>AFO558 (AFO558Q) /CSM/AF/AFO5-C/AFO558Q.Seq.d/
XXXXXXXXXXGGGTGTAACATTTACACTACAATATGAAGATGTTAAAGGAATTTCAGAAG
CTTTGGATAAATTTGAAAAAGAAACCGGACCTTGATATACCAATTCATGTCGATGCAGCA
AGTGGTGGTTTCGTTGCACCCTTTCTTCAACCAGAAATTGTTTGGGATTTTAGACTACCA
CGTGTTAAATCAATTAATGGCTCTGGTCATAAATTTGGTCTATCTCCATTGGGAGTTGGC
TGGGTCGTATGGAGAGGTAAAAATGACATTCACAAGGATTTGGTATTCGATGTCAACTAT
CTTGGTGGCAATATGCCAACATTTTCATTGAATTTCTCACGTCCAGGTGGTCAAATAGTA
TGCCAGTACTATAACTTTTTGCGCCATGGTCGTAGTGGATATACCGCAGTTCATCAAGCT
TGTCTAGATCATGGTATTTTCATTTCCAAGCAAGTTAAAAAACACGGTATTTTTGATATT
GTTTATGATGGTACTGGTGCACTTCCAGGTGCATGTTGGAAATTGAAAAAAGATGCAAAA
GTAAAATTCAATCTCTTTGACCTTTCAGATAGAATGAGAAATAGAGGTTGGCAAATTGCA
TCCTATACTCTACCAAACTTACCAGATATTGTAGTTCAAAGAGTATTAATTAGACATGGT
TTCAGTCATTA

---v*HLHYNMKMLKEFQKLWINLKKKPDLDIPIHVDAASGGFVAPFLQPEIVWDFRLPR
VKSINGSGHKFGLSPLGVGWVVWRGKNDIHKDLVFDVNYLGGNMPTFSLNFSRPGGQIVC
QYYNFLRHGRSGYTAVHQACLDHGIFISKQVKKHGIFDIVYDGTGALPGACWKLKKDAKV
KFNLFDLSDRMRNRGWQIASYTLPNLPDIVVQRVLIRHGFSH

Frame A:
---gvtftlqyedvkgisealdkfeketgp*ytnscrcskwwfrctlsstrnclgf*ttt
c*in*wlws*iwsisigswlgrmer*k*hsqgfgircqlswwqyanifiefltsrwsnsm
pvl*lfapws*wiyrsssslsrswyfhfqas*ktryf*ycl*wywctsrcmleiekrcks
kiqsl*pfr*nek*rlancilystkltrycssksin*twfqsl


Frame B:
---v*HLHYNMKMLKEFQKLWINLKKKPDLDIPIHVDAASGGFVAPFLQPEIVWDFRLPR
VKSINGSGHKFGLSPLGVGWVVWRGKNDIHKDLVFDVNYLGGNMPTFSLNFSRPGGQIVC
QYYNFLRHGRSGYTAVHQACLDHGIFISKQVKKHGIFDIVYDGTGALPGACWKLKKDAKV
KFNLFDLSDRMRNRGWQIASYTLPNLPDIVVQRVLIRHGFSH


Frame C:
---cniytti*rc*rnfrsfg*i*krnrtliyqfmsmqqvvvslhpffnqklfgildyhv
lnqlmalvinlvylhwelagsygevkmtftriwysmstilvaicqhfh*ishvqvvk*ya
stitfcamvvvdipqfiklv*imvfsfpsklkntvflilfmmvlvhfqvhvgn*kkmqk*
nsisltfqie*eievgklhpilyqtyqil*fkey*ldmvsvi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO558 (AFO558Q) /CSM/AF/AFO5-C/AFO558Q.Seq.d/                       1310   0.0
CFI817 (CFI817Q) /CSM/CF/CFI8-A/CFI817Q.Seq.d/                       1294   0.0
SFG840 (SFG840Q) /CSM/SF/SFG8-B/SFG840Q.Seq.d/                       1291   0.0
SFD593 (SFD593Q) /CSM/SF/SFD5-D/SFD593Q.Seq.d/                       1291   0.0
SFD334 (SFD334Q) /CSM/SF/SFD3-B/SFD334Q.Seq.d/                       1291   0.0
CFF522 (CFF522Q) /CSM/CF/CFF5-A/CFF522Q.Seq.d/                       1291   0.0
SFF117 (SFF117Q) /CSM/SF/SFF1-A/SFF117Q.Seq.d/                       1279   0.0
SFE561 (SFE561Q) /CSM/SF/SFE5-C/SFE561Q.Seq.d/                       1277   0.0
SFC367 (SFC367Q) /CSM/SF/SFC3-C/SFC367Q.Seq.d/                       1275   0.0
SFG754 (SFG754Q) /CSM/SF/SFG7-C/SFG754Q.Seq.d/                       1271   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BG138757|BG138757.1 EST479199 wild tomato pollen Lycopersicon pennellii cDNA clone cLPP11A1 5' sequence, mRNA sequence.    66  1e-08  2
CD008053|CD008053.1 VVB082D05_335234 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB082D05 5, mRNA sequence.    64  2e-07  2
CD006712|CD006712.1 VVB066D06_332552 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB066D06 5, mRNA sequence.    64  2e-07  2
CD719214|CD719214.1 VVB158G03_410335 An expressed sequence tag database for abiotic stressed leaves of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVB158G03 5, mRNA sequence.    64  2e-07  2
CA813481|CA813481.1 CA48LN10IF-E8 Cabernet Sauvignon Leaf - CA48LN Vitis vinifera cDNA clone CA48LN10IF-E8 5', mRNA sequence.    64  3e-07  2
CB341828|CB341828.2 CA32EN0002_IIIbF_E01 Cabernet Sauvignon Leaf - CA32EN Vitis vinifera cDNA clone CA32EN0002_IIIbF_E01 5', mRNA sequence.    64  3e-07  2
AW622257|AW622257.1 EST313055 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX14P1 5', mRNA sequence.    66  6e-07  1
CB910900|CB910900.1 VVD157G07_379711 An expressed sequence tag database for abiotic stressed berries of Vitis vinifera var. Chardonnay Vitis vinifera cDNA clone VVD157G07 5, mRNA sequence.    64  2e-06  1
AF352732|AF352732.1 Nicotiana tabacum glutamate decarboxylase isozyme 3 mRNA, complete cds.    58  8e-06  2
AF353615|AF353615.1 Nicotiana tabacum glutamate decarboxylase isozyme 4 mRNA, complete cds.    60  8e-06  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FM178379_1197(FM178379|pid:none) Aliivibrio salmonicida LFI1238 ...   294   3e-84
CP000020_1061(CP000020|pid:none) Vibrio fischeri ES114 chromosom...   291   2e-83
CP000851_2941(CP000851|pid:none) Shewanella pealeana ATCC 700345...   286   6e-82
CR522870_385(CR522870|pid:none) Desulfotalea psychrophila LSv54 ...   278   2e-77
CP000789_2953(CP000789|pid:none) Vibrio harveyi ATCC BAA-1116 ch...   288   7e-77
CP001358_45(CP001358|pid:none) Desulfovibrio desulfuricans subsp...   288   1e-76
AM942759_1390(AM942759|pid:none) Proteus mirabilis strain HI4320...   269   1e-75
CP000439_1647(CP000439|pid:none) Francisella tularensis subsp. n...   267   8e-75
CP000608_56(CP000608|pid:none) Francisella tularensis subsp. tul...   264   7e-74
CP000937_903(CP000937|pid:none) Francisella philomiragia subsp. ...   258   2e-73

 psg: 0.72  gvh: 0.40  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.15  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal

>> prediction for AFO558 is nuc

>AFO558Z.Seq
----------GGGTGTAACATTTACACTACAATATGAAGATGTTAAAGGAATTTCAGAAG
CTTTGGATAAATTTGAAAAAGAAACCGGACCTTGATATACCAATTCATGTCGATGCAGCA
AGTGGTGGTTTCGTTGCACCCTTTCTTCAACCAGAAATTGTTTGGGATTTTAGACTACCA
CGTGTTAAATCAATTAATGGCTCTGGTCATAAATTTGGTCTATCTCCATTGGGAGTTGGC
TGGGTCGTATGGAGAGGTAAAAATGACATTCACAAGGATTTGGTATTCGATGTCAACTAT
CTTGGTGGCAATATGCCAACATTTTCATTGAATTTCTCACGTCCAGGTGGTCAAATAGTA
TGCCAGTACTATAACTTTTTGCGCCATGGTCGTAGTGGATATACCGCAGTTCATCAAGCT
TGTCTAGATCATGGTATTTTCATTTCCAAGCAAGTTAAAAAACACGGTATTTTTGATATT
GTTTATGATGGTACTGGTGCACTTCCAGGTGCATGTTGGAAATTGAAAAAAGATGCAAAA
GTAAAATTCAATCTCTTTGACCTTTCAGATAGAATGAGAAATAGAGGTTGGCAAATTGCA
TCCTATACTCTACCAAACTTACCAGATATTGTAGTTCAAAGAGTATTAATTAGACATGGT
TTCAGTCATTA