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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHM290
Library VH
(Link to library)
Clone ID VHM290
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHM290P
(Link to Original site)
Representative DNA sequence 
>VHM290 (VHM290Q) /CSM/VH/VHM2-D/VHM290Q.Seq.d/
ACTGTTGGCCTACTGGNAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTT
CACAATCGATCAAATTCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGT
TATTGCTCATGTCGATCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGG
TATTATTGCCGATAAAGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACA
AGAGCGTGGTATCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAACGCCAAAGGA
AGACAAATTACCAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCC
AGGTCACGTTGATTTCAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTT
AGTCGTTATCGATTGCGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGC
CGTCGCTGAACGTATCAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGA
ACTCCAATTAAACACTGAAGAAGCTTATCTCTCTTTCCGTANAGCTATTGAATCCGTCAA
TGTCATTGTTGGTAACACTGAXXXXXXXXXXTNACCAGTATTAGAATCCTTCGGTTTCAC
CGATGATTTACGTTCCCACAGTGCCGGTCAAGCTTTCCCACAATGTGTNTTNGATCATTG
GGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTNAGCCACCAG
AAAGAGAAAAGGTCTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTA
AACAATNTAAGTCGCACTTGAGTTTTCAAATATAGTA
sequence update 2002.10.25
Translated Amino Acid sequence 
LLAYWXLSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAG
IIADKVSGDMRYMSCRADEQERGITIKSSSVSLHFETPKEDKLPAGCTSHEFLINLIDSP
GHVDFSSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLE
LQLNTEEAYLSFRXAIESVNVIVGNT---

---XPVLESFGFTDDLRSHSAGQAFPQCVXDHWASIGVVNKDKKATEVAXATRKRKGLAP
EIPDLDKFHEKL*tx*valefsniv
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxtleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpksw
yycr*sfw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfp
rsr*fqfrsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflir
tpikh*rslslfpxsy*irqchcw*h*---

---XPVLESFGFTDDLRSHSAGQAFPQCVXDHWASIGVVNKDKKATEVAXATRKRKGLAP
EIPDLDKFHEKL*tx*valefsniv

Frame B:
LLAYWXLSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAG
IIADKVSGDMRYMSCRADEQERGITIKSSSVSLHFETPKEDKLPAGCTSHEFLINLIDSP
GHVDFSSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLE
LQLNTEEAYLSFRXAIESVNVIVGNT---

---xqy*npsvspmiyvptvpvklshnvxxiiglqlvlstkirkplkslxpperekv*pq
ksqiltnstknskqxkshlsfqi*

Frame C:
cwptgnsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelv
llpikflvi*dichvvlmnksvvsplnlhlyhftskrqrktnyqlvvlhtnf*silsipq
vtlisvqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*n
sn*tlkklislsvxllnpsmsllvtl---

---tsirilrfhr*ftfpqcrssfptmcxxslgfnwccqqr*esh*srxshqkekrsspr
nprs*qiprktlnnxsrt*vfkys
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHM290 (VHM290Q) /CSM/VH/VHM2-D/VHM290Q.Seq.d/                       1596   0.0
VHO558 (VHO558Q) /CSM/VH/VHO5-C/VHO558Q.Seq.d/                       1170   0.0
VHH458 (VHH458Q) /CSM/VH/VHH4-C/VHH458Q.Seq.d/                       1170   0.0
VHH143 (VHH143Q) /CSM/VH/VHH1-B/VHH143Q.Seq.d/                       1170   0.0
VHI860 (VHI860Q) /CSM/VH/VHI8-C/VHI860Q.Seq.d/                       1160   0.0
VHB380 (VHB380Q) /CSM/VH/VHB3-D/VHB380Q.Seq.d/                       1160   0.0
CHP421 (CHP421Q) /CSM/CH/CHP4-A/CHP421Q.Seq.d/                       1160   0.0
CHP293 (CHP293Q) /CSM/CH/CHP2-D/CHP293Q.Seq.d/                       1160   0.0
VHH565 (VHH565Q) /CSM/VH/VHH5-C/VHH565Q.Seq.d/                       1152   0.0
VHG544 (VHG544Q) /CSM/VH/VHG5-B/VHG544Q.Seq.d/                       1152   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ425454) Dictyostelium discoideum cDNA clone:ddv55c24, 5' ...  1152   0.0    2
(BJ422605) Dictyostelium discoideum cDNA clone:ddv46a17, 5' ...  1152   0.0    1
(BJ421942) Dictyostelium discoideum cDNA clone:ddv44g11, 5' ...  1152   0.0    1
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1144   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1144   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1144   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1144   0.0    1
(BJ427875) Dictyostelium discoideum cDNA clone:ddv63k24, 5' ...  1144   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1144   0.0    1
(BJ427873) Dictyostelium discoideum cDNA clone:ddv63k22, 5' ...  1144   0.0    1
dna update 2008.11.30
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   260   4e-68
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   255   2e-66
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   254   2e-66
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   253   6e-66
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   253   6e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   252   1e-65
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   251   2e-65
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   251   2e-65
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   251   2e-65
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   251   2e-65
protein update 2009. 7.16
PSORT 

 psg: 0.51  gvh: 0.35  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHM290 is cyt
5' end seq. ID VHM290F
5' end seq. 
>VHM290F.Seq
ACTGTTGGCCTACTGGNAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTT
CACAATCGATCAAATTCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGT
TATTGCTCATGTCGATCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGG
TATTATTGCCGATAAAGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACA
AGAGCGTGGTATCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAACGCCAAAGGA
AGACAAATTACCAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCC
AGGTCACGTTGATTTCAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTT
AGTCGTTATCGATTGCGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGC
CGTCGCTGAACGTATCAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGA
ACTCCAATTAAACACTGAAGAAGCTTATCTCTCTTTCCGTANAGCTATTGAATCCGTCAA
TGTCATTGTTGGTAACACTGANNNNNNNNNN
Length of 5' end seq. 631
3' end seq. ID VHM290Z
3' end seq. 
>VHM290Z.Seq
NNNNNNNNNNTNACCAGTATTAGAATCCTTCGGTTTCACCGATGATTTACGTTCCCACAG
TGCCGGTCAAGCTTTCCCACAATGTGTNTTNGATCATTGGGCTTCAATTGGTGTTGTCAA
CAAAGATAAGAAAGCCACTGAAGTCGCTTNAGCCACCAGAAAGAGAAAAGGTCTAGCCCC
AGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATNTAAGTCGCACTTGA
GTTTTCAAATATAGTA
Length of 3' end seq. 256
Connected seq. ID VHM290P
Connected seq. 
>VHM290P.Seq
ACTGTTGGCCTACTGGNAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTT
CACAATCGATCAAATTCGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGT
TATTGCTCATGTCGATCACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGG
TATTATTGCCGATAAAGTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACA
AGAGCGTGGTATCACCATTAAATCTTCATCTGTATCACTTCACTTCGAAACGCCAAAGGA
AGACAAATTACCAGCTGGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCC
AGGTCACGTTGATTTCAGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTT
AGTCGTTATCGATTGCGTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGC
CGTCGCTGAACGTATCAAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGA
ACTCCAATTAAACACTGAAGAAGCTTATCTCTCTTTCCGTANAGCTATTGAATCCGTCAA
TGTCATTGTTGGTAACACTGA----------TNACCAGTATTAGAATCCTTCGGTTTCAC
CGATGATTTACGTTCCCACAGTGCCGGTCAAGCTTTCCCACAATGTGTNTTNGATCATTG
GGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTNAGCCACCAG
AAAGAGAAAAGGTCTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTA
AACAATNTAAGTCGCACTTGAGTTTTCAAATATAGTA
Length of connected seq. 867
Full length Seq ID -
Full length Seq. -
Length of full length seq. -