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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL154
Library VH
(Link to library)
Clone ID VHL154
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16579-1
Original site URL
Representative seq. ID VHL154P
(Link to Original site)
Representative DNA sequence 
>VHL154 (VHL154Q) /CSM/VH/VHL1-C/VHL154Q.Seq.d/
ATGTGCAATGAAAAATAGGAGCTCTTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATG
TGGGTTGAACCAAATATTGGGATAAGACGTCTAACATTCACTAATAGATACCACAAAAGG
TGTTAGTTCATTAAGACAGCAGGACGGTGGCCATGGAAGTCGGTATCCGCTAAGGAGTGT
GTAACAACTCACCTGCCAAATGGACTAGCCCTGAAAATGGATGACGCTAGCAGTGGATGG
TCGATGCCCAATCGTTAAAAGAAGTGATAATACTTTTAACGTGTAGGAAGGCGTGAAGGT
AACGTAGAAGCTTGAATGTGAATTCGAGTGGAGTTGTCTTTAGTGCAGATCTTGATGGTA
GTAGCAAATATTCAAAAGAATTTACTTTGAAGGCCGAAGTGGGGAAGGGTTCCATAACAA
TGGAATTCACTTATGGGTGAGTCGATCCTAAGGTTTGGGTTAACTCTCTCTAATAAGGTT
ACTAGGTCATTGGATCGAAAGTGAAGGTGGCTTTAACACTAGTGACTTTATAGGCCGAAA
GGGAAGCGGGTTAAAATTCCTGCACCATCGAATGGGATATTAGGGTAACCGATCGTAAXX
XXXXXXXXAGAGTAAAAGGGCAAAAGCCTGCTTGATTCTGATTTTCAGTACTAATCGGAA
CTGGGAAACCAGGGCCTATCGATCCTTTATGTGCTTAAATCTTAAGCCTAGAGGTGTCAG
AAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGCTCATAGCGACGCTGCTTT
TTGATCCTTCGATGTCGGCTCTTCTTATCATTGTGAAGCAGAATTCACAAAGTGTTGGAT
TGTTCACCCACTAACAAGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT
TACCCTACTGTTGTCAATTGTTTGCGTAATAGTAGCATGATTTAGTACGAGAGGAACTGT
CATGCCGGATCACTGGTCTGTAGGTTTATTTGACAAAATAGTGACCTGCCGCTACCATCC
GTTGGATAATGGCTGAACGCCTCTAAGTCAGAATCCATTCTAGAAACGCAAACCAAATGC
TTTAGAGTGTGAATGTTGTAGGTAACATTAGGTTGTTGGTGGGGGACCACTTTCAACTTT
AAACCATATGATTAATCGCTGTTACACTGCAGTTTCCTTCCGGTTATTGTGGTGGGTGGC
TAAATTCTAATTTATATCCTCGTTCCGCTCAACTCTTCGATTGTAGACGACTATCAAATG
AACTAGGTGCTGTAAGCTTCCGAGTAGCGTTCAGTTACGAGGGGTNAGGCTTTTCCAT
sequence update 2002.10.25
Translated Amino Acid sequence 
vq*kigalsgpflvsrtgdvg*tkywdktsnih**ipqkvlvh*dsrtvamevgir*gvc
nnspakwtspeng*r*qwmvdaqslkeviilltcrka*r*rrslnvnssgvvfsadldgs
skyskeftlkaevgkgsitmeftyg*vdpkvwvnsl**gy*vigskvkvaltlvtl*aer
eag*NSCTIEWDIRVTDR---

---SKRAKACLILIFSTNRNWETRAYRSFMCLNLKPRGVRKVTTGITGLWQPSAHSDAAF
*sfdvgssyhceaeftkcwivhpltrnvswv*tvvrqvsftlllsivcviva*fstrgtv
mpdhwsvglfdkivtcryhpldng*tplsqnpf*krkpnalececcr*h*vvgggplstl
nhminrcytavsfrllwwvakf*fissfrstlrl*ttik*trccklpssvqlrgvrlfh
Translated Amino Acid sequence (All Frames) 
Frame A:
mcnek*ellvghfw*aelamwvepnigirrltftnryhkrc*fiktagrwpwksvsakec
vtthlpnglalkmddassgwsmpnr*kk**yf*rvgrregnvea*m*irvelslvqilmv
vaniqknll*rpkwgrvp*qwnslmgesilrfgltlsnkvtrsldrk*rwl*h**lyrpk
gkrvkipapsngilg*piv---

---rvkgqkpa*f*fsvligtgkpgpidplca*ilslevseklpqg*lacgsqaliatll
fdpsmsalliivkqnsqsvglfth*qgt*agfrps*drlvlpyccqlfa***hdlvreel
scritgl*vyltk**paatirwimaerl*vrihsrnanqml*svnvvgnirllvgdhfql
*ti*liavtlqfpsgycggwlnsnlyprsaqlfdcrrlsnelgavsfrvafsyegxgfs

Frame B:
camknrss*waifgkqnwrcglnqilg*dv*hslidttkgvsslrqqdgghgsryplrsv
*qltcqmd*p*kwmtlavdgrcpivkrsdntfnv*egvkvt*klecefewscl*crs*w*
*qifkriyfegrsgegfhnngihlwvsrs*glg*lslirllghwieseggfntsdfigrk
gsglkflhhrmgy*gnrs*---

---e*kgkslldsdfqy*selgnqglsilyvlks*a*rcqksyhrdnwlvaakrs*rrcf
lilrcrlflsl*srihkvldcsptnkerelgldrretg*fyptvvnclrnssmi*yernc
hagslvcrfi*qnsdlplpsvg*wlnasksesiletqtkcfrv*ml*vtlgcwwgttfnf
kpyd*sllhcsflpvivvgg*iliyilvplnssivddyqmn*vl*ase*rsvtrgxafp

Frame C:
vq*kigalsgpflvsrtgdvg*tkywdktsnih**ipqkvlvh*dsrtvamevgir*gvc
nnspakwtspeng*r*qwmvdaqslkeviilltcrka*r*rrslnvnssgvvfsadldgs
skyskeftlkaevgkgsitmeftyg*vdpkvwvnsl**gy*vigskvkvaltlvtl*aer
eag*NSCTIEWDIRVTDR---

---SKRAKACLILIFSTNRNWETRAYRSFMCLNLKPRGVRKVTTGITGLWQPSAHSDAAF
*sfdvgssyhceaeftkcwivhpltrnvswv*tvvrqvsftlllsivcviva*fstrgtv
mpdhwsvglfdkivtcryhpldng*tplsqnpf*krkpnalececcr*h*vvgggplstl
nhminrcytavsfrllwwvakf*fissfrstlrl*ttik*trccklpssvqlrgvrlfh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL154 (VHL154Q) /CSM/VH/VHL1-C/VHL154Q.Seq.d/                       2547   0.0
VHM596 (VHM596Q) /CSM/VH/VHM5-D/VHM596Q.Seq.d/                       1396   0.0
VHM406 (VHM406Q) /CSM/VH/VHM4-A/VHM406Q.Seq.d/                       1396   0.0
VHK648 (VHK648Q) /CSM/VH/VHK6-B/VHK648Q.Seq.d/                       1392   0.0
VHQ477 (VHQ477Q) /CSM/VH/VHQ4-D/VHQ477Q.Seq.d/                       1388   0.0
VHI207 (VHI207Q) /CSM/VH/VHI2-A/VHI207Q.Seq.d/                       1388   0.0
VHD592 (VHD592Q) /CSM/VH/VHD5-D/VHD592Q.Seq.d/                       1388   0.0
VHA465 (VHA465Q) /CSM/VH/VHA4-C/VHA465Q.Seq.d/                       1388   0.0
SLE369 (SLE369Q) /CSM/SL/SLE3-C/SLE369Q.Seq.d/                       1388   0.0
SHA880 (SHA880Q) /CSM/SH/SHA8-D/SHA880Q.Seq.d/                       1388   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ443646) Dictyostelium discoideum cDNA clone:ddv53k13, 3' ...  1402   0.0    1
(BJ444622) Dictyostelium discoideum cDNA clone:ddv56o23, 3' ...  1396   0.0    1
(BJ444075) Dictyostelium discoideum cDNA clone:ddv55l02, 3' ...  1396   0.0    1
(BJ443278) Dictyostelium discoideum cDNA clone:ddv52o12, 3' ...  1392   0.0    1
(AY171066) Dictyostelium discoideum extrachromosomal palindr...  1382   0.0    1
(BJ446798) Dictyostelium discoideum cDNA clone:ddv63j20, 3' ...  1382   0.0    1
(BJ443266) Dictyostelium discoideum cDNA clone:ddv52l12, 3' ...  1382   0.0    1
(BJ443056) Dictyostelium discoideum cDNA clone:ddv51c23, 3' ...  1382   0.0    1
(BJ441877) Dictyostelium discoideum cDNA clone:ddv48k05, 3' ...  1382   0.0    1
(BJ441582) Dictyostelium discoideum cDNA clone:ddv47m02, 3' ...  1382   0.0    1
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU430725_1(EU430725|pid:none) Cupressus sempervirens clone CYPLP...   140   1e-31
AY436773_1(AY436773|pid:none) Pyrus communis putative senescence...   103   1e-20
CP001575_406(CP001575|pid:none) Micromonas sp. RCC299 chromosome...   101   5e-20
AK230153_1(AK230153|pid:none) Arabidopsis thaliana mRNA for hypo...    99   4e-19
CP000497_644(CP000497|pid:none) Pichia stipitis CBS 6054 chromos...    60   1e-13
EF192058_1(EF192058|pid:none) Trichosanthes dioica clone TDM04 p...    77   1e-12
EU244380_1(EU244380|pid:none) Haliotis diversicolor clone HDr4CJ...    73   2e-11
BC114377_1(BC114377|pid:none) Homo sapiens cDNA clone MGC:134704...    68   8e-10
AL844507_287(AL844507|pid:none) Plasmodium falciparum 3D7 chromo...    64   1e-08
U12823_1(U12823|pid:none) Acanthamoeba polyphaga CDC:0187:1 hemo...    45   4e-05
protein update 2009. 7.15
PSORT 

 psg: 0.75  gvh: 0.37  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 40.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for VHL154 is cyt
5' end seq. ID VHL154F
5' end seq. 
>VHL154F.Seq
ATGTGCAATGAAAAATAGGAGCTCTTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATG
TGGGTTGAACCAAATATTGGGATAAGACGTCTAACATTCACTAATAGATACCACAAAAGG
TGTTAGTTCATTAAGACAGCAGGACGGTGGCCATGGAAGTCGGTATCCGCTAAGGAGTGT
GTAACAACTCACCTGCCAAATGGACTAGCCCTGAAAATGGATGACGCTAGCAGTGGATGG
TCGATGCCCAATCGTTAAAAGAAGTGATAATACTTTTAACGTGTAGGAAGGCGTGAAGGT
AACGTAGAAGCTTGAATGTGAATTCGAGTGGAGTTGTCTTTAGTGCAGATCTTGATGGTA
GTAGCAAATATTCAAAAGAATTTACTTTGAAGGCCGAAGTGGGGAAGGGTTCCATAACAA
TGGAATTCACTTATGGGTGAGTCGATCCTAAGGTTTGGGTTAACTCTCTCTAATAAGGTT
ACTAGGTCATTGGATCGAAAGTGAAGGTGGCTTTAACACTAGTGACTTTATAGGCCGAAA
GGGAAGCGGGTTAAAATTCCTGCACCATCGAATGGGATATTAGGGTAACCGATCGTAANN
NNNNNNNN
Length of 5' end seq. 608
3' end seq. ID VHL154Z
3' end seq. 
>VHL154Z.Seq
NNNNNNNNNNAGAGTAAAAGGGCAAAAGCCTGCTTGATTCTGATTTTCAGTACTAATCGG
AACTGGGAAACCAGGGCCTATCGATCCTTTATGTGCTTAAATCTTAAGCCTAGAGGTGTC
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGCTCATAGCGACGCTGCT
TTTTGATCCTTCGATGTCGGCTCTTCTTATCATTGTGAAGCAGAATTCACAAAGTGTTGG
ATTGTTCACCCACTAACAAGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGT
TTTACCCTACTGTTGTCAATTGTTTGCGTAATAGTAGCATGATTTAGTACGAGAGGAACT
GTCATGCCGGATCACTGGTCTGTAGGTTTATTTGACAAAATAGTGACCTGCCGCTACCAT
CCGTTGGATAATGGCTGAACGCCTCTAAGTCAGAATCCATTCTAGAAACGCAAACCAAAT
GCTTTAGAGTGTGAATGTTGTAGGTAACATTAGGTTGTTGGTGGGGGACCACTTTCAACT
TTAAACCATATGATTAATCGCTGTTACACTGCAGTTTCCTTCCGGTTATTGTGGTGGGTG
GCTAAATTCTAATTTATATCCTCGTTCCGCTCAACTCTTCGATTGTAGACGACTATCAAA
TGAACTAGGTGCTGTAAGCTTCCGAGTAGCGTTCAGTTACGAGGGGTNAGGCTTTTCCAT
Length of 3' end seq. 720
Connected seq. ID VHL154P
Connected seq. 
>VHL154P.Seq
ATGTGCAATGAAAAATAGGAGCTCTTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATG
TGGGTTGAACCAAATATTGGGATAAGACGTCTAACATTCACTAATAGATACCACAAAAGG
TGTTAGTTCATTAAGACAGCAGGACGGTGGCCATGGAAGTCGGTATCCGCTAAGGAGTGT
GTAACAACTCACCTGCCAAATGGACTAGCCCTGAAAATGGATGACGCTAGCAGTGGATGG
TCGATGCCCAATCGTTAAAAGAAGTGATAATACTTTTAACGTGTAGGAAGGCGTGAAGGT
AACGTAGAAGCTTGAATGTGAATTCGAGTGGAGTTGTCTTTAGTGCAGATCTTGATGGTA
GTAGCAAATATTCAAAAGAATTTACTTTGAAGGCCGAAGTGGGGAAGGGTTCCATAACAA
TGGAATTCACTTATGGGTGAGTCGATCCTAAGGTTTGGGTTAACTCTCTCTAATAAGGTT
ACTAGGTCATTGGATCGAAAGTGAAGGTGGCTTTAACACTAGTGACTTTATAGGCCGAAA
GGGAAGCGGGTTAAAATTCCTGCACCATCGAATGGGATATTAGGGTAACCGATCGTAA--
--------AGAGTAAAAGGGCAAAAGCCTGCTTGATTCTGATTTTCAGTACTAATCGGAA
CTGGGAAACCAGGGCCTATCGATCCTTTATGTGCTTAAATCTTAAGCCTAGAGGTGTCAG
AAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGCTCATAGCGACGCTGCTTT
TTGATCCTTCGATGTCGGCTCTTCTTATCATTGTGAAGCAGAATTCACAAAGTGTTGGAT
TGTTCACCCACTAACAAGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTT
TACCCTACTGTTGTCAATTGTTTGCGTAATAGTAGCATGATTTAGTACGAGAGGAACTGT
CATGCCGGATCACTGGTCTGTAGGTTTATTTGACAAAATAGTGACCTGCCGCTACCATCC
GTTGGATAATGGCTGAACGCCTCTAAGTCAGAATCCATTCTAGAAACGCAAACCAAATGC
TTTAGAGTGTGAATGTTGTAGGTAACATTAGGTTGTTGGTGGGGGACCACTTTCAACTTT
AAACCATATGATTAATCGCTGTTACACTGCAGTTTCCTTCCGGTTATTGTGGTGGGTGGC
TAAATTCTAATTTATATCCTCGTTCCGCTCAACTCTTCGATTGTAGACGACTATCAAATG
AACTAGGTGCTGTAAGCTTCCGAGTAGCGTTCAGTTACGAGGGGTNAGGCTTTTCCAT
Length of connected seq. 1308
Full length Seq ID -
Full length Seq. -
Length of full length seq. -