>AFO513 (AFO513Q) /CSM/AF/AFO5-A/AFO513Q.Seq.d/
AAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAGTTATA
TTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATAGATTT
CTTTTTTTTTTTTTTTTTTCCCCXXXXXXXXXXTTGGAATTAAAAATAATGCTCTCATTG
GATGTAAATCGGCTCTTGGTGGTTCTCCTTTTGCCATCTCTAATGCTGTAATACCCATAC
TCCAAATATCAGCTTTTGAATCATATCCAGTTTGTTTAATAACTTCAGGTGCCATCCAAA
ATGGGGTACCAACAAATGTATTTCTCTTTGTCATTTGATCAGTTAATTGACCACTAACAC
CAAAATCAGCTAATTTAACATCGCCGCTTGCTGAAAGTAAAACATTTGCAGCTTTAATAT
CTCTGTGANTTTTACCTTCTGAATGTAAATATTCCAAACCTTTTAATANCTCTCTTG

krtiii*kqshil****qlylni*vkkq*HKGKEKHIIDFFFFFFFP---

---GIKNNALIGCKSALGGSPFAISNAVIPILQISAFESYPVCLITSGAIQNGVPTNVFL
FVI*svn*pltpksanltsplaesktfaalisl*xlpseckyskpfnxsl

Frame A:
ktynyniktithfvivvtvifkyigkktvt*rkrktynrflfffffs---

---lelkimlsldvnrllvvlllpslml*ypyskyqllnhiqfv**lqvpskmgyqqmyf
slsfdqlidh*hqnqli*hrrllkvkhlql*ylcxfyllnvnipnllixll

Frame B:
krtiii*kqshil****qlylni*vkkq*HKGKEKHIIDFFFFFFFP---

---wn*k*cshwm*igswwfsfchl*ccnthtpnisf*iisslfnnfrchpkwgtnkcis
lchlis*lttntkis*fniaac*k*nicsfnisvxftf*m*ifqtf*xls

Frame C:
nvql*yknnhtfcnssnsyi*iyr*knsnikekkni**isffffffp---

---GIKNNALIGCKSALGGSPFAISNAVIPILQISAFESYPVCLITSGAIQNGVPTNVFL
FVI*svn*pltpksanltsplaesktfaalisl*xlpseckyskpfnxsl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO513 (AFO513Q) /CSM/AF/AFO5-A/AFO513Q.Seq.d/                        630   e-180
AFN655 (AFN655Q) /CSM/AF/AFN6-C/AFN655Q.Seq.d/                        620   e-177
SFD665 (SFD665Q) /CSM/SF/SFD6-C/SFD665Q.Seq.d/                        615   e-175
SLG682 (SLG682Q) /CSM/SL/SLG6-D/SLG682Q.Seq.d/                        613   e-174
CFK466 (CFK466Q) /CSM/CF/CFK4-C/CFK466Q.Seq.d/                        611   e-174
SFH469 (SFH469Q) /CSM/SF/SFH4-C/SFH469Q.Seq.d/                        607   e-173
VFH696 (VFH696Q) /CSM/VF/VFH6-D/VFH696Q.Seq.d/                        605   e-172
SFD620 (SFD620Q) /CSM/SF/SFD6-A/SFD620Q.Seq.d/                        591   e-168
AFN101 (AFN101Q) /CSM/AF/AFN1-A/AFN101Q.Seq.d/                        575   e-163
AFI880 (AFI880Q) /CSM/AF/AFI8-D/AFI880Q.Seq.d/                        573   e-163

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF059534|AF059534.1 Dictyostelium discoideum severin kinase mRNA, complete cds.   620  e-174  1
AF061281|AF061281.1 Dictyostelium discoideum kinase responsive to stress 1-like kinase mRNA, complete cds.    66  1e-21  5
AL547396|AL547396.2 human full-length cDNA 5-PRIME end of clone CS0DI007YP04 of PLACENTA COT 25-NORMALIZED of Homo sapiens (human).    62  5e-17  4
BD126332|BD126332.1 Primer for synthesizing full-length cDNA and use thereof.    58  3e-12  4
BD124923|BD124923.1 Primer for synthesizing full-length cDNA and use thereof.    58  3e-12  4
BX369471|BX369471.1 human full-length cDNA 5-PRIME end of clone CS0DJ014YC06 of T CELLS  (JURKAT CELL LINE) COT 10-NORMALIZED of Homo sapiens (human).    58  5e-12  4
BJ073918|BJ073918.1 Xenopus laevis cDNA clone:XL110a13, 5' end, single read.    42  6e-12  4
BD127314|BD127314.1 Primer for synthesizing full-length cDNA and use thereof.    58  7e-12  5
AK074837|AK074837.1 Homo sapiens cDNA FLJ90356 fis, clone NT2RP2003545, weakly similar to Homo sapiens STE20-like kinase 3 (mst-3) mRNA.    58  7e-12  5
CB268648|CB268648.1 1007554 Human Fat Cell 5'-Stretch Plus cDNA Library Homo sapiens cDNA 5', mRNA sequence.    58  7e-12  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O61122) RecName: Full=Serine/threonine-protein kinase svkA;    ...   214   7e-55
BC045867_1(BC045867|pid:none) Danio rerio serine/threonine kinas...   186   2e-46
BT045853_1(BT045853|pid:none) Salmo salar clone ssal-rgf-534-302...   186   2e-46
BX897733_7(BX897733|pid:none) Zebrafish DNA sequence from clone ...   186   2e-46
BC054651_1(BC054651|pid:none) Danio rerio serine/threonine kinas...   186   3e-46
AE017347_297(AE017347|pid:none) Cryptococcus neoformans var. neo...   185   4e-46
AK291808_1(AK291808|pid:none) Homo sapiens cDNA FLJ78718 complet...   185   5e-46
AK303719_1(AK303719|pid:none) Homo sapiens cDNA FLJ61527 complet...   185   5e-46
BC015793_1(BC015793|pid:none) Homo sapiens serine/threonine kina...   185   5e-46
AK148041_1(AK148041|pid:none) Mus musculus B16 F10Y cells cDNA, ...   185   5e-46

 psg: 1.02  gvh: 0.41  alm: 0.37  top: 0.30  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFO513 is cyt

>AFO513F.Seq
AAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAGTTATA
TTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATAGATTT
CTTTTTTTTTTTTTTTTTTCCCC----------

>AFO513Z.Seq
----------TTGGAATTAAAAATAATGCTCTCATTGGATGTAAATCGGCTCTTGGTGGT
TCTCCTTTTGCCATCTCTAATGCTGTAATACCCATACTCCAAATATCAGCTTTTGAATCA
TATCCAGTTTGTTTAATAACTTCAGGTGCCATCCAAAATGGGGTACCAACAAATGTATTT
CTCTTTGTCATTTGATCAGTTAATTGACCACTAACACCAAAATCAGCTAATTTAACATCG
CCGCTTGCTGAAAGTAAAACATTTGCAGCTTTAATATCTCTGTGANTTTTACCTTCTGAA
TGTAAATATTCCAAACCTTTTAATANCTCTCTTG

>AFO513P.Seq
AAAACGTACAATTATAATATAAAAACAATCACACATTTTGTAATAGTAGTAACAGTTATA
TTTAAATATATAGGTAAAAAAACAGTAACATAAAGGAAAAGAAAAACATATAATAGATTT
CTTTTTTTTTTTTTTTTTTCCCC----------TTGGAATTAAAAATAATGCTCTCATTG
GATGTAAATCGGCTCTTGGTGGTTCTCCTTTTGCCATCTCTAATGCTGTAATACCCATAC
TCCAAATATCAGCTTTTGAATCATATCCAGTTTGTTTAATAACTTCAGGTGCCATCCAAA
ATGGGGTACCAACAAATGTATTTCTCTTTGTCATTTGATCAGTTAATTGACCACTAACAC
CAAAATCAGCTAATTTAACATCGCCGCTTGCTGAAAGTAAAACATTTGCAGCTTTAATAT
CTCTGTGANTTTTACCTTCTGAATGTAAATATTCCAAACCTTTTAATANCTCTCTTG