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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL171
Library VH
(Link to library)
Clone ID VHL171
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12522-1
Original site URL
Representative seq. ID VHL171P
(Link to Original site)
Representative DNA sequence 
>VHL171 (VHL171Q) /CSM/VH/VHL1-C/VHL171Q.Seq.d/
CACTGTTGGCCTACTGGTAATATCAATTGATTGGAAAAAAGAAGAAGAAAAAAAAATGTT
AGTTTTAATTAAAACACCAACTATAACATTTTCATTGGATATTGAAATTTCAGATACCTT
GTCAGTATTATATAACAAGGTTGCAGAGAGAACAAAAAATAAAAGCTATCAATTTCAATT
ACACTTTGCTGGTGATGTTTTATCAAATATCTTATCAAAAGCAATTGGAGCATGTGGTAT
TAAAAAAGAGTCAACAATCCATATGATCTTAATTAAAGAGATTAAATTAACTATTAAATA
TTTAGATAAAGATTATCCAGTTGAAATTCCAAATAATGCAGGTTTAAAAGTTTCACATTT
AGTATCTCATATTAGAACATCATTACAAACTCAAATACCATTATTACGTTCATATTTAAA
CTCTGTTAGTTTATATAAAACTGGTACAGATACTAAATATACAGAGAGTATGAGTTTACA
AGGAGAATATTAATTGTTATGATACTATTGATATGAAAATTGGAACACCACTACCTACAA
CAACGACAACAACAACAACAACAGGATCAACATTAAATXXXXXXXXXXAAAGAAGAGCAG
AAATTGTAGAATCAAATATAACTGATGGTAAATCAACATCATCATCATCACCATCATCAT
CATCATCATCATTAACAATAACTTCATCAATGAATTCAATGAAATTATTTGATTTTGAAA
GTAGAATCATTAATGAAACAAATTTAGAAACAATAAAAAAGAATAAAGTGACAAATAAAG
AGAAAATAATTTATATAATTGGTGATTCAGGAATTGGTAAAACTACATTTGTAAATAATT
TAAACTATGTAGGTAAATCATATAATAGTAAACTTAGATTTAAAGAGAAATCAACATCAT
TATCACAATCGGATTTTGATACTACAGATTTGTTTATTGTGTGTTTCACTCTACAAGGTT
CAAGTAATTCATATAATAACTTTACAAATTATTTCACAGAGATTCGTAAACGTGATCCAA
TGAAACCAATTGCAATAGCACTACTTCAAAATGATTTAATACCCGATTTAAATAATAAAG
CAACTTATCAGCCCTCTGCACTTCAACAATTATATTCATTGGCTGGAACATTAGAATTCT
CTTCAAAAATGCCACAAAATCAAATTAAATTAATTGAAAAATCAATAAATTCGAAATTAT
CATCAACTTCATCATCATCATCATCATCTTGTATTTAATGTAAAGTTATAAAACTAAAAA
ATAAACTAAAANAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
llayw*yqligkkkkkkkc*f*lkhql*hfhwilkfqipcqyyitrlqreqkikainfny
tllvmfyqisyqkqlehvvlkksqqsi*s*lkrln*llni*ikiiqlkfqimqv*kfhi*
ylilehhyklkyhyyvhi*tllvyiklvqilniqrv*VYKENINCYDTIDMKIGTPLPTT
TTTTTTTGSTLN---

---RRAEIVESNITDGKSTSSSSPSSSSSSLTITSSMNSMKLFDFESRIINETNLETIKK
NKVTNKEKIIYIIGDSGIGKTTFVNNLNYVGKSYNSKLRFKEKSTSLSQSDFDTTDLFIV
CFTLQGSSNSYNNFTNYFTEIRKRDPMKPIAIALLQNDLIPDLNNKATYQPSALQQLYSL
AGTLEFSSKMPQNQIKLIEKSINSKLSSTSSSSSSSCI*ckviklknklkxk
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgnin*lekrrrkknvsfn*ntnynifigy*nfrylvsii*qgcrenkk*klsisi
tlcw*cfikyliksnwsmwy*krvnnpydln*rd*iny*ifr*rlss*nsk*crfksftf
sisy*niitnsntiitfifklc*fi*nwyry*iyreyeftrrilivmilli*klehhylq
qrqqqqqqdqh*---

---keeqkl*nqi*lmvnqhhhhhhhhhhhh*q*lhq*iq*nylilkveslmkqi*kq*k
rik*qikrk*fi*lviqelvklhl*ii*tm*vnhiivnldlkrnqhhyhnrililqicll
cvslykvqvihiitlqiisqrfvnviq*nqlq*hyfkmi*ypi*iikqlisplhfnnyih
wleh*nslqkchkikln*lknq*irnyhqlhhhhhhlvfnvkl*n*kin*xkk

Frame B:
tvgllvisidwkkeeekkmlvliktptitfsldieisdtlsvlynkvaertknksyqfql
hfagdvlsnilskaigacgikkestihmilikeikltikyldkdypveipnnaglkvshl
vshirtslqtqipllrsylnsvslyktgtdtkytesmslqgey*ll*yy*yenwntttyn
ndnnnnnrinik---

---kksrncrikyn*w*iniiiitiiiiiiinnnfinefneii*f*k*nh**nkfrnnkk
e*sdk*rennlynw*frnw*nyick*fklcr*ii***t*i*reiniiitigf*yyrfvyc
vfhstrfk*fi**lyklfhrds*t*snetncnsttsk*fntrfk**snlsalctstiifi
gwnirilfknatksn*in*kinkfeiiinfiiiiiilylm*syktkk*tkxkk

Frame C:
llayw*yqligkkkkkkkc*f*lkhql*hfhwilkfqipcqyyitrlqreqkikainfny
tllvmfyqisyqkqlehvvlkksqqsi*s*lkrln*llni*ikiiqlkfqimqv*kfhi*
ylilehhyklkyhyyvhi*tllvyiklvqilniqrv*VYKENINCYDTIDMKIGTPLPTT
TTTTTTTGSTLN---

---RRAEIVESNITDGKSTSSSSPSSSSSSLTITSSMNSMKLFDFESRIINETNLETIKK
NKVTNKEKIIYIIGDSGIGKTTFVNNLNYVGKSYNSKLRFKEKSTSLSQSDFDTTDLFIV
CFTLQGSSNSYNNFTNYFTEIRKRDPMKPIAIALLQNDLIPDLNNKATYQPSALQQLYSL
AGTLEFSSKMPQNQIKLIEKSINSKLSSTSSSSSSSCI*ckviklknklkxk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL171 (VHL171Q) /CSM/VH/VHL1-C/VHL171Q.Seq.d/                       1009   0.0
VSA771 (VSA771Q) /CSM/VS/VSA7-C/VSA771Q.Seq.d/                        321   1e-86
AHM885 (AHM885Q) /CSM/AH/AHM8-D/AHM885Q.Seq.d/                         48   3e-04
VHL332 (VHL332Q) /CSM/VH/VHL3-B/VHL332Q.Seq.d/                         46   0.001
VHH468 (VHH468Q) /CSM/VH/VHH4-C/VHH468Q.Seq.d/                         46   0.001
VHA356 (VHA356Q) /CSM/VH/VHA3-C/VHA356Q.Seq.d/                         46   0.001
AHK166 (AHK166Q) /CSM/AH/AHK1-C/AHK166Q.Seq.d/                         46   0.001
VFM830 (VFM830Q) /CSM/VF/VFM8-B/VFM830Q.Seq.d/                         44   0.005
VFD495 (VFD495Q) /CSM/VF/VFD4-D/VFD495Q.Seq.d/                         44   0.005
VHM692 (VHM692Q) /CSM/VH/VHM6-D/VHM692Q.Seq.d/                         42   0.021
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116977) Dictyostelium discoideum chromosome 2 map 5515173...   682   0.0    9
(BJ424814) Dictyostelium discoideum cDNA clone:ddv53m17, 5' ...   952   0.0    3
(BJ443659) Dictyostelium discoideum cDNA clone:ddv53m17, 3' ...   682   0.0    4
(AU261726) Dictyostelium discoideum vegetative cDNA clone:VS...   230   4e-85  3
(BX293980) Mycoplasma mycoides subsp. mycoides SC str. PG1, ...    34   0.12   19
(U66911) Dictyostelium discoideum ORF DG1007 gene, partial cds.    36   0.14   3
(CI031426) Oryza sativa Japonica Group cDNA, clone: 009-M087...    46   0.18   2
(BJ418120) Dictyostelium discoideum cDNA clone:ddv31p13, 5' ...    46   0.19   2
(FH373229) CHO_OF4530xd05r1.ab1 CHO_OF4 Nicotiana tabacum ge...    50   0.21   1
(FH322268) CHO_OF4542xp09r1.ab1 CHO_OF4 Nicotiana tabacum ge...    50   0.21   1
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116977_41(AC116977|pid:none) Dictyostelium discoideum chromoso...   304   4e-81
AC116305_104(AC116305|pid:none) Dictyostelium discoideum chromos...    95   6e-18
AF250284_180(AF250284|pid:none) Amsacta moorei entomopoxvirus, c...    45   0.007
AF162221_52(AF162221|pid:none) Xestia c-nigrum granulovirus geno...    43   0.021
EU255577_47(EU255577|pid:none) Helicoverpa armigera granulovirus...    43   0.021
AJ245512_1(AJ245512|pid:none) Glomus mosseae partial mRNA for ub...    42   0.048
AY099140_1(AY099140|pid:none) Cercomonas ATCC50316 isolate pub2 ...    42   0.062
AF063866_144(AF063866|pid:none) Melanoplus sanguinipes entomopox...    42   0.062
FJ227128_48(FJ227128|pid:none) Euproctis pseudoconspersa nucleop...    42   0.062
EF035042_123(EF035042|pid:none) Spodoptera frugiperda MNPV isola...    42   0.062
protein update 2009. 7.15
PSORT 

 psg: 0.75  gvh: 0.40  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VHL171 is cyt
5' end seq. ID VHL171F
5' end seq. 
>VHL171F.Seq
CACTGTTGGCCTACTGGTAATATCAATTGATTGGAAAAAAGAAGAAGAAAAAAAAATGTT
AGTTTTAATTAAAACACCAACTATAACATTTTCATTGGATATTGAAATTTCAGATACCTT
GTCAGTATTATATAACAAGGTTGCAGAGAGAACAAAAAATAAAAGCTATCAATTTCAATT
ACACTTTGCTGGTGATGTTTTATCAAATATCTTATCAAAAGCAATTGGAGCATGTGGTAT
TAAAAAAGAGTCAACAATCCATATGATCTTAATTAAAGAGATTAAATTAACTATTAAATA
TTTAGATAAAGATTATCCAGTTGAAATTCCAAATAATGCAGGTTTAAAAGTTTCACATTT
AGTATCTCATATTAGAACATCATTACAAACTCAAATACCATTATTACGTTCATATTTAAA
CTCTGTTAGTTTATATAAAACTGGTACAGATACTAAATATACAGAGAGTATGAGTTTACA
AGGAGAATATTAATTGTTATGATACTATTGATATGAAAATTGGAACACCACTACCTACAA
CAACGACAACAACAACAACAACAGGATCAACATTAAATNNNNNNNNNN
Length of 5' end seq. 588
3' end seq. ID VHL171Z
3' end seq. 
>VHL171Z.Seq
NNNNNNNNNNAAAGAAGAGCAGAAATTGTAGAATCAAATATAACTGATGGTAAATCAACA
TCATCATCATCACCATCATCATCATCATCATCATTAACAATAACTTCATCAATGAATTCA
ATGAAATTATTTGATTTTGAAAGTAGAATCATTAATGAAACAAATTTAGAAACAATAAAA
AAGAATAAAGTGACAAATAAAGAGAAAATAATTTATATAATTGGTGATTCAGGAATTGGT
AAAACTACATTTGTAAATAATTTAAACTATGTAGGTAAATCATATAATAGTAAACTTAGA
TTTAAAGAGAAATCAACATCATTATCACAATCGGATTTTGATACTACAGATTTGTTTATT
GTGTGTTTCACTCTACAAGGTTCAAGTAATTCATATAATAACTTTACAAATTATTTCACA
GAGATTCGTAAACGTGATCCAATGAAACCAATTGCAATAGCACTACTTCAAAATGATTTA
ATACCCGATTTAAATAATAAAGCAACTTATCAGCCCTCTGCACTTCAACAATTATATTCA
TTGGCTGGAACATTAGAATTCTCTTCAAAAATGCCACAAAATCAAATTAAATTAATTGAA
AAATCAATAAATTCGAAATTATCATCAACTTCATCATCATCATCATCATCTTGTATTTAA
TGTAAAGTTATAAAACTAAAAAATAAACTAAAANAAAAAAA
Length of 3' end seq. 701
Connected seq. ID VHL171P
Connected seq. 
>VHL171P.Seq
CACTGTTGGCCTACTGGTAATATCAATTGATTGGAAAAAAGAAGAAGAAAAAAAAATGTT
AGTTTTAATTAAAACACCAACTATAACATTTTCATTGGATATTGAAATTTCAGATACCTT
GTCAGTATTATATAACAAGGTTGCAGAGAGAACAAAAAATAAAAGCTATCAATTTCAATT
ACACTTTGCTGGTGATGTTTTATCAAATATCTTATCAAAAGCAATTGGAGCATGTGGTAT
TAAAAAAGAGTCAACAATCCATATGATCTTAATTAAAGAGATTAAATTAACTATTAAATA
TTTAGATAAAGATTATCCAGTTGAAATTCCAAATAATGCAGGTTTAAAAGTTTCACATTT
AGTATCTCATATTAGAACATCATTACAAACTCAAATACCATTATTACGTTCATATTTAAA
CTCTGTTAGTTTATATAAAACTGGTACAGATACTAAATATACAGAGAGTATGAGTTTACA
AGGAGAATATTAATTGTTATGATACTATTGATATGAAAATTGGAACACCACTACCTACAA
CAACGACAACAACAACAACAACAGGATCAACATTAAAT----------AAAGAAGAGCAG
AAATTGTAGAATCAAATATAACTGATGGTAAATCAACATCATCATCATCACCATCATCAT
CATCATCATCATTAACAATAACTTCATCAATGAATTCAATGAAATTATTTGATTTTGAAA
GTAGAATCATTAATGAAACAAATTTAGAAACAATAAAAAAGAATAAAGTGACAAATAAAG
AGAAAATAATTTATATAATTGGTGATTCAGGAATTGGTAAAACTACATTTGTAAATAATT
TAAACTATGTAGGTAAATCATATAATAGTAAACTTAGATTTAAAGAGAAATCAACATCAT
TATCACAATCGGATTTTGATACTACAGATTTGTTTATTGTGTGTTTCACTCTACAAGGTT
CAAGTAATTCATATAATAACTTTACAAATTATTTCACAGAGATTCGTAAACGTGATCCAA
TGAAACCAATTGCAATAGCACTACTTCAAAATGATTTAATACCCGATTTAAATAATAAAG
CAACTTATCAGCCCTCTGCACTTCAACAATTATATTCATTGGCTGGAACATTAGAATTCT
CTTCAAAAATGCCACAAAATCAAATTAAATTAATTGAAAAATCAATAAATTCGAAATTAT
CATCAACTTCATCATCATCATCATCATCTTGTATTTAATGTAAAGTTATAAAACTAAAAA
ATAAACTAAAANAAAAAAA
Length of connected seq. 1269
Full length Seq ID -
Full length Seq. -
Length of full length seq. -