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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB721
Library AF
(Link to library)
Clone ID AFB721
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16392-1
Original site URL
Representative seq. ID AFB721P
(Link to Original site)
Representative DNA sequence 
>AFB721 (AFB721Q) /CSM/AF/AFB7-A/AFB721Q.Seq.d/
ATTTAATAATACACATTCTTTCCACCAATCATTTTTTATATAAATTTTTATAAAAACGTT
TTTTAATTTAATTGTTGCGTTTAAACAATGAGGTTAGGATCCATATCATCTCTATCTTGT
GATCAACTGTGTAAATATTTACATTCATCACTTGGTGATGAATATTCAAACATCATTAAT
AGTTTTAAAATAAATCAAGTCACTGGTGATGATTTTGTTTTATTTGAACAAAAAGATTAT
GAAACTTTATCAGTACCTTGGAAATTTCAAAAAAAACTTAAAGAAATACAAGGTTCTGGT
GTTGTAGATAATCCAGGCTCAACTCCAAACCCAAGCCCAAACCCAAGTCCAAACCCAGTA
CCTGGCAATACTTCAAATGCACCAACATCAACAGGTAGAGAATCACTTGAGACCCAAAGA
GATATCATACAAATGAAAAGTAAAATTGATCTTCTCAGTAATTCATTGTTACAACTAGTT
GAGCAAATGAAAGTTACCAAACCACCTACAAATAACTTTTCAAATTTATCACCAGCACCA
CAAAATAAAAATATTGAATCCATCACCTCAATCAAXXXXXXXXXXATTAAAACACCTCTT
AGTGAACCTATAGATGGTACTATTGAATTGATGTATGGTGGTAGAAATATGTTTACTGGC
AGTGTTACACAAGGTGATGCTGATCTTCCTCTAAACCTCTTGGATGGTACATTTGAGGTT
ATCATTAAACCCAATAAGAGTAATTTATTACCTTTAAAGTTTAAAATGATTATACATGAC
TCTGTAAGAACCACTTCAAGCCATCAAAATGTCGGTAGACCTAAAAAGCCTAAAGAAATG
GATTTCGTTTTAGCTTGGGGGGAATCACCACTGGATTTGGATTCTCATATGTGGTGTATT
CATCCTAATGGTTCTATTAGTCATGTTTATTTCGGGGGTAGGAGTGTAGATAAAATGTCC
TTGGAAGCTGATACTCAAAATGGTTTTGGTCCCGAAACCATAAGTATTATACGTTTAGAT
GGTTGTAAATATGTTTATGCAGTTCATAATTTCTCAACTGAAGGAGAAATAGCTCAATCA
GGTGCCAAGCTTACAATTGCCCATGAAACCTCAACATTATTGAAAGATGTATCCCAAACA
CTCGACAAACTGGTGCAAGATTTTGGGTAGTATGTACTATTGATGGAACATCTGGAGATA
TGACTTCAAAGATCATTTNAATTCCACAATTCACAAACAGAATTCCATTA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
i**ytffppiifyinfyknvf*fnccv*TMRLGSISSLSCDQLCKYLHSSLGDEYSNIIN
SFKINQVTGDDFVLFEQKDYETLSVPWKFQKKLKEIQGSGVVDNPGSTPNPSPNPSPNPV
PGNTSNAPTSTGRESLETQRDIIQMKSKIDLLSNSLLQLVEQMKVTKPPTNNFSNLSPAP
QNKNIESITSI---

---IKTPLSEPIDGTIELMYGGRNMFTGSVTQGDADLPLNLLDGTFEVIIKPNKSNLLPL
KFKMIIHDSVRTTSSHQNVGRPKKPKEMDFVLAWGESPLDLDSHMWCIHPNGSISHVYFG
GRSVDKMSLEADTQNGFGPETISIIRLDGCKYVYAVHNFSTEGEIAQSGAKLTIAHETST
LLKDVSQTLDKLVQDFG*yvllmehlei*lqrsfxfhnsqtefh
Translated Amino Acid sequence (All Frames) 
Frame A:
i**ytffppiifyinfyknvf*fnccv*TMRLGSISSLSCDQLCKYLHSSLGDEYSNIIN
SFKINQVTGDDFVLFEQKDYETLSVPWKFQKKLKEIQGSGVVDNPGSTPNPSPNPSPNPV
PGNTSNAPTSTGRESLETQRDIIQMKSKIDLLSNSLLQLVEQMKVTKPPTNNFSNLSPAP
QNKNIESITSI---

---IKTPLSEPIDGTIELMYGGRNMFTGSVTQGDADLPLNLLDGTFEVIIKPNKSNLLPL
KFKMIIHDSVRTTSSHQNVGRPKKPKEMDFVLAWGESPLDLDSHMWCIHPNGSISHVYFG
GRSVDKMSLEADTQNGFGPETISIIRLDGCKYVYAVHNFSTEGEIAQSGAKLTIAHETST
LLKDVSQTLDKLVQDFG*yvllmehlei*lqrsfxfhnsqtefh

Frame B:
fnnthsfhqsffi*ifiktffnlivafkq*g*dpyhlylvincvniyihhlvmniqtsli
vlk*ikslvmilfylnkkimklyqylgnfkknlkkykvlvl*iiqaqlqtqaqtqvqtqy
lailqmhqhqqvenhlrpkeisyk*kvklifsvihcyn*lsk*klpnhlqitfqiyhqhh
kikilnpspqs---

---lkhllvnl*mvlln*cmvveicllavlhkvmlifl*tswmvhlrlslnpirviyyl*
slk*lymtl*eplqaikmsvdlkslkkwisf*lggnhhwiwilicgvfilmvllvmfisg
vgv*ikcpwklilkmvlvpkp*vlyv*mvvnmfmqfiisqlkek*lnqvpslqlpmkpqh
y*kmypkhstnwckilgsmyy*wniwrydfkdhxnstihkqnsi

Frame C:
liihilstnhflykfl*krfli*llrlnnevrihiisil*stv*iftfitw**ifkhh**
f*nksshw**fcfi*tkrl*nfistleiskkt*rntrfwccr*srlnskpkpkpkskpst
wqyfkctninr*rit*dpkryhtnek*n*ssq*fivtts*anesyqttyk*lfkfitstt
k*ky*ihhlnq---

---*nts**tyrwyy*idvww*kyvywqcytr*c*ssskplgwyi*gyh*tq*e*fitfk
v*ndyt*lcknhfkpskcr*t*ka*rngfrfslggittgfgfsyvvyss*wfy*sclfrg
*ecr*nvlgs*yskwfwsrnhkyytfrwl*iclcss*fln*rrnssircqayncp*nlni
iercipntrqtgarfwvvctidgtsgdmtskiixipqftnripl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB721 (AFB721Q) /CSM/AF/AFB7-A/AFB721Q.Seq.d/                       2228   0.0
AFK163 (AFK163Q) /CSM/AF/AFK1-C/AFK163Q.Seq.d/                       1296   0.0
AFD590 (AFD590Q) /CSM/AF/AFD5-D/AFD590Q.Seq.d/                       1294   0.0
AFJ140 (AFJ140Q) /CSM/AF/AFJ1-B/AFJ140Q.Seq.d/                       1292   0.0
AFI545 (AFI545Q) /CSM/AF/AFI5-B/AFI545Q.Seq.d/                       1291   0.0
AFD183 (AFD183Q) /CSM/AF/AFD1-D/AFD183Q.Seq.d/                       1285   0.0
AFE636 (AFE636Q) /CSM/AF/AFE6-B/AFE636Q.Seq.d/                       1281   0.0
AFD860 (AFD860Q) /CSM/AF/AFD8-C/AFD860Q.Seq.d/                       1281   0.0
CFA294 (CFA294Q) /CSM/CF/CFA2-D/CFA294Q.Seq.d/                       1275   0.0
CFH243 (CFH243Q) /CSM/CF/CFH2-B/CFH243Q.Seq.d/                       1271   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC005421|AC005421.1 Drosophila melanogaster, chromosome 2L, region 22A1-22A1, P1 clone DS03601, complete sequence.    56  0.001  1
AC017558|AC017558.1 Drosophila melanogaster, *** SEQUENCING IN PROGRESS ***, in ordered pieces.    56  0.001  1
AC009741|AC009741.5 Drosophila melanogaster, chromosome 3R, region 87B-87B, BAC clone BACR44K17, complete sequence.    44  0.007  2
AC135693|AC135693.2 Rattus norvegicus clone CH230-244M15, WORKING DRAFT SEQUENCE, 11 unordered pieces.    58  0.009  3
AC103457|AC103457.6 Rattus norvegicus clone CH230-42P7, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    58  0.012  2
BH721542|BH721542.1 BOMGK60TR BO_2_3_KB Brassica oleracea genomic clone BOMGK60, DNA sequence.    50  0.067  1
BZ465810|BZ465810.1 BONOE77TF BO_1.6_2_KB_tot Brassica oleracea genomic clone BONOE77, DNA sequence.    50  0.067  1
BH420909|BH420909.1 BOGPL92TR BOGP Brassica oleracea genomic clone BOGPL92, DNA sequence.    50  0.067  1
AC102995|AC102995.7 Rattus norvegicus clone CH230-67N20, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    58  0.10  3
AE003586|AE003586.3 Drosophila melanogaster chromosome 2L, section 5 of 83 of the complete sequence.    56  0.14  4
dna update 2003. 7.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000880_2513(CP000880|pid:none) Salmonella enterica subsp. ariz...    66   2e-09
CP000478_3794(CP000478|pid:none) Syntrophobacter fumaroxidans MP...    61   4e-08
CU928162_679(CU928162|pid:none) Escherichia coli ED1a chromosome...    62   4e-08
AE014075_780(AE014075|pid:none) Escherichia coli CFT073, complet...    60   2e-07
CP000243_708(CP000243|pid:none) Escherichia coli UTI89, complete...    60   2e-07
CP000112_1873(CP000112|pid:none) Desulfovibrio desulfuricans G20...    59   3e-07
AE001437_1067(AE001437|pid:none) Clostridium acetobutylicum ATCC...    58   6e-07
CP000247_733(CP000247|pid:none) Escherichia coli 536, complete g...    58   6e-07
FM954973_832(FM954973|pid:none) Vibrio splendidus LGP32 chromoso...    56   2e-06
AE001437_1068(AE001437|pid:none) Clostridium acetobutylicum ATCC...    55   5e-06
protein update 2009. 3.27
PSORT 

 psg: 0.88  gvh: 0.44  alm: 0.48  top: 0.30  tms: 0.00  mit: 0.28  mip: 0.02
 nuc: 0.12  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for AFB721 is cyt
5' end seq. ID AFB721F
5' end seq. 
>AFB721F.Seq
ATTTAATAATACACATTCTTTCCACCAATCATTTTTTATATAAATTTTTATAAAAACGTT
TTTTAATTTAATTGTTGCGTTTAAACAATGAGGTTAGGATCCATATCATCTCTATCTTGT
GATCAACTGTGTAAATATTTACATTCATCACTTGGTGATGAATATTCAAACATCATTAAT
AGTTTTAAAATAAATCAAGTCACTGGTGATGATTTTGTTTTATTTGAACAAAAAGATTAT
GAAACTTTATCAGTACCTTGGAAATTTCAAAAAAAACTTAAAGAAATACAAGGTTCTGGT
GTTGTAGATAATCCAGGCTCAACTCCAAACCCAAGCCCAAACCCAAGTCCAAACCCAGTA
CCTGGCAATACTTCAAATGCACCAACATCAACAGGTAGAGAATCACTTGAGACCCAAAGA
GATATCATACAAATGAAAAGTAAAATTGATCTTCTCAGTAATTCATTGTTACAACTAGTT
GAGCAAATGAAAGTTACCAAACCACCTACAAATAACTTTTCAAATTTATCACCAGCACCA
CAAAATAAAAATATTGAATCCATCACCTCAATCAA----------
Length of 5' end seq. 575
3' end seq. ID AFB721Z
3' end seq. 
>AFB721Z.Seq
----------ATTAAAACACCTCTTAGTGAACCTATAGATGGTACTATTGAATTGATGTA
TGGTGGTAGAAATATGTTTACTGGCAGTGTTACACAAGGTGATGCTGATCTTCCTCTAAA
CCTCTTGGATGGTACATTTGAGGTTATCATTAAACCCAATAAGAGTAATTTATTACCTTT
AAAGTTTAAAATGATTATACATGACTCTGTAAGAACCACTTCAAGCCATCAAAATGTCGG
TAGACCTAAAAAGCCTAAAGAAATGGATTTCGTTTTAGCTTGGGGGGAATCACCACTGGA
TTTGGATTCTCATATGTGGTGTATTCATCCTAATGGTTCTATTAGTCATGTTTATTTCGG
GGGTAGGAGTGTAGATAAAATGTCCTTGGAAGCTGATACTCAAAATGGTTTTGGTCCCGA
AACCATAAGTATTATACGTTTAGATGGTTGTAAATATGTTTATGCAGTTCATAATTTCTC
AACTGAAGGAGAAATAGCTCAATCAGGTGCCAAGCTTACAATTGCCCATGAAACCTCAAC
ATTATTGAAAGATGTATCCCAAACACTCGACAAACTGGTGCAAGATTTTGGGTAGTATGT
ACTATTGATGGAACATCTGGAGATATGACTTCAAAGATCATTTNAATTCCACAATTCACA
AACAGAATTCCATTA
Length of 3' end seq. 665
Connected seq. ID AFB721P
Connected seq. 
>AFB721P.Seq
ATTTAATAATACACATTCTTTCCACCAATCATTTTTTATATAAATTTTTATAAAAACGTT
TTTTAATTTAATTGTTGCGTTTAAACAATGAGGTTAGGATCCATATCATCTCTATCTTGT
GATCAACTGTGTAAATATTTACATTCATCACTTGGTGATGAATATTCAAACATCATTAAT
AGTTTTAAAATAAATCAAGTCACTGGTGATGATTTTGTTTTATTTGAACAAAAAGATTAT
GAAACTTTATCAGTACCTTGGAAATTTCAAAAAAAACTTAAAGAAATACAAGGTTCTGGT
GTTGTAGATAATCCAGGCTCAACTCCAAACCCAAGCCCAAACCCAAGTCCAAACCCAGTA
CCTGGCAATACTTCAAATGCACCAACATCAACAGGTAGAGAATCACTTGAGACCCAAAGA
GATATCATACAAATGAAAAGTAAAATTGATCTTCTCAGTAATTCATTGTTACAACTAGTT
GAGCAAATGAAAGTTACCAAACCACCTACAAATAACTTTTCAAATTTATCACCAGCACCA
CAAAATAAAAATATTGAATCCATCACCTCAATCAA----------ATTAAAACACCTCTT
AGTGAACCTATAGATGGTACTATTGAATTGATGTATGGTGGTAGAAATATGTTTACTGGC
AGTGTTACACAAGGTGATGCTGATCTTCCTCTAAACCTCTTGGATGGTACATTTGAGGTT
ATCATTAAACCCAATAAGAGTAATTTATTACCTTTAAAGTTTAAAATGATTATACATGAC
TCTGTAAGAACCACTTCAAGCCATCAAAATGTCGGTAGACCTAAAAAGCCTAAAGAAATG
GATTTCGTTTTAGCTTGGGGGGAATCACCACTGGATTTGGATTCTCATATGTGGTGTATT
CATCCTAATGGTTCTATTAGTCATGTTTATTTCGGGGGTAGGAGTGTAGATAAAATGTCC
TTGGAAGCTGATACTCAAAATGGTTTTGGTCCCGAAACCATAAGTATTATACGTTTAGAT
GGTTGTAAATATGTTTATGCAGTTCATAATTTCTCAACTGAAGGAGAAATAGCTCAATCA
GGTGCCAAGCTTACAATTGCCCATGAAACCTCAACATTATTGAAAGATGTATCCCAAACA
CTCGACAAACTGGTGCAAGATTTTGGGTAGTATGTACTATTGATGGAACATCTGGAGATA
TGACTTCAAAGATCATTTNAATTCCACAATTCACAAACAGAATTCCATTA
Length of connected seq. 1240
Full length Seq ID -
Full length Seq. -
Length of full length seq. -