>VHL819 (VHL819Q) /CSM/VH/VHL8-A/VHL819Q.Seq.d/
XXXXXXXXXXCANATTATACACATGTATTAACTGGTNATAATAACTCTGTTCANGCTTTA
CACACAGTTTTAAAAATTGTAAAAAAATTAAAATCTGAAAATCCAAATTTGATATATGTA
TGTGATCCAGTATTAGGTGATAATAATGAATTATATGTACCAGCNAGATTTAGTTGAAGT
TTATAAGAATGAAGTTATACCAAATGCAGATTATATATTTCCAAATCAAACAGAGGTTGA
ATTTTTAGCAGGTATTANGAATTAAAAATGATCAAGATGCATTAAAAGCCATTGATCAAT
TTCATAAAATGGGTGTGAAAAATGTCGTAATCACAAGTTTATTCTTTGATACAAATCCAA
ATGATATCATTGTTNTTGGTAGTACTATAAACGATGATGATAATAATAATAAATATAATC
AATTTAAAATTACCNGTTGGACCATCAATTTAATGATTATTATACAGGTACTGGCGACTT
GTTAAGTTCANTANTATTAGGTTGGTCT

---xlytcinwx**lcsxfthsfknckkiki*kskfdicm*ssir****iictsxi*lkf
irmklyqmqiiyfqikqrlnf*QVLXIKNDQDALKAIDQFHKMGVKNVVITSLFFDTNPN
DIIVXGSTINDDDNNNKYNQFKITXWTINLMIIIQVLATC*vxxy*vg

Frame A:
---xlytcinwx**lcsxfthsfknckkiki*kskfdicm*ssir****iictsxi*lkf
irmklyqmqiiyfqikqrlnf*QVLXIKNDQDALKAIDQFHKMGVKNVVITSLFFDTNPN
DIIVXGSTINDDDNNNKYNQFKITXWTINLMIIIQVLATC*vxxy*vg


Frame B:
---xythvltgxnnsvxalhtvlkivkklksenpnliyvcdpvlgdnnelyvparfs*sl
*e*sytkcrlyisksnrg*ifsryxelkmikmh*kplinfikwv*kms*sqvysliqiqm
islxlvvl*tmmiiiiniinlklpvgpsi**llyrywrlvkfxxirlv


Frame C:
---iihmy*lviitlfxlytqf*kl*kn*nlkiqi*ymyviqy*viimnymyqxdlvevy
knevipnadyifpnqteveflagixn*k*srciksh*sis*ngcekcrnhkfil*yksk*
yhcxw*yykr******i*si*nyxldhqfndyytgtgdllssxxlgws

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL819 (VHL819Q) /CSM/VH/VHL8-A/VHL819Q.Seq.d/                        757   0.0
VHO636 (VHO636Q) /CSM/VH/VHO6-B/VHO636Q.Seq.d/                         46   5e-04
VHI896 (VHI896Q) /CSM/VH/VHI8-D/VHI896Q.Seq.d/                         46   5e-04
VHA830 (VHA830Q) /CSM/VH/VHA8-B/VHA830Q.Seq.d/                         46   5e-04
FCL-AC26 (FCL-AC26Q) /CSM/FCL/FCL-AC/FCL-AC26Q.Seq.d/                  46   5e-04
AHE479 (AHE479Q) /CSM/AH/AHE4-D/AHE479Q.Seq.d/                         46   5e-04
VHK379 (VHK379Q) /CSM/VH/VHK3-D/VHK379Q.Seq.d/                         42   0.008
SHF854 (SHF854Q) /CSM/SH/SHF8-C/SHF854Q.Seq.d/                         42   0.008
VSK282 (VSK282Q) /CSM/VS/VSK2-D/VSK282Q.Seq.d/                         40   0.032
VSI783 (VSI783Q) /CSM/VS/VSI7-D/VSI783Q.Seq.d/                         40   0.032

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AF136753|AF136753.1 Dictyostelium discoideum pyridoxal kinase gene, complete cds.   276   0.0    5
AE014845|AE014845.1 Plasmodium falciparum 3D7 chromosome 12 section 2 of 9 of the complete sequence.    40   1e-06  13
AL929356|AL929356.1 Plasmodium falciparum strain 3D7, chromosome 9; segment 2/5.    42   1e-05  11
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           46   2e-05  7
AC116551|AC116551.2 Dictyostelium discoideum chromosome 2 map complement(581327-427576) strain AX4, complete sequence.    42   5e-05  8
AC006281|AC006281.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    40   6e-05  11
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    44   6e-05  9
AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                  38   7e-05  9
AC005139|AC005139.3 Plasmodium falciparum chromosome 12, WORKING DRAFT SEQUENCE, 5 unordered pieces.    34   1e-04  11
AC006280|AC006280.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 1 ordered pieces.    48   1e-04  11

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55EK9) RecName: Full=Pyridoxal kinase;          EC=2.7.1.35; A...   118   2e-52
AK066514_1(AK066514|pid:none) Oryza sativa Japonica Group cDNA c...    70   1e-19
CP001326_435(CP001326|pid:none) Micromonas sp. RCC299 chromosome...    73   4e-19
BX294171_4(BX294171|pid:none) Zebrafish DNA sequence from clone ...    63   7e-17
BT082249_1(BT082249|pid:none) Anoplopoma fimbria clone afim-evh-...    61   3e-16
AF400125_1(AF400125|pid:none) Arabidopsis thaliana pyridoxal kin...    70   4e-15
(Q8W1X2) RecName: Full=Pyridoxal kinase;          EC=2.7.1.35; A...    70   4e-15
AE014296_1719(AE014296|pid:none) Drosophila melanogaster chromos...    61   6e-15
BX294175_2(BX294175|pid:none) Zebrafish DNA sequence from clone ...    62   7e-15
BT001545_1(BT001545|pid:none) Drosophila melanogaster RE10625 fu...    61   9e-15

 psg: 0.75  gvh: 0.41  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHL819 is cyt

>VHL819Z.Seq
NNNNNNNNNNCANATTATACACATGTATTAACTGGTNATAATAACTCTGTTCANGCTTTA
CACACAGTTTTAAAAATTGTAAAAAAATTAAAATCTGAAAATCCAAATTTGATATATGTA
TGTGATCCAGTATTAGGTGATAATAATGAATTATATGTACCAGCNAGATTTAGTTGAAGT
TTATAAGAATGAAGTTATACCAAATGCAGATTATATATTTCCAAATCAAACAGAGGTTGA
ATTTTTAGCAGGTATTANGAATTAAAAATGATCAAGATGCATTAAAAGCCATTGATCAAT
TTCATAAAATGGGTGTGAAAAATGTCGTAATCACAAGTTTATTCTTTGATACAAATCCAA
ATGATATCATTGTTNTTGGTAGTACTATAAACGATGATGATAATAATAATAAATATAATC
AATTTAAAATTACCNGTTGGACCATCAATTTAATGATTATTATACAGGTACTGGCGACTT
GTTAAGTTCANTANTATTAGGTTGGTCT