>VHL343 (VHL343Q) /CSM/VH/VHL3-B/VHL343Q.Seq.d/
XXXXXXXXXXAGTCGATAGTGGTGTCATCAGTTTGGATGAAAATAAATGGATTGCAATTG
GTTCAAAGATTTTCACCTTTGTCAGTGAAACCATCAAAGCTTTGGCCGATATCAAATTGG
CCGATNTTAGCTTTAGATTATACCTTCAAGCACTCCAAACCTTTGACCACTGTGGTTTGG
TCTCTCGTGTCAAAGAATTGGCCATCAAGGCTCTCTTAATCTTCCAAGAAGATATCGCCG
ATTTCAAAGCTCAAGTTATGGCCCTTCAATTGTTAATCTCTACTCTCAATAGTTTAAGCA
TTCCAAATGAAGAGATCTATGAATCCTTGGCCGCCCAAACCATTAAACAAGCTTCTCGTC
TTCTCTTACCACAAGATCAAGCAAAACTCATCTCTACTTGTTCACATTTATTCTGGGTTG
ATAATCCTTCTCGTCAATATCAAAATCCAGACTCTGTATTACAAGCTCTCAAAAAAGCTC
TCTCCATTATCAGTAATGAATCGAGTCCTGGTCTTGGTACATCTTGTTGGCATTTTGAAT
GAATGTCTCTTCTATTGTGATAAAGAAACTGATGCTGTTCCAATTCAATTCGTTTCAGAT
TAGGTNGAATTAATTCGTACTACTCATGTTAAAGAAGCNATCCAGCATTACCATATTTAC
AAAATACTATCAAATACATTCAATCTCAAAA

---VDSGVISLDENKWIAIGSKIFTFVSETIKALADIKLADXSFRLYLQALQTFDHCGLV
SRVKELAIKALLIFQEDIADFKAQVMALQLLISTLNSLSIPNEEIYESLAAQTIKQASRL
LLPQDQAKLISTCSHLFWVDNPSRQYQNPDSVLQALKKALSIISNESSPGLGTSCWHFE*
mslll**rn*ccsnsirfrlgxinsyysc*rsxpalpylqntikyiqsq

Frame A:
---VDSGVISLDENKWIAIGSKIFTFVSETIKALADIKLADXSFRLYLQALQTFDHCGLV
SRVKELAIKALLIFQEDIADFKAQVMALQLLISTLNSLSIPNEEIYESLAAQTIKQASRL
LLPQDQAKLISTCSHLFWVDNPSRQYQNPDSVLQALKKALSIISNESSPGLGTSCWHFE*
mslll**rn*ccsnsirfrlgxinsyysc*rsxpalpylqntikyiqsq


Frame B:
---sivvssvwmkinglqlvqrfsplsvkpsklwpisnwpxlaldytfkhskplttvvws
lvsknwpsrls*sskkispisklklwpfnc*sllsiv*afqmkrsmnpwppkplnkllvf
syhkikqnssllvhiysgliillvnikiqtlyyklskklsplsvmnrvlvlvhlvgilne
clfycdketdavpiqfvsd*velirtthvkeaiqhyhiykilsntfnlk


Frame C:
---r*wchqfg*k*mdcnwfkdfhlcq*nhqsfgryqigrx*l*iipsstpnl*plwfgl
scqrighqgslnlprryrrfqsssygpsivnlysq*fkhsk*rdl*ilgrpnh*tsfsss
lttrsskthlylftfilg**sfssisksrlcitssqksslhyq**ieswswyillaf*mn
vssivikklmlfqfnsfqirxn*fvllmlkkxssitiftkyyqihsisk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL343 (VHL343Q) /CSM/VH/VHL3-B/VHL343Q.Seq.d/                       1332   0.0
VHM748 (VHM748Q) /CSM/VH/VHM7-B/VHM748Q.Seq.d/                       1296   0.0
AHJ343 (AHJ343Q) /CSM/AH/AHJ3-B/AHJ343Q.Seq.d/                       1291   0.0
VHK662 (VHK662Q) /CSM/VH/VHK6-C/VHK662Q.Seq.d/                       1287   0.0
VHI583 (VHI583Q) /CSM/VH/VHI5-D/VHI583Q.Seq.d/                       1287   0.0
VHH532 (VHH532Q) /CSM/VH/VHH5-B/VHH532Q.Seq.d/                       1287   0.0
CFF491 (CFF491Q) /CSM/CF/CFF4-D/CFF491Q.Seq.d/                       1287   0.0
AHH565 (AHH565Q) /CSM/AH/AHH5-C/AHH565Q.Seq.d/                       1287   0.0
AHA519 (AHA519Q) /CSM/AH/AHA5-A/AHA519Q.Seq.d/                       1287   0.0
VHI863 (VHI863Q) /CSM/VH/VHI8-C/VHI863Q.Seq.d/                       1279   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL050350|AL050350.14 Human DNA sequence from clone RP1-261K5 on chromosome 6q21-22.1.    46   0.67   1
AQ304508|AQ304508.1 HS_2209_A2_F03_MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2209 Col=6 Row=K, genomic survey sequence.    46   0.67   1
BE579076|BE579076.1 kq22a02.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to SW:MEM2_MOUSE P28660 MEMBRANE-ASSOCIATED PROTEIN HEM-2 ;, mRNA sequence.    46   0.67   1
AL031674|AL031674.1 Human DNA sequence from clone RP4-715N11 on chromosome 20q13.1-13.2 Contains two putative novel genes, ESTs, STSs and GSSs.    32   2.1    7
AC139651|AC139651.2 Rattus norvegicus clone CH230-165I3, WORKING DRAFT SEQUENCE, 46 unordered pieces.    36   2.3    5
AP003238|AP003238.4 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, PAC clone:P0401G10.    44   2.6    1
AK066497|AK066497.1 Oryza sativa (japonica cultivar-group) cDNA clone:J013071N18, full insert sequence.    44   2.6    1
AC087518|AC087518.5 Homo sapiens chromosome , clone RP11-359E19, complete sequence.    34   4.0    6
AY123429|AY123429.1 Papio anubis anubis neurofibromatosis type 2 region, section 4; and merlin (NF2) gene, exons 2 through 16 and complete cds.    34   4.0    3
AC133850|AC133850.1 Rattus norvegicus clone CH230-6N10, *** SEQUENCING IN PROGRESS ***, 35 unordered pieces.    34   5.5    3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54C24) RecName: Full=Vacuolar sorting protein 35;                   338   1e-91
AF175265_1(AF175265|pid:none) Homo sapiens vacuolar sorting prot...    94   1e-20
AK223218_1(AK223218|pid:none) Homo sapiens mRNA for vacuolar pro...    94   1e-20
(Q9EQH3) RecName: Full=Vacuolar protein sorting-associated prote...    94   1e-20
AK166821_1(AK166821|pid:none) Mus musculus blastocyst blastocyst...    94   1e-20
CR926124_1(CR926124|pid:none) Pongo abelii mRNA; cDNA DKFZp459E1...    94   1e-20
AK023650_1(AK023650|pid:none) Homo sapiens cDNA FLJ13588 fis, cl...    94   1e-20
AL512769_1(AL512769|pid:none) Homo sapiens mRNA; cDNA DKFZp434E1...    94   1e-20
BC169004_1(BC169004|pid:none) Rattus norvegicus vacuolar protein...    94   2e-20
AB169708_1(AB169708|pid:none) Macaca fascicularis brain cDNA, cl...    93   3e-20

 psg: 0.75  gvh: 0.47  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for VHL343 is nuc

>VHL343Z.Seq
NNNNNNNNNNAGTCGATAGTGGTGTCATCAGTTTGGATGAAAATAAATGGATTGCAATTG
GTTCAAAGATTTTCACCTTTGTCAGTGAAACCATCAAAGCTTTGGCCGATATCAAATTGG
CCGATNTTAGCTTTAGATTATACCTTCAAGCACTCCAAACCTTTGACCACTGTGGTTTGG
TCTCTCGTGTCAAAGAATTGGCCATCAAGGCTCTCTTAATCTTCCAAGAAGATATCGCCG
ATTTCAAAGCTCAAGTTATGGCCCTTCAATTGTTAATCTCTACTCTCAATAGTTTAAGCA
TTCCAAATGAAGAGATCTATGAATCCTTGGCCGCCCAAACCATTAAACAAGCTTCTCGTC
TTCTCTTACCACAAGATCAAGCAAAACTCATCTCTACTTGTTCACATTTATTCTGGGTTG
ATAATCCTTCTCGTCAATATCAAAATCCAGACTCTGTATTACAAGCTCTCAAAAAAGCTC
TCTCCATTATCAGTAATGAATCGAGTCCTGGTCTTGGTACATCTTGTTGGCATTTTGAAT
GAATGTCTCTTCTATTGTGATAAAGAAACTGATGCTGTTCCAATTCAATTCGTTTCAGAT
TAGGTNGAATTAATTCGTACTACTCATGTTAAAGAAGCNATCCAGCATTACCATATTTAC
AAAATACTATCAAATACATTCAATCTCAAAA