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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO639
Library AF
(Link to library)
Clone ID AFO639
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12346-1
Original site URL
Representative seq. ID AFO639P
(Link to Original site)
Representative DNA sequence 
>AFO639 (AFO639Q) /CSM/AF/AFO6-B/AFO639Q.Seq.d/
ATGAGTGTTTTTTACTCCAAATTAAAAATAAATACTACACATAACAAATTATTCAATAAA
TTATAATACAATCATATATTTATATATATATAATAAAATATGAGTACAACCACAGCAACA
CCAACAACAACAACACCAACACCAACAACAACAACACCAACAGATTCACTCTCAAATTAT
AAAGAGGATGAACTTCAAAATCTATCAGTTCAAAAATTAAAAGAATTAATTACCAGTGGG
GGTGGTAGTTATGCAAGTTGCTTTGAAAAAAGTGATTTTGTAGAAAGAGCTAAAACTATA
CGTTCTTCAATGCAAGCATTAGCAAAACCATCCCATGAAACTGTATATAAAGAAAGAATT
GACTTTTATGAATTATTGGGTGTACCATCCACAGCAACAAAGAATGAAATTACAAAAGCA
TATTATAAATTAGCAAAAGAATATCATCCAGATAAAAACAAAAATGATTTATATGCAGAG
GAAATGTTTAAAAAAGTTAGTGAAGCATATTCAGTTTTATCTGATGAAGATAAGAGAAAG
AAATATGATGAATATGGTTTAGATTCAGTTAATGAAATGGATATTGATCCAATTGATTTG
TTTAGAATGATATTTGGTGGTGGATTATTXXXXXXXXXXGCAGCACCAGGTGGTTTGGAT
TTGTTGTCCCTATTGGGTTANATTTACATTCAAGAGGCAAAACAACATTCACTCNTTGGT
TTCTTTCATGAGATCTCTGAGAAAGGTCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
msvfysklkintthnklfnkl*ynhifiyi**NMSTTTATPTTTTPTPTTTTPTDSLSNY
KEDELQNLSVQKLKELITSGGGSYASCFEKSDFVERAKTIRSSMQALAKPSHETVYKERI
DFYELLGVPSTATKNEITKAYYKLAKEYHPDKNKNDLYAEEMFKKVSEAYSVLSDEDKRK
KYDEYGLDSVNEMDIDPIDLFRMIFGGGL---

---AAPGGLDLLSLLGXIYIQEAKQHSLXGFFHEISEKG
Translated Amino Acid sequence (All Frames) 
Frame A:
msvfysklkintthnklfnkl*ynhifiyi**NMSTTTATPTTTTPTPTTTTPTDSLSNY
KEDELQNLSVQKLKELITSGGGSYASCFEKSDFVERAKTIRSSMQALAKPSHETVYKERI
DFYELLGVPSTATKNEITKAYYKLAKEYHPDKNKNDLYAEEMFKKVSEAYSVLSDEDKRK
KYDEYGLDSVNEMDIDPIDLFRMIFGGGL---

---AAPGGLDLLSLLGXIYIQEAKQHSLXGFFHEISEKG

Frame B:
*vfftpn*k*ilhitnysinyntiiylyiynki*vqpqqhqqqqhqhqqqqhqqihsqii
krmnfkiyqfkn*kn*lpvgvvvmqvalkkvil*kelklyvlqckh*qnhpmklyikkel
tfmnywvyhpqqqrmklqkhiin*qkniiqiktkmiymqrkclkklvkhiqfylmkirer
nmmnmv*iqlmkwiliqlicle*ylvvdy---

---qhqvvwiccpywvxftfkrqnnihslvsfmrslrkv

Frame C:
ecfllqiknkyyt*qiiq*iiiqsyiyiyiikyeynhsntnnnntntnnnntnrftlkl*
rg*tsksisskikrinyqwgw*lckll*kk*fcrks*nytffnasisktip*nci*rkn*
ll*iigctihsnke*nyksil*iskrissr*kqk*ficrgnv*ks**sifsfi**r*eke
i**iwfrfs**ngy*sn*fv*ndiwwwii---

---strwfgfvvpiglxlhsrgkttftxwfls*dl*ers
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO639 (AFO639Q) /CSM/AF/AFO6-B/AFO639Q.Seq.d/                       1092   0.0
CFG251 (CFG251Q) /CSM/CF/CFG2-C/CFG251Q.Seq.d/                        926   0.0
CFC472 (CFC472Q) /CSM/CF/CFC4-C/CFC472Q.Seq.d/                        926   0.0
AFN178 (AFN178Q) /CSM/AF/AFN1-D/AFN178Q.Seq.d/                        926   0.0
AFJ269 (AFJ269Q) /CSM/AF/AFJ2-C/AFJ269Q.Seq.d/                        926   0.0
AFE467 (AFE467Q) /CSM/AF/AFE4-C/AFE467Q.Seq.d/                        926   0.0
AFD592 (AFD592Q) /CSM/AF/AFD5-D/AFD592Q.Seq.d/                        918   0.0
AFI503 (AFI503Q) /CSM/AF/AFI5-A/AFI503Q.Seq.d/                        910   0.0
CHC641 (CHC641Q) /CSM/CH/CHC6-B/CHC641Q.Seq.d/                        908   0.0
SFG682 (SFG682Q) /CSM/SF/SFG6-D/SFG682Q.Seq.d/                        906   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BM513627|BM513627.1 kx97b08.y2 Parastrongyloides trichosuri PA SL1 TOPO v1 Murphy Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to WP:CE24911 F22B7.5 ;, mRNA sequence.    48  4e-06  2
AA550161|AA550161.1 1275m3 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 1275m, DNA sequence.    60  4e-05  1
BH157484|BH157484.1 ENTRI87TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ683723|AZ683723.1 ENTHN94TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ545241|AZ545241.1 ENTEI38TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
BH154330|BH154330.1 ENTTQ61TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ681035|AZ681035.1 ENTJN08TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
BH131742|BH131742.1 ENTNB40TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ543842|AZ543842.1 ENTGD17TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
BF296743|BF296743.1 042PbC04 Pb cDNA #17, Tommaso Pace, Marta Ponzi, and Clara Frontali Plasmodium berghei cDNA 5', mRNA sequence.    58  2e-04  1
dna update 2003. 9.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP001098_1266(CP001098|pid:none) Halothermothrix orenii H 168, c...   100   5e-20
BX072555_2(BX072555|pid:none) Zebrafish DNA sequence from clone ...    99   1e-19
BC055555_1(BC055555|pid:none) Danio rerio DnaJ (Hsp40) homolog, ...    99   1e-19
AL845363_2(AL845363|pid:none) Zebrafish DNA sequence from clone ...    99   1e-19
AK001333_1(AK001333|pid:none) Homo sapiens cDNA FLJ10471 fis, cl...    98   3e-19
AY890460_1(AY890460|pid:none) Synthetic construct Homo sapiens c...    97   4e-19
BC161074_1(BC161074|pid:none) Xenopus tropicalis hypothetical pr...    97   4e-19
AF411044_1(AF411044|pid:none) Homo sapiens DnaJ protein Tid-1 mR...    97   4e-19
AF061749_1(AF061749|pid:none) Homo sapiens tumorous imaginal dis...    97   4e-19
AF325535_1(AF325535|pid:none) Mus musculus tumorous imaginal dis...    97   5e-19
protein update 2009. 4.26
PSORT 

 psg: 0.86  gvh: 0.51  alm: 0.39  top: 0.27  tms: 0.00  mit: 0.44  mip: 0.00
 nuc: 0.10  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 24.0 %: extracellular, including cell wall
	 24.0 %: nuclear
	 20.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for AFO639 is exc
5' end seq. ID AFO639F
5' end seq. 
>AFO639F.Seq
ATGAGTGTTTTTTACTCCAAATTAAAAATAAATACTACACATAACAAATTATTCAATAAA
TTATAATACAATCATATATTTATATATATATAATAAAATATGAGTACAACCACAGCAACA
CCAACAACAACAACACCAACACCAACAACAACAACACCAACAGATTCACTCTCAAATTAT
AAAGAGGATGAACTTCAAAATCTATCAGTTCAAAAATTAAAAGAATTAATTACCAGTGGG
GGTGGTAGTTATGCAAGTTGCTTTGAAAAAAGTGATTTTGTAGAAAGAGCTAAAACTATA
CGTTCTTCAATGCAAGCATTAGCAAAACCATCCCATGAAACTGTATATAAAGAAAGAATT
GACTTTTATGAATTATTGGGTGTACCATCCACAGCAACAAAGAATGAAATTACAAAAGCA
TATTATAAATTAGCAAAAGAATATCATCCAGATAAAAACAAAAATGATTTATATGCAGAG
GAAATGTTTAAAAAAGTTAGTGAAGCATATTCAGTTTTATCTGATGAAGATAAGAGAAAG
AAATATGATGAATATGGTTTAGATTCAGTTAATGAAATGGATATTGATCCAATTGATTTG
TTTAGAATGATATTTGGTGGTGGATTATT----------
Length of 5' end seq. 629
3' end seq. ID AFO639Z
3' end seq. 
>AFO639Z.Seq
----------GCAGCACCAGGTGGTTTGGATTTGTTGTCCCTATTGGGTTANATTTACAT
TCAAGAGGCAAAACAACATTCACTCNTTGGTTTCTTTCATGAGATCTCTGAGAAAGGTCA
Length of 3' end seq. 110
Connected seq. ID AFO639P
Connected seq. 
>AFO639P.Seq
ATGAGTGTTTTTTACTCCAAATTAAAAATAAATACTACACATAACAAATTATTCAATAAA
TTATAATACAATCATATATTTATATATATATAATAAAATATGAGTACAACCACAGCAACA
CCAACAACAACAACACCAACACCAACAACAACAACACCAACAGATTCACTCTCAAATTAT
AAAGAGGATGAACTTCAAAATCTATCAGTTCAAAAATTAAAAGAATTAATTACCAGTGGG
GGTGGTAGTTATGCAAGTTGCTTTGAAAAAAGTGATTTTGTAGAAAGAGCTAAAACTATA
CGTTCTTCAATGCAAGCATTAGCAAAACCATCCCATGAAACTGTATATAAAGAAAGAATT
GACTTTTATGAATTATTGGGTGTACCATCCACAGCAACAAAGAATGAAATTACAAAAGCA
TATTATAAATTAGCAAAAGAATATCATCCAGATAAAAACAAAAATGATTTATATGCAGAG
GAAATGTTTAAAAAAGTTAGTGAAGCATATTCAGTTTTATCTGATGAAGATAAGAGAAAG
AAATATGATGAATATGGTTTAGATTCAGTTAATGAAATGGATATTGATCCAATTGATTTG
TTTAGAATGATATTTGGTGGTGGATTATT----------GCAGCACCAGGTGGTTTGGAT
TTGTTGTCCCTATTGGGTTANATTTACATTCAAGAGGCAAAACAACATTCACTCNTTGGT
TTCTTTCATGAGATCTCTGAGAAAGGTCA
Length of connected seq. 739
Full length Seq ID -
Full length Seq. -
Length of full length seq. -