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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK810
Library VH
(Link to library)
Clone ID VHK810
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11249-1|Contig-U12842-1
Original site URL
Representative seq. ID VHK810P
(Link to Original site)
Representative DNA sequence 
>VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/
ATTTTTAAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTT
TAAGAGATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATG
CAGAAGATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATA
ATGGGTTGTTAATTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTC
CATCAATTTTAATTTATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTC
AAATTAGTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTT
GGAACAGTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGT
TCTCAGATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATG
ATTATAAGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATCTAAAGAATCAAATTGTA
ACCCAAAAGAATATTTTAATGGTACAATCATTCGTCTACCAXXXXXXXXXXCTTTACACG
AGTGATCAAGTCTATGATCTCTTATTCAAATATTATGGTAATAAGTTGGCCAAACATTTA
AAATTTTCTACATTACCATTAAACTTTAGACAATTAATTTCATCAATTTGTAGAAATTAT
ATAAATATTAGAGAAATTAAACTTTCAGATTTTATCACTAGTCTCAACTCCATCAACAAC
CTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAATTGTATCGATGAATTAAAAAGA
AAAGAAACTTTAGAATTATCTCATATCCAATCATTACAAATTATCCCATATAGATTAAGT
CCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTCATTGTTAATGTACAAATCAAAT
TTCTTACCAATTGAACCAATCTTAAACAAACTAAAAATCTATTTAATAAAAGAAGGACAT
CAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCCAATTTTATTAGGCTATCTTCAA
AGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAAATTAAGAATATTTTTAGTTGAA
TCAATGTTCCAAATTTCTTTATCACAAGATTATACTTCAATTTTTTATAATTTACCAATT
CACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAGTATTACAACCAGTAGTTACTGT
GTGGATTATGAAAAAATTGATCAATATGAAATTCCAGGATTAAAATTAATAAAGGTTAAN
AATTAACAAAATATAGAAATCTTTCGATACAATACC
sequence update 2002.10.25
Translated Amino Acid sequence 
f*RDKMPQNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNN
GLLISNDNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGW
NSVYHITDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQSKESNCN
PKEYFNGTIIRLP---

---LYTSDQVYDLLFKYYGNKLAKHLKFSTLPLNFRQLISSICRNYINIREIKLSDFITS
LNSINNLQFLSDSIITIWNCIDELKRKETLELSHIQSLQIIPYRLSPTETDVTSFDKSSL
LMYKSNFLPIEPILNKLKIYLIKEGHQINSLLDGNAQIPILLGYLQRVNLSLDSKEIDKL
RIFLVESMFQISLSQDYTSIFYNLPIHPCIGNSNQKFSSITTSSYCVDYEKIDQYEIPGL
KLIKVXN*qnieifrynt
Translated Amino Acid sequence (All Frames) 
Frame A:
ifkeikcpkikvkq*nylif*eiifltiqnqkylen*fkmqkmleliqs**rlmkahiqi
mgc*fqmitkklkvqkny*vhqf*fiimqlfknmigkefvklvkvvkkki*kvlaisv*v
gtvfii*qiiqlfivvimfgsqiqtke*vvvysli*mtkmiirnvlvf*shsfnlknqiv
tqknilmvqsfvy---

---LYTSDQVYDLLFKYYGNKLAKHLKFSTLPLNFRQLISSICRNYINIREIKLSDFITS
LNSINNLQFLSDSIITIWNCIDELKRKETLELSHIQSLQIIPYRLSPTETDVTSFDKSSL
LMYKSNFLPIEPILNKLKIYLIKEGHQINSLLDGNAQIPILLGYLQRVNLSLDSKEIDKL
RIFLVESMFQISLSQDYTSIFYNLPIHPCIGNSNQKFSSITTSSYCVDYEKIDQYEIPGL
KLIKVXN*qnieifrynt

Frame B:
flkr*napk*ksnnktisffkr*ss*lsrikni*ridskcrrcss*yshnkg**rlisk*
wvvnfk**prn*kfkriirsinfnl**cnfsrt*lgrnlsn*sr**krkfkkcwqfrfrl
eqclsynr*sncl*w**clvlrskrknewwfil*fr*rr*l*emf*ffraihsi*rikl*
pkrif*wynhsst---

---ftrviksmisysnimviswpni*nflhyh*tldn*fhqfveii*ileklnfqilslv
stpsttsnf*viv**qfgivsmn*kekkl*nylisnhyklshid*vqlklmslvlinlhc
*ctnqisyqlnqs*tn*ksi**kkdiksivy*memlksqfy*aifkg*iyh*tqrksin*
eyf*lnqcskflyhkiilqffiiyqfthaleivtkslvvlqpvvtvwimkklinmkfqd*
n**rlxinki*ksfdti

Frame C:
f*RDKMPQNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNN
GLLISNDNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGW
NSVYHITDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQSKESNCN
PKEYFNGTIIRLP---

---lhe*ssl*sliqilw**vgqtfkifyitikl*tinfinl*klyky*rn*tfrfyh*s
qlhqqppifk**ynnnlelyr*ikkkrnfriisypiitnypi*iksn*n*ch*f**ifiv
nvqikfltn*tnlkqtknlfnkrrtsnq*firwkcsnpnfirlsskgkfiirlkgnr*ik
nifs*invpnffitrlyfnfl*ftnspmhwk**pkv**yynq*llcgl*kn*si*nsrik
inkg*xltkyrnlsiqy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/                       1957   0.0
VHD239 (VHD239Q) /CSM/VH/VHD2-B/VHD239Q.Seq.d/                       1409   0.0
VHE123 (VHE123Q) /CSM/VH/VHE1-A/VHE123Q.Seq.d/                       1394   0.0
VHO809 (VHO809Q) /CSM/VH/VHO8-A/VHO809Q.Seq.d/                       1356   0.0
VHB767 (VHB767Q) /CSM/VH/VHB7-C/VHB767Q.Seq.d/                       1356   0.0
VHK791 (VHK791Q) /CSM/VH/VHK7-D/VHK791Q.Seq.d/                       1298   0.0
VFN211 (VFN211Q) /CSM/VF/VFN2-A/VFN211Q.Seq.d/                       1176   0.0
VHN647 (VHN647Q) /CSM/VH/VHN6-B/VHN647Q.Seq.d/                        628   e-179
VHC102 (VHC102Q) /CSM/VH/VHC1-A/VHC102Q.Seq.d/                        628   e-179
VHE271 (VHE271Q) /CSM/VH/VHE2-C/VHE271Q.Seq.d/                        601   e-170
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ443141) Dictyostelium discoideum cDNA clone:ddv52d04, 3' ...  1511   0.0    1
(BJ439015) Dictyostelium discoideum cDNA clone:ddv39m05, 3' ...  1342   0.0    3
(BJ438600) Dictyostelium discoideum cDNA clone:ddv37m10, 3' ...  1342   0.0    3
(BJ445598) Dictyostelium discoideum cDNA clone:ddv60b04, 3' ...  1328   0.0    2
(BJ437533) Dictyostelium discoideum cDNA clone:ddv34f17, 3' ...  1328   0.0    2
(BJ443404) Dictyostelium discoideum cDNA clone:ddv52f23, 3' ...  1275   0.0    2
(BJ416709) Dictyostelium discoideum cDNA clone:ddv27e04, 5' ...  1176   0.0    1
(BJ426269) Dictyostelium discoideum cDNA clone:ddv58m12, 5' ...   628   0.0    2
(BJ424311) Dictyostelium discoideum cDNA clone:ddv52d04, 5' ...   628   0.0    2
(BJ418948) Dictyostelium discoideum cDNA clone:ddv34f17, 5' ...   628   0.0    2
dna update 2008.11.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9NZJ4) RecName: Full=Sacsin;  &AL157766_4(AL157766|pid:none)        105   5e-21
BC138482_1(BC138482|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   2e-20
(Q9JLC8) RecName: Full=Sacsin;                                        103   2e-20
BC171956_1(BC171956|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   2e-20
CP001577_292(CP001577|pid:none) Micromonas sp. RCC299 chromosome...   102   4e-20
BC142916_1(BC142916|pid:none) Danio rerio hypothetical LOC555303...   100   2e-19
AB006708_10(AB006708|pid:none) Arabidopsis thaliana genomic DNA,...    96   4e-18
AL732351_6(AL732351|pid:none) Oryza sativa genomic DNA, chromoso...    86   2e-15
AL606457_18(AL606457|pid:none) Oryza sativa genomic DNA, chromos...    85   7e-15
CR749427_1(CR749427|pid:none) Homo sapiens mRNA; cDNA DKFZp686B1...    76   3e-12
protein update 2009. 7.15
PSORT 

 psg: 0.68  gvh: 0.39  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHK810 is cyt
5' end seq. ID VHK810F
5' end seq. 
>VHK810F.Seq
ATTTTTAAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTT
TAAGAGATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATG
CAGAAGATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATA
ATGGGTTGTTAATTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTC
CATCAATTTTAATTTATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTC
AAATTAGTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTT
GGAACAGTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGT
TCTCAGATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATG
ATTATAAGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATCTAAAGAATCAAATTGTA
ACCCAAAAGAATATTTTAATGGTACAATCATTCGTCTACCANNNNNNNNNN
Length of 5' end seq. 591
3' end seq. ID VHK810Z
3' end seq. 
>VHK810Z.Seq
NNNNNNNNNNCTTTACACGAGTGATCAAGTCTATGATCTCTTATTCAAATATTATGGTAA
TAAGTTGGCCAAACATTTAAAATTTTCTACATTACCATTAAACTTTAGACAATTAATTTC
ATCAATTTGTAGAAATTATATAAATATTAGAGAAATTAAACTTTCAGATTTTATCACTAG
TCTCAACTCCATCAACAACCTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAATTG
TATCGATGAATTAAAAAGAAAAGAAACTTTAGAATTATCTCATATCCAATCATTACAAAT
TATCCCATATAGATTAAGTCCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTCATT
GTTAATGTACAAATCAAATTTCTTACCAATTGAACCAATCTTAAACAAACTAAAAATCTA
TTTAATAAAAGAAGGACATCAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCCAAT
TTTATTAGGCTATCTTCAAAGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAAATT
AAGAATATTTTTAGTTGAATCAATGTTCCAAATTTCTTTATCACAAGATTATACTTCAAT
TTTTTATAATTTACCAATTCACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAGTAT
TACAACCAGTAGTTACTGTGTGGATTATGAAAAAATTGATCAATATGAAATTCCAGGATT
AAAATTAATAAAGGTTAANAATTAACAAAATATAGAAATCTTTCGATACAATACC
Length of 3' end seq. 775
Connected seq. ID VHK810P
Connected seq. 
>VHK810P.Seq
ATTTTTAAAGAGATAAAATGCCCCAAAATAAAAGTCAAACAATAAAACTATCTCATTTTT
TAAGAGATAATCTTCTTAACTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATG
CAGAAGATGCTCGAGCTGATACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATA
ATGGGTTGTTAATTTCAAATGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTC
CATCAATTTTAATTTATAATAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTC
AAATTAGTCAAGGTAGTAAAAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTT
GGAACAGTGTTTATCATATAACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGT
TCTCAGATCCAAACGAAAGAATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATG
ATTATAAGAAATGTTTTAGTTTTTTAGAGCCATTCATTCAATCTAAAGAATCAAATTGTA
ACCCAAAAGAATATTTTAATGGTACAATCATTCGTCTACCA----------CTTTACACG
AGTGATCAAGTCTATGATCTCTTATTCAAATATTATGGTAATAAGTTGGCCAAACATTTA
AAATTTTCTACATTACCATTAAACTTTAGACAATTAATTTCATCAATTTGTAGAAATTAT
ATAAATATTAGAGAAATTAAACTTTCAGATTTTATCACTAGTCTCAACTCCATCAACAAC
CTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAATTGTATCGATGAATTAAAAAGA
AAAGAAACTTTAGAATTATCTCATATCCAATCATTACAAATTATCCCATATAGATTAAGT
CCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTCATTGTTAATGTACAAATCAAAT
TTCTTACCAATTGAACCAATCTTAAACAAACTAAAAATCTATTTAATAAAAGAAGGACAT
CAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCCAATTTTATTAGGCTATCTTCAA
AGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAAATTAAGAATATTTTTAGTTGAA
TCAATGTTCCAAATTTCTTTATCACAAGATTATACTTCAATTTTTTATAATTTACCAATT
CACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAGTATTACAACCAGTAGTTACTGT
GTGGATTATGAAAAAATTGATCAATATGAAATTCCAGGATTAAAATTAATAAAGGTTAAN
AATTAACAAAATATAGAAATCTTTCGATACAATACC
Length of connected seq. 1346
Full length Seq ID -
Full length Seq. -
Length of full length seq. -