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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHM195
Library VH
(Link to library)
Clone ID VHM195
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12060-1|Contig-U12475-1
Original site URL
Representative seq. ID VHM195P
(Link to Original site)
Representative DNA sequence 
>VHM195 (VHM195Q) /CSM/VH/VHM1-D/VHM195Q.Seq.d/
CAGATACATTGATACCAGTTGCAAAGAAAATTTGGACCCAATATGATAGACCTGAATACT
ATGGTAAACTCAAATTACAACCAATCATAACCTGGGTATTCGATGGTGCCGAAAGTTGCT
TATTCAAGAGTAAATTCAGTAAATGGGTAGAGAAAGCACAACCATTACAAACCTCCTACT
TGTCATTATCCTCAAAGAAAAAGGAGGCTTTAAATTTCGATGTATCCTCAATGCATCAAG
ATAAAGAAGTACCAGTGATTAACATTGGCGCTGCATCAGATTATAGTAATGGTAAACTAT
TGGTATGGTGTTCTGGTGGTGGCAGTGGTAATAAATGGAATAAAGTGGAGGAAGACGATT
TTGGTATTTTCTATTCCAACAAATCATACGTTTGCCATTTCATTTATAAACCAGAGAATA
AGAATTCAATTCGTTCCGTGATCTTCTTTTGGGAGGGTCTATACTCTAACCAACGTAATT
ACATTGGCTACAAGTTTGGTCTCTACAAGGAGATTCAAAAGAAGATGGAAGGTTTGAAAT
CAGATGATCCCGTCGAATATAGAATCTCTCAAAATAAAGAACCACAAGAATTTATCAATC
TCTTTGGTACAXXXXXXXXXXAAGAAGAGGTTAAGNAGGAGGTTAAAGAAGTCAAAGAGG
AAGTCAAAGAAGAAGTTAAACAAGATAAAGAAGAGGAGGTTAACGAAGAAATCAAAGAAG
AAACTAAGGAAGAAGAAACTAAAGAAGATGATAATAAAGAGGATGAAAAAGTAAATGAAG
AAAATGAAACAGTAAATGAAGAAAATGAAGTTGGTATCATCGTATCACCACCATCAGAAA
AAGTTGATGAAGCCAATAGTTCTTCAACAATATCTTCACCAGAGAATGAAGGATCAGTAT
CGGTTAAAGACAAGAGAAAATCAAATGAACCTATAACTCCAAGTGTAGTCTCATCTAGTG
GTGACTTACTCTTTGCCTATGAACCATATCATGGCCCTCCAATTCATTCATCAAATAGTC
CCAAACAAGGTAGAAAAGGTGGAAGGAAATCACATGGTAAAAATCAACCACAACATAAAA
AAAACCATAGCGTTGATCAATCTCCATTATCTTCTCCAATCATTCCAAACAAATCTTTAA
ATTTAGATATCGATAACCAATCATTTGATTTAAATAGCATCAATAACAATAACTCTGTCG
AGGTTTTAGATGGCGCTTCATCACCACTTTCTTTCTCTTCCTCTTCAATNATTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
DTLIPVAKKIWTQYDRPEYYGKLKLQPIITWVFDGAESCLFKSKFSKWVEKAQPLQTSYL
SLSSKKKEALNFDVSSMHQDKEVPVINIGAASDYSNGKLLVWCSGGGSGNKWNKVEEDDF
GIFYSNKSYVCHFIYKPENKNSIRSVIFFWEGLYSNQRNYIGYKFGLYKEIQKKMEGLKS
DDPVEYRISQNKEPQEFINLFGT---

---EEVKXEVKEVKEEVKEEVKQDKEEEVNEEIKEETKEEETKEDDNKEDEKVNEENETV
NEENEVGIIVSPPSEKVDEANSSSTISSPENEGSVSVKDKRKSNEPITPSVVSSSGDLLF
AYEPYHGPPIHSSNSPKQGRKGGRKSHGKNQPQHKKNHSVDQSPLSSPIIPNKSLNLDID
NQSFDLNSINNNNSVEVLDGASSPLSFSSSSXIQ
Translated Amino Acid sequence (All Frames) 
Frame A:
qih*yqlqrkfgpnmidlntmvnsnynqs*pgysmvpkvaysrvnsvng*rkhnhykppt
chypqrkrrl*ismypqcikikkyq*ltlalhqiivmvnywygvlvvavvingikwrkti
lvfsiptnhtfaisfinqririqfvp*ssfgrvytltnvitlatslvstrrfkrrwkv*n
qmipsnieslkiknhknlsislv---

---kkrlxrrlkkskrkskkklnkikkrrltkkskkklrkkklkkmiikrmkk*mkkmkq
*mkkmklvssyhhhqkklmkpivlqqylhqrmkdqyrlktrenqmnl*lqv*shlvvtys
lpmnhimalqfihqivpnkvekvegnhmvkinhnikktialinlhyllqsfqtnl*i*is
itnhli*iasititlsrf*malhhhflslplqxfk

Frame B:
ryidtsckenldpi**t*ilw*tqittnhnlgirwcrklliqe*iq*mgresttitnlll
viilkekggfkfrcilnasr*rstsd*hwrcirl**w*tigmvfwwwqw**me*sggrrf
wyflfqqiirlpfhl*tre*efnsfrdlllggsil*pt*lhwlqvwslqgdskedgrfei
r*srri*nlsk*rttriyqslwy---

---rrg*xgg*rsqrgsqrrs*tr*rrgg*rrnqrrn*grrn*rr***rg*ksk*rk*ns
k*rk*swyhrittirks**sq*ffnniftre*risig*rqekik*tynskcsli*w*ltl
cl*tiswpsnsfik*sqtr*krwkeitw*ksttt*kkp*r*sisiifsnhskqifkfryr
*pii*fk*hq*q*lcrgfrwrfittfflflfnxs

Frame C:
DTLIPVAKKIWTQYDRPEYYGKLKLQPIITWVFDGAESCLFKSKFSKWVEKAQPLQTSYL
SLSSKKKEALNFDVSSMHQDKEVPVINIGAASDYSNGKLLVWCSGGGSGNKWNKVEEDDF
GIFYSNKSYVCHFIYKPENKNSIRSVIFFWEGLYSNQRNYIGYKFGLYKEIQKKMEGLKS
DDPVEYRISQNKEPQEFINLFGT---

---EEVKXEVKEVKEEVKEEVKQDKEEEVNEEIKEETKEEETKEDDNKEDEKVNEENETV
NEENEVGIIVSPPSEKVDEANSSSTISSPENEGSVSVKDKRKSNEPITPSVVSSSGDLLF
AYEPYHGPPIHSSNSPKQGRKGGRKSHGKNQPQHKKNHSVDQSPLSSPIIPNKSLNLDID
NQSFDLNSINNNNSVEVLDGASSPLSFSSSSXIQ
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHM195 (VHM195Q) /CSM/VH/VHM1-D/VHM195Q.Seq.d/                       1330   0.0
VFM161 (VFM161Q) /CSM/VF/VFM1-C/VFM161Q.Seq.d/                       1142   0.0
VHG175 (VHG175Q) /CSM/VH/VHG1-D/VHG175Q.Seq.d/                        946   0.0
CHG579 (CHG579Q) /CSM/CH/CHG5-D/CHG579Q.Seq.d/                        801   0.0
VHE582 (VHE582Q) /CSM/VH/VHE5-D/VHE582Q.Seq.d/                        743   0.0
VHG275 (VHG275Q) /CSM/VH/VHG2-D/VHG275Q.Seq.d/                        737   0.0
VHG783 (VHG783Q) /CSM/VH/VHG7-D/VHG783Q.Seq.d/                        446   e-124
VSA282 (VSA282Q) /CSM/VS/VSA2-D/VSA282Q.Seq.d/                        321   1e-86
VSJ652 (VSJ652Q) /CSM/VS/VSJ6-C/VSJ652Q.Seq.d/                         42   0.020
AFK221 (AFK221Q) /CSM/AF/AFK2-A/AFK221Q.Seq.d/                         42   0.020
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ425501) Dictyostelium discoideum cDNA clone:ddv55m23, 5' ...  1211   0.0    1
(U95159) Dictyostelium discoideum gelsolin-related protein G...  1209   0.0    1
(BJ416275) Dictyostelium discoideum cDNA clone:ddv25i15, 5' ...  1142   0.0    1
(BJ444301) Dictyostelium discoideum cDNA clone:ddv55m23, 3' ...   527   0.0    2
(BJ440470) Dictyostelium discoideum cDNA clone:ddv43e19, 3' ...   527   0.0    2
(BJ421772) Dictyostelium discoideum cDNA clone:ddv43e19, 5' ...   821   0.0    1
(BJ420889) Dictyostelium discoideum cDNA clone:ddv40c21, 5' ...   743   0.0    1
(BJ379764) Dictyostelium discoideum cDNA clone:ddc35m19, 3' ...   527   0.0    2
(BJ440471) Dictyostelium discoideum cDNA clone:ddv43e20, 3' ...   527   e-174  2
(BJ440812) Dictyostelium discoideum cDNA clone:ddv44f21, 3' ...   446   e-121  1
dna update 2008.11.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

T30330(T30330) gelsolin-related protein GRP125 - slime mold (Dic...   426   e-117
AC114263_37(AC114263|pid:none) Dictyostelium discoideum chromoso...    84   1e-14
BX927121_2(BX927121|pid:none) Zebrafish DNA sequence from clone ...    63   2e-08
BC066531_1(BC066531|pid:none) Danio rerio scinderin like b, mRNA...    63   2e-08
BC155581_1(BC155581|pid:none) Danio rerio scinderin like a, mRNA...    62   4e-08
BC125898_1(BC125898|pid:none) Danio rerio scinderin like a, mRNA...    62   4e-08
AF175294_1(AF175294|pid:none) Danio rerio gelsolin mRNA, complet...    62   4e-08
AL513122_7(AL513122|pid:none) Human DNA sequence from clone RP11...    60   2e-07
(P06396) RecName: Full=Gelsolin; AltName: Full=Actin-depolymeriz...    60   2e-07
CR859411_1(CR859411|pid:none) Pongo abelii mRNA; cDNA DKFZp469O1...    60   2e-07
protein update 2009. 7.16
PSORT 

 psg: 0.54  gvh: 0.36  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHM195 is nuc
5' end seq. ID VHM195F
5' end seq. 
>VHM195F.Seq
CAGATACATTGATACCAGTTGCAAAGAAAATTTGGACCCAATATGATAGACCTGAATACT
ATGGTAAACTCAAATTACAACCAATCATAACCTGGGTATTCGATGGTGCCGAAAGTTGCT
TATTCAAGAGTAAATTCAGTAAATGGGTAGAGAAAGCACAACCATTACAAACCTCCTACT
TGTCATTATCCTCAAAGAAAAAGGAGGCTTTAAATTTCGATGTATCCTCAATGCATCAAG
ATAAAGAAGTACCAGTGATTAACATTGGCGCTGCATCAGATTATAGTAATGGTAAACTAT
TGGTATGGTGTTCTGGTGGTGGCAGTGGTAATAAATGGAATAAAGTGGAGGAAGACGATT
TTGGTATTTTCTATTCCAACAAATCATACGTTTGCCATTTCATTTATAAACCAGAGAATA
AGAATTCAATTCGTTCCGTGATCTTCTTTTGGGAGGGTCTATACTCTAACCAACGTAATT
ACATTGGCTACAAGTTTGGTCTCTACAAGGAGATTCAAAAGAAGATGGAAGGTTTGAAAT
CAGATGATCCCGTCGAATATAGAATCTCTCAAAATAAAGAACCACAAGAATTTATCAATC
TCTTTGGTACANNNNNNNNNN
Length of 5' end seq. 621
3' end seq. ID VHM195Z
3' end seq. 
>VHM195Z.Seq
NNNNNNNNNNAAGAAGAGGTTAAGNAGGAGGTTAAAGAAGTCAAAGAGGAAGTCAAAGAA
GAAGTTAAACAAGATAAAGAAGAGGAGGTTAACGAAGAAATCAAAGAAGAAACTAAGGAA
GAAGAAACTAAAGAAGATGATAATAAAGAGGATGAAAAAGTAAATGAAGAAAATGAAACA
GTAAATGAAGAAAATGAAGTTGGTATCATCGTATCACCACCATCAGAAAAAGTTGATGAA
GCCAATAGTTCTTCAACAATATCTTCACCAGAGAATGAAGGATCAGTATCGGTTAAAGAC
AAGAGAAAATCAAATGAACCTATAACTCCAAGTGTAGTCTCATCTAGTGGTGACTTACTC
TTTGCCTATGAACCATATCATGGCCCTCCAATTCATTCATCAAATAGTCCCAAACAAGGT
AGAAAAGGTGGAAGGAAATCACATGGTAAAAATCAACCACAACATAAAAAAAACCATAGC
GTTGATCAATCTCCATTATCTTCTCCAATCATTCCAAACAAATCTTTAAATTTAGATATC
GATAACCAATCATTTGATTTAAATAGCATCAATAACAATAACTCTGTCGAGGTTTTAGAT
GGCGCTTCATCACCACTTTCTTTCTCTTCCTCTTCAATNATTCAAA
Length of 3' end seq. 646
Connected seq. ID VHM195P
Connected seq. 
>VHM195P.Seq
CAGATACATTGATACCAGTTGCAAAGAAAATTTGGACCCAATATGATAGACCTGAATACT
ATGGTAAACTCAAATTACAACCAATCATAACCTGGGTATTCGATGGTGCCGAAAGTTGCT
TATTCAAGAGTAAATTCAGTAAATGGGTAGAGAAAGCACAACCATTACAAACCTCCTACT
TGTCATTATCCTCAAAGAAAAAGGAGGCTTTAAATTTCGATGTATCCTCAATGCATCAAG
ATAAAGAAGTACCAGTGATTAACATTGGCGCTGCATCAGATTATAGTAATGGTAAACTAT
TGGTATGGTGTTCTGGTGGTGGCAGTGGTAATAAATGGAATAAAGTGGAGGAAGACGATT
TTGGTATTTTCTATTCCAACAAATCATACGTTTGCCATTTCATTTATAAACCAGAGAATA
AGAATTCAATTCGTTCCGTGATCTTCTTTTGGGAGGGTCTATACTCTAACCAACGTAATT
ACATTGGCTACAAGTTTGGTCTCTACAAGGAGATTCAAAAGAAGATGGAAGGTTTGAAAT
CAGATGATCCCGTCGAATATAGAATCTCTCAAAATAAAGAACCACAAGAATTTATCAATC
TCTTTGGTACA----------AAGAAGAGGTTAAGNAGGAGGTTAAAGAAGTCAAAGAGG
AAGTCAAAGAAGAAGTTAAACAAGATAAAGAAGAGGAGGTTAACGAAGAAATCAAAGAAG
AAACTAAGGAAGAAGAAACTAAAGAAGATGATAATAAAGAGGATGAAAAAGTAAATGAAG
AAAATGAAACAGTAAATGAAGAAAATGAAGTTGGTATCATCGTATCACCACCATCAGAAA
AAGTTGATGAAGCCAATAGTTCTTCAACAATATCTTCACCAGAGAATGAAGGATCAGTAT
CGGTTAAAGACAAGAGAAAATCAAATGAACCTATAACTCCAAGTGTAGTCTCATCTAGTG
GTGACTTACTCTTTGCCTATGAACCATATCATGGCCCTCCAATTCATTCATCAAATAGTC
CCAAACAAGGTAGAAAAGGTGGAAGGAAATCACATGGTAAAAATCAACCACAACATAAAA
AAAACCATAGCGTTGATCAATCTCCATTATCTTCTCCAATCATTCCAAACAAATCTTTAA
ATTTAGATATCGATAACCAATCATTTGATTTAAATAGCATCAATAACAATAACTCTGTCG
AGGTTTTAGATGGCGCTTCATCACCACTTTCTTTCTCTTCCTCTTCAATNATTCAAA
Length of connected seq. 1247
Full length Seq ID -
Full length Seq. -
Length of full length seq. -