>AFO547 (AFO547Q) /CSM/AF/AFO5-B/AFO547Q.Seq.d/
ACTCATCTTTTAAAGGGTAAATTGATAATGATCAATTTCTTTGTACAATAGAATATAAAA
TTTAATATATATATGAGACTAGTATTTCAATAAATCATCTAAAAAAAATAAATTTTTAAA
GATAGTTGAAATTAAAAAAAAAAXXXXXXXXXXAANGTGTTGANTGGGCATTGGCNCTTC
ATTCATTACCAAANAAAACCNTTGGCAAAAANANGGGNATTTGGCANATNTNTTTTATCG
CNCCNACTGNAGNTGNNCCANCTANCNCTGGNAGCATNTTACAAGCTACNCAAATTACCC
NTGAAAANGGGACTAAAANTTNTTCC

lif*rvn***sislynri*nliyi*d*YFNKSSKKNKFLKIVEIKKK---

---XVLXGHWXFIHYQXKPLAKXXXFGXXXLSXXLXXXXLXLXAXYKLXKLPXKXGLKXX

Frame A:
thllkgkliminffvq*nikfniymrlvfq*ii*kk*ifkds*n*kk---

---XVLXGHWXFIHYQXKPLAKXXXFGXXXLSXXLXXXXLXLXAXYKLXKLPXKXGLKXX

Frame B:
lif*rvn***sislynri*nliyi*d*YFNKSSKKNKFLKIVEIKKK---

---xc*xgigxsfitkxnxwqkxgxlaxxfyrxxxxxxxxxwxhxtsyxnyp*kxd*xxf

Frame C:
ssfkg*idndqflctieyki*yiyetsisinhlkkinf*r*lklkkk---

---xvxwalalhslpxktxgkxxgiwxxxfiaptxxxpxxxgsxlqatqitxexgtkxxs

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO547 (AFO547Q) /CSM/AF/AFO5-B/AFO547Q.Seq.d/                        216   2e-55
AFO283 (AFO283Q) /CSM/AF/AFO2-D/AFO283Q.Seq.d/                        216   2e-55
AFM487 (AFM487Q) /CSM/AF/AFM4-D/AFM487Q.Seq.d/                        216   2e-55
AFH507 (AFH507Q) /CSM/AF/AFH5-A/AFH507Q.Seq.d/                        216   2e-55
SSM658 (SSM658Q) /CSM/SS/SSM6-C/SSM658Q.Seq.d/                        198   4e-50
SSM685 (SSM685Q) /CSM/SS/SSM6-D/SSM685Q.Seq.d/                        165   5e-40
AFJ322 (AFJ322Q) /CSM/AF/AFJ3-A/AFJ322Q.Seq.d/                        165   5e-40
SLH171 (SLH171Q) /CSM/SL/SLH1-C/SLH171Q.Seq.d/                        157   1e-37
AFN759 (AFN759Q) /CSM/AF/AFN7-C/AFN759Q.Seq.d/                        155   5e-37
AFI202 (AFI202Q) /CSM/AF/AFI2-A/AFI202Q.Seq.d/                        103   2e-21

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BH526395|BH526395.1 BOHJW56TF BOHJ Brassica oleracea genomic clone BOHJW56, DNA sequence.    46  0.26  1
BZ078426|BZ078426.1 llf25g09.b1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    46  0.26  1
BX557543|BX557543.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse33b05_p1c.    46  0.26  1
AC034163|AC034163.4 Homo sapiens chromosome 5 clone RP11-414I19, WORKING DRAFT SEQUENCE, 43 unordered pieces.    44  1.0  1
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.           44  1.0  1
AC016910|AC016910.6 Homo sapiens BAC clone RP11-460B1 from 2, complete sequence.    44  1.0  1
AC007935|AC007935.3 Homo sapiens clone RP11-49A1, WORKING DRAFT SEQUENCE, 25 unordered pieces.    44  1.0  1
AC006281|AC006281.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    42  1.5  2
AE014845|AE014845.1 Plasmodium falciparum 3D7 chromosome 12, section 2 of 9 of the complete sequence.    42  1.8  2
AC109474|AC109474.2 Homo sapiens chromosome 5 clone RP11-414I19, complete sequence.    44  3.9  4

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 261,452,514

 psg: 0.65  gvh: 0.58  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for AFO547 is nuc

>AFO547F.Seq
ACTCATCTTTTAAAGGGTAAATTGATAATGATCAATTTCTTTGTACAATAGAATATAAAA
TTTAATATATATATGAGACTAGTATTTCAATAAATCATCTAAAAAAAATAAATTTTTAAA
GATAGTTGAAATTAAAAAAAAAA----------

>AFO547Z.Seq
----------AANGTGTTGANTGGGCATTGGCNCTTCATTCATTACCAAANAAAACCNTT
GGCAAAAANANGGGNATTTGGCANATNTNTTTTATCGCNCCNACTGNAGNTGNNCCANCT
ANCNCTGGNAGCATNTTACAAGCTACNCAAATTACCCNTGAAAANGGGACTAAAANTTNT
TCC

>AFO547P.Seq
ACTCATCTTTTAAAGGGTAAATTGATAATGATCAATTTCTTTGTACAATAGAATATAAAA
TTTAATATATATATGAGACTAGTATTTCAATAAATCATCTAAAAAAAATAAATTTTTAAA
GATAGTTGAAATTAAAAAAAAAA----------AANGTGTTGANTGGGCATTGGCNCTTC
ATTCATTACCAAANAAAACCNTTGGCAAAAANANGGGNATTTGGCANATNTNTTTTATCG
CNCCNACTGNAGNTGNNCCANCTANCNCTGGNAGCATNTTACAAGCTACNCAAATTACCC
NTGAAAANGGGACTAAAANTTNTTCC