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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO510
Library AF
(Link to library)
Clone ID AFO510
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14919-1
Original site URL
Representative seq. ID AFO510P
(Link to Original site)
Representative DNA sequence 
>AFO510 (AFO510Q) /CSM/AF/AFO5-A/AFO510Q.Seq.d/
ATTATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTT
CAGTATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATA
GAGAAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAA
GAGCAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAG
GTTCAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGA
GAGCATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACA
GTGGTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAA
TGGGTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGA
AGATTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTT
TAATCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATTAATCATTT
CAGGTGCATCTGCTTATCCAAGAGAATGGGATTACAAGAGAATGAGAGCAATCXXXXXXX
XXXATTCACCATTCGATTATTGTGATGTTGTCACCAGTACCACTCATAAAACCTTAAGAG
GACCACGTTCNAGGAATCATCTTCTTTAGAAGAGGTAAAAGAGTCGATGGTAATGGTAAA
GAAATTGAAGAGTACGACATTGAATCAAAGATCAATTTCGCAGTTTTCCCATCACTCCAA
GGTGGTCCACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCTCAAAGAAGCCGACTCT
CAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGCTGCCATTGGTAATGCT
CTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGACAATCATTTAATTCTTTGG
GATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGCTGCCGATATCGCTAAT
ATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGCAATTTCACCAGGTGGTATT
CGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGCCGATTTTGAAAAGATT
GCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCAAGGTCGTGTCGGTAAG
CAAATTGGTTGATTTTGTTGTTGAAATTAATAAAATCAAAAAAAAAAATTAAGTTNANGT
AAATAAG
sequence update 2001. 6. 1
Translated Amino Acid sequence 
yfilltqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSR
AVMEALGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYS
GSPANFAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGL
IDYQRLEENALLFKPKLIISGASAYPREWDYKRMRAI---

---SPFDYCDVVTSTTHKTLRGPRSRNHLL*kr*ksrw*w*rn*rvrh*ikdqfrsfpit
prwst*kcycwccccsqrsrlsriqrvcpss*kecrchw*csheqrl*igykwy*qsfns
lgfktkgthw**i*kscryr*yhrq*kcrpw*yqcnftrwysywficshk*rfkgsrf*k
dc*fp**nrfnls*nsrscr*anwlilllklikskkkikxx*i
Translated Amino Acid sequence (All Frames) 
Frame A:
iilfyshk*lynihihikwvqyqvihh*kklimksln**iekkidnlkv*n*lplkilhq
eqlwkh*dhilqinmqkviqvqdimvvqkllmn*klfvkreh*khsvwmrvngvlmfnht
vvvqpisqftqhy*dhtiv*wv*iyhqvvi*lmvikpirrrfqhhqsfsnqchiklvlmv
*siinv*krmhycsnqn*sfqvhlliqengitre*eq---

---ihhsiivmlspvplikp*edhvxgiiffrrgkrvdgngkeieeydieskinfavfps
lqggphenviagvavalkeadsqefkeyalqvkknaaaignalmnkgyklvtngtdnhli
lwdlrpkeltgnkfekaadianitvnknavhgdtnaispggirigssaltsrglkeadfe
kiadfldrivsisleiqgrvgkqig*fcc*n**nqkkklsxxk*

Frame B:
lfyfthtsnyiiyiyi*ngfsir*ytikrs***nl*ine*rkr*ti*rfrincl*kfyik
ssygsiritfyk*icrrlsrfkilwwyrsc**irnslskesiksipfg*e*mgc*cstiq
w*ssqfrslhsiikttrsyngfrftirwsfnswlsnr*eedfsiinlfrinaisnwc*wf
nrlstfrreciivqtkinhfrciclskrmglqenesn---

---ftirll*cchqyhs*nlkrttfxesssleevkesmvmvkklksttlnqrsisqfshh
skvvhmkmlllvllllskkptlknsksmpfklkrmplplvmls*tkvinwlqmvltii*f
fgi*dqrnslvinlkklpislispsikmpsmvipmqfhqvvfvlvhllsqvev*rkpilk
rllisliesfqsllkfkvvsvsklvdfvveinkikkkn*vxvnk

Frame C:
yfilltqvii*YTYTYKMGSVSGNTPLKEVDNEIFELMNREKDRQFKGLELIASENFTSR
AVMEALGSHFTNKYAEGYPGSRYYGGTEVVDELETLCQKRALKAFRLDESKWGVNVQPYS
GSPANFAVYTALLRPHDRIMGLDLPSGGHLTHGYQTDKKKISASSIFFESMPYQIGADGL
IDYQRLEENALLFKPKLIISGASAYPREWDYKRMRAI---

---SPFDYCDVVTSTTHKTLRGPRSRNHLL*kr*ksrw*w*rn*rvrh*ikdqfrsfpit
prwst*kcycwccccsqrsrlsriqrvcpss*kecrchw*csheqrl*igykwy*qsfns
lgfktkgthw**i*kscryr*yhrq*kcrpw*yqcnftrwysywficshk*rfkgsrf*k
dc*fp**nrfnls*nsrscr*anwlilllklikskkkikxx*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO510 (AFO510Q) /CSM/AF/AFO5-A/AFO510Q.Seq.d/                       2506   0.0
SFI686 (SFI686Q) /CSM/SF/SFI6-D/SFI686Q.Seq.d/                       1294   0.0
VFK411 (VFK411Q) /CSM/VF/VFK4-A/VFK411Q.Seq.d/                       1292   0.0
CFF296 (CFF296Q) /CSM/CF/CFF2-D/CFF296Q.Seq.d/                       1283   0.0
AFA147 (AFA147Q) /CSM/AF/AFA1-B/AFA147Q.Seq.d/                       1281   0.0
SFK139 (SFK139Q) /CSM/SF/SFK1-B/SFK139Q.Seq.d/                       1265   0.0
SFK428 (SFK428Q) /CSM/SF/SFK4-B/SFK428Q.Seq.d/                       1263   0.0
CFC879 (CFC879Q) /CSM/CF/CFC8-D/CFC879Q.Seq.d/                       1245   0.0
AFF146 (AFF146Q) /CSM/AF/AFF1-B/AFF146Q.Seq.d/                       1241   0.0
AFK584 (AFK584Q) /CSM/AF/AFK5-D/AFK584Q.Seq.d/                       1229   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF009966|AF009966.1 Candida albicans serine hydroxymethyl transferase II (SHMII) gene, complete cds.    58  1e-20  6
BI418085|BI418085.1 LjNEST24d6r Lotus japonicus nodule library 5 and 7 week-old Lotus japonicus cDNA 5', mRNA sequence.    60  8e-14  3
BP187516|BP187516.1 Dugesia japonica cDNA, clone: 00331_HN, expressed in the head.    52  3e-12  3
BQ489771|BQ489771.1 14-E9425-006-009-K03-T3 Sugar beet MPIZ-ADIS-006 Lambda Zap II library Beta vulgaris cDNA clone K-3-9, mRNA sequence.    50  4e-12  3
AL409075|AL409075.1 T7 end of clone AV0AA012E03 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    44  4e-12  5
AL408772|AL408772.1 T7 end of clone AV0AA010F02 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    58  4e-11  3
AI960890|AI960890.1 sc92a10.y1 Gm-c1019 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1019-787 5' similar to TR:O04952 O04952 SERINE HYDROXYMETHYLTRANSFERASE ;, mRNA sequence.    52  4e-11  3
BQ091175|BQ091175.1 ku13g11.y1 Strongyloides ratti L2 pAMP1 v1 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:GLYC_CAEEL P50432 SERINE HYDROXYMETHYLTRANSFERASE ;, mRNA sequence.    56  4e-11  3
BQ866049|BQ866049.1 QGC6N09.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC6N09, mRNA sequence.    78  7e-11  2
BG887269|BG887269.1 EST513120 cSTD Solanum tuberosum cDNA clone cSTD4I13 5' sequence, mRNA sequence.    46  4e-10  4
dna update 2003. 9.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BT070546_1(BT070546|pid:none) Picea sitchensis clone WS02735_F19...   315   e-145
CS497866_1(CS497866|pid:none) Sequence 139 from Patent WO2007011...   315   e-143
CS497860_1(CS497860|pid:none) Sequence 133 from Patent WO2007011...   313   e-142
EF150642_1(EF150642|pid:none) Populus tremuloides serine hydroxy...   306   e-142
CS497762_1(CS497762|pid:none) Sequence 35 from Patent WO20070117...   313   e-142
EF150637_1(EF150637|pid:none) Populus tremuloides serine hydroxy...   311   e-142
CS497758_1(CS497758|pid:none) Sequence 31 from Patent WO20070117...   314   e-141
CS497858_1(CS497858|pid:none) Sequence 131 from Patent WO2007011...   308   e-140
CS497862_1(CS497862|pid:none) Sequence 135 from Patent WO2007011...   311   e-140
AF361589_1(AF361589|pid:none) Arabidopsis thaliana AT4g13930/dl3...   305   e-140
protein update 2009. 4.26
PSORT 

 psg: 0.81  gvh: 0.40  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for AFO510 is cyt
5' end seq. ID AFO510F
5' end seq. 
>AFO510F.Seq
ATTATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTT
CAGTATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATA
GAGAAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAA
GAGCAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAG
GTTCAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGA
GAGCATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACA
GTGGTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAA
TGGGTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGA
AGATTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTT
TAATCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATTAATCATTT
CAGGTGCATCTGCTTATCCAAGAGAATGGGATTACAAGAGAATGAGAGCAATC-------
---
Length of 5' end seq. 653
3' end seq. ID AFO510Z
3' end seq. 
>AFO510Z.Seq
----------ATTCACCATTCGATTATTGTGATGTTGTCACCAGTACCACTCATAAAACC
TTAAGAGGACCACGTTCNAGGAATCATCTTCTTTAGAAGAGGTAAAAGAGTCGATGGTAA
TGGTAAAGAAATTGAAGAGTACGACATTGAATCAAAGATCAATTTCGCAGTTTTCCCATC
ACTCCAAGGTGGTCCACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCTCAAAGAAGC
CGACTCTCAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGCTGCCATTGG
TAATGCTCTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGACAATCATTTAAT
TCTTTGGGATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGCTGCCGATAT
CGCTAATATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGCAATTTCACCAGG
TGGTATTCGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGCCGATTTTGA
AAAGATTGCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCAAGGTCGTGT
CGGTAAGCAAATTGGTTGATTTTGTTGTTGAAATTAATAAAATCAAAAAAAAAAATTAAG
TTNANGTAAATAAG
Length of 3' end seq. 664
Connected seq. ID AFO510P
Connected seq. 
>AFO510P.Seq
ATTATTTTATTTTACTCACACAAGTAATTATATAATATACATATACATATAAAATGGGTT
CAGTATCAGGTAATACACCATTAAAAGAAGTTGATAATGAAATCTTTGAATTAATGAATA
GAGAAAAAGATAGACAATTTAAAGGTTTAGAATTAATTGCCTCTGAAAATTTTACATCAA
GAGCAGTTATGGAAGCATTAGGATCACATTTTACAAATAAATATGCAGAAGGTTATCCAG
GTTCAAGATATTATGGTGGTACAGAAGTTGTTGATGAATTAGAAACTCTTTGTCAAAAGA
GAGCATTAAAAGCATTCCGTTTGGATGAGAGTAAATGGGGTGTTAATGTTCAACCATACA
GTGGTAGTCCAGCCAATTTCGCAGTTTACACAGCATTATTAAGACCACACGATCGTATAA
TGGGTTTAGATTTACCATCAGGTGGTCATTTAACTCATGGTTATCAAACCGATAAGAAGA
AGATTTCAGCATCATCAATCTTTTTCGAATCAATGCCATATCAAATTGGTGCTGATGGTT
TAATCGATTATCAACGTTTAGAAGAGAATGCATTATTGTTCAAACCAAAATTAATCATTT
CAGGTGCATCTGCTTATCCAAGAGAATGGGATTACAAGAGAATGAGAGCAATC-------
---ATTCACCATTCGATTATTGTGATGTTGTCACCAGTACCACTCATAAAACCTTAAGAG
GACCACGTTCNAGGAATCATCTTCTTTAGAAGAGGTAAAAGAGTCGATGGTAATGGTAAA
GAAATTGAAGAGTACGACATTGAATCAAAGATCAATTTCGCAGTTTTCCCATCACTCCAA
GGTGGTCCACATGAAAATGTTATTGCTGGTGTTGCTGTTGCTCTCAAAGAAGCCGACTCT
CAAGAATTCAAAGAGTATGCCCTTCAAGTTAAAAAGAATGCCGCTGCCATTGGTAATGCT
CTCATGAACAAAGGTTATAAATTGGTTACAAATGGTACTGACAATCATTTAATTCTTTGG
GATTTAAGACCAAAGGAACTCACTGGTAATAAATTTGAAAAAGCTGCCGATATCGCTAAT
ATCACCGTCAATAAAAATGCCGTCCATGGTGATACCAATGCAATTTCACCAGGTGGTATT
CGTATTGGTTCATCTGCTCTCACAAGTAGAGGTTTAAAGGAAGCCGATTTTGAAAAGATT
GCTGATTTCCTTGATAGAATCGTTTCAATCTCTCTTGAAATTCAAGGTCGTGTCGGTAAG
CAAATTGGTTGATTTTGTTGTTGAAATTAATAAAATCAAAAAAAAAAATTAAGTTNANGT
AAATAAG
Length of connected seq. 1317
Full length Seq ID -
Full length Seq. -
Length of full length seq. -