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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK823
Library VH
(Link to library)
Clone ID VHK823
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16584-1
Original site URL
Representative seq. ID VHK823P
(Link to Original site)
Representative DNA sequence 
>VHK823 (VHK823Q) /CSM/VH/VHK8-A/VHK823Q.Seq.d/
ATTAAAAAAAATTCTAGTAAGGTATAAAAAAAAAATCATATAAAATGGTAGAAAAAATCA
TTGTTTCAAATCCAGTTGCTAATCTTTTAGGTGATGAACAAACCAGAGTTATTTGGGATT
TAATTGAAAAGAAATTAATTTTTCCATTTTTAGATTTAAAAGTTGAAACCTATGATTTAG
GAATTGAGTATCGTGATAAAACCAATGATCAAGTTACAATCGATGCAGCCAATGCAATTA
ANAGATTAAAAGTTGGTATTAAATGTGCAACAATTACACCAGACGAAGCACGTGTCACAG
AGTTTGGATTGAAGGANATGTGGAAATCACCAAATGGTACCATTCGTAATACATTGGGTG
GCACATTATTTANAGAGCCAATTGTTTGTAANAATGTCCCACGTTTGGTCACCTGTTGGA
ATAAATCTATAGTTATTGGTCGTCATGCATTTGGTGACCAATACAGAGCCACTGATTTCG
TAGTAAAGGGAGCTGGCAAGTTGGAGTTAACCTACACACCAGCCGACGGCAGTGCACCAC
AAAAATTCCAAGTCTTTGACTTCCXXXXXXXXXXGATACGACGGCCGTTTCAAAGACATT
TTCCAAGAGATCTACGAAAGAGAGTACAAAGTTAAATTCGACACTGCCGGTATTTGGTAT
GAGCATAGACTCATCGACGACATGGTCGCCTATGCTATGAAGAGTGAGGGTGGCTTCGTT
TGGGCTNGTAAAAACTATGACGGTGATGTTCAATCANATGCCGTCGCCCAGGGTTATGGT
AGTTTGGGTNTCATGACTTCAGTTTTACTCTCTGCCGACTCTTTAGNTGCTGAAGCCTCC
CATGGTACTGTCACTAGACATTTCCGTGANCATCNAAAAGGTAGAGAAACCAGTACCAAT
TCAATCGCTTNNATCTTTGC
sequence update 2002.10.25
Translated Amino Acid sequence 
*kkf**GIKKKSYKMVEKIIVSNPVANLLGDEQTRVIWDLIEKKLIFPFLDLKVETYDLG
IEYRDKTNDQVTIDAANAIXRLKVGIKCATITPDEARVTEFGLKXMWKSPNGTIRNTLGG
TLFXEPIVCXNVPRLVTCWNKSIVIGRHAFGDQYRATDFVVKGAGKLELTYTPADGSAPQ
KFQVFDF---

---YDGRFKDIFQEIYEREYKVKFDTAGIWYEHRLIDDMVAYAMKSEGGFVWAXKNYDGD
VQSXAVAQGYGSLGXMTSVLLSADSLXAEASHGTVTRHFRXHXKGRETSTNSIAXIF
Translated Amino Acid sequence (All Frames) 
Frame A:
ikknsskv*kknhikw*kkslfqiqllif*vmnkpelfgi*lkrn*ffhf*i*klkpmi*
elsivikpmiklqsmqpmqlxd*klvlnvqqlhqtkhvsqsld*rxcgnhqmvpfvihwv
ahylxsqlfvxmshvwspvginl*llvvmhlvtnteplis**relasws*pthqptavhh
knskslts---

---dttavsktfskrstkestklnstlpvfgmsidssttwspml*rvrvasfglvktmtv
mfnxmpsprvmvvwvs*lqfyslptl*xlkppmvlsldisvxixkvekpvpiqslxsl

Frame B:
lkkilvrykkkii*ngrknhcfkssc*sfr**tnqsylgfn*keinfsifrfks*nl*fr
n*vs**nq*ssynrcsqcn*xikswy*mcnnytrrstchrvwiegxveitkwyhs*yigw
hiixrancl*xcptfghlle*iysywssciw*piqsh*frskgswqvgvnlhtsrrqctt
kipsl*l---

---irrpfqrhfprdlrkrvqs*irhcrylv*a*thrrhgrlcyee*gwlrlgx*kl*r*
csixcrrpglw*fgxhdfsftlcrlfxc*slpwych*tfp*xskr*rnqyqfnrxxlc

Frame C:
*kkf**GIKKKSYKMVEKIIVSNPVANLLGDEQTRVIWDLIEKKLIFPFLDLKVETYDLG
IEYRDKTNDQVTIDAANAIXRLKVGIKCATITPDEARVTEFGLKXMWKSPNGTIRNTLGG
TLFXEPIVCXNVPRLVTCWNKSIVIGRHAFGDQYRATDFVVKGAGKLELTYTPADGSAPQ
KFQVFDF---

---YDGRFKDIFQEIYEREYKVKFDTAGIWYEHRLIDDMVAYAMKSEGGFVWAXKNYDGD
VQSXAVAQGYGSLGXMTSVLLSADSLXAEASHGTVTRHFRXHXKGRETSTNSIAXIF
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK823 (VHK823Q) /CSM/VH/VHK8-A/VHK823Q.Seq.d/                       1580   0.0
VFG531 (VFG531Q) /CSM/VF/VFG5-B/VFG531Q.Seq.d/                       1566   0.0
VSJ261 (VSJ261Q) /CSM/VS/VSJ2-C/VSJ261Q.Seq.d/                        973   0.0
VFM756 (VFM756Q) /CSM/VF/VFM7-C/VFM756Q.Seq.d/                        973   0.0
VFM475 (VFM475Q) /CSM/VF/VFM4-D/VFM475Q.Seq.d/                        973   0.0
VFM444 (VFM444Q) /CSM/VF/VFM4-B/VFM444Q.Seq.d/                        973   0.0
VFL572 (VFL572Q) /CSM/VF/VFL5-C/VFL572Q.Seq.d/                        973   0.0
VFL349 (VFL349Q) /CSM/VF/VFL3-C/VFL349Q.Seq.d/                        973   0.0
VFL180 (VFL180Q) /CSM/VF/VFL1-D/VFL180Q.Seq.d/                        973   0.0
VFF796 (VFF796Q) /CSM/VF/VFF7-D/VFF796Q.Seq.d/                        973   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116963) Dictyostelium discoideum chromosome 2 map 4657875...   880   0.0    3
(BJ424361) Dictyostelium discoideum cDNA clone:ddv52n06, 5' ...   981   0.0    1
(AU269659) Dictyostelium discoideum vegetative cDNA clone:VS...   973   0.0    1
(BJ416528) Dictyostelium discoideum cDNA clone:ddv26p13, 5' ...   973   0.0    1
(BJ416327) Dictyostelium discoideum cDNA clone:ddv25f20, 5' ...   973   0.0    1
(BJ416217) Dictyostelium discoideum cDNA clone:ddv25h12, 5' ...   973   0.0    1
(BJ416046) Dictyostelium discoideum cDNA clone:ddv24o17, 5' ...   973   0.0    1
(BJ415848) Dictyostelium discoideum cDNA clone:ddv23o19, 5' ...   973   0.0    1
(BJ415717) Dictyostelium discoideum cDNA clone:ddv23b13, 5' ...   973   0.0    1
(BJ413161) Dictyostelium discoideum cDNA clone:ddv14m07, 5' ...   973   0.0    1
dna update 2008.11.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q75JR3) RecName: Full=Isocitrate dehydrogenase [NADP] cytoplasm...   343   4e-93
CP000481_386(CP000481|pid:none) Acidothermus cellulolyticus 11B ...   239   7e-62
AM849034_593(AM849034|pid:none) Clavibacter michiganensis subsp....   239   1e-61
AM270034_12(AM270034|pid:none) Aspergillus niger contig An02c040...   237   5e-61
AP008957_2005(AP008957|pid:none) Rhodococcus erythropolis PR4 DN...   237   5e-61
AM920435_361(AM920435|pid:none) Penicillium chrysogenum Wisconsi...   237   5e-61
DQ886272_1(DQ886272|pid:none) Gryllus firmus isocitrate dehydrog...   236   6e-61
AP011115_6297(AP011115|pid:none) Rhodococcus opacus B4 DNA, comp...   236   6e-61
DQ440329_1(DQ440329|pid:none) Aedes aegypti clone AET-426 NADP-d...   234   3e-60
CP000084_639(CP000084|pid:none) Candidatus Pelagibacter ubique H...   234   4e-60
protein update 2009. 7.15
PSORT 

 psg: 0.74  gvh: 0.35  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHK823 is cyt
5' end seq. ID VHK823F
5' end seq. 
>VHK823F.Seq
ATTAAAAAAAATTCTAGTAAGGTATAAAAAAAAAATCATATAAAATGGTAGAAAAAATCA
TTGTTTCAAATCCAGTTGCTAATCTTTTAGGTGATGAACAAACCAGAGTTATTTGGGATT
TAATTGAAAAGAAATTAATTTTTCCATTTTTAGATTTAAAAGTTGAAACCTATGATTTAG
GAATTGAGTATCGTGATAAAACCAATGATCAAGTTACAATCGATGCAGCCAATGCAATTA
ANAGATTAAAAGTTGGTATTAAATGTGCAACAATTACACCAGACGAAGCACGTGTCACAG
AGTTTGGATTGAAGGANATGTGGAAATCACCAAATGGTACCATTCGTAATACATTGGGTG
GCACATTATTTANAGAGCCAATTGTTTGTAANAATGTCCCACGTTTGGTCACCTGTTGGA
ATAAATCTATAGTTATTGGTCGTCATGCATTTGGTGACCAATACAGAGCCACTGATTTCG
TAGTAAAGGGAGCTGGCAAGTTGGAGTTAACCTACACACCAGCCGACGGCAGTGCACCAC
AAAAATTCCAAGTCTTTGACTTCCNNNNNNNNNN
Length of 5' end seq. 574
3' end seq. ID VHK823Z
3' end seq. 
>VHK823Z.Seq
NNNNNNNNNNGATACGACGGCCGTTTCAAAGACATTTTCCAAGAGATCTACGAAAGAGAG
TACAAAGTTAAATTCGACACTGCCGGTATTTGGTATGAGCATAGACTCATCGACGACATG
GTCGCCTATGCTATGAAGAGTGAGGGTGGCTTCGTTTGGGCTNGTAAAAACTATGACGGT
GATGTTCAATCANATGCCGTCGCCCAGGGTTATGGTAGTTTGGGTNTCATGACTTCAGTT
TTACTCTCTGCCGACTCTTTAGNTGCTGAAGCCTCCCATGGTACTGTCACTAGACATTTC
CGTGANCATCNAAAAGGTAGAGAAACCAGTACCAATTCAATCGCTTNNATCTTTGC
Length of 3' end seq. 356
Connected seq. ID VHK823P
Connected seq. 
>VHK823P.Seq
ATTAAAAAAAATTCTAGTAAGGTATAAAAAAAAAATCATATAAAATGGTAGAAAAAATCA
TTGTTTCAAATCCAGTTGCTAATCTTTTAGGTGATGAACAAACCAGAGTTATTTGGGATT
TAATTGAAAAGAAATTAATTTTTCCATTTTTAGATTTAAAAGTTGAAACCTATGATTTAG
GAATTGAGTATCGTGATAAAACCAATGATCAAGTTACAATCGATGCAGCCAATGCAATTA
ANAGATTAAAAGTTGGTATTAAATGTGCAACAATTACACCAGACGAAGCACGTGTCACAG
AGTTTGGATTGAAGGANATGTGGAAATCACCAAATGGTACCATTCGTAATACATTGGGTG
GCACATTATTTANAGAGCCAATTGTTTGTAANAATGTCCCACGTTTGGTCACCTGTTGGA
ATAAATCTATAGTTATTGGTCGTCATGCATTTGGTGACCAATACAGAGCCACTGATTTCG
TAGTAAAGGGAGCTGGCAAGTTGGAGTTAACCTACACACCAGCCGACGGCAGTGCACCAC
AAAAATTCCAAGTCTTTGACTTCC----------GATACGACGGCCGTTTCAAAGACATT
TTCCAAGAGATCTACGAAAGAGAGTACAAAGTTAAATTCGACACTGCCGGTATTTGGTAT
GAGCATAGACTCATCGACGACATGGTCGCCTATGCTATGAAGAGTGAGGGTGGCTTCGTT
TGGGCTNGTAAAAACTATGACGGTGATGTTCAATCANATGCCGTCGCCCAGGGTTATGGT
AGTTTGGGTNTCATGACTTCAGTTTTACTCTCTGCCGACTCTTTAGNTGCTGAAGCCTCC
CATGGTACTGTCACTAGACATTTCCGTGANCATCNAAAAGGTAGAGAAACCAGTACCAAT
TCAATCGCTTNNATCTTTGC
Length of connected seq. 910
Full length Seq ID -
Full length Seq. -
Length of full length seq. -