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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL190
Library VH
(Link to library)
Clone ID VHL190
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHL190P
(Link to Original site)
Representative DNA sequence 
>VHL190 (VHL190Q) /CSM/VH/VHL1-D/VHL190Q.Seq.d/
ATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAG
CTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAA
ATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAXXXXXXX
XXXTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACC
AAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGT
GTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCT
GAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTT
TCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAA
CAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAA
AATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATT
GAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGAT
GAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTC
TATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCA
CCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAAC
ATCTCTATCAAATAGATTTCTTAAAAAATATAGTAGTAAATCAATAAAGCTTCTCAT
sequence update 2002.10.25
Translated Amino Acid sequence 
c*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTER
LIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFK
GETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLN
VQRIINEPTAAAIAYGL---

---SDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNV
SAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFT
VKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMS
KLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*ki***inkas
h
Translated Amino Acid sequence (All Frames) 
Frame A:
ivkirvnik*lnikkchqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlliqn
v*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifklns
kvklkfshqkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*
mfnvllmnqllllllmv---

---SDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNV
SAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFT
VKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMS
KLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*ki***inkas
h

Frame B:
llk*e*ilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*yrt
fnw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*iq
r*n*sfltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkik
cstyy**tncccyclwf---

---liinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*my
qlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsl
lkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*v
nsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkkyssksikll

Frame C:
c*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTER
LIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFK
GETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLN
VQRIINEPTAAAIAYGL---

---**strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkci
s*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc
*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*
tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknivvnq*sfs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL190 (VHL190Q) /CSM/VH/VHL1-D/VHL190Q.Seq.d/                       2460   0.0
VHL319 (VHL319Q) /CSM/VH/VHL3-A/VHL319Q.Seq.d/                       2438   0.0
CHO304 (CHO304Q) /CSM/CH/CHO3-A/CHO304Q.Seq.d/                       1413   0.0
AHI810 (AHI810Q) /CSM/AH/AHI8-A/AHI810Q.Seq.d/                       1413   0.0
CHN526 (CHN526Q) /CSM/CH/CHN5-B/CHN526Q.Seq.d/                       1411   0.0
AHI583 (AHI583Q) /CSM/AH/AHI5-D/AHI583Q.Seq.d/                       1411   0.0
SFL461 (SFL461Q) /CSM/SF/SFL4-C/SFL461Q.Seq.d/                       1409   0.0
VHM303 (VHM303Q) /CSM/VH/VHM3-A/VHM303Q.Seq.d/                       1407   0.0
CHO846 (CHO846Q) /CSM/CH/CHO8-B/CHO846Q.Seq.d/                       1403   0.0
VHN188 (VHN188Q) /CSM/VH/VHN1-D/VHN188Q.Seq.d/                       1398   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ443685) Dictyostelium discoideum cDNA clone:ddv53c23, 3' ...  1415   0.0    1
(BJ384608) Dictyostelium discoideum cDNA clone:ddc50h01, 3' ...  1413   0.0    1
(BJ352822) Dictyostelium discoideum cDNA clone:dda48d04, 3' ...  1413   0.0    1
(BJ384384) Dictyostelium discoideum cDNA clone:ddc49c07, 3' ...  1411   0.0    1
(BJ353047) Dictyostelium discoideum cDNA clone:dda48e21, 3' ...  1411   0.0    1
(BJ401541) Dictyostelium discoideum cDNA clone:dds23j16, 3' ...  1409   0.0    1
(BJ444049) Dictyostelium discoideum cDNA clone:ddv55f01, 3' ...  1407   0.0    1
(BJ384983) Dictyostelium discoideum cDNA clone:ddc51l12, 3' ...  1403   0.0    1
(BJ444943) Dictyostelium discoideum cDNA clone:ddv57o21, 3' ...  1396   0.0    1
(BJ443534) Dictyostelium discoideum cDNA clone:ddv53a12, 3' ...  1396   0.0    1
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   360   8e-98
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   358   3e-97
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   338   3e-91
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   338   3e-91
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           320   9e-86
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   315   2e-84
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   315   2e-84
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   315   2e-84
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   313   7e-84
EF442767_1(EF442767|pid:none) Trypanosoma rangeli isolate H14 cl...   313   1e-83
protein update 2009. 7.15
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHL190 is cyt
5' end seq. ID VHL190F
5' end seq. 
>VHL190F.Seq
ATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAG
CTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAA
ATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTANNNNNNN
NNN
Length of 5' end seq. 603
3' end seq. ID VHL190Z
3' end seq. 
>VHL190Z.Seq
NNNNNNNNNNTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGC
TATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACC
AAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGT
ATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGG
TCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACA
AGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCAC
TGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATC
AACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGA
AAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAG
TAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAA
TGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGA
GGAAAACATCTCTATCAAATAGATTTCTTAAAAAATATAGTAGTAAATCAATAAAGCTTC
TCAT
Length of 3' end seq. 724
Connected seq. ID VHL190P
Connected seq. 
>VHL190P.Seq
ATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTA
TTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTA
TTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAAC
GTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTG
ATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAAC
ATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCA
AAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGA
AAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAG
CTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAA
ATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTA-------
---TCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACC
AAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGT
GTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCT
GAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTT
TCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAA
CAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAA
AATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATT
GAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGAT
GAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTC
TATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCA
CCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAAC
ATCTCTATCAAATAGATTTCTTAAAAAATATAGTAGTAAATCAATAAAGCTTCTCAT
Length of connected seq. 1307
Full length Seq ID -
Full length Seq. -
Length of full length seq. -