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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL183
Library VH
(Link to library)
Clone ID VHL183
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16251-1
Original site URL
Representative seq. ID VHL183P
(Link to Original site)
Representative DNA sequence 
>VHL183 (VHL183Q) /CSM/VH/VHL1-D/VHL183Q.Seq.d/
AATATTACCCAAGNATGATGAAGAAGAACAACAACAACCACCATCAATTTTAATAGTTCA
AACTGAAAAGAGAGATATTGAATCAATGTCATTGACTTTCTCATCAGATCCAGAATCATT
AAATTCTTCATATAGTAATTGTATATTCATAGTTTCAAAAGAACAAAAAGAAAATCCAAC
TTCATATATTCAAGAATATTTTGATATTACTGAAGTATTCTGTGATAACACTACAATTAT
TGAAGCTGGTGATAGTGAATTATTAACAAAGACAATTGAATCAGGTATTGGTAAAAATGA
TATTATTTTCTTTTTAGTTTCACTTGAAGAATTAACAATTGAAAATTATAAACAAGTTAC
AATGCAATATACATTAGTTAATCAAATACTTTTAAGAAATAATCTTTCAACTAGATTTGC
TTTATTAACTTACGATTCTCAAAATGGTGGTAAAAATTATTTAGGATCATCATTAATTGG
TACTTTTAGATACTTTTTAGAATTTCCATCATTGAATACCTTTTCAATTGATGTCGATAA
AGATTCAATTGATAATCTTACATTATTCTTACGTTTAGTTGATCTATCTACAATTGGTGA
TAGAGAAACTATTGTTAGAAATAATAAAATTTTTGTTCAXXXXXXXXXXCTTTTCATCAA
TTGGTGCTATCATTGGTGGTTCAAANCAATGTGCATATAGTAGTGCTAATTTAGTATTGG
ATTCATTATCAAACTATAGAAAGAGTATTGGTCTTGCTTCAACTTCAATTAATTGGGGTG
GTTTGGATGCTGGTGGTGTTGCCGCCACTGATAAATCAGTCGCAAGTTTCTTAGAGGGTC
AAGGTATTTTATTGGTTTCTTTATCAAAGATTTTAGGTTGTCTTGATTCAGTTTTCCAAC
CATCTAATTCCCATTTATCAAACTTTATGCTTTCAAGTTTCAATATTGATAATCTTTTAT
CTTCTGCACCTCAAATGAAGAGAAAAATGGGTCACCACCTAACAAATTATAAAACTTCAT
CTGCTAGCTCTGATGATTCATTGGGTGATTCTAGTTCTACTCAAGCAAAAGTCATTTCTA
CAATCTCTGAATTATTATCAATTCATCCATCAAAATTAAATTTAGATACAAGATTAAAAG
ACTATAGTATTGATTCTTTATTAACAGTACAATTAAAGAATTGGATAGATAAAGAATTTA
CAAAGAATTTATTCACTCATTTACAATTATCATCAAGTAGTATTAATAGTATCATTCAGA
GAATTAGTTCAAAATCAACTTCAACATCTACTCCCAATCCAACTAACACCACAAAACAAA
CCGCAACAACAAAAACATAAGTAGTTCAAACAACTCCAACTACATTNACACTTCTA
sequence update 2002.10.25
Translated Amino Acid sequence 
ILPKXDEEEQQQPPSILIVQTEKRDIESMSLTFSSDPESLNSSYSNCIFIVSKEQKENPT
SYIQEYFDITEVFCDNTTIIEAGDSELLTKTIESGIGKNDIIFFLVSLEELTIENYKQVT
MQYTLVNQILLRNNLSTRFALLTYDSQNGGKNYLGSSLIGTFRYFLEFPSLNTFSIDVDK
DSIDNLTLFLRLVDLSTIGDRETIVRNNKIFV---

---FSSIGAIIGGSXQCAYSSANLVLDSLSNYRKSIGLASTSINWGGLDAGGVAATDKSV
ASFLEGQGILLVSLSKILGCLDSVFQPSNSHLSNFMLSSFNIDNLLSSAPQMKRKMGHHL
TNYKTSSASSDDSLGDSSSTQAKVISTISELLSIHPSKLNLDTRLKDYSIDSLLTVQLKN
WIDKEFTKNLFTHLQLSSSSINSIIQRISSKSTSTSTPNPTNTTKQTATTKT*vvqttpt
txtll
Translated Amino Acid sequence (All Frames) 
Frame A:
nitqx**rrtttttinfnssn*kery*invidflirsriikffi**lyihsfkrtkrksn
fiysrif*yy*sil**hyny*sw***iinkdn*iryw*k*yyflfsft*rinn*kl*tsy
naiyis*sntfkk*sfn*icfinlrfskww*klfriiinwyf*ilfrisiieylfn*cr*
rfn**syiiltfs*siynw**rnyc*k**nfcs---

---lfinwcyhwwfkxmci**c*fsigfiikl*keywscfnfn*lgwfgcwwccrh**is
rkflrgsryfigffikdfrls*fsfpti*fpfiklyafkfqy**sfifctsneekngspp
nkl*nfic*l**fig*f*fysskshfynl*iiinssikikfrykikrl*y*ffinstike
ldr*riykefihsftiiik*y**yhsen*fkinfniysqsn*hhktnrnnknisssnnsn
yixts

Frame B:
ILPKXDEEEQQQPPSILIVQTEKRDIESMSLTFSSDPESLNSSYSNCIFIVSKEQKENPT
SYIQEYFDITEVFCDNTTIIEAGDSELLTKTIESGIGKNDIIFFLVSLEELTIENYKQVT
MQYTLVNQILLRNNLSTRFALLTYDSQNGGKNYLGSSLIGTFRYFLEFPSLNTFSIDVDK
DSIDNLTLFLRLVDLSTIGDRETIVRNNKIFV---

---FSSIGAIIGGSXQCAYSSANLVLDSLSNYRKSIGLASTSINWGGLDAGGVAATDKSV
ASFLEGQGILLVSLSKILGCLDSVFQPSNSHLSNFMLSSFNIDNLLSSAPQMKRKMGHHL
TNYKTSSASSDDSLGDSSSTQAKVISTISELLSIHPSKLNLDTRLKDYSIDSLLTVQLKN
WIDKEFTKNLFTHLQLSSSSINSIIQRISSKSTSTSTPNPTNTTKQTATTKT*vvqttpt
txtll

Frame C:
yypxmmkknnnnhhqf**fklkreilnqch*lshqiqnh*ilhivivys*fqknkkkiql
hifknilillkysvitlqllklvivny*qrqlnqvlvkmilfsf*fhlkn*qlkiinklq
cnih*likyf*eiifqldlly*ltilkmvvkii*dhh*lvlldtf*nfhh*ipfqlmsik
iqliilhysyv*liylqlviekllleiikflf---

---fhqlvlslvvqxnvhivvli*ywihyqtiervlvllqlqligvvwmlvvlpplinqs
qvs*rvkvfywflyqrf*vvliqfsnhlipiyqtlcfqvsiliifyllhlk*rekwvtt*
qiiklhllalmihwvilvllkqksflqslnyyqfihqn*i*iqd*ktivlily*qyn*ri
g*iknlqriysliynyhqvvlivsfrelvqnqlqhllpiqltpqnkpqqqkhk*fkqlql
hxhf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL183 (VHL183Q) /CSM/VH/VHL1-D/VHL183Q.Seq.d/                       2650   0.0
VHO116 (VHO116Q) /CSM/VH/VHO1-A/VHO116Q.Seq.d/                       1421   0.0
VHJ243 (VHJ243Q) /CSM/VH/VHJ2-B/VHJ243Q.Seq.d/                       1421   0.0
VHH639 (VHH639Q) /CSM/VH/VHH6-B/VHH639Q.Seq.d/                       1421   0.0
VHB720 (VHB720Q) /CSM/VH/VHB7-A/VHB720Q.Seq.d/                       1421   0.0
VHB382 (VHB382Q) /CSM/VH/VHB3-D/VHB382Q.Seq.d/                       1421   0.0
VHB174 (VHB174Q) /CSM/VH/VHB1-D/VHB174Q.Seq.d/                       1421   0.0
VHA188 (VHA188Q) /CSM/VH/VHA1-D/VHA188Q.Seq.d/                       1421   0.0
VSD688 (VSD688Q) /CSM/VS/VSD6-D/VSD688Q.Seq.d/                       1419   0.0
VHN484 (VHN484Q) /CSM/VH/VHN4-D/VHN484Q.Seq.d/                       1419   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ424851) Dictyostelium discoideum cDNA clone:ddv53e21, 5' ...  1261   0.0    1
(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...  1239   0.0    1
(BJ443692) Dictyostelium discoideum cDNA clone:ddv53e21, 3' ...  1235   0.0    1
(BJ446822) Dictyostelium discoideum cDNA clone:ddv63o20, 3' ...  1227   0.0    1
(BJ445768) Dictyostelium discoideum cDNA clone:ddv60g17, 3' ...  1227   0.0    1
(BJ445347) Dictyostelium discoideum cDNA clone:ddv59o03, 3' ...  1227   0.0    1
(BJ444858) Dictyostelium discoideum cDNA clone:ddv57l17, 3' ...  1227   0.0    1
(BJ443979) Dictyostelium discoideum cDNA clone:ddv54f22, 3' ...  1227   0.0    1
(BJ443825) Dictyostelium discoideum cDNA clone:ddv54b09, 3' ...  1227   0.0    1
(BJ443717) Dictyostelium discoideum cDNA clone:ddv53j22, 3' ...  1227   0.0    1
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W9) RecName: Full=Probable polyketide synthase 16;         ...   392   e-107
(Q869X2) RecName: Full=Probable polyketide synthase 17;         ...   373   e-101
AC117176_58(AC117176|pid:none) Dictyostelium discoideum chromoso...   119   2e-25
AC117075_22(AC117075|pid:none) Dictyostelium discoideum chromoso...   118   6e-25
(Q54KU5) RecName: Full=Probable polyketide synthase 24;         ...   117   8e-25
AC116982_20(AC116982|pid:none) Dictyostelium discoideum chromoso...   117   1e-24
(Q54TW0) RecName: Full=Probable polyketide synthase 18;         ...   115   5e-24
(Q54KU3) RecName: Full=Probable polyketide synthase 25;         ...   114   8e-24
AC116982_23(AC116982|pid:none) Dictyostelium discoideum chromoso...   112   2e-23
(Q54ED6) RecName: Full=Probable polyketide synthase 41;         ...   107   8e-22
protein update 2009. 7.15
PSORT 

 psg: 0.75  gvh: 0.42  alm: 0.31  top: 0.50  tms: 0.07  mit: 0.17  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: nuclear
	 24.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHL183 is nuc
5' end seq. ID VHL183F
5' end seq. 
>VHL183F.Seq
AATATTACCCAAGNATGATGAAGAAGAACAACAACAACCACCATCAATTTTAATAGTTCA
AACTGAAAAGAGAGATATTGAATCAATGTCATTGACTTTCTCATCAGATCCAGAATCATT
AAATTCTTCATATAGTAATTGTATATTCATAGTTTCAAAAGAACAAAAAGAAAATCCAAC
TTCATATATTCAAGAATATTTTGATATTACTGAAGTATTCTGTGATAACACTACAATTAT
TGAAGCTGGTGATAGTGAATTATTAACAAAGACAATTGAATCAGGTATTGGTAAAAATGA
TATTATTTTCTTTTTAGTTTCACTTGAAGAATTAACAATTGAAAATTATAAACAAGTTAC
AATGCAATATACATTAGTTAATCAAATACTTTTAAGAAATAATCTTTCAACTAGATTTGC
TTTATTAACTTACGATTCTCAAAATGGTGGTAAAAATTATTTAGGATCATCATTAATTGG
TACTTTTAGATACTTTTTAGAATTTCCATCATTGAATACCTTTTCAATTGATGTCGATAA
AGATTCAATTGATAATCTTACATTATTCTTACGTTTAGTTGATCTATCTACAATTGGTGA
TAGAGAAACTATTGTTAGAAATAATAAAATTTTTGTTCANNNNNNNNNN
Length of 5' end seq. 649
3' end seq. ID VHL183Z
3' end seq. 
>VHL183Z.Seq
NNNNNNNNNNCTTTTCATCAATTGGTGCTATCATTGGTGGTTCAAANCAATGTGCATATA
GTAGTGCTAATTTAGTATTGGATTCATTATCAAACTATAGAAAGAGTATTGGTCTTGCTT
CAACTTCAATTAATTGGGGTGGTTTGGATGCTGGTGGTGTTGCCGCCACTGATAAATCAG
TCGCAAGTTTCTTAGAGGGTCAAGGTATTTTATTGGTTTCTTTATCAAAGATTTTAGGTT
GTCTTGATTCAGTTTTCCAACCATCTAATTCCCATTTATCAAACTTTATGCTTTCAAGTT
TCAATATTGATAATCTTTTATCTTCTGCACCTCAAATGAAGAGAAAAATGGGTCACCACC
TAACAAATTATAAAACTTCATCTGCTAGCTCTGATGATTCATTGGGTGATTCTAGTTCTA
CTCAAGCAAAAGTCATTTCTACAATCTCTGAATTATTATCAATTCATCCATCAAAATTAA
ATTTAGATACAAGATTAAAAGACTATAGTATTGATTCTTTATTAACAGTACAATTAAAGA
ATTGGATAGATAAAGAATTTACAAAGAATTTATTCACTCATTTACAATTATCATCAAGTA
GTATTAATAGTATCATTCAGAGAATTAGTTCAAAATCAACTTCAACATCTACTCCCAATC
CAACTAACACCACAAAACAAACCGCAACAACAAAAACATAAGTAGTTCAAACAACTCCAA
CTACATTNACACTTCTA
Length of 3' end seq. 737
Connected seq. ID VHL183P
Connected seq. 
>VHL183P.Seq
AATATTACCCAAGNATGATGAAGAAGAACAACAACAACCACCATCAATTTTAATAGTTCA
AACTGAAAAGAGAGATATTGAATCAATGTCATTGACTTTCTCATCAGATCCAGAATCATT
AAATTCTTCATATAGTAATTGTATATTCATAGTTTCAAAAGAACAAAAAGAAAATCCAAC
TTCATATATTCAAGAATATTTTGATATTACTGAAGTATTCTGTGATAACACTACAATTAT
TGAAGCTGGTGATAGTGAATTATTAACAAAGACAATTGAATCAGGTATTGGTAAAAATGA
TATTATTTTCTTTTTAGTTTCACTTGAAGAATTAACAATTGAAAATTATAAACAAGTTAC
AATGCAATATACATTAGTTAATCAAATACTTTTAAGAAATAATCTTTCAACTAGATTTGC
TTTATTAACTTACGATTCTCAAAATGGTGGTAAAAATTATTTAGGATCATCATTAATTGG
TACTTTTAGATACTTTTTAGAATTTCCATCATTGAATACCTTTTCAATTGATGTCGATAA
AGATTCAATTGATAATCTTACATTATTCTTACGTTTAGTTGATCTATCTACAATTGGTGA
TAGAGAAACTATTGTTAGAAATAATAAAATTTTTGTTCA----------CTTTTCATCAA
TTGGTGCTATCATTGGTGGTTCAAANCAATGTGCATATAGTAGTGCTAATTTAGTATTGG
ATTCATTATCAAACTATAGAAAGAGTATTGGTCTTGCTTCAACTTCAATTAATTGGGGTG
GTTTGGATGCTGGTGGTGTTGCCGCCACTGATAAATCAGTCGCAAGTTTCTTAGAGGGTC
AAGGTATTTTATTGGTTTCTTTATCAAAGATTTTAGGTTGTCTTGATTCAGTTTTCCAAC
CATCTAATTCCCATTTATCAAACTTTATGCTTTCAAGTTTCAATATTGATAATCTTTTAT
CTTCTGCACCTCAAATGAAGAGAAAAATGGGTCACCACCTAACAAATTATAAAACTTCAT
CTGCTAGCTCTGATGATTCATTGGGTGATTCTAGTTCTACTCAAGCAAAAGTCATTTCTA
CAATCTCTGAATTATTATCAATTCATCCATCAAAATTAAATTTAGATACAAGATTAAAAG
ACTATAGTATTGATTCTTTATTAACAGTACAATTAAAGAATTGGATAGATAAAGAATTTA
CAAAGAATTTATTCACTCATTTACAATTATCATCAAGTAGTATTAATAGTATCATTCAGA
GAATTAGTTCAAAATCAACTTCAACATCTACTCCCAATCCAACTAACACCACAAAACAAA
CCGCAACAACAAAAACATAAGTAGTTCAAACAACTCCAACTACATTNACACTTCTA
Length of connected seq. 1366
Full length Seq ID -
Full length Seq. -
Length of full length seq. -