NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL123
Library VH
(Link to library)
Clone ID VHL123
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHL123P
(Link to Original site)
Representative DNA sequence 
>VHL123 (VHL123Q) /CSM/VH/VHL1-A/VHL123Q.Seq.d/
TAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCCCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATXXXXXXXXXXTCCAAAGATG
ATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCA
TGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCA
AAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCA
CCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATG
TCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTC
GTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAG
TAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTA
GAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTC
ATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTATGTTCCCACACTGCCGGTC
AAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATA
AGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCC
CAGATCTNACAAATTCCACGAAAAACTCTAAACAATTAAGTCGCACTTNAGTTTTCAAAN
AAGAATCAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
*LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVPERIKPVLFVNKVDRFL---

---SKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFV
GAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTL
LEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADL
CSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLTNSTKNSKQLS
RTXVFKXESN
Translated Amino Acid sequence (All Frames) 
Frame A:
*LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVPERIKPVLFVNKVDRFL---

---SKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFV
GAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTL
LEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADL
CSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLTNSTKNSKQLS
RTXVFKXESN

Frame B:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy---

---pkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilsl
vlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqls
*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliy
vptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqixqiprktlnn*v
alxfsxknq

Frame C:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrp*tyqtslirqqsrsfl---

---qr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrw
cfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnsl
rtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*fm
fphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprsxkfhekl*tiks
hxsfqxrik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL123 (VHL123Q) /CSM/VH/VHL1-A/VHL123Q.Seq.d/                       2401   0.0
VHN393 (VHN393Q) /CSM/VH/VHN3-D/VHN393Q.Seq.d/                       1443   0.0
VHN141 (VHN141Q) /CSM/VH/VHN1-B/VHN141Q.Seq.d/                       1443   0.0
VHM754 (VHM754Q) /CSM/VH/VHM7-C/VHM754Q.Seq.d/                       1443   0.0
VHN240 (VHN240Q) /CSM/VH/VHN2-B/VHN240Q.Seq.d/                       1441   0.0
VHN489 (VHN489Q) /CSM/VH/VHN4-D/VHN489Q.Seq.d/                       1437   0.0
VHM135 (VHM135Q) /CSM/VH/VHM1-B/VHM135Q.Seq.d/                       1437   0.0
VHM635 (VHM635Q) /CSM/VH/VHM6-B/VHM635Q.Seq.d/                       1435   0.0
VHM571 (VHM571Q) /CSM/VH/VHM5-C/VHM571Q.Seq.d/                       1435   0.0
VHN474 (VHN474Q) /CSM/VH/VHN4-D/VHN474Q.Seq.d/                       1429   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ443517) Dictyostelium discoideum cDNA clone:ddv53m05, 3' ...  1451   0.0    1
(BJ444922) Dictyostelium discoideum cDNA clone:ddv57j23, 3' ...  1443   0.0    1
(BJ444713) Dictyostelium discoideum cDNA clone:ddv57a11, 3' ...  1443   0.0    1
(BJ444522) Dictyostelium discoideum cDNA clone:ddv56l13, 3' ...  1443   0.0    1
(BJ444785) Dictyostelium discoideum cDNA clone:ddv57o10, 3' ...  1441   0.0    1
(BJ444890) Dictyostelium discoideum cDNA clone:ddv57b24, 3' ...  1437   0.0    1
(BJ444115) Dictyostelium discoideum cDNA clone:ddv55e09, 3' ...  1437   0.0    1
(BJ444531) Dictyostelium discoideum cDNA clone:ddv56m17, 3' ...  1435   0.0    1
(BJ444412) Dictyostelium discoideum cDNA clone:ddv56e10, 3' ...  1435   0.0    1
(BJ444897) Dictyostelium discoideum cDNA clone:ddv57d20, 3' ...  1429   0.0    1
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   293   1e-77
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   292   2e-77
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   288   4e-76
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   288   4e-76
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   288   4e-76
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   288   4e-76
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   287   6e-76
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   286   1e-75
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   286   1e-75
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   286   1e-75
protein update 2009. 7.15
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHL123 is cyt
5' end seq. ID VHL123F
5' end seq. 
>VHL123F.Seq
TAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCCCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATNNNNNNNNNN
Length of 5' end seq. 530
3' end seq. ID VHL123Z
3' end seq. 
>VHL123Z.Seq
NNNNNNNNNNTCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGA
ATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAA
TCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGT
TGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTAT
CCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCA
AATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCT
CTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTA
CTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCC
ATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTT
ATGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAAT
TGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAA
AGGTTTAGCCCCAGAAATCCCAGATCTNACAAATTCCACGAAAAACTCTAAACAATTAAG
TCGCACTTNAGTTTTCAAANAAGAATCAAAT
Length of 3' end seq. 751
Connected seq. ID VHL123P
Connected seq. 
>VHL123P.Seq
TAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCCCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTAT----------TCCAAAGATG
ATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCA
TGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCA
AAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCA
CCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATG
TCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTC
GTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAG
TAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTA
GAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTC
ATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTATGTTCCCACACTGCCGGTC
AAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATA
AGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCC
CAGATCTNACAAATTCCACGAAAAACTCTAAACAATTAAGTCGCACTTNAGTTTTCAAAN
AAGAATCAAAT
Length of connected seq. 1261
Full length Seq ID -
Full length Seq. -
Length of full length seq. -