>VHM627 (VHM627Q) /CSM/VH/VHM6-B/VHM627Q.Seq.d/
XXXXXXXXXXTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTAT
ATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAGAATTCGTACC
AGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGA
AACAATTCAAGGGTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTAT
CAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGNTACAGAGTTTCGAAATA
TCGCTAATCAACATNTAAAGAATTTNTATATCATCGTACCTGCCTTGGCCTCAAATTACA
TCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTG
AAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTAT
TAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTG
AAACNGATCAAAAGAGAATGT

---rsf*tfnhsyw*cygly*igqirwftll**cyqnsyqt*krfqnski*PQKMPSHLK
QFKGSTNLDSIIHNLSNNLSEGTEYFKMLVNVFXTEFRNIANQHXKNXYIIVPALASNYI
DHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCE
TDQKRM

Frame A:
---rsf*tfnhsyw*cygly*igqirwftll**cyqnsyqt*krfqnski*PQKMPSHLK
QFKGSTNLDSIIHNLSNNLSEGTEYFKMLVNVFXTEFRNIANQHXKNXYIIVPALASNYI
DHMINSKDKLFKKSKAIGAEALLFTDDGFAIGLAYILKLLDQNKDFDSLHWFDRIQLKCE
TDQKRM


Frame B:
---dhfrllithignamgyirlvrsgglhycsnairirtrlekdskiprfnlkrcplt*n
nsrvvpiwiqsfiifqiiyqrvlntlrcw*tfsxqsfeislinx*rixissylpwpqits
ii*sivkinslrnqkqsvlklyyslmmalqlvwlis*ny*ikikisilfigsivfn*nvk
xikrec


Frame C:
---iildf*slilvmlwvildwsdpvvytivvmlsefvpdlkkipkfqdltskdalspet
iqg*yqfgfnhs*sfk*firgy*il*dvgkrfryrvskyr*stxkefxyhrtclglklhr
sydq**r*tl*eiksnrc*sfiih**wlcnwfglylkiirsk*rfrfsslvrsysiem*n
xsken

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHM627 (VHM627Q) /CSM/VH/VHM6-B/VHM627Q.Seq.d/                       1068   0.0
VHQ112 (VHQ112Q) /CSM/VH/VHQ1-A/VHQ112Q.Seq.d/                       1029   0.0
VHP871 (VHP871Q) /CSM/VH/VHP8-C/VHP871Q.Seq.d/                       1029   0.0
VHP507 (VHP507Q) /CSM/VH/VHP5-A/VHP507Q.Seq.d/                       1029   0.0
VHM639 (VHM639Q) /CSM/VH/VHM6-B/VHM639Q.Seq.d/                       1029   0.0
VHL410 (VHL410Q) /CSM/VH/VHL4-A/VHL410Q.Seq.d/                       1029   0.0
VHK575 (VHK575Q) /CSM/VH/VHK5-D/VHK575Q.Seq.d/                       1029   0.0
VHK538 (VHK538Q) /CSM/VH/VHK5-B/VHK538Q.Seq.d/                       1029   0.0
VHK464 (VHK464Q) /CSM/VH/VHK4-C/VHK464Q.Seq.d/                       1029   0.0
VHK424 (VHK424Q) /CSM/VH/VHK4-A/VHK424Q.Seq.d/                       1029   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BU746157|BU746157.1 CH3#002_C07T7 Canine heart normalized cDNA Library in pBluescriptCanis familiaris cDNA clone CH3#002_C07 5', mRNA sequence.    38   0.005  2
BY464171|BY464171.1 Mus musculus 15 days pregnant adult female amnion cDNA, RIKEN full-length enriched library, clone:K630150M24, 3' end partial sequence.    42   0.007  2
BX101185|BX101185.1 RZPD Homo sapiens cDNA clone IMAGp998E05583 = IMAGE:266092 5' EST.    40   0.016  2
AL627250|AL627250.8 Human DNA sequence from clone RP13-222H17 on chromosome X Contains a novel pseudogene.    40   0.020  6
BM802928|BM802928.1 AGENCOURT_6457255 NIH_MGC_88 Homo sapiens cDNA clone IMAGE:5560300 5', mRNA sequence.    40   0.024  2
BC040936|BC040936.1 Homo sapiens, clone IMAGE:5139212, mRNA, partial cds.    40   0.049  2
AX210657|AX210657.1 Sequence 299 from Patent WO0157058.            40   0.10   2
AB028956|AB028956.1 Homo sapiens mRNA for KIAA1033 protein, partial cds.    40   0.10   2
AL137753|AL137753.1 Homo sapiens mRNA; cDNA DKFZp434K1412 (from clone DKFZp434K1412).    40   0.11   2
AV854054|AV854054.1 Ciona intestinalis cDNA, clone:rciad15e04, 3' end, single read.    48   0.14   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54R74) RecName: Full=UPF0681 protein;                               241   2e-78
AB028956_1(AB028956|pid:none) Homo sapiens mRNA for KIAA1033 pro...   130   5e-42
BC143373_1(BC143373|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   130   5e-42
BC040936_1(BC040936|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...   130   5e-42
(Q3UMB9) RecName: Full=UPF0681 protein KIAA1033;                      126   6e-41
AK145000_1(AK145000|pid:none) Mus musculus mammary gland RCB-052...   126   6e-41
BC020314_1(BC020314|pid:none) Mus musculus RIKEN cDNA A230046K03...   126   6e-41
AK161247_1(AK161247|pid:none) Mus musculus adult male testis cDN...   126   6e-41
CP000600_221(CP000600|pid:none) Ostreococcus lucimarinus CCE9901...    98   2e-27
BC110850_1(BC110850|pid:none) Homo sapiens KIAA1033, mRNA (cDNA ...    81   3e-27

 psg: 0.67  gvh: 0.36  alm: 0.37  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VHM627 is cyt

>VHM627Z.Seq
NNNNNNNNNNTAGATCATTTTAGACTTTTAATCACTCATATTGGTAATGCTATGGGTTAT
ATTAGATTGGTCAGATCCGGTGGTTTACACTATTGTAGTAATGCTATCAGAATTCGTACC
AGACTTGAAAAAGATTCCAAAATTCCAAGATTTAACCTCAAAAGATGCCCTCTCACCTGA
AACAATTCAAGGGTAGTACCAATTTGGATTCAATCATTCATAATCTTTCAAATAATTTAT
CAGAGGGTACTGAATACTTTAAGATGTTGGTAAACGTTTTCGNTACAGAGTTTCGAAATA
TCGCTAATCAACATNTAAAGAATTTNTATATCATCGTACCTGCCTTGGCCTCAAATTACA
TCGATCATATGATCAATAGTAAAGATAAACTCTTTAAGAAATCAAAAGCAATCGGTGCTG
AAGCTTTATTATTCACTGATGATGGCTTTGCAATTGGTTTGGCTTATATCTTAAAATTAT
TAGATCAAAATAAAGATTTCGATTCTCTTCATTGGTTCGATCGTATTCAATTGAAATGTG
AAACNGATCAAAAGAGAATGT