>AFO620 (AFO620Q) /CSM/AF/AFO6-A/AFO620Q.Seq.d/
ATGATAAAGCTGTTCATTATTTTTTCATTTTTTTTTTTATTTTTTAGNGNCATAATTCAT
CAAGCAAAACAACCACAACANGAACAACAACAACAAATGTCAATTTTAAAAAGTTTTATT
GAAGTTAGNGAAGATTCACACTTTCCAATTCAAAATCTTCCATATGGNGTCTTTAAACCA
ACCCTAAATGATCAAGCTAGAATTGGNGTTGCTATTGGNGATTTCGTTTGNGATTTATCA
GTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATACTAAAGTTTTTCATCAA
GAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGAGNGAAGCTAGAAAAACC
ATTCAAAATTTATTATCATCAGAAACATCAACTNTTAGAGATAATAAAGAGTNTAGAGAA
AAGATNTTTCATTCAATTTCAAGNGTAACAATGTTATTACCAGCAAGAATTGGAGATTAT
ACTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTNTTATGTTTAGAGGTAAA
GAGAATGCATTGATGCCAAATTGGGTAXXXXXXXXXXCATTACCAACTTTTAATCCATGT
AGATTATTGGATTTTGAATTGGAGATGGGCGCTTTGATTGGAGGAGAATCAACCAAATTG
GGTGAACCAATTTCAATTGAGTCTGCAAAAGATCATATCTTTGGTTTGGTACTTTTGAAT
GATTGGAGTGCAAGAGATATCCAAAAATGGGAATACGTTCCATTGGGTCCATTCTTGGCA
AAGAATTTCGGCTCAACTATATCACCATGGGTTGTTACAATGGAGGCATTGCAACCATTT
GCTACAAAACCACCAACTCAAGATCCACAACCAATGAAATATCTTCAAGAACAAGGTAAT
ACTACCTTTGATATTGAATTATCAGTTAGTATTAAATCTCCAAAAATGTCAAAACCTCAT
AAGGTTTCAACTTCAAATTTGAAATACATGTATTGGACACTCACTCAACAATTGGCTCAT
CATACTGTAAATGGTTGTAATATGAATGCTGGTGATTTACTTGGTACTGGTACAATTAGT
GGTCCAACTGAAGATAGCTATGGTTCAATGTTGGAACTCTCTTGGAAAGGTAGTAAAGTG
GTCTCATTAGGTACTGAAACAAATGAAGAAAGAAAATTCATTCAAGATGGTGATTCTGTT
ATCTTATCNGGTCTTTGTAAAGGTAATGGTTATCAAATTGGTTTGGAATTGGAAGGTACA
ATTTACCAGC

MIKLFIIFSFFFLFFXXIIHQAKQPQXEQQQQMSILKSFIEVXEDSHFPIQNLPYGVFKP
TLNDQARIGVAIGDFVXDLSVLADLKLFDGKLKDTKVFHQENLNSFMSLGKELWXEARKT
IQNLLSSETSTXRDNKEXREKXFHSISXVTMLLPARIGDYTDFYASKEHATNVGXMFRGK
ENALMPNWV---

---LPTFNPCRLLDFELEMGALIGGESTKLGEPISIESAKDHIFGLVLLNDWSARDIQKW
EYVPLGPFLAKNFGSTISPWVVTMEALQPFATKPPTQDPQPMKYLQEQGNTTFDIELSVS
IKSPKMSKPHKVSTSNLKYMYWTLTQQLAHHTVNGCNMNAGDLLGTGTISGPTEDSYGSM
LELSWKGSKVVSLGTETNEERKFIQDGDSVILSGLCKGNGYQIGLELEGTIYQ

Frame A:
MIKLFIIFSFFFLFFXXIIHQAKQPQXEQQQQMSILKSFIEVXEDSHFPIQNLPYGVFKP
TLNDQARIGVAIGDFVXDLSVLADLKLFDGKLKDTKVFHQENLNSFMSLGKELWXEARKT
IQNLLSSETSTXRDNKEXREKXFHSISXVTMLLPARIGDYTDFYASKEHATNVGXMFRGK
ENALMPNWV---

---hyqllihvdywilnwrwal*leenqpnwvnqfqlslqkiislvwyf*migvqeiskn
gntfhwvhswqrisaqlyhhgllqwrhcnhllqnhqlkihnq*nifknkvilplilnyql
vlnlqkcqnlirfqlqi*ntcighslnnwliil*mvvi*mlviylvlvqlvvqlkiamvq
cwnslgkvvkwsh*vlkqmkkensfkmvillsyxvfvkvmviklvwnwkvqfts

Frame B:
**scslffhfffyflxx*fikqnnhnxnnnnkcqf*kvllklxkihtfqfkifhmxslnq
p*miklelxlllxisfxiyqf*qi*nflmvn*kilkffikki*ihlch*vknygxklekp
fkiyyhqkhqlleiiksxekrxfiqfqx*qcyyqqeleiilismhqknmqpmlvlclevk
rmh*cqig---

---itnf*sm*iigf*igdgrfdwrrinqig*tnfn*vckrsylwfgtfe*leckrypkm
girsigsilgkefrlnyitmgcynggiaticykttnsrsttneissrtr*yyl*y*iis*
y*isknvkts*gfnfkfeihvldthstigssyckwl*yecw*ftwywyn*wsn*r*lwfn
vgtller**sgliry*nk*rkkihsrw*fcylixsl*r*wlsnwfgigrynlp

Frame C:
dkavhyffifffif*xhnssskttttxttttnvnfkkfy*s*xrftlsnskssiwxl*tn
pk*ss*nwxcywxfrlxfisfsrfktf*w*ikry*sfssrkfkfiyvir*rimexs*knh
skfiiirninx*r**rv*rkdxsfnfkxnnvitsknwrly*flcikrtcnqcwxyv*r*r
ecidaklg---

---LPTFNPCRLLDFELEMGALIGGESTKLGEPISIESAKDHIFGLVLLNDWSARDIQKW
EYVPLGPFLAKNFGSTISPWVVTMEALQPFATKPPTQDPQPMKYLQEQGNTTFDIELSVS
IKSPKMSKPHKVSTSNLKYMYWTLTQQLAHHTVNGCNMNAGDLLGTGTISGPTEDSYGSM
LELSWKGSKVVSLGTETNEERKFIQDGDSVILSGLCKGNGYQIGLELEGTIYQ

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO620 (AFO620Q) /CSM/AF/AFO6-A/AFO620Q.Seq.d/                       2196   0.0
SFD819 (SFD819Q) /CSM/SF/SFD8-A/SFD819Q.Seq.d/                       1316   0.0
CFA612 (CFA612Q) /CSM/CF/CFA6-A/CFA612Q.Seq.d/                       1300   0.0
AFK792 (AFK792Q) /CSM/AF/AFK7-D/AFK792Q.Seq.d/                       1289   0.0
AFN775 (AFN775Q) /CSM/AF/AFN7-D/AFN775Q.Seq.d/                       1285   0.0
AFE706 (AFE706Q) /CSM/AF/AFE7-A/AFE706Q.Seq.d/                       1283   0.0
AFO449 (AFO449Q) /CSM/AF/AFO4-C/AFO449Q.Seq.d/                       1281   0.0
AFJ754 (AFJ754Q) /CSM/AF/AFJ7-C/AFJ754Q.Seq.d/                       1273   0.0
CHD537 (CHD537Q) /CSM/CH/CHD5-B/CHD537Q.Seq.d/                       1253   0.0
SFF638 (SFF638Q) /CSM/SF/SFF6-B/SFF638Q.Seq.d/                       1231   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BM774584|BM774584.1 ra95c11.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to TR:Q94272 Q94272 SIMILAR TO FUMARYLACETOACETASE. [1] ;, mRNA sequence.    64  1e-18  3
BI504681|BI504681.1 BB170011A20F01 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170011A20F01 5', mRNA sequence.    64  2e-10  3
AW827814|AW827814.1 ra59b10.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to WP:K10C2.4 CE04750 FUMARYLACETOACETASE ;, mRNA sequence.    48  4e-07  3
AW871572|AW871572.1 ra79e06.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to WP:K10C2.4 CE04750 FUMARYLACETOACETASE ;, mRNA sequence.    48  5e-07  3
BQ475268|BQ475268.1 cicindela4h01.b Cicindela campestris cDNA Cicindela campestris cDNA clone cicindela4h01 5', mRNA sequence.    50  3e-06  2
CF440839|CF440839.1 EST677184 normalized cDNA library of onion Allium cepa cDNA clone ACAFM72, mRNA sequence.    64  5e-06  1
CF446374|CF446374.1 EST682719 normalized cDNA library of onion Allium cepa cDNA clone ACAHY76, mRNA sequence.    64  5e-06  1
BI933163|BI933163.1 EST553052 tomato flower, 8 mm to preanthesis buds Lycopersicon esculentum cDNA clone cTOC25I18 5' end, mRNA sequence.    56  1e-05  2
BI422229|BI422229.1 EST532895 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC69O22 5' end, mRNA sequence.    56  1e-05  2
BM410976|BM410976.1 EST585303 tomato breaker fruit Lycopersicon esculentum cDNA clone cLEG55C6 5' end, mRNA sequence.    56  1e-05  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXQ1) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   735   0.0
(A5PKH3) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   432   e-119
EF144286_1(EF144286|pid:none) Populus trichocarpa clone PX0015_F...   430   e-119
AK143759_1(AK143759|pid:none) Mus musculus 6 days neonate spleen...   426   e-118
AY891819_1(AY891819|pid:none) Synthetic construct Homo sapiens c...   424   e-117
(P16930) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   424   e-117
A56825(A56825;S21387) fumarylacetoacetase (EC 3.7.1.2) - mouse  ...   423   e-117
(P25093) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   417   e-115
T25813(T25813) hypothetical protein K10C2.4 - Caenorhabditis ele...   416   e-115
BC157696_1(BC157696|pid:none) Xenopus tropicalis hypothetical pr...   412   e-113

 psg: 1.02  gvh: 0.55  alm: 0.23  top: 0.43  tms: 0.07  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for AFO620 is end

>AFO620F.Seq
ATGATAAAGCTGTTCATTATTTTTTCATTTTTTTTTTTATTTTTTAGNGNCATAATTCAT
CAAGCAAAACAACCACAACANGAACAACAACAACAAATGTCAATTTTAAAAAGTTTTATT
GAAGTTAGNGAAGATTCACACTTTCCAATTCAAAATCTTCCATATGGNGTCTTTAAACCA
ACCCTAAATGATCAAGCTAGAATTGGNGTTGCTATTGGNGATTTCGTTTGNGATTTATCA
GTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATACTAAAGTTTTTCATCAA
GAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGAGNGAAGCTAGAAAAACC
ATTCAAAATTTATTATCATCAGAAACATCAACTNTTAGAGATAATAAAGAGTNTAGAGAA
AAGATNTTTCATTCAATTTCAAGNGTAACAATGTTATTACCAGCAAGAATTGGAGATTAT
ACTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTNTTATGTTTAGAGGTAAA
GAGAATGCATTGATGCCAAATTGGGTA----------

>AFO620Z.Seq
----------CATTACCAACTTTTAATCCATGTAGATTATTGGATTTTGAATTGGAGATG
GGCGCTTTGATTGGAGGAGAATCAACCAAATTGGGTGAACCAATTTCAATTGAGTCTGCA
AAAGATCATATCTTTGGTTTGGTACTTTTGAATGATTGGAGTGCAAGAGATATCCAAAAA
TGGGAATACGTTCCATTGGGTCCATTCTTGGCAAAGAATTTCGGCTCAACTATATCACCA
TGGGTTGTTACAATGGAGGCATTGCAACCATTTGCTACAAAACCACCAACTCAAGATCCA
CAACCAATGAAATATCTTCAAGAACAAGGTAATACTACCTTTGATATTGAATTATCAGTT
AGTATTAAATCTCCAAAAATGTCAAAACCTCATAAGGTTTCAACTTCAAATTTGAAATAC
ATGTATTGGACACTCACTCAACAATTGGCTCATCATACTGTAAATGGTTGTAATATGAAT
GCTGGTGATTTACTTGGTACTGGTACAATTAGTGGTCCAACTGAAGATAGCTATGGTTCA
ATGTTGGAACTCTCTTGGAAAGGTAGTAAAGTGGTCTCATTAGGTACTGAAACAAATGAA
GAAAGAAAATTCATTCAAGATGGTGATTCTGTTATCTTATCNGGTCTTTGTAAAGGTAAT
GGTTATCAAATTGGTTTGGAATTGGAAGGTACAATTTACCAGC

>AFO620P.Seq
ATGATAAAGCTGTTCATTATTTTTTCATTTTTTTTTTTATTTTTTAGNGNCATAATTCAT
CAAGCAAAACAACCACAACANGAACAACAACAACAAATGTCAATTTTAAAAAGTTTTATT
GAAGTTAGNGAAGATTCACACTTTCCAATTCAAAATCTTCCATATGGNGTCTTTAAACCA
ACCCTAAATGATCAAGCTAGAATTGGNGTTGCTATTGGNGATTTCGTTTGNGATTTATCA
GTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATACTAAAGTTTTTCATCAA
GAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGAGNGAAGCTAGAAAAACC
ATTCAAAATTTATTATCATCAGAAACATCAACTNTTAGAGATAATAAAGAGTNTAGAGAA
AAGATNTTTCATTCAATTTCAAGNGTAACAATGTTATTACCAGCAAGAATTGGAGATTAT
ACTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTNTTATGTTTAGAGGTAAA
GAGAATGCATTGATGCCAAATTGGGTA----------CATTACCAACTTTTAATCCATGT
AGATTATTGGATTTTGAATTGGAGATGGGCGCTTTGATTGGAGGAGAATCAACCAAATTG
GGTGAACCAATTTCAATTGAGTCTGCAAAAGATCATATCTTTGGTTTGGTACTTTTGAAT
GATTGGAGTGCAAGAGATATCCAAAAATGGGAATACGTTCCATTGGGTCCATTCTTGGCA
AAGAATTTCGGCTCAACTATATCACCATGGGTTGTTACAATGGAGGCATTGCAACCATTT
GCTACAAAACCACCAACTCAAGATCCACAACCAATGAAATATCTTCAAGAACAAGGTAAT
ACTACCTTTGATATTGAATTATCAGTTAGTATTAAATCTCCAAAAATGTCAAAACCTCAT
AAGGTTTCAACTTCAAATTTGAAATACATGTATTGGACACTCACTCAACAATTGGCTCAT
CATACTGTAAATGGTTGTAATATGAATGCTGGTGATTTACTTGGTACTGGTACAATTAGT
GGTCCAACTGAAGATAGCTATGGTTCAATGTTGGAACTCTCTTGGAAAGGTAGTAAAGTG
GTCTCATTAGGTACTGAAACAAATGAAGAAAGAAAATTCATTCAAGATGGTGATTCTGTT
ATCTTATCNGGTCTTTGTAAAGGTAATGGTTATCAAATTGGTTTGGAATTGGAAGGTACA
ATTTACCAGC