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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO509
Library AF
(Link to library)
Clone ID AFO509
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15667-1
Original site URL
Representative seq. ID AFO509P
(Link to Original site)
Representative DNA sequence 
>AFO509 (AFO509Q) /CSM/AF/AFO5-A/AFO509Q.Seq.d/
CACTGTTGGCCTACTGGTNAAAAAACTTTATATAATAGTTGATGAAAATAGTGTATTTGA
TTCAACAACTATATCCACAAATAATAATGATAATAATGATAATGATGATAATAATGGTGA
TATTAATGTTAATAATGATAATAATGATAATAATAATAATAATAATAATAATAATAATAA
TAATAATAATAANANNANTAGTNGCNANAACANNNNTTNTNNNATGCATCNGCANCANNA
ACNNNANCANANNNNNNAXXXXXXXXXXTGATGTGATTGACTTCTTGGTGGACTTGACCT
CAAACCATAAGAATGAATAAGAGANCTTTGCAAATGACAATTTCAATCTACGATGATTTC
ATGGACTATATCGTTGGCGATGTTAAACCAGAGAAATTTGAAGAGGTTGATGTCATGCAA
TTGGTGGCTGTCTCTAGTNTAAGTATCGCTTCTAAACTTGAAGAATTGGAAGAATATCAA
CCATCAATTAGGGAATTAAACTATTCAACTAATAATTCCTATACCCATGATGGAATCGTT
AAAACGGAAATGGGAATCTTATCAATTTTAAAATATAAAGTAATGTATCCAACTCCATCT
CATTTCTTGGATTATCTACTTTTAATTACAATTCAACCATACGATTTTTTTGATGCTACC
AAAGACTCCCAAATTACATTTGCAAATCAATTTAAATTAATTAATGACCAAATTTTAAAT
ATTTCTTATAAAAATTTGAAATATAGACAATATTTACCTTCAATTAGTAGCATGTGCATC
AATTGCTTCAACTAGAACTATAATGGGTATTGAACCAGCATGGAAACCATCTTTAAGAAA
A
sequence update 2001. 6. 1
Translated Amino Acid sequence 
TVGLLVKKLYIIVDENSVFDSTTISTNNNDNNDNDDNNGDINVNNDNNDNNNNNNNNNNN
NNNXXXSXXNXXXXMHXXXXXXXXX---

---DVIDFLVDLTSNHKNE*exfandnfnlr*fhglyrwrc*trei*rg*chaiggcl*x
kyrf*t*rigristin*giklfn**flyp*wnr*ngngnlinfki*snvsnsisflglst
fnynstirff*cyqrlpnyicksi*in**pnfkyfl*kfei*tiftfn**hvhqllqlel
*wvlnqhgnhl*e
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgxktlyns**k*ci*fnnyihk***********w*y*c*****************
****xx*xxxxxxxxasaxxtxxxxx---

---*cd*llggldlkp*e*irxlck*qfqstmiswtislamlnqrnlkrlmscnwwlslv
*vsllnlknwkninhqlgn*tiqliipipmmeslkrkwesyqf*nik*ciqlhliswiiy
f*lqfnhtiflmlpktpklhlqinln*lmtkf*ifliki*nidniylqlvacasiastrt
imgiepawkpslrk

Frame B:
TVGLLVKKLYIIVDENSVFDSTTISTNNNDNNDNDDNNGDINVNNDNNDNNNNNNNNNNN
NNNXXXSXXNXXXXMHXXXXXXXXX---

---DVIDFLVDLTSNHKNE*exfandnfnlr*fhglyrwrc*trei*rg*chaiggcl*x
kyrf*t*rigristin*giklfn**flyp*wnr*ngngnlinfki*snvsnsisflglst
fnynstirff*cyqrlpnyicksi*in**pnfkyfl*kfei*tiftfn**hvhqllqlel
*wvlnqhgnhl*e

Frame C:
llaywxknfi**lmkivyliqqlypqiimiimimmiimvilmlimiimiiiiiiiiiiii
iiixxxvaxtxxxxcixxxnxxxxx---

---m*ltswwt*pqtirmnkrxlqmtisiyddfmdyivgdvkpekfeevdvmqlvavssx
siaskleeleeyqpsirelnystnnsythdgivktemgilsilkykvmyptpshfldyll
litiqpydffdatkdsqitfanqfklindqilnisyknlkyrqylpsissmcincfn*ny
ngy*tsmetifkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO509 (AFO509Q) /CSM/AF/AFO5-A/AFO509Q.Seq.d/                       1082   0.0
CHG837 (CHG837Q) /CSM/CH/CHG8-B/CHG837Q.Seq.d/                         46   9e-04
VFM110 (VFM110Q) /CSM/VF/VFM1-A/VFM110Q.Seq.d/                         44   0.003
VFI574 (VFI574Q) /CSM/VF/VFI5-D/VFI574Q.Seq.d/                         44   0.003
SHE511 (SHE511Q) /CSM/SH/SHE5-A/SHE511Q.Seq.d/                         44   0.003
VFN612 (VFN612Q) /CSM/VF/VFN6-A/VFN612Q.Seq.d/                         42   0.014
VFM336 (VFM336Q) /CSM/VF/VFM3-B/VFM336Q.Seq.d/                         42   0.014
VFL630 (VFL630Q) /CSM/VF/VFL6-B/VFL630Q.Seq.d/                         42   0.014
VFI454 (VFI454Q) /CSM/VF/VFI4-C/VFI454Q.Seq.d/                         42   0.014
SHB726 (SHB726Q) /CSM/SH/SHB7-B/SHB726Q.Seq.d/                         42   0.014
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115609|AC115609.1 Dictyostelium discoideum chromosome 2 map 4874582-4895202 strain AX4, *** SEQUENCING IN PROGRESS ***.   666  0.0  6
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.   666  0.0  6
AX458544|AX458544.1 Sequence 90 from Patent WO0246454.            44  0.041  3
BH452345|BH452345.1 BOGQE84TF BOGQ Brassica oleracea genomic clone BOGQE84, DNA sequence.    44  0.071  2
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    34  0.099  5
AC116960|AC116960.2 Dictyostelium discoideum chromosome 2 map complement(1004496-821614) strain AX4, complete sequence.    34  0.14  8
BH964615|BH964615.1 odh57g04.g1 B.oleracea002 Brassica oleracea genomic, DNA sequence.    36  0.22  2
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    38  0.26  5
BH422771|BH422771.1 BOGLM35TF BOGL Brassica oleracea genomic clone BOGLM35, DNA sequence.    46  0.70  1
BZ504752|BZ504752.1 BONMK89TR BO_1.6_2_KB_tot Brassica oleracea genomic clone BONMK89, DNA sequence.    46  0.70  1
dna update 2003. 9.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117081_31(AC117081|pid:none) Dictyostelium discoideum chromoso...   302   2e-91
BC080202_1(BC080202|pid:none) Mus musculus cyclin B1, mRNA (cDNA...    61   4e-08
I48316(I48316) cyclin B1 - mouse  &X64713_1(X64713|pid:none)           61   4e-08
(P30277) RecName: Full=G2/mitotic-specific cyclin-B1;  &BC059113...    61   5e-08
AY618995_1(AY618995|pid:none) Gonyaulax polyedra mitotic cyclin ...    60   8e-08
JH0509(JH0509;S14652) cyclin B - mouse  &X58708_1(X58708|pid:none)     60   1e-07
BC168416_1(BC168416|pid:none) Xenopus tropicalis cyclin J, mRNA ...    53   2e-07
(Q6DFJ9) RecName: Full=Cyclin-J;  &BC076737_1(BC076737|pid:none)       52   4e-07
AK289431_1(AK289431|pid:none) Homo sapiens cDNA FLJ75563 complet...    57   5e-07
AY889745_1(AY889745|pid:none) Synthetic construct Homo sapiens c...    57   5e-07
protein update 2009. 4.26
PSORT 

 psg: 0.64  gvh: 0.27  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial

>> prediction for AFO509 is cyt
5' end seq. ID AFO509F
5' end seq. 
>AFO509F.Seq
CACTGTTGGCCTACTGGTNAAAAAACTTTATATAATAGTTGATGAAAATAGTGTATTTGA
TTCAACAACTATATCCACAAATAATAATGATAATAATGATAATGATGATAATAATGGTGA
TATTAATGTTAATAATGATAATAATGATAATAATAATAATAATAATAATAATAATAATAA
TAATAATAATAANANNANTAGTNGCNANAACANNNNTTNTNNNATGCATCNGCANCANNA
ACNNNANCANANNNNNNA----------
Length of 5' end seq. 258
3' end seq. ID AFO509Z
3' end seq. 
>AFO509Z.Seq
----------TGATGTGATTGACTTCTTGGTGGACTTGACCTCAAACCATAAGAATGAAT
AAGAGANCTTTGCAAATGACAATTTCAATCTACGATGATTTCATGGACTATATCGTTGGC
GATGTTAAACCAGAGAAATTTGAAGAGGTTGATGTCATGCAATTGGTGGCTGTCTCTAGT
NTAAGTATCGCTTCTAAACTTGAAGAATTGGAAGAATATCAACCATCAATTAGGGAATTA
AACTATTCAACTAATAATTCCTATACCCATGATGGAATCGTTAAAACGGAAATGGGAATC
TTATCAATTTTAAAATATAAAGTAATGTATCCAACTCCATCTCATTTCTTGGATTATCTA
CTTTTAATTACAATTCAACCATACGATTTTTTTGATGCTACCAAAGACTCCCAAATTACA
TTTGCAAATCAATTTAAATTAATTAATGACCAAATTTTAAATATTTCTTATAAAAATTTG
AAATATAGACAATATTTACCTTCAATTAGTAGCATGTGCATCAATTGCTTCAACTAGAAC
TATAATGGGTATTGAACCAGCATGGAAACCATCTTTAAGAAAA
Length of 3' end seq. 573
Connected seq. ID AFO509P
Connected seq. 
>AFO509P.Seq
CACTGTTGGCCTACTGGTNAAAAAACTTTATATAATAGTTGATGAAAATAGTGTATTTGA
TTCAACAACTATATCCACAAATAATAATGATAATAATGATAATGATGATAATAATGGTGA
TATTAATGTTAATAATGATAATAATGATAATAATAATAATAATAATAATAATAATAATAA
TAATAATAATAANANNANTAGTNGCNANAACANNNNTTNTNNNATGCATCNGCANCANNA
ACNNNANCANANNNNNNA----------TGATGTGATTGACTTCTTGGTGGACTTGACCT
CAAACCATAAGAATGAATAAGAGANCTTTGCAAATGACAATTTCAATCTACGATGATTTC
ATGGACTATATCGTTGGCGATGTTAAACCAGAGAAATTTGAAGAGGTTGATGTCATGCAA
TTGGTGGCTGTCTCTAGTNTAAGTATCGCTTCTAAACTTGAAGAATTGGAAGAATATCAA
CCATCAATTAGGGAATTAAACTATTCAACTAATAATTCCTATACCCATGATGGAATCGTT
AAAACGGAAATGGGAATCTTATCAATTTTAAAATATAAAGTAATGTATCCAACTCCATCT
CATTTCTTGGATTATCTACTTTTAATTACAATTCAACCATACGATTTTTTTGATGCTACC
AAAGACTCCCAAATTACATTTGCAAATCAATTTAAATTAATTAATGACCAAATTTTAAAT
ATTTCTTATAAAAATTTGAAATATAGACAATATTTACCTTCAATTAGTAGCATGTGCATC
AATTGCTTCAACTAGAACTATAATGGGTATTGAACCAGCATGGAAACCATCTTTAAGAAA
A
Length of connected seq. 831
Full length Seq ID -
Full length Seq. -
Length of full length seq. -