>VHL423 (VHL423Q) /CSM/VH/VHL4-A/VHL423Q.Seq.d/
XXXXXXXXXXTGGATTTTTCATCGCTGGTGTTGCCTCCAAAGTACTCATGATGCAATGTA
CCTTTTGTATCAACTCTTTGGCTCATTATCTTGGTGAAGCAACTTACACCGACCAAAGAT
CTCCACGTGACTCTTTTATCACATCTTTGGTCACTTTTGGTGAAGGTTATCATAACTTTC
ATCATGAATTCCCATATGATTACAGAAATGGTATTCATATGTCTGCTTACGATCCAGGTA
AGTGGTTAATCAATTTCCTTAGTTGGNTTGGTTTCGCCTATGAATTAAAGAGATTCCCAA
GTGAATTATTCGCAAAAGGTAAAATTCAAATGCAAGAGACAAACCATCAAAAAATTACGT
GATCAACTCTATTGGGGTAANCCATTGGATCAATTACCATCATTCACTCGTGCTCNAGTT
AAAGAGAAATGTCAAAAAGAAAAGAAACAACTCTTAATCATTGAAGATGTTATNTATGAT
AT

---GFFIAGVASKVLMMQCTFCINSLAHYLGEATYTDQRSPRDSFITSLVTFGEGYHNFH
HEFPYDYRNGIHMSAYDPGKWLINFLSWXGFAYELKRFPSELFAKGKIQMQETNHQKIT*
stllg*xigsitiihscss*remskrkettlnh*rcyx*y

Frame A:
---GFFIAGVASKVLMMQCTFCINSLAHYLGEATYTDQRSPRDSFITSLVTFGEGYHNFH
HEFPYDYRNGIHMSAYDPGKWLINFLSWXGFAYELKRFPSELFAKGKIQMQETNHQKIT*
stllg*xigsitiihscss*remskrkettlnh*rcyx*y


Frame B:
---dfsslvlppkys*cnvpfvstlwliilvkqltptkdlhvtllshlwsllvkviitfi
mnshmitemvficlltiqvsg*sislvglvspmn*rdsqvnysqkvkfkckrqtikklrd
qlywgxpldqlpsftraxvkekcqkekkqlliiedvxyd


Frame C:
---ifhrwcclqsthdamyllyqlfgslsw*snlhrpkist*lfyhifghfw*rls*lss
*ipi*lqkwysyvclrsr*vvnqfp*lxwfrl*ikeipk*iirkr*nsnardkpsknyvi
nsigvxhwinyhhslvlxlkrnvkkkrnns*slkmlxmi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL423 (VHL423Q) /CSM/VH/VHL4-A/VHL423Q.Seq.d/                        912   0.0
VHQ471 (VHQ471Q) /CSM/VH/VHQ4-C/VHQ471Q.Seq.d/                        896   0.0
VHP830 (VHP830Q) /CSM/VH/VHP8-B/VHP830Q.Seq.d/                        896   0.0
VHP116 (VHP116Q) /CSM/VH/VHP1-A/VHP116Q.Seq.d/                        896   0.0
VHO360 (VHO360Q) /CSM/VH/VHO3-C/VHO360Q.Seq.d/                        896   0.0
VHM788 (VHM788Q) /CSM/VH/VHM7-D/VHM788Q.Seq.d/                        896   0.0
VHM540 (VHM540Q) /CSM/VH/VHM5-B/VHM540Q.Seq.d/                        896   0.0
VHL784 (VHL784Q) /CSM/VH/VHL7-D/VHL784Q.Seq.d/                        896   0.0
VHL116 (VHL116Q) /CSM/VH/VHL1-A/VHL116Q.Seq.d/                        896   0.0
VHK569 (VHK569Q) /CSM/VH/VHK5-C/VHK569Q.Seq.d/                        896   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    74   2e-09  1
AL033501|AL033501.1 C.albicans cosmid Ca41C10.                     70   3e-08  1
AQ874658|AQ874658.1 V112D8 mTn-3xHA/lacZ Insertion Library, strain Y2278 Saccharomyces cerevisiae genomic 5', DNA sequence.    56   5e-08  2
AR016873|AR016873.1 Sequence 1 from patent US 5777201.             56   2e-07  2
Z72577|Z72577.1 S.cerevisiae chromosome VII reading frame ORF YGL055w.    56   5e-07  2
AL396412|AL396412.1 T3 end of clone AR0AA030B05 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    56   5e-04  1
J05676|J05676.1 Saccharomyces cerevisiae delta-9 fatty acid desaturase (OLE1) gene, complete cds.    56   5e-04  1
AB024576|AB024576.1 Pichia angusta H-OLE1 gene for delta9-fatty acid desaturase, complete cds.    54   0.002  1
BM027442|BM027442.1 GIT0000801 Root-induced cDNA library from Glomus intraradices Glomus intraradices cDNA, mRNA sequence.    54   0.002  1
CR318630|CR318630.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-225K7.    40   0.030  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY995173_1(AY995173|pid:none) Amylomyces rouxii delta-9 desatura...   147   2e-34
AB196774_1(AB196774|pid:none) Mortierella alpina gene for omega9...   136   2e-31
AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   131   7e-30
CR382123_238(CR382123|pid:none) Kluyveromyces lactis strain NRRL...   128   9e-30
FJ695614_1(FJ695614|pid:none) Piriformospora indica delta 9-fatt...   131   9e-30
AB071696_2(AB071696|pid:none) Saccharomyces kluyveri Sk-SDS23 ge...   126   1e-29
DQ901954_1(DQ901954|pid:none) Aureobasidium pullulans putative d...   125   2e-29
D83185_1(D83185|pid:none) Pichia angusta DNA for delta 9-fatty a...   128   2e-29
CU928175_208(CU928175|pid:none) Zygosaccharomyces rouxii strain ...   129   3e-29
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   129   5e-29

 psg: 0.91  gvh: 0.66  alm: 0.54  top: 0.43  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 24.0 %: nuclear
	  8.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for VHL423 is exc

>VHL423Z.Seq
NNNNNNNNNNTGGATTTTTCATCGCTGGTGTTGCCTCCAAAGTACTCATGATGCAATGTA
CCTTTTGTATCAACTCTTTGGCTCATTATCTTGGTGAAGCAACTTACACCGACCAAAGAT
CTCCACGTGACTCTTTTATCACATCTTTGGTCACTTTTGGTGAAGGTTATCATAACTTTC
ATCATGAATTCCCATATGATTACAGAAATGGTATTCATATGTCTGCTTACGATCCAGGTA
AGTGGTTAATCAATTTCCTTAGTTGGNTTGGTTTCGCCTATGAATTAAAGAGATTCCCAA
GTGAATTATTCGCAAAAGGTAAAATTCAAATGCAAGAGACAAACCATCAAAAAATTACGT
GATCAACTCTATTGGGGTAANCCATTGGATCAATTACCATCATTCACTCGTGCTCNAGTT
AAAGAGAAATGTCAAAAAGAAAAGAAACAACTCTTAATCATTGAAGATGTTATNTATGAT
AT