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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHM522
Library VH
(Link to library)
Clone ID VHM522
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15767-1
Original site URL
Representative seq. ID VHM522P
(Link to Original site)
Representative DNA sequence 
>VHM522 (VHM522Q) /CSM/VH/VHM5-A/VHM522Q.Seq.d/
ACTGTTGGCCTACTGGNAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAA
GAATAAAAAAAATATATACAATAAAATAAAAAAAACAAAATGTTCATTCAAAGAACATTT
TTAAAAGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCA
AAAGATAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAAT
TTCCAACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATT
GATCAAGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAA
AAGGCAAGAATTGGTTGGCCAGATGGTTTTAAGTATTTTTTTGTAGATAACCAAGCTGGT
GATTCAAAATCTGCCAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAAT
TGGAATGGAGAAGCATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAAGGT
TTCAATGATGTAAGCTATCCAGTTCXXXXXXXXXXCCATCACCCACGTTCAAGTTACATG
TGGTGGTTGTGAAACCTGTTCATACGCTACCGGTAAATGTGAACCAGATAGTTCACTTTG
TAATGATAACAATATTTGTACCATTGATATTTGTGTCCATGAAGGTATTCTCGATGGTCT
ACCACAAGGTAATTGTTCAAATACTCCAGTTGATTGTGGTGCCAATGATGAAGATAAATG
TAAAACTTGGTCTTGTGATCCAACTAAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGA
AGATAAAGGTAAATGTTTAGTTGGTACTTGTCAACCATCCACTGGTCAATGTGAATACTC
TGATAGAGTTTGTGATAATGGTGGTGCTTTCTGTGTTATTGGCCAATGTGATCAAAGACT
TGGTTGTTTAGTTTTCGATAGAGTTTGTTCATCTGATAATAGTAGATGTGAAGAAGGTGT
TTGTGTAAATGGTACTGAATCTGAAGAAGGTCATTGTAAATCTGTTAAATATGACCCATT
ACCATTCAATTGTAATACTGGTGCTGTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGT
CGCTGGTGCTGTTGCTCTTGGTATTTTCCTTTATGGTGGTAAAAAAGGTTATGACTATTG
GAAAGACTCAAGAAATATTTCAATGGGCTCATCAAATAGTATCCATTGTATAAGAACAAC
AAACGGAAGAGGTGTCAACCCAATGAGACGATCCAGCGCNNATNAACAAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
tvgllxknnk*kirkskilke*kkyiq*NKKNKMFIQRTFLKVLTLLSIVTVLVHGQTQP
KDKITLKAVIYDQHKFYNPNFQPKNEGEFVLTKGIVKSDIDQEKRIPVLNSMDANDSINK
KARIGWPDGFKYFFVDNQAGDSKSAKSGKNLPIQRDIELNWNGEAYEYSNSNYFPINGQG
FNDVSYPV---

---ITHVQVTCGGCETCSYATGKCEPDSSLCNDNNICTIDICVHEGILDGLPQGNCSNTP
VDCGANDEDKCKTWSCDPTKGGCQSTPVVCEDKGKCLVGTCQPSTGQCEYSDRVCDNGGA
FCVIGQCDQRLGCLVFDRVCSSDNSRCEEGVCVNGTESEEGHCKSVKYDPLPFNCNTGAV
VSTAVIAGSTVAGAVALGIFLYGGKKGYDYWKDSRNISMGSSNSIHCIRTTNGRGVNPMR
RSSAXXQI
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxknnk*kirkskilke*kkyiq*NKKNKMFIQRTFLKVLTLLSIVTVLVHGQTQP
KDKITLKAVIYDQHKFYNPNFQPKNEGEFVLTKGIVKSDIDQEKRIPVLNSMDANDSINK
KARIGWPDGFKYFFVDNQAGDSKSAKSGKNLPIQRDIELNWNGEAYEYSNSNYFPINGQG
FNDVSYPV---

---psptfklhvvvvkpvhtlpvnvnqivhfvmitifvplifvsmkvfsmvyhkvivqil
qlivvpmmkinvklglviqlkvavnqpqsfvkikvnv*lvlvnhplvnvntliefvimvv
lsvllanvikdlvv*fsiefvhliivdvkkvfv*mvlnlkkvivnllnmthyhsivilvl
lfqlllllvllslvllllvfsfmvvkkvmtigktqeifqwahqivsiv*eqqteevstq*
ddpaxxnk

Frame B:
llaywxkttnkk*eslky*knkkniynkikktkcsfkehf*kfllf*v**qfwsmdklnq
kiklh*rp*fmintsfiiqisnqkmrenlf*lrelsnqilikkkefqf*ivwmpmil*ik
rqelvgqmvlsifl*itklviqnlpnlvkifqfkeiss*igmekhmniviqitsqltdkv
smm*aiqf---

---hhprssymwwl*nlfiryr*m*tr*ftl***qylyh*ylcp*rysrwsttr*lfkys
s*lwcq**r*m*nlvl*sn*rwlsinpsrl*r*r*mfswylstihwsm*il**sl**wwc
flcywpm*sktwlfsfr*slfi****m*rrclckwy*i*rrsl*ic*i*pitiql*ywcc
cfnccycwfycrwcccswyfplww*krl*llerlkkyfnglik*yplyknnkrkrcqpne
tiqrxxtn

Frame C:
cwptgkkqqiknkkv*nikrikkiytik*kkqnvhsknifkssysfeysnsfgpwtnstk
r*nyiegrnl*stqvl*skfptkk*gricfn*gncqiry*srkknssfk*ygcq*fyk*k
gknwlarwf*vffcr*psw*fkicqiw*kssnskryrvklewrsi*i**fkllpn*rtrf
q*cklss---

---ITHVQVTCGGCETCSYATGKCEPDSSLCNDNNICTIDICVHEGILDGLPQGNCSNTP
VDCGANDEDKCKTWSCDPTKGGCQSTPVVCEDKGKCLVGTCQPSTGQCEYSDRVCDNGGA
FCVIGQCDQRLGCLVFDRVCSSDNSRCEEGVCVNGTESEEGHCKSVKYDPLPFNCNTGAV
VSTAVIAGSTVAGAVALGIFLYGGKKGYDYWKDSRNISMGSSNSIHCIRTTNGRGVNPMR
RSSAXXQI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHM522 (VHM522Q) /CSM/VH/VHM5-A/VHM522Q.Seq.d/                       2284   0.0
VHA321 (VHA321Q) /CSM/VH/VHA3-A/VHA321Q.Seq.d/                       1390   0.0
VHQ393 (VHQ393Q) /CSM/VH/VHQ3-D/VHQ393Q.Seq.d/                       1388   0.0
VHA622 (VHA622Q) /CSM/VH/VHA6-A/VHA622Q.Seq.d/                       1388   0.0
VHN636 (VHN636Q) /CSM/VH/VHN6-B/VHN636Q.Seq.d/                       1386   0.0
VHP613 (VHP613Q) /CSM/VH/VHP6-A/VHP613Q.Seq.d/                       1384   0.0
VHH724 (VHH724Q) /CSM/VH/VHH7-A/VHH724Q.Seq.d/                       1380   0.0
VHI218 (VHI218Q) /CSM/VH/VHI2-A/VHI218Q.Seq.d/                       1366   0.0
VHC809 (VHC809Q) /CSM/VH/VHC8-A/VHC809Q.Seq.d/                       1366   0.0
AHL481 (AHL481Q) /CSM/AH/AHL4-D/AHL481Q.Seq.d/                       1362   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ444364) Dictyostelium discoideum cDNA clone:ddv56k05, 3' ...  1443   0.0    1
(BJ446801) Dictyostelium discoideum cDNA clone:ddv63j23, 3' ...  1320   0.0    2
(BJ446243) Dictyostelium discoideum cDNA clone:ddv62i04, 3' ...  1320   0.0    2
(BJ436757) Dictyostelium discoideum cDNA clone:ddv32k06, 3' ...  1320   0.0    2
(BJ436421) Dictyostelium discoideum cDNA clone:ddv31j05, 3' ...  1320   0.0    2
(BJ445062) Dictyostelium discoideum cDNA clone:ddv58g10, 3' ...  1312   0.0    3
(BJ441275) Dictyostelium discoideum cDNA clone:ddv46p05, 3' ...  1312   0.0    2
(BJ441540) Dictyostelium discoideum cDNA clone:ddv47c06, 3' ...  1306   0.0    2
(BJ437985) Dictyostelium discoideum cDNA clone:ddv36b04, 3' ...  1302   0.0    2
(BJ354479) Dictyostelium discoideum cDNA clone:dda53b22, 3' ...  1302   0.0    2
dna update 2008.12. 1
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54VS2) RecName: Full=Protein psiP; Flags: Precursor;                492   e-137
(Q94494) RecName: Full=Protein psiH; Flags: Precursor;  &U67940_...   249   2e-64
(Q86HT1) RecName: Full=Protein psiL; Flags: Precursor;  &AC11695...   243   1e-62
(Q54P69) RecName: Full=Protein psiM; Flags: Precursor;                204   6e-51
(Q557E9) RecName: Full=Protein psiG; Flags: Precursor;  &AC11568...   200   1e-49
(Q54G85) RecName: Full=Protein psiJ; Flags: Precursor;                140   1e-31
(Q54CH8) RecName: Full=Protein psiN; Flags: Precursor;                127   1e-27
S44208(S44208) extracellular matrix protein B - Dictyostelium mi...    87   2e-15
(P11976) RecName: Full=Prestalk protein; AltName: Full=Extracell...    86   4e-15
AC116984_103(AC116984|pid:none) Dictyostelium discoideum chromos...    84   1e-14
protein update 2009. 7.17
PSORT 

 psg: 0.84  gvh: 0.70  alm: 0.27  top: 0.30  tms: 0.13  mit: 0.33  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 28.0 %: mitochondrial
	 12.0 %: plasma membrane
	  8.0 %: nuclear
	  8.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VHM522 is end
5' end seq. ID VHM522F
5' end seq. 
>VHM522F.Seq
ACTGTTGGCCTACTGGNAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAA
GAATAAAAAAAATATATACAATAAAATAAAAAAAACAAAATGTTCATTCAAAGAACATTT
TTAAAAGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCA
AAAGATAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAAT
TTCCAACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATT
GATCAAGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAA
AAGGCAAGAATTGGTTGGCCAGATGGTTTTAAGTATTTTTTTGTAGATAACCAAGCTGGT
GATTCAAAATCTGCCAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAAT
TGGAATGGAGAAGCATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAAGGT
TTCAATGATGTAAGCTATCCAGTTCNNNNNNNNNN
Length of 5' end seq. 575
3' end seq. ID VHM522Z
3' end seq. 
>VHM522Z.Seq
NNNNNNNNNNCCATCACCCACGTTCAAGTTACATGTGGTGGTTGTGAAACCTGTTCATAC
GCTACCGGTAAATGTGAACCAGATAGTTCACTTTGTAATGATAACAATATTTGTACCATT
GATATTTGTGTCCATGAAGGTATTCTCGATGGTCTACCACAAGGTAATTGTTCAAATACT
CCAGTTGATTGTGGTGCCAATGATGAAGATAAATGTAAAACTTGGTCTTGTGATCCAACT
AAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGAAGATAAAGGTAAATGTTTAGTTGGT
ACTTGTCAACCATCCACTGGTCAATGTGAATACTCTGATAGAGTTTGTGATAATGGTGGT
GCTTTCTGTGTTATTGGCCAATGTGATCAAAGACTTGGTTGTTTAGTTTTCGATAGAGTT
TGTTCATCTGATAATAGTAGATGTGAAGAAGGTGTTTGTGTAAATGGTACTGAATCTGAA
GAAGGTCATTGTAAATCTGTTAAATATGACCCATTACCATTCAATTGTAATACTGGTGCT
GTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGTCGCTGGTGCTGTTGCTCTTGGTATT
TTCCTTTATGGTGGTAAAAAAGGTTATGACTATTGGAAAGACTCAAGAAATATTTCAATG
GGCTCATCAAATAGTATCCATTGTATAAGAACAACAAACGGAAGAGGTGTCAACCCAATG
AGACGATCCAGCGCNNATNAACAAATT
Length of 3' end seq. 747
Connected seq. ID VHM522P
Connected seq. 
>VHM522P.Seq
ACTGTTGGCCTACTGGNAAAAAACAACAAATAAAAAATAAGAAAGTCTAAAATATTAAAA
GAATAAAAAAAATATATACAATAAAATAAAAAAAACAAAATGTTCATTCAAAGAACATTT
TTAAAAGTTCTTACTCTTTTGAGTATAGTAACAGTTTTGGTCCATGGACAAACTCAACCA
AAAGATAAAATTACATTGAAGGCCGTAATTTATGATCAACACAAGTTTTATAATCCAAAT
TTCCAACCAAAAAATGAGGGAGAATTTGTTTTAACTAAGGGAATTGTCAAATCAGATATT
GATCAAGAAAAAAGAATTCCAGTTTTAAATAGTATGGATGCCAATGATTCTATAAATAAA
AAGGCAAGAATTGGTTGGCCAGATGGTTTTAAGTATTTTTTTGTAGATAACCAAGCTGGT
GATTCAAAATCTGCCAAATCTGGTAAAAATCTTCCAATTCAAAGAGATATCGAGTTAAAT
TGGAATGGAGAAGCATATGAATATAGTAATTCAAATTACTTCCCAATTAACGGACAAGGT
TTCAATGATGTAAGCTATCCAGTTC----------CCATCACCCACGTTCAAGTTACATG
TGGTGGTTGTGAAACCTGTTCATACGCTACCGGTAAATGTGAACCAGATAGTTCACTTTG
TAATGATAACAATATTTGTACCATTGATATTTGTGTCCATGAAGGTATTCTCGATGGTCT
ACCACAAGGTAATTGTTCAAATACTCCAGTTGATTGTGGTGCCAATGATGAAGATAAATG
TAAAACTTGGTCTTGTGATCCAACTAAAGGTGGCTGTCAATCAACCCCAGTCGTTTGTGA
AGATAAAGGTAAATGTTTAGTTGGTACTTGTCAACCATCCACTGGTCAATGTGAATACTC
TGATAGAGTTTGTGATAATGGTGGTGCTTTCTGTGTTATTGGCCAATGTGATCAAAGACT
TGGTTGTTTAGTTTTCGATAGAGTTTGTTCATCTGATAATAGTAGATGTGAAGAAGGTGT
TTGTGTAAATGGTACTGAATCTGAAGAAGGTCATTGTAAATCTGTTAAATATGACCCATT
ACCATTCAATTGTAATACTGGTGCTGTTGTTTCAACTGCTGTTATTGCTGGTTCTACTGT
CGCTGGTGCTGTTGCTCTTGGTATTTTCCTTTATGGTGGTAAAAAAGGTTATGACTATTG
GAAAGACTCAAGAAATATTTCAATGGGCTCATCAAATAGTATCCATTGTATAAGAACAAC
AAACGGAAGAGGTGTCAACCCAATGAGACGATCCAGCGCNNATNAACAAATT
Length of connected seq. 1302
Full length Seq ID -
Full length Seq. -
Length of full length seq. -