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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHL102
Library VH
(Link to library)
Clone ID VHL102
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U16415-1
Original site URL
Representative seq. ID VHL102P
(Link to Original site)
Representative DNA sequence 
>VHL102 (VHL102Q) /CSM/VH/VHL1-A/VHL102Q.Seq.d/
ATTTTTAAAAAATGGCAACAATTGAAGATACTTNAAAGATAATAACAATCCAATTTTAGA
ACGTAAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGATGATAATTCAG
TAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGTGACACACTTT
TAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGATCCAACCATCG
ATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTTAGATTAGGTG
ACATGATTTCAGTTCACCAATGTTCAGGTGTTAAATATGGCAAACGTATTCATGTGTTAC
CAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTACCTCAAACCAT
ACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTACGTGGTGGTA
TGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGTATCGTTGCAC
CAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAGAGGATGAAGACCGTCTCG
ATGAGXXXXXXXXXXACCATGTGGTTTGGTGAGTCTGAAGCCAACGTTCGTGAACTCTTT
GATAAAGCTCGTCAAGCCGCTCCATGTGTTTTGTTCTTTGATGAGTTGGATAGTATTGCA
CGTTCTAGAGGTTCATCACAGGGTGATGCTGGTGGTGCTGGTGATAGAGTTATCAATCAA
ATTTTAACTGAAATGGATGGTATGAACGCCAAGAAGAACGTTTTCATCATTGGTGCTACC
AATAGACCAGATATCATCGATCCAGCTATCCTCCGTCCAGGTCGTTTAGATCAACTCATT
TACATTCCATTACCAGATTTACCATCTCGTGTTGCCATTCTCAAGGCTTGTCTCAATAAA
TCACCAGTCGCCAAGGATGTAGATTTAGAATTTTTGGGTCAAAAGACTCAAGGTTTCTCT
GGTGCAGATTTAACTGAAATTTGTCAACGTGCCTGTAAATTAGCTATTCGTGAATCCATT
GAAAAGGATATTGAATCCACCAAAGCCCGTCAAGAATCTGGTGATACCAAGATGGAAGAT
GACTCTGTCGATCCAGTACCAGAAATTACTCGTGATCACTTCCAAGAAGCCATGAGATCT
GCTCGTCGTTCTGTATCCGATAATGATATTAGAAAATATGAAAGCTTTGCTCAAACTTTA
GTTCAATCAAGAGGTCTGGTAATAATTTCAAATTCCCAGATCAAGAATCTTCAGGTCAAT
TCAATCAAGATCAATCNAGATCTTTCAG
sequence update 2002.10.25
Translated Amino Acid sequence 
f*kmatiedtxkiitiqf*NVKKAPNRLFVEEAINDDNSVVTLNPETMDQLQFFRGDTLL
IKGKKRRDTVCIVLSDPTIDPSKIRMNKVVRNNLRVRLGDMISVHQCSGVKYGKRIHVLP
IDDTIEGLSGNLFDLYLKPYFLEAYRPVRKGDLFLVRGGMRAVEFKVVETDPGEYCIVAP
ETFIHCEGEAVKREDEDRLDE---

---TMWFGESEANVRELFDKARQAAPCVLFFDELDSIARSRGSSQGDAGGAGDRVINQIL
TEMDGMNAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDLPSRVAILKACLNKSP
VAKDVDLEFLGQKTQGFSGADLTEICQRACKLAIRESIEKDIESTKARQESGDTKMEDDS
VDPVPEITRDHFQEAMRSARRSVSDNDIRKYESFAQTLVQSRGLVIISNSQIKNLQVNSI
KINXDLS
Translated Amino Acid sequence (All Frames) 
Frame A:
ifkkwqqlkilxr**qsnfrt*kklqidyl*kkqlmmiiq*lh*iqrqwinynslevthf
*lkvkreeipfvlycqiqpsihqrsv*ikl*eiifvld*vt*fqftnvqvlnmanvfmcy
qlmip*kvsvaisliftsnhtf*kptvqsvrvtyf*yvvv*ep*nlklskptqvnivslh
qrhlyivkvrqsrermktvsm---

---TMWFGESEANVRELFDKARQAAPCVLFFDELDSIARSRGSSQGDAGGAGDRVINQIL
TEMDGMNAKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDLPSRVAILKACLNKSP
VAKDVDLEFLGQKTQGFSGADLTEICQRACKLAIRESIEKDIESTKARQESGDTKMEDDS
VDPVPEITRDHFQEAMRSARRSVSDNDIRKYESFAQTLVQSRGLVIISNSQIKNLQVNSI
KINXDLS

Frame B:
flkngnn*ryxkdnnnpilerkkssk*iicrrsn****fssyiksrdngsitil*r*htf
n*r*kekryrlycivrsnhrsikdpye*sckk*ssc*ir*hdfsspmfrc*iwqtyscvt
n**yhrrsqwqsl*slpqtilfrslpssp*g*lifstwwyesrri*scrnrpr*ilyrct
rdiytl*r*gsqerg*rpsr*---

---pcglvslkptfvnsliklvkplhvfcslmswivlhvlevhhrvmlvvlvielsikf*
lkwmv*tprrtfsslvlpidqissiqlssvqvv*insftfhyqiyhlvlpfsrlvsinhq
sprm*i*nfwvkrlkvslvqi*lkfvnvpvn*lfvnplkrilnppkpvknlviprwkmtl
siqyqkllvitskkp*dllvvlypimilenmkallkl*fnqevw**fqiprsrifrsiqs
rsixifq

Frame C:
f*kmatiedtxkiitiqf*NVKKAPNRLFVEEAINDDNSVVTLNPETMDQLQFFRGDTLL
IKGKKRRDTVCIVLSDPTIDPSKIRMNKVVRNNLRVRLGDMISVHQCSGVKYGKRIHVLP
IDDTIEGLSGNLFDLYLKPYFLEAYRPVRKGDLFLVRGGMRAVEFKVVETDPGEYCIVAP
ETFIHCEGEAVKREDEDRLDE---

---hvvw*v*sqrs*tl**ssssrsmcfvl**vg*yctf*rfitg*cwwcw**syqsnfn
*ngwyerqeerfhhwcyq*tryhrssyppsrsfrsthlhsitrftiscchsqglsq*its
rqgcrfrifgskdsrflwcrfn*nlstcl*isys*ih*kgy*ihqspsriw*yqdgr*lc
rsstrnys*slprsheicssfcir**y*ki*klcsnfssikrsgnnfkfpdqessgqfnq
dqsrsf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHL102 (VHL102Q) /CSM/VH/VHL1-A/VHL102Q.Seq.d/                       2559   0.0
AFG276 (AFG276Q) /CSM/AF/AFG2-D/AFG276Q.Seq.d/                       1388   0.0
VHP286 (VHP286Q) /CSM/VH/VHP2-D/VHP286Q.Seq.d/                       1378   0.0
CHI521 (CHI521Q) /CSM/CH/CHI5-A/CHI521Q.Seq.d/                       1378   0.0
AHF140 (AHF140Q) /CSM/AH/AHF1-B/AHF140Q.Seq.d/                       1378   0.0
AHE182 (AHE182Q) /CSM/AH/AHE1-D/AHE182Q.Seq.d/                       1378   0.0
AHJ267 (AHJ267Q) /CSM/AH/AHJ2-C/AHJ267Q.Seq.d/                       1374   0.0
AHE345 (AHE345Q) /CSM/AH/AHE3-B/AHE345Q.Seq.d/                       1370   0.0
AHL742 (AHL742Q) /CSM/AH/AHL7-B/AHL742Q.Seq.d/                       1362   0.0
AHC203 (AHC203Q) /CSM/AH/AHC2-A/AHC203Q.Seq.d/                       1356   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ443466) Dictyostelium discoideum cDNA clone:ddv53c01, 3' ...  1447   0.0    1
(BJ398143) Dictyostelium discoideum cDNA clone:dds11n06, 3' ...  1342   0.0    1
(BJ342400) Dictyostelium discoideum cDNA clone:dda13g20, 3' ...  1342   0.0    2
(U83085) Dictyostelium discoideum cell division cycle protei...  1304   0.0    2
(BJ446173) Dictyostelium discoideum cDNA clone:ddv61k22, 3' ...  1304   0.0    3
(BJ380729) Dictyostelium discoideum cDNA clone:ddc39i05, 3' ...  1304   0.0    3
(BJ350922) Dictyostelium discoideum cDNA clone:dda41o09, 3' ...  1304   0.0    3
(BJ350407) Dictyostelium discoideum cDNA clone:dda39c21, 3' ...  1304   0.0    3
(BJ354640) Dictyostelium discoideum cDNA clone:dda54d11, 3' ...  1302   0.0    3
(BJ353270) Dictyostelium discoideum cDNA clone:dda49e18, 3' ...  1300   0.0    3
dna update 2008.11.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83085_1(U83085|pid:none) Dictyostelium discoideum cell division...   397   e-116
AK167794_1(AK167794|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   322   2e-86
AK083821_1(AK083821|pid:none) Mus musculus 12 days embryo spinal...   322   2e-86
S25197(S25197;S30329) transitional endoplasmic reticulum ATPase ...   322   2e-86
BC122550_1(BC122550|pid:none) Homo sapiens valosin-containing pr...   322   2e-86
AL137377_1(AL137377|pid:none) Homo sapiens mRNA; cDNA DKFZp434K0...   322   2e-86
(P03974) RecName: Full=Transitional endoplasmic reticulum ATPase...   322   2e-86
AK169140_1(AK169140|pid:none) Mus musculus 17 days embryo kidney...   322   2e-86
(P55072) RecName: Full=Transitional endoplasmic reticulum ATPase...   322   2e-86
(Q3ZBT1) RecName: Full=Transitional endoplasmic reticulum ATPase...   322   2e-86
protein update 2009. 7.15
PSORT 

 psg: 0.68  gvh: 0.33  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.03
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHL102 is cyt
5' end seq. ID VHL102F
5' end seq. 
>VHL102F.Seq
ATTTTTAAAAAATGGCAACAATTGAAGATACTTNAAAGATAATAACAATCCAATTTTAGA
ACGTAAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGATGATAATTCAG
TAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGTGACACACTTT
TAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGATCCAACCATCG
ATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTTAGATTAGGTG
ACATGATTTCAGTTCACCAATGTTCAGGTGTTAAATATGGCAAACGTATTCATGTGTTAC
CAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTACCTCAAACCAT
ACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTACGTGGTGGTA
TGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGTATCGTTGCAC
CAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAGAGGATGAAGACCGTCTCG
ATGAGNNNNNNNNNN
Length of 5' end seq. 615
3' end seq. ID VHL102Z
3' end seq. 
>VHL102Z.Seq
NNNNNNNNNNACCATGTGGTTTGGTGAGTCTGAAGCCAACGTTCGTGAACTCTTTGATAA
AGCTCGTCAAGCCGCTCCATGTGTTTTGTTCTTTGATGAGTTGGATAGTATTGCACGTTC
TAGAGGTTCATCACAGGGTGATGCTGGTGGTGCTGGTGATAGAGTTATCAATCAAATTTT
AACTGAAATGGATGGTATGAACGCCAAGAAGAACGTTTTCATCATTGGTGCTACCAATAG
ACCAGATATCATCGATCCAGCTATCCTCCGTCCAGGTCGTTTAGATCAACTCATTTACAT
TCCATTACCAGATTTACCATCTCGTGTTGCCATTCTCAAGGCTTGTCTCAATAAATCACC
AGTCGCCAAGGATGTAGATTTAGAATTTTTGGGTCAAAAGACTCAAGGTTTCTCTGGTGC
AGATTTAACTGAAATTTGTCAACGTGCCTGTAAATTAGCTATTCGTGAATCCATTGAAAA
GGATATTGAATCCACCAAAGCCCGTCAAGAATCTGGTGATACCAAGATGGAAGATGACTC
TGTCGATCCAGTACCAGAAATTACTCGTGATCACTTCCAAGAAGCCATGAGATCTGCTCG
TCGTTCTGTATCCGATAATGATATTAGAAAATATGAAAGCTTTGCTCAAACTTTAGTTCA
ATCAAGAGGTCTGGTAATAATTTCAAATTCCCAGATCAAGAATCTTCAGGTCAATTCAAT
CAAGATCAATCNAGATCTTTCAG
Length of 3' end seq. 743
Connected seq. ID VHL102P
Connected seq. 
>VHL102P.Seq
ATTTTTAAAAAATGGCAACAATTGAAGATACTTNAAAGATAATAACAATCCAATTTTAGA
ACGTAAAAAAAGCTCCAAATAGATTATTTGTAGAAGAAGCAATTAATGATGATAATTCAG
TAGTTACATTAAATCCAGAGACAATGGATCAATTACAATTCTTTAGAGGTGACACACTTT
TAATTAAAGGTAAAAAGAGAAGAGATACCGTTTGTATTGTATTGTCAGATCCAACCATCG
ATCCATCAAAGATCCGTATGAATAAAGTTGTAAGAAATAATCTTCGTGTTAGATTAGGTG
ACATGATTTCAGTTCACCAATGTTCAGGTGTTAAATATGGCAAACGTATTCATGTGTTAC
CAATTGATGATACCATAGAAGGTCTCAGTGGCAATCTCTTTGATCTTTACCTCAAACCAT
ACTTTTTAGAAGCCTACCGTCCAGTCCGTAAGGGTGACTTATTTTTAGTACGTGGTGGTA
TGAGAGCCGTAGAATTTAAAGTTGTCGAAACCGACCCAGGTGAATATTGTATCGTTGCAC
CAGAGACATTTATACATTGTGAAGGTGAGGCAGTCAAGAGAGAGGATGAAGACCGTCTCG
ATGAG----------ACCATGTGGTTTGGTGAGTCTGAAGCCAACGTTCGTGAACTCTTT
GATAAAGCTCGTCAAGCCGCTCCATGTGTTTTGTTCTTTGATGAGTTGGATAGTATTGCA
CGTTCTAGAGGTTCATCACAGGGTGATGCTGGTGGTGCTGGTGATAGAGTTATCAATCAA
ATTTTAACTGAAATGGATGGTATGAACGCCAAGAAGAACGTTTTCATCATTGGTGCTACC
AATAGACCAGATATCATCGATCCAGCTATCCTCCGTCCAGGTCGTTTAGATCAACTCATT
TACATTCCATTACCAGATTTACCATCTCGTGTTGCCATTCTCAAGGCTTGTCTCAATAAA
TCACCAGTCGCCAAGGATGTAGATTTAGAATTTTTGGGTCAAAAGACTCAAGGTTTCTCT
GGTGCAGATTTAACTGAAATTTGTCAACGTGCCTGTAAATTAGCTATTCGTGAATCCATT
GAAAAGGATATTGAATCCACCAAAGCCCGTCAAGAATCTGGTGATACCAAGATGGAAGAT
GACTCTGTCGATCCAGTACCAGAAATTACTCGTGATCACTTCCAAGAAGCCATGAGATCT
GCTCGTCGTTCTGTATCCGATAATGATATTAGAAAATATGAAAGCTTTGCTCAAACTTTA
GTTCAATCAAGAGGTCTGGTAATAATTTCAAATTCCCAGATCAAGAATCTTCAGGTCAAT
TCAATCAAGATCAATCNAGATCTTTCAG
Length of connected seq. 1338
Full length Seq ID -
Full length Seq. -
Length of full length seq. -