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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHM121
Library VH
(Link to library)
Clone ID VHM121
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16496-1
Original site URL
Representative seq. ID VHM121P
(Link to Original site)
Representative DNA sequence 
>VHM121 (VHM121Q) /CSM/VH/VHM1-A/VHM121Q.Seq.d/
ATCAACACCAATACAGTAAATTTAAATAAAGAAATAGATTAAAGTTTTCCATCTATTTAT
TTATTTTCCTCAATTTACAATTTTTCAAATTAATTAATACAATATTAAAGTTAAAAGAAA
AAAAAAAXXXXXXXXXXACAACCGCCACAGAAATNACAAGCNGCAGTATCTCAAGTAGCA
TTAGTAGTACCTNTGGTTTGAATGAGGATGAAATTGACGAGTCTGCAATNGAAGCCATCG
CCGCCGGTATCTCTCATTTACCAACCTACTCACCAACTAGTTTCTTTGATAGCATTTCAT
GTGAAGCTTTAGATAAGGCAAACGAAGGTCAAGGTAAATCAAAATCACTNTATGAACAAA
GAAAAATCGATCAAGAAACCTTTGGTGTTTTATCTGTTAGATATGGTAGAGGTGGTAGAG
GTGGAAGAGGTGGTAGANATAATAATAGTCACAGAC
sequence update 2002.10.25
Translated Amino Acid sequence 
qhqyskfk*RNRLKFSIYLFIFLNLQFFKLINTILKLKEKK---

---TTATEXTSXSISSSISSTXGLNEDEIDESAXEAIAAGISHLPTYSPTSFFDSISCEA
LDKANEGQGKSKSLYEQRKIDQETFGVLSVRYGRGGRGGRGGRXNNSHR
Translated Amino Acid sequence (All Frames) 
Frame A:
intntvnlnkeid*sfpsiylfssiynfsn*liqy*s*kkkk---

---TTATEXTSXSISSSISSTXGLNEDEIDESAXEAIAAGISHLPTYSPTSFFDSISCEA
LDKANEGQGKSKSLYEQRKIDQETFGVLSVRYGRGGRGGRGGRXNNSHR

Frame B:
stpiq*i*ikk*ikvfhlfiyfpqftifqin*ynikvkrkkk---

---qppqkxqaavsqvalvvpxv*mrmkltslqxkpsppvsliyqpthqlvsliafhvkl
*irqtkvkvnqnhxmnkeksikkplvfylldmvevveveevvxiiivtd

Frame C:
qhqyskfk*RNRLKFSIYLFIFLNLQFFKLINTILKLKEKK---

---nrhrnxkxqylk*h**ylwfe*g*n*rvcnxshrrrylsftnlltn*fl**hfm*sf
r*gkrrsr*ikitx*tkknrsrnlwcfic*iw*rw*rwkrw*x***sq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHM121 (VHM121Q) /CSM/VH/VHM1-A/VHM121Q.Seq.d/                        597   e-170
SLH711 (SLH711Q) /CSM/SL/SLH7-A/SLH711Q.Seq.d/                        571   e-162
SLG614 (SLG614Q) /CSM/SL/SLG6-A/SLG614Q.Seq.d/                        571   e-162
SHG474 (SHG474Q) /CSM/SH/SHG4-D/SHG474Q.Seq.d/                        571   e-162
VFC401 (VFC401Q) /CSM/VF/VFC4-A/VFC401Q.Seq.d/                        537   e-152
CHL567 (CHL567Q) /CSM/CH/CHL5-C/CHL567Q.Seq.d/                        494   e-139
VSJ357 (VSJ357Q) /CSM/VS/VSJ3-C/VSJ357Q.Seq.d/                        470   e-132
VHK710 (VHK710Q) /CSM/VH/VHK7-A/VHK710Q.Seq.d/                        232   4e-60
VFE829 (VFE829Q) /CSM/VF/VFE8-B/VFE829Q.Seq.d/                        224   9e-58
SHI327 (SHI327Q) /CSM/SH/SHI3-B/SHI327Q.Seq.d/                        214   9e-55
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ444066) Dictyostelium discoideum cDNA clone:ddv55i05, 3' ...   597   e-166  1
(AU039863) Dictyostelium discoideum slug cDNA, clone SLG614.      571   e-159  1
(AU039398) Dictyostelium discoideum slug cDNA, clone SLH711.      571   e-159  1
(BJ406888) Dictyostelium discoideum cDNA clone:dds36d20, 3' ...   571   e-159  1
(BJ429821) Dictyostelium discoideum cDNA clone:ddv5b02, 3' e...   246   e-146  3
(BJ382681) Dictyostelium discoideum cDNA clone:ddc45e17, 3' ...   494   e-135  1
(AU269806) Dictyostelium discoideum vegetative cDNA clone:VS...   470   e-128  1
(AU269805) Dictyostelium discoideum vegetative cDNA clone:VS...   470   e-128  1
(AU265140) Dictyostelium discoideum vegetative cDNA clone:VS...   186   5e-43  1
(BJ425249) Dictyostelium discoideum cDNA clone:ddv55i05, 5' ...    88   3e-13  1
dna update 2008.11.29
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AL034393_22(AL034393|pid:none) Caenorhabditis elegans YAC Y18D10...    42   0.009
AY093191_1(AY093191|pid:none) Arabidopsis thaliana unknown prote...    40   0.020
AF361833_1(AF361833|pid:none) Arabidopsis thaliana At1g26110/F28...    40   0.020
AC084221_9(AC084221|pid:none) Arabidopsis thaliana chromosome 1 ...    40   0.020
AC079829_24(AC079829|pid:none) Arabidopsis thaliana chromosome 1...    40   0.020
AK317540_1(AK317540|pid:none) Arabidopsis thaliana AT1G26110 mRN...    40   0.033
AM448932_1(AM448932|pid:none) Vitis vinifera contig VV78X114050....    40   0.033
CT005264_55(CT005264|pid:none) Leishmania major strain Friedlin,...    39   0.043
AM910994_689(AM910994|pid:none) Plasmodium knowlesi strain H chr...    39   0.057
AM502243_55(AM502243|pid:none) Leishmania infantum chromosome 25.      38   0.097
protein update 2009. 7.16
PSORT 

 psg: 0.96  gvh: 0.53  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 24.0 %: cytoplasmic
	 16.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VHM121 is mit
5' end seq. ID VHM121F
5' end seq. 
>VHM121F.Seq
ATCAACACCAATACAGTAAATTTAAATAAAGAAATAGATTAAAGTTTTCCATCTATTTAT
TTATTTTCCTCAATTTACAATTTTTCAAATTAATTAATACAATATTAAAGTTAAAAGAAA
AAAAAAANNNNNNNNNN
Length of 5' end seq. 137
3' end seq. ID VHM121Z
3' end seq. 
>VHM121Z.Seq
NNNNNNNNNNACAACCGCCACAGAAATNACAAGCNGCAGTATCTCAAGTAGCATTAGTAG
TACCTNTGGTTTGAATGAGGATGAAATTGACGAGTCTGCAATNGAAGCCATCGCCGCCGG
TATCTCTCATTTACCAACCTACTCACCAACTAGTTTCTTTGATAGCATTTCATGTGAAGC
TTTAGATAAGGCAAACGAAGGTCAAGGTAAATCAAAATCACTNTATGAACAAAGAAAAAT
CGATCAAGAAACCTTTGGTGTTTTATCTGTTAGATATGGTAGAGGTGGTAGAGGTGGAAG
AGGTGGTAGANATAATAATAGTCACAGAC
Length of 3' end seq. 329
Connected seq. ID VHM121P
Connected seq. 
>VHM121P.Seq
ATCAACACCAATACAGTAAATTTAAATAAAGAAATAGATTAAAGTTTTCCATCTATTTAT
TTATTTTCCTCAATTTACAATTTTTCAAATTAATTAATACAATATTAAAGTTAAAAGAAA
AAAAAAA----------ACAACCGCCACAGAAATNACAAGCNGCAGTATCTCAAGTAGCA
TTAGTAGTACCTNTGGTTTGAATGAGGATGAAATTGACGAGTCTGCAATNGAAGCCATCG
CCGCCGGTATCTCTCATTTACCAACCTACTCACCAACTAGTTTCTTTGATAGCATTTCAT
GTGAAGCTTTAGATAAGGCAAACGAAGGTCAAGGTAAATCAAAATCACTNTATGAACAAA
GAAAAATCGATCAAGAAACCTTTGGTGTTTTATCTGTTAGATATGGTAGAGGTGGTAGAG
GTGGAAGAGGTGGTAGANATAATAATAGTCACAGAC
Length of connected seq. 446
Full length Seq ID -
Full length Seq. -
Length of full length seq. -