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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL116
Library VH
(Link to library)
Clone ID VHL116
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16306-1
Original site URL
Representative seq. ID VHL116P
(Link to Original site)
Representative DNA sequence 
>VHL116 (VHL116Q) /CSM/VH/VHL1-A/VHL116Q.Seq.d/
AAATAAAAAAAAAACAAATCAAATTTTATTTAAATAATATAAAAAAATAAATAATATAAT
ATAAAAAATGATATCATCTCCAATTACCTCATGTACTGAGGCAATTGAAATGATCTCAAA
ACTTCCACAAGAAGTATTTGAAAAAAGTACAAGAACAGCAACAAAGAAATTATTANCATC
AATTGGTTTAGTGACAATTGGTACATATTTAGTATATGCACTTCCATGGTATTTGTTACC
AATTGGATGGATATTTATGGGTACAGCATGTTGNGGATTATTCTCTGTTGGGTATGCATG
TGGTAATGATTTATTTTTCAAGAATAAATTTGTTAACTATGTAGTTGGAACAATTTGTAT
GTTACCATTAATGTATCCATTANCCTATTGGAANAAAAGAATTGAAAAGAAAGCAACGGC
AAGTCGTGATTTCGTTCGTGANATTGCAAGTGGTAATTTTTGGTTCCTCAGTTCATTACT
TCAATGGTTATCATTAAATTTCAGTTTCAAATTCTCCAAATCAATGATGGTCTCAGTCTC
ATTCCTTTATGTATTCATCGCATTATTCTTCCCAATTATGACCTACAGTATGGGCATTTG
GXXXXXXXXXXTACGTTTCCCAACATAAATATTTCATTCCATTGGCATTCAGTTTGGGTT
GGTTCTTACCAATGTTCATTTGTGGTTTTGGTTGGAATGATTGGAAAGGTGGATTTTTCA
TCGCTGGTGTTGCCTCCAAAGTACTCATGATGCAATGTACCTTTTGTATCAACTCTTTGG
CTCATTATCTTGGTGAAGCAACTTACACCGACCAAAGATCTCCACGTGACTCTTTTATCA
CATCTTTGGTCACTTTTGGTGAAGGTTATCATAACTTTCATCATGAATTCCCATATGATT
ACAGAAATGGTATTCATATGTCTGCTTACGATCCAGGTAAGTGGTTAATCAATTTCCTTA
GTTGGTTTGGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTA
AAATTCAAATGCAAGAGAAAACCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAAC
CATTGGATCAATTACCATCATTCACTCGTGCTCAAGTTAAAGAGAAATGTCAAAAAGAAA
AGAAACAACTCTTAATCATTGAAGATGTTATCTATGATATGTCTGAATTTGCTGAAAAAC
ATCCAGGTGGTAAACAATACATTGACGATTATATTGGTAAAGATGCAACTAAAGCTTTTA
ATGGTTTAGTTTAGAATCATTCATTTGCTGCTAGAAATTATTGGATCTTTATAGAGT
sequence update 2002.10.25
Translated Amino Acid sequence 
nkkktnqilfk*ykkinnii*KMISSPITSCTEAIEMISKLPQEVFEKSTRTATKKLLXS
IGLVTIGTYLVYALPWYLLPIGWIFMGTACXGLFSVGYACGNDLFFKNKFVNYVVGTICM
LPLMYPLXYWXKRIEKKATASRDFVRXIASGNFWFLSSLLQWLSLNFSFKFSKSMMVSVS
FLYVFIALFFPIMTYSMGIW---

---YVSQHKYFIPLAFSLGWFLPMFICGFGWNDWKGGFFIAGVASKVLMMQCTFCINSLA
HYLGEATYTDQRSPRDSFITSLVTFGEGYHNFHHEFPYDYRNGIHMSAYDPGKWLINFLS
WFGFAYELKRFPSELFAKGKIQMQEKTIKKLRDQLYWGKPLDQLPSFTRAQVKEKCQKEK
KQLLIIEDVIYDMSEFAEKHPGGKQYIDDYIGKDATKAFNGLV*nhsfaarnywifie
Translated Amino Acid sequence (All Frames) 
Frame A:
k*kknksnfi*ii*knk*ynikndiisnylmy*gn*ndlktstrsi*kkyknsnkeiixi
nwfsdnwyifsictsmvfvtnwmdiygysmlxiilcwvcmw**fifqe*ic*lcswnnly
vtinvsixllexkn*kesngks*frs*xckw*flvpqfitsmviikfqfqilqindglsl
iplcihriilpnydlqyghl---

---YVSQHKYFIPLAFSLGWFLPMFICGFGWNDWKGGFFIAGVASKVLMMQCTFCINSLA
HYLGEATYTDQRSPRDSFITSLVTFGEGYHNFHHEFPYDYRNGIHMSAYDPGKWLINFLS
WFGFAYELKRFPSELFAKGKIQMQEKTIKKLRDQLYWGKPLDQLPSFTRAQVKEKCQKEK
KQLLIIEDVIYDMSEFAEKHPGGKQYIDDYIGKDATKAFNGLV*nhsfaarnywifie

Frame B:
nkkktnqilfk*ykkinnii*KMISSPITSCTEAIEMISKLPQEVFEKSTRTATKKLLXS
IGLVTIGTYLVYALPWYLLPIGWIFMGTACXGLFSVGYACGNDLFFKNKFVNYVVGTICM
LPLMYPLXYWXKRIEKKATASRDFVRXIASGNFWFLSSLLQWLSLNFSFKFSKSMMVSVS
FLYVFIALFFPIMTYSMGIW---

---tfpninisfhwhsvwvgsyqcsfvvlvgmigkvdfsslvlppkys*cnvpfvstlwl
iilvkqltptkdlhvtllshlwsllvkviitfimnshmitemvficlltiqvsg*sislv
glvspmn*rdsqvnysqkvkfkckrkpsknyvinsigvnhwinyhhslvlklkrnvkkkr
nns*slkmlsmiclnllkniqvvnntltiilvkmqlkllmv*friihllleiigsl*s

Frame C:
ikkkqikfylnnikk*ii*ykk*yhlqlphvlrqlk*sqnfhkkylkkvqeqqqrnyxhq
lv**qlvhi*ymhfhgicyqldgylwvqhvxdysllgmhvvmiyfsrinlltm*leqfvc
yh*cihxpigxkelkrkqrqvvisfvxlqvvifgssvhyfngyh*isvsnspnq*wsqsh
sfmysshyssql*ptvwaf---

---rfpt*ifhsigiqfglvltnvhlwfwle*lerwifhrwcclqsthdamyllyqlfgs
lsw*snlhrpkist*lfyhifghfw*rls*lss*ipi*lqkwysyvclrsr*vvnqfp*l
vwfrl*ikeipk*iirkr*nsnarenhqkit*stllg*tigsitiihscss*remskrke
ttlnh*rcyl*yv*ic*ktsrw*tih*rlyw*rcn*sf*wfslesficc*klldlyr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL116 (VHL116Q) /CSM/VH/VHL1-A/VHL116Q.Seq.d/                       2349   0.0
VHK569 (VHK569Q) /CSM/VH/VHK5-C/VHK569Q.Seq.d/                       1378   0.0
VHD339 (VHD339Q) /CSM/VH/VHD3-B/VHD339Q.Seq.d/                       1372   0.0
VHM540 (VHM540Q) /CSM/VH/VHM5-B/VHM540Q.Seq.d/                       1368   0.0
SFI188 (SFI188Q) /CSM/SF/SFI1-D/SFI188Q.Seq.d/                       1364   0.0
VHO360 (VHO360Q) /CSM/VH/VHO3-C/VHO360Q.Seq.d/                       1362   0.0
VHD238 (VHD238Q) /CSM/VH/VHD2-B/VHD238Q.Seq.d/                       1362   0.0
VHC619 (VHC619Q) /CSM/VH/VHC6-A/VHC619Q.Seq.d/                       1362   0.0
VHB877 (VHB877Q) /CSM/VH/VHB8-D/VHB877Q.Seq.d/                       1362   0.0
VHA359 (VHA359Q) /CSM/VH/VHA3-C/VHA359Q.Seq.d/                       1362   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ443524) Dictyostelium discoideum cDNA clone:ddv53o03, 3' ...  1400   0.0    1
(BJ443335) Dictyostelium discoideum cDNA clone:ddv52i17, 3' ...  1362   0.0    2
(BJ438605) Dictyostelium discoideum cDNA clone:ddv37n09, 3' ...  1356   0.0    2
(BJ445481) Dictyostelium discoideum cDNA clone:ddv59h15, 3' ...  1346   0.0    2
(BJ438590) Dictyostelium discoideum cDNA clone:ddv37k10, 3' ...  1346   0.0    2
(BJ438003) Dictyostelium discoideum cDNA clone:ddv36e06, 3' ...  1346   0.0    2
(BJ437632) Dictyostelium discoideum cDNA clone:ddv34j20, 3' ...  1346   0.0    2
(BJ436568) Dictyostelium discoideum cDNA clone:ddv31f15, 3' ...  1346   0.0    2
(BJ436541) Dictyostelium discoideum cDNA clone:ddv31a16, 3' ...  1346   0.0    2
(BJ386216) Dictyostelium discoideum cDNA clone:ddc57c14, 3' ...  1346   0.0    2
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY995173_1(AY995173|pid:none) Amylomyces rouxii delta-9 desatura...   243   1e-62
AB196774_1(AB196774|pid:none) Mortierella alpina gene for omega9...   227   6e-58
AF085500_1(AF085500|pid:none) Mortierella alpina delta nine desa...   226   2e-57
Y18553_1(Y18553|pid:none) Mortierella alpina ole1 gene for stear...   225   2e-57
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   224   5e-57
AB195980_1(AB195980|pid:none) Mortierella alpina gene for omega9...   223   2e-56
AP007151_392(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   218   4e-55
AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   214   6e-54
AY576801_1(AY576801|pid:none) Hortaea werneckii putative delta-9...   214   7e-54
AM920435_140(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   213   9e-54
protein update 2009. 7.15
PSORT 

 psg: 0.88  gvh: 0.50  alm: 0.24  top: 0.47  tms: 0.33  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: endoplasmic reticulum
	 24.0 %: plasma membrane
	 20.0 %: mitochondrial
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system

>> prediction for VHL116 is end
5' end seq. ID VHL116F
5' end seq. 
>VHL116F.Seq
AAATAAAAAAAAAACAAATCAAATTTTATTTAAATAATATAAAAAAATAAATAATATAAT
ATAAAAAATGATATCATCTCCAATTACCTCATGTACTGAGGCAATTGAAATGATCTCAAA
ACTTCCACAAGAAGTATTTGAAAAAAGTACAAGAACAGCAACAAAGAAATTATTANCATC
AATTGGTTTAGTGACAATTGGTACATATTTAGTATATGCACTTCCATGGTATTTGTTACC
AATTGGATGGATATTTATGGGTACAGCATGTTGNGGATTATTCTCTGTTGGGTATGCATG
TGGTAATGATTTATTTTTCAAGAATAAATTTGTTAACTATGTAGTTGGAACAATTTGTAT
GTTACCATTAATGTATCCATTANCCTATTGGAANAAAAGAATTGAAAAGAAAGCAACGGC
AAGTCGTGATTTCGTTCGTGANATTGCAAGTGGTAATTTTTGGTTCCTCAGTTCATTACT
TCAATGGTTATCATTAAATTTCAGTTTCAAATTCTCCAAATCAATGATGGTCTCAGTCTC
ATTCCTTTATGTATTCATCGCATTATTCTTCCCAATTATGACCTACAGTATGGGCATTTG
GNNNNNNNNNN
Length of 5' end seq. 611
3' end seq. ID VHL116Z
3' end seq. 
>VHL116Z.Seq
NNNNNNNNNNTACGTTTCCCAACATAAATATTTCATTCCATTGGCATTCAGTTTGGGTTG
GTTCTTACCAATGTTCATTTGTGGTTTTGGTTGGAATGATTGGAAAGGTGGATTTTTCAT
CGCTGGTGTTGCCTCCAAAGTACTCATGATGCAATGTACCTTTTGTATCAACTCTTTGGC
TCATTATCTTGGTGAAGCAACTTACACCGACCAAAGATCTCCACGTGACTCTTTTATCAC
ATCTTTGGTCACTTTTGGTGAAGGTTATCATAACTTTCATCATGAATTCCCATATGATTA
CAGAAATGGTATTCATATGTCTGCTTACGATCCAGGTAAGTGGTTAATCAATTTCCTTAG
TTGGTTTGGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTAA
AATTCAAATGCAAGAGAAAACCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAACC
ATTGGATCAATTACCATCATTCACTCGTGCTCAAGTTAAAGAGAAATGTCAAAAAGAAAA
GAAACAACTCTTAATCATTGAAGATGTTATCTATGATATGTCTGAATTTGCTGAAAAACA
TCCAGGTGGTAAACAATACATTGACGATTATATTGGTAAAGATGCAACTAAAGCTTTTAA
TGGTTTAGTTTAGAATCATTCATTTGCTGCTAGAAATTATTGGATCTTTATAGAGT
Length of 3' end seq. 716
Connected seq. ID VHL116P
Connected seq. 
>VHL116P.Seq
AAATAAAAAAAAAACAAATCAAATTTTATTTAAATAATATAAAAAAATAAATAATATAAT
ATAAAAAATGATATCATCTCCAATTACCTCATGTACTGAGGCAATTGAAATGATCTCAAA
ACTTCCACAAGAAGTATTTGAAAAAAGTACAAGAACAGCAACAAAGAAATTATTANCATC
AATTGGTTTAGTGACAATTGGTACATATTTAGTATATGCACTTCCATGGTATTTGTTACC
AATTGGATGGATATTTATGGGTACAGCATGTTGNGGATTATTCTCTGTTGGGTATGCATG
TGGTAATGATTTATTTTTCAAGAATAAATTTGTTAACTATGTAGTTGGAACAATTTGTAT
GTTACCATTAATGTATCCATTANCCTATTGGAANAAAAGAATTGAAAAGAAAGCAACGGC
AAGTCGTGATTTCGTTCGTGANATTGCAAGTGGTAATTTTTGGTTCCTCAGTTCATTACT
TCAATGGTTATCATTAAATTTCAGTTTCAAATTCTCCAAATCAATGATGGTCTCAGTCTC
ATTCCTTTATGTATTCATCGCATTATTCTTCCCAATTATGACCTACAGTATGGGCATTTG
G----------TACGTTTCCCAACATAAATATTTCATTCCATTGGCATTCAGTTTGGGTT
GGTTCTTACCAATGTTCATTTGTGGTTTTGGTTGGAATGATTGGAAAGGTGGATTTTTCA
TCGCTGGTGTTGCCTCCAAAGTACTCATGATGCAATGTACCTTTTGTATCAACTCTTTGG
CTCATTATCTTGGTGAAGCAACTTACACCGACCAAAGATCTCCACGTGACTCTTTTATCA
CATCTTTGGTCACTTTTGGTGAAGGTTATCATAACTTTCATCATGAATTCCCATATGATT
ACAGAAATGGTATTCATATGTCTGCTTACGATCCAGGTAAGTGGTTAATCAATTTCCTTA
GTTGGTTTGGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTA
AAATTCAAATGCAAGAGAAAACCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAAC
CATTGGATCAATTACCATCATTCACTCGTGCTCAAGTTAAAGAGAAATGTCAAAAAGAAA
AGAAACAACTCTTAATCATTGAAGATGTTATCTATGATATGTCTGAATTTGCTGAAAAAC
ATCCAGGTGGTAAACAATACATTGACGATTATATTGGTAAAGATGCAACTAAAGCTTTTA
ATGGTTTAGTTTAGAATCATTCATTTGCTGCTAGAAATTATTGGATCTTTATAGAGT
Length of connected seq. 1307
Full length Seq ID -
Full length Seq. -
Length of full length seq. -