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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL332
Library VH
(Link to library)
Clone ID VHL332
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHL332P
(Link to Original site)
Representative DNA sequence 
>VHL332 (VHL332Q) /CSM/VH/VHL3-B/VHL332Q.Seq.d/
CACTGTTGGCCTACTGGTAATATGCATTATTGTTAAAATAAGAGTAAATATTAAATAATT
AAATATTAAAAAATGTCATCAATTGGTATTGATTTAGGTACAACATATTTATGTGTTGGT
GTTTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCA
TCTTATGTTGCATTTACTGATACGGAACGTTNAATTGGTGATGCTGCAAAGAATCAAGTA
GCAATGAATCCXXXXXXXXXXAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCA
AGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAA
GAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTC
TATCAAATAGATTTCTAAAAAANA
sequence update 2002.10.25
Translated Amino Acid sequence 
hcwptgnmhyc*nksky*iiky*KMSSIGIDLGTTYLCVGVWQNDRVEIIANDQGNRTTP
SYVAFTDTERXIGDAAKNQVAMN---

---EAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*i
skk
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgnmhyc*nksky*iiky*KMSSIGIDLGTTYLCVGVWQNDRVEIIANDQGNRTTP
SYVAFTDTERXIGDAAKNQVAMN---

---rsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqid
f*kx

Frame B:
tvgllviciivkirvnik*lnikkchqlvli*vqhiyvlvfgkmivlkllqmikvieplh
lmlhllirnvxlvmlqrik*q*i---

---EAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*i
skk

Frame C:
llayw*yalllk*e*ilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsi
lcciy*ygtxnw*cckesssnes---

---kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrf
lkx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CHJ874 (CHJ874Q) /CSM/CH/CHJ8-D/CHJ874Q.Seq.d/                        347   9e-95
VSH706 (VSH706Q) /CSM/VS/VSH7-A/VSH706Q.Seq.d/                        347   9e-95
VSH453 (VSH453Q) /CSM/VS/VSH4-C/VSH453Q.Seq.d/                        347   9e-95
VSF403 (VSF403Q) /CSM/VS/VSF4-A/VSF403Q.Seq.d/                        347   9e-95
VSF348 (VSF348Q) /CSM/VS/VSF3-B/VSF348Q.Seq.d/                        347   9e-95
VSA155 (VSA155Q) /CSM/VS/VSA1-C/VSA155Q.Seq.d/                        347   9e-95
VHQ266 (VHQ266Q) /CSM/VH/VHQ2-C/VHQ266Q.Seq.d/                        347   9e-95
VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                        347   9e-95
VHQ240 (VHQ240Q) /CSM/VH/VHQ2-B/VHQ240Q.Seq.d/                        347   9e-95
VHP873 (VHP873Q) /CSM/VH/VHP8-D/VHP873Q.Seq.d/                        347   9e-95
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X75263) D.discoideum (AX3) mRNA for heat shock cognate prot...   351   0.0    2
(L22736) Dictyostelium discoideum heat shock protein (hspB) ...   351   e-131  2
(BJ424751) Dictyostelium discoideum cDNA clone:ddv53p07, 5' ...   345   e-102  2
(BJ384540) Dictyostelium discoideum cDNA clone:ddc49h19, 3' ...   359   1e-95  2
(BJ443597) Dictyostelium discoideum cDNA clone:ddv53p07, 3' ...   359   5e-95  1
(C92590) Dictyostelium discoideum slug cDNA, clone SSF159.        351   2e-93  2
(AU034580) Dictyostelium discoideum slug cDNA, clone SLC515.      351   2e-93  2
(BJ436388) Dictyostelium discoideum cDNA clone:ddv31c05, 3' ...   351   2e-93  2
(AU039577) Dictyostelium discoideum slug cDNA, clone SLE781.      351   2e-93  2
(BJ399004) Dictyostelium discoideum cDNA clone:dds3g16, 3' e...   351   2e-93  2
dna update 2008.11.26
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P25840) RecName: Full=Heat shock 70 kDa protein;  &FB790406_1(F...   114   1e-24
(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   114   1e-24
AF188289_1(AF188289|pid:none) Rhizopus stolonifer Hsp70 protein ...   114   1e-24
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   114   1e-24
(Q01877) RecName: Full=Heat shock protein HSS1;  &U26597_1(U2659...   113   2e-24
M26595_1(M26595|pid:none) T.cruzi heat shock protein (HSP70) gen...   112   3e-24
(P41753) RecName: Full=Heat shock 70 kDa protein;  &A53163(A5316...   112   3e-24
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   112   3e-24
(Q07437) RecName: Full=Heat shock 70 kDa protein;  &L14604_1(L14...   112   3e-24
FJ168549_2(FJ168549|pid:none) Bodo saltans clone fosmid 16K02, c...   112   3e-24
protein update 2009. 7.16
PSORT 

 psg: 0.89  gvh: 0.38  alm: 0.42  top: 0.27  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHL332 is cyt
5' end seq. ID VHL332F
5' end seq. 
>VHL332F.Seq
CACTGTTGGCCTACTGGTAATATGCATTATTGTTAAAATAAGAGTAAATATTAAATAATT
AAATATTAAAAAATGTCATCAATTGGTATTGATTTAGGTACAACATATTTATGTGTTGGT
GTTTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCA
TCTTATGTTGCATTTACTGATACGGAACGTTNAATTGGTGATGCTGCAAAGAATCAAGTA
GCAATGAATCCNNNNNNNNNN
Length of 5' end seq. 261
3' end seq. ID VHL332Z
3' end seq. 
>VHL332Z.Seq
NNNNNNNNNNAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTA
TGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACA
ACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGA
TTTCTAAAAAANA
Length of 3' end seq. 193
Connected seq. ID VHL332P
Connected seq. 
>VHL332P.Seq
CACTGTTGGCCTACTGGTAATATGCATTATTGTTAAAATAAGAGTAAATATTAAATAATT
AAATATTAAAAAATGTCATCAATTGGTATTGATTTAGGTACAACATATTTATGTGTTGGT
GTTTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCA
TCTTATGTTGCATTTACTGATACGGAACGTTNAATTGGTGATGCTGCAAAGAATCAAGTA
GCAATGAATCC----------AGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCA
AGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAA
GAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTC
TATCAAATAGATTTCTAAAAAANA
Length of connected seq. 434
Full length Seq ID -
Full length Seq. -
Length of full length seq. -