQVNKTQQD
SYKSKLSAILGSNNSGPASPIMLPTSGVTLKPTTAATPKPITTPTVTTPKPITTPTVATL
KTVTPAVTLKPTTVTTPSKVATTTNTSTPSPTTITTFYPLSVLKQKTNLPNDIDKSCLHL
YLSDEEFLSTFKMTKEIFQKTPAWKTKQLRVXN


Frame C:
---kvvilkn*wqmkllssil*mlqlieemiqslpfkmvlnhmnsllismlgkliklnki
linqn*vlslvqtivvqllqlcylllvll*nqqqqllqnq*lhqq*lllnq*lhrq*ll*
kqlhll*l*sqql*llhlkllplqipllhhqlqlqhsihyqf*nkkliyqmilikvvftc
tflmknfyqplk*qkksskklllgklnnyvxim

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP894 (VHP894Q) /CSM/VH/VHP8-D/VHP894Q.Seq.d/                       1249   0.0
VHN221 (VHN221Q) /CSM/VH/VHN2-A/VHN221Q.Seq.d/                       1249   0.0
VHK838 (VHK838Q) /CSM/VH/VHK8-B/VHK838Q.Seq.d/                       1249   0.0
VHK614 (VHK614Q) /CSM/VH/VHK6-A/VHK614Q.Seq.d/                       1249   0.0
CHP366 (CHP366Q) /CSM/CH/CHP3-C/CHP366Q.Seq.d/                       1249   0.0
VHA678 (VHA678Q) /CSM/VH/VHA6-D/VHA678Q.Seq.d/                       1243   0.0
VHL689 (VHL689Q) /CSM/VH/VHL6-D/VHL689Q.Seq.d/                       1233   0.0
VHH828 (VHH828Q) /CSM/VH/VHH8-B/VHH828Q.Seq.d/                       1231   0.0
VHO706 (VHO706Q) /CSM/VH/VHO7-A/VHO706Q.Seq.d/                       1223   0.0
VHC852 (VHC852Q) /CSM/VH/VHC8-C/VHC852Q.Seq.d/                       1223   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

X74387|X74387.1 D. discoideum (AX2, AX3) gene for protovillin.   1249   0.0    1
AC114261|AC114261.2 Dictyostelium discoideum chromosome 2 map 126059-159710 strain AX4, complete sequence.  1249   0.0    1
BX842644|BX842644.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 3/4.    38   9e-05  15
AL445563|AL445563.1 Mycoplasma pulmonis (strain UAB CTIP) complete genome; segment 1/3.    40   0.005  12
BX842642|BX842642.1 Mycoplasma mycoides subsp. mycoides SC genomic DNA, complete  sequence; segment 1/4.    32   0.010  11
AE015937|AE015937.1 Clostridium tetani E88, section 2 of 10 of the complete genome.    34   0.038  9
AP004826|AP004826.1 Staphylococcus aureus subsp. aureus MW2 DNA, complete genome, strain:MW2, section 5/10.    36   0.058  7
AC146533|AC146533.2 Otolemur garnettii clone CH256-504C24, WORKING DRAFT SEQUENCE, 3 ordered pieces.    38   0.13   5
AP006581|AP006581.1 Gloeobacter violaceus PCC 7421 DNA, complete genome, section 14/16.    48   0.16   1
AP006628|AP006628.1 Onion yellows phytoplasma OY-M DNA, complete genome.    32   0.24   15

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36418) RecName: Full=Protovillin; AltName: Full=100 kDa actin-...   176   7e-43
FN357417_16(FN357417|pid:none) Schistosoma mansoni genome sequen...    49   2e-04
AY227447_1(AY227447|pid:none) Anableps anableps gelsolin mRNA, c...    47   6e-04
AK154851_1(AK154851|pid:none) Mus musculus NOD-derived CD11c +ve...    45   0.001
BC111730_1(BC111730|pid:none) Homo sapiens advillin, mRNA (cDNA ...    44   0.003
(O75366) RecName: Full=Advillin; AltName: Full=p92;  &AK314362_1...    44   0.003
AF041449_1(AF041449|pid:none) Homo sapiens advillin mRNA, comple...    44   0.003
AY825266_1(AY825266|pid:none) Meleagris gallopavo villin (VIL1) ...    44   0.005
(O65570) RecName: Full=Villin-4;  &AK117296_1(AK117296|pid:none)...    43   0.009
AJ427856_1(AJ427856|pid:none) Dictyostelium discoideum ORF encod...    42   0.020

 psg: 0.75  gvh: 0.33  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.13  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHN221 is nuc

>VHN221Z.Seq
NNNNNNNNNNAAGAAAGTAGTGATACTGAAAAATTAATGGCAAATGAAACTGCTCTCGAG
TATATTATGAATGCTCCAACTCATAGAAGAGATGATCCAATCTTTACCATTCAAGATGGT
TTTGAACCACATGAATTCACTTTTAATTTCCATGCTTGGCAAGTTAATAAAACTCAACAA
GATTCTTATAAATCAAAATTAAGTGCTATCCTTGGTTCAAACAATAGTGGTCCAGCTTCT
CCAATTATGTTACCTACTTCTGGTGTTACTTTAAAACCAACAACAGCAGCTACTCCAAAA
CCAATAACTACACCAACAGTAACTACTCCTAAACCAATAACTACACCGACAGTAGCTACT
CTAAAAACAGTTACACCTGCTGTAACTTTAAAGCCAACAACTGTAACTACTCCATCTAAA
GTTGCTACCACTACAAATACCTCTACTCCATCACCAACTACAATTACAACATTCTATCCA
TTATCAGTTTTAAAACAAAAAACTAATTTACCAAATGATATTGATAAAAGTTGTCTTCAC
TTGTACCTTTCTGATGAAGAATTTTTATCAACCTTTAAAATGACAAAAGAAATCTTCCAA
AAAACTCCTGCTTGGAAAACTAAACAATTACGTGTTNATAATG