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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL133
Library VH
(Link to library)
Clone ID VHL133
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15754-1
Original site URL
Representative seq. ID VHL133P
(Link to Original site)
Representative DNA sequence 
>VHL133 (VHL133Q) /CSM/VH/VHL1-B/VHL133Q.Seq.d/
TCACAATAATAATGGAACAAAAAAGATTAGATGATTCATTATTAAGAAATTCTGATTGGA
AGAATTGGGGTACCTATGTAAGTGATAGACAATGGGCAACTGTTCGTGAAGATTATGGTG
GTGAACCATGGACCTACTTTCCACACGATCATGCTAGATCACGTGCATATAGATGGTCAG
AGGATAGTATTGGTGGATTTTGTAATAGATTTCAAAATATTTGTTTAGGTTTAGCATTTT
GGAATGAAAAAGATCCAATTATTAAAGAGAGATTTTTTGGACTTGGTGGACCAGAAGGAA
ATCATGGTGAAGATGTAAAAGAACATTATTTCTATTTAGATAATACACCAACACATTCCT
ATGCCAAAATGTTATATAAATATCCAATGAATGAATATCCATATAGTGATTTAGTATCAA
CCAATGCACAACGTGGTAAACAAGATCCAGAGTATGAAATTGAAGATACTGGTATCTTTG
ATAAGGATGAATATTTCGATTGTTACATTGAATATGCTAAAGGAGATCAAGAAGATGATA
TTTTATGTAAAGTTACCGTACATAATCGTTCAXXXXXXXXXXTGTTTGCTGCTGAAATTT
TGGATTATGATACACTTCAAGCTTTACCAAAATTCAAAGAACGTTTGGATTGGTTCGTAA
AGTATAGACCAAATTTAGTGAAAAACATTGCTCCATTCACTGCTTTGGGTCAAAATAAAT
CACGTTTACTCTCAATTGTAAATCGTGAGAAATTGGAATTAATCTTAAAGAAAATGTTGG
ATGAAGATTGGTTCCTCTCTGAAAATGGTATTAGATCATTATCAAAACATCACAAGGATC
ATCCATTCACTTTTGCTGAGATGGATGCTTGTGTTGGTTATGTACCTGGCGAGAGTGATG
TTCATATCATGGGTTCAAATAGTAATTGGAGAGGTCCAGTTTGGTTTGCCGTAAATTATC
TCATGATTCAATCACTTCGTACCTATCATGAATACTATGGTGATGAATTCCAAATTGAAT
ATCCAACAAACTCTGGTAACCTCGTAACTTTGGATATCGTAGCCGATGATATCTCTAAAC
GTCTCATTAGTATCTTCCAAGAGAATCAAGATGGTACTAGAAAATTTGCTGGTGGTAAAC
CAATTTTCGCAAAAAAACATTGGAAAGATCATATATTGTTCAATGAATATTTCCATGGTG
ATAGTGGTGCTGGTCTTGGTGCTTCTCACCAGAC
sequence update 2002.10.25
Translated Amino Acid sequence 
TIIMEQKRLDDSLLRNSDWKNWGTYVSDRQWATVREDYGGEPWTYFPHDHARSRAYRWSE
DSIGGFCNRFQNICLGLAFWNEKDPIIKERFFGLGGPEGNHGEDVKEHYFYLDNTPTHSY
AKMLYKYPMNEYPYSDLVSTNAQRGKQDPEYEIEDTGIFDKDEYFDCYIEYAKGDQEDDI
LCKVTVHNRS---

---FAAEILDYDTLQALPKFKERLDWFVKYRPNLVKNIAPFTALGQNKSRLLSIVNREKL
ELILKKMLDEDWFLSENGIRSLSKHHKDHPFTFAEMDACVGYVPGESDVHIMGSNSNWRG
PVWFAVNYLMIQSLRTYHEYYGDEFQIEYPTNSGNLVTLDIVADDISKRLISIFQENQDG
TRKFAGGKPIFAKKHWKDHILFNEYFHGDSGAGLGASHQ
Translated Amino Acid sequence (All Frames) 
Frame A:
sq**wnkkd*mihy*eiligrigvpm*vidngqlfvkimvvnhgptfhtimldhvhidgq
rivlvdfvidfkifv*v*hfgmkkiqllkrdfldlvdqkeimvkm*kniisi*iihqhip
mpkcyiniq*mnihivi*yqpmhnvvnkiqsmklkilvslirmnisivtlnmlkeikkmi
fyvklpyiiv---

---clllkfwimihfklyqnsknvwigs*sidqi**ktllhsllwvkinhvysql*ivrn
wn*s*rkcwmkigsslkmvldhyqnitriihslllrwmlvlvmylarvmfiswvqivige
vqfglp*iis*fnhfvpimntmvmnsklniqqtlvts*lwis*pmislnvslvsskrikm
vlenllvvnqfsqknigkiiycsmnismvivvlvlvlltr

Frame B:
hnnngtkkir*fiikkf*leelgylck**tmgncs*rlww*tmdllstrsc*itci*mvr
g*ywwil**iskylfrfsile*krsny*reifwtwwtrrksw*rckrtlflfr*ytntfl
cqnvi*isne*isi**fsinqcttw*trsrv*n*rywyl**g*ifrllh*ic*rrsrr*y
fm*syrt*sf---

---vcc*nfgl*ytssftkiqrtfglvrkv*tkfsekhcsihcfgsk*itftlncks*ei
ginlkenvg*rlvpl*kwy*iiiktsqgssihfc*dgclcwlctwre*csyhgfk**ler
sslvcrklshdsitsyls*ilw**ipn*isnklw*prnfgyrsr*yl*tsh*ylpresrw
y*kicww*tnfrkktlersyivq*ifpw**wcwswcfspd

Frame C:
TIIMEQKRLDDSLLRNSDWKNWGTYVSDRQWATVREDYGGEPWTYFPHDHARSRAYRWSE
DSIGGFCNRFQNICLGLAFWNEKDPIIKERFFGLGGPEGNHGEDVKEHYFYLDNTPTHSY
AKMLYKYPMNEYPYSDLVSTNAQRGKQDPEYEIEDTGIFDKDEYFDCYIEYAKGDQEDDI
LCKVTVHNRS---

---FAAEILDYDTLQALPKFKERLDWFVKYRPNLVKNIAPFTALGQNKSRLLSIVNREKL
ELILKKMLDEDWFLSENGIRSLSKHHKDHPFTFAEMDACVGYVPGESDVHIMGSNSNWRG
PVWFAVNYLMIQSLRTYHEYYGDEFQIEYPTNSGNLVTLDIVADDISKRLISIFQENQDG
TRKFAGGKPIFAKKHWKDHILFNEYFHGDSGAGLGASHQ
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL133 (VHL133Q) /CSM/VH/VHL1-B/VHL133Q.Seq.d/                       2345   0.0
VHL253 (VHL253Q) /CSM/VH/VHL2-C/VHL253Q.Seq.d/                       1251   0.0
VHI240 (VHI240Q) /CSM/VH/VHI2-B/VHI240Q.Seq.d/                       1251   0.0
VHD431 (VHD431Q) /CSM/VH/VHD4-B/VHD431Q.Seq.d/                       1251   0.0
VHB280 (VHB280Q) /CSM/VH/VHB2-D/VHB280Q.Seq.d/                       1251   0.0
VHB246 (VHB246Q) /CSM/VH/VHB2-B/VHB246Q.Seq.d/                       1251   0.0
VHA209 (VHA209Q) /CSM/VH/VHA2-A/VHA209Q.Seq.d/                       1251   0.0
AHF215 (AHF215Q) /CSM/AH/AHF2-A/AHF215Q.Seq.d/                       1251   0.0
VHL283 (VHL283Q) /CSM/VH/VHL2-D/VHL283Q.Seq.d/                       1247   0.0
VHP462 (VHP462Q) /CSM/VH/VHP4-C/VHP462Q.Seq.d/                       1243   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427204) Dictyostelium discoideum cDNA clone:ddv61l16, 5' ...  1134   0.0    1
(BJ425677) Dictyostelium discoideum cDNA clone:ddv56a17, 5' ...  1134   0.0    1
(BJ425659) Dictyostelium discoideum cDNA clone:ddv56n09, 5' ...  1134   0.0    1
(BJ424852) Dictyostelium discoideum cDNA clone:ddv53e22, 5' ...  1134   0.0    1
(BJ424793) Dictyostelium discoideum cDNA clone:ddv53i14, 5' ...  1134   0.0    1
(BJ424685) Dictyostelium discoideum cDNA clone:ddv53a09, 5' ...  1134   0.0    1
(BJ422916) Dictyostelium discoideum cDNA clone:ddv47o10, 5' ...  1134   0.0    1
(BJ422449) Dictyostelium discoideum cDNA clone:ddv45o23, 5' ...  1134   0.0    1
(BJ422181) Dictyostelium discoideum cDNA clone:ddv45j04, 5' ...  1134   0.0    1
(BJ422081) Dictyostelium discoideum cDNA clone:ddv44d24, 5' ...  1134   0.0    1
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BX908798_121(BX908798|pid:none) Parachlamydia-related symbiont U...   254   6e-66
AM236080_63(AM236080|pid:none) Rhizobium leguminosarum bv. vicia...   253   1e-65
CP001287_390(CP001287|pid:none) Cyanothece sp. PCC 8801, complet...   252   2e-65
CT573073_696(CT573073|pid:none) Kuenenia stuttgartiensis genome ...   251   5e-65
CP001344_1586(CP001344|pid:none) Cyanothece sp. PCC 7425, comple...   250   8e-65
AY120852_6(AY120852|pid:none) Synechococcus sp. PCC 7942 PacS, d...   249   1e-64
CP001344_4154(CP001344|pid:none) Cyanothece sp. PCC 7425, comple...   248   3e-64
CP000473_7467(CP000473|pid:none) Solibacter usitatus Ellin6076, ...   248   4e-64
CP000698_2591(CP000698|pid:none) Geobacter uraniireducens Rf4, c...   246   9e-64
CP000383_3377(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   246   9e-64
protein update 2009. 7.15
PSORT 

 psg: 0.75  gvh: 0.30  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHL133 is cyt
5' end seq. ID VHL133F
5' end seq. 
>VHL133F.Seq
TCACAATAATAATGGAACAAAAAAGATTAGATGATTCATTATTAAGAAATTCTGATTGGA
AGAATTGGGGTACCTATGTAAGTGATAGACAATGGGCAACTGTTCGTGAAGATTATGGTG
GTGAACCATGGACCTACTTTCCACACGATCATGCTAGATCACGTGCATATAGATGGTCAG
AGGATAGTATTGGTGGATTTTGTAATAGATTTCAAAATATTTGTTTAGGTTTAGCATTTT
GGAATGAAAAAGATCCAATTATTAAAGAGAGATTTTTTGGACTTGGTGGACCAGAAGGAA
ATCATGGTGAAGATGTAAAAGAACATTATTTCTATTTAGATAATACACCAACACATTCCT
ATGCCAAAATGTTATATAAATATCCAATGAATGAATATCCATATAGTGATTTAGTATCAA
CCAATGCACAACGTGGTAAACAAGATCCAGAGTATGAAATTGAAGATACTGGTATCTTTG
ATAAGGATGAATATTTCGATTGTTACATTGAATATGCTAAAGGAGATCAAGAAGATGATA
TTTTATGTAAAGTTACCGTACATAATCGTTCANNNNNNNNNN
Length of 5' end seq. 582
3' end seq. ID VHL133Z
3' end seq. 
>VHL133Z.Seq
NNNNNNNNNNTGTTTGCTGCTGAAATTTTGGATTATGATACACTTCAAGCTTTACCAAAA
TTCAAAGAACGTTTGGATTGGTTCGTAAAGTATAGACCAAATTTAGTGAAAAACATTGCT
CCATTCACTGCTTTGGGTCAAAATAAATCACGTTTACTCTCAATTGTAAATCGTGAGAAA
TTGGAATTAATCTTAAAGAAAATGTTGGATGAAGATTGGTTCCTCTCTGAAAATGGTATT
AGATCATTATCAAAACATCACAAGGATCATCCATTCACTTTTGCTGAGATGGATGCTTGT
GTTGGTTATGTACCTGGCGAGAGTGATGTTCATATCATGGGTTCAAATAGTAATTGGAGA
GGTCCAGTTTGGTTTGCCGTAAATTATCTCATGATTCAATCACTTCGTACCTATCATGAA
TACTATGGTGATGAATTCCAAATTGAATATCCAACAAACTCTGGTAACCTCGTAACTTTG
GATATCGTAGCCGATGATATCTCTAAACGTCTCATTAGTATCTTCCAAGAGAATCAAGAT
GGTACTAGAAAATTTGCTGGTGGTAAACCAATTTTCGCAAAAAAACATTGGAAAGATCAT
ATATTGTTCAATGAATATTTCCATGGTGATAGTGGTGCTGGTCTTGGTGCTTCTCACCAG
AC
Length of 3' end seq. 662
Connected seq. ID VHL133P
Connected seq. 
>VHL133P.Seq
TCACAATAATAATGGAACAAAAAAGATTAGATGATTCATTATTAAGAAATTCTGATTGGA
AGAATTGGGGTACCTATGTAAGTGATAGACAATGGGCAACTGTTCGTGAAGATTATGGTG
GTGAACCATGGACCTACTTTCCACACGATCATGCTAGATCACGTGCATATAGATGGTCAG
AGGATAGTATTGGTGGATTTTGTAATAGATTTCAAAATATTTGTTTAGGTTTAGCATTTT
GGAATGAAAAAGATCCAATTATTAAAGAGAGATTTTTTGGACTTGGTGGACCAGAAGGAA
ATCATGGTGAAGATGTAAAAGAACATTATTTCTATTTAGATAATACACCAACACATTCCT
ATGCCAAAATGTTATATAAATATCCAATGAATGAATATCCATATAGTGATTTAGTATCAA
CCAATGCACAACGTGGTAAACAAGATCCAGAGTATGAAATTGAAGATACTGGTATCTTTG
ATAAGGATGAATATTTCGATTGTTACATTGAATATGCTAAAGGAGATCAAGAAGATGATA
TTTTATGTAAAGTTACCGTACATAATCGTTCA----------TGTTTGCTGCTGAAATTT
TGGATTATGATACACTTCAAGCTTTACCAAAATTCAAAGAACGTTTGGATTGGTTCGTAA
AGTATAGACCAAATTTAGTGAAAAACATTGCTCCATTCACTGCTTTGGGTCAAAATAAAT
CACGTTTACTCTCAATTGTAAATCGTGAGAAATTGGAATTAATCTTAAAGAAAATGTTGG
ATGAAGATTGGTTCCTCTCTGAAAATGGTATTAGATCATTATCAAAACATCACAAGGATC
ATCCATTCACTTTTGCTGAGATGGATGCTTGTGTTGGTTATGTACCTGGCGAGAGTGATG
TTCATATCATGGGTTCAAATAGTAATTGGAGAGGTCCAGTTTGGTTTGCCGTAAATTATC
TCATGATTCAATCACTTCGTACCTATCATGAATACTATGGTGATGAATTCCAAATTGAAT
ATCCAACAAACTCTGGTAACCTCGTAACTTTGGATATCGTAGCCGATGATATCTCTAAAC
GTCTCATTAGTATCTTCCAAGAGAATCAAGATGGTACTAGAAAATTTGCTGGTGGTAAAC
CAATTTTCGCAAAAAAACATTGGAAAGATCATATATTGTTCAATGAATATTTCCATGGTG
ATAGTGGTGCTGGTCTTGGTGCTTCTCACCAGAC
Length of connected seq. 1224
Full length Seq ID -
Full length Seq. -
Length of full length seq. -