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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO474
Library AF
(Link to library)
Clone ID AFO474
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFO474Z
(Link to Original site)
Representative DNA sequence 
>AFO474 (AFO474Q) /CSM/AF/AFO4-D/AFO474Q.Seq.d/
XXXXXXXXXXGTATGAATCAATCGCCGTTCCATTAGCTTATTTATTCATTGACTCCCACC
TCAAGATAACACTCAAGTTCTCGAAGATGTAAAGAAAATCGCTACCTCACAAAAAAGGTA
ATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTC
TCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACA
ATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCA
TTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTC
CAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATG
TTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACG
ATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCG
ACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCA
AAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACT
TTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTG
ATGATAATGTNAATCTAAAAAAGATTCTTCTGCTAAACANANAATTATAAATAATTAATA
AATATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---yesiavplaylfidshlkitlkfskm*RKSLPHKKGNAVFCWVDMKKFPQQATHMGL
SGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAP
VKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDAD
SNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTD
DNVNLKKILLLNXXL*iinky
Translated Amino Acid sequence (All Frames) 
Frame A:
---yesiavplaylfidshlkitlkfskm*RKSLPHKKGNAVFCWVDMKKFPQQATHMGL
SGKVVPAISVDSVANKARYNFDEKETFSFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAP
VKVAVGTTFKKLVLDSPKDVLVEFYAPWCGHCKNLAPIYDKLGEYLKDVESVSIVKIDAD
SNDVPSDIEIRGYPTIMLFKADDKENPISYEGQRNDHMNFVEFIQDNAAIEFKLPSSQTD
DNVNLKKILLLNXXL*iinky


Frame B:
---mnqspfh*liysltptsr*hsssrrckenryltkkvmpfsvgsi*rnshnkqliwvs
lvklfqpslliqlpiklvtismkkkpshsipspngskmslvvkflhllnlnqnqnqmmlq
lrlplvplskswf*thqrmfwlnstlhgaviarillqstin*vnt*ktlnlsqs*rlmpt
qmmyqvisklevtqpscssklmtkkiqslmkvkemii*tllnsfkitlplnsnyhhlklm
imxi*krffc*txnyk*lini


Frame C:
---*inrrsislfih*lppqdntqvledvkkiatsqkr*crfllgryeeipttsnsygsl
w*scsshlc*fscq*sslqfr*krnlliryrlqmdprchww*sfsic*istrtrik*css
*gcrwyhfqkvgfrltkgcfg*ilrsmvrslqescsnlr*ir*ilkrr*iclnrkd*crl
k*ctk*yrn*rlpnhhalqs**qrksnll*rskk*syelc*ihsr*rch*iqitiisn**
*cxskkdssakxxiinn**i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO474 (AFO474Q) /CSM/AF/AFO4-D/AFO474Q.Seq.d/                       1520   0.0
AFN855 (AFN855Q) /CSM/AF/AFN8-C/AFN855Q.Seq.d/                       1443   0.0
AFL563 (AFL563Q) /CSM/AF/AFL5-C/AFL563Q.Seq.d/                       1437   0.0
AFM464 (AFM464Q) /CSM/AF/AFM4-C/AFM464Q.Seq.d/                       1411   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1411   0.0
AFK889 (AFK889Q) /CSM/AF/AFK8-D/AFK889Q.Seq.d/                       1407   0.0
AFO630 (AFO630Q) /CSM/AF/AFO6-B/AFO630Q.Seq.d/                       1405   0.0
AFO368 (AFO368Q) /CSM/AF/AFO3-C/AFO368Q.Seq.d/                       1398   0.0
AFN679 (AFN679Q) /CSM/AF/AFN6-D/AFN679Q.Seq.d/                       1394   0.0
AFO686 (AFO686Q) /CSM/AF/AFO6-D/AFO686Q.Seq.d/                       1390   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  3e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  5e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  5e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  2e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  3e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  1e-05  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  4e-05  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  4e-05  1
BM174369|PDA4_MOUSE PROTEIN DISULFIDE ISOMERASE A4 PRECURSOR (PROTEIN ERP-72) (ERP72), mRNA sequence.    60  4e-05  1
dna update 2003. 9.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   172   1e-41
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   169   9e-41
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   169   9e-41
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   163   5e-39
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   161   2e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   159   9e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   154   2e-36
AB022013_1(AB022013|pid:none) Antheraea pernyi mRNA for protein ...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   3e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   3e-35
protein update 2009. 4.26
PSORT 

 psg: 0.86  gvh: 0.36  alm: 0.43  top: 0.43  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for AFO474 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFO474Z
3' end seq. 
>AFO474Z.Seq
----------GTATGAATCAATCGCCGTTCCATTAGCTTATTTATTCATTGACTCCCACC
TCAAGATAACACTCAAGTTCTCGAAGATGTAAAGAAAATCGCTACCTCACAAAAAAGGTA
ATGCCGTTTTCTGTTGGGTCGATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTC
TCTCTGGTAAAGTTGTTCCAGCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACA
ATTTCGATGAAAAAGAAACCTTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCA
TTGGTGGTAAAGTTTCTCCATTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTC
CAGTTAAGGTTGCCGTTGGTACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATG
TTTTGGTTGAATTCTACGCTCCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACG
ATAAATTAGGTGAATACTTAAAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCG
ACTCAAATGATGTACCAAGTGATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCA
AAGCTGATGACAAAGAAAATCCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACT
TTGTTGAATTCATTCAAGATAACGCTGCCATTGAATTCAAATTACCATCATCTCAAACTG
ATGATAATGTNAATCTAAAAAAGATTCTTCTGCTAAACANANAATTATAAATAATTAATA
AATATA
Length of 3' end seq. 776
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -