>AFO449 (AFO449Q) /CSM/AF/AFO4-C/AFO449Q.Seq.d/
AGTGTCATAATTCATCAAGCAAAACAACCACAACAAGAACAACAACAACAAATGTCAATT
TTAAAAAGTTTTATTGAAGTTAGTGAAGATTCACACTTTCCAATTCAAAATCTTCCATAT
GGTGTCTTTAAACCAACCCTAAATGATCAAGCTAGAATTGGTGTTGCTATTGGTGATTTC
GTTTGTGATTTATCAGTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATACT
AAAGTTTTTCATCAAGAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGAGT
GAAGCTAGAAAAACCATTCAAAATTTATTATCATCAGAAACATCAACTATTAGAGATAAT
AAAGAGTATAGAGAAAAGATATTTCATTCAATTTCAAGTGTAACAATGTTATTACCAGCA
AGAATTGGAGATTATACTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTATT
ATGTTTAGAGGTAAAGAGAATGCATTGATGCCAAATTGGGTACATTTACCAGTTGGTTAT
CATGGTAGATCATCATCAATAGTCGTAAGTGGTACACCATTGAAAAGACCXXXXXXXXXX
AACCAGATTCATTACCAACTTTTAATCCATGTAGATTATTGGATTTTGAATTGGAGATGG
GCGCTTTGATTGGAGGAGAATCAACCAAATTGGGTGAACCAATTTCAATTGAGTCTGCAA
AAGATCATATCTTTGGTTTGGTACTTTTGAATGATTGGAGTGCAAGGGATATCCAAAAAT
GGGAATACGTTCCATTGGGTCCATTCTTGGCAAAGAATTTCGGCTCAACTATATCACCAT
GGGTTGTTACAATGGAGGCATTGCAACCATTTGCTACAAAACCACCAACTCAAGATCCAC
AACCAATGAAATATCTTCAAGAACAAGGTAATACTACCTTTGATATTGAATTATCAGTTA
GTATTAAATCTCCAAAAATGTCAAAACCTCATAAGGTTTCAACTTCAAATTTGAAATACA
TGTATTGGACACTCACTCAACAATTGGCTCATCATACTGTAAATGGTTGTAATATGAATG
CTGGTGATTTACTTGGTACTGGTACAATTAGTGGTCCAACTGAAGATAGCTATGGTTCAA
TGTTGGAACTCTCTTGGAAAGGTAGTAAAGTGGTCTCATTAGGTACTGAAACAAATGAAG
AAAGAAAATTCATTCAAGATGGTGATTCTGTTATCTTATCTGGTCTTTGTAAAGGTAAGG
TTATCAAAT

SVIIHQAKQPQQEQQQQMSILKSFIEVSEDSHFPIQNLPYGVFKPTLNDQARIGVAIGDF
VCDLSVLADLKLFDGKLKDTKVFHQENLNSFMSLGKELWSEARKTIQNLLSSETSTIRDN
KEYREKIFHSISSVTMLLPARIGDYTDFYASKEHATNVGIMFRGKENALMPNWVHLPVGY
HGRSSSIVVSGTPLKR---

---PDSLPTFNPCRLLDFELEMGALIGGESTKLGEPISIESAKDHIFGLVLLNDWSARDI
QKWEYVPLGPFLAKNFGSTISPWVVTMEALQPFATKPPTQDPQPMKYLQEQGNTTFDIEL
SVSIKSPKMSKPHKVSTSNLKYMYWTLTQQLAHHTVNGCNMNAGDLLGTGTISGPTEDSY
GSMLELSWKGSKVVSLGTETNEERKFIQDGDSVILSGLCKGKVIK

Frame A:
SVIIHQAKQPQQEQQQQMSILKSFIEVSEDSHFPIQNLPYGVFKPTLNDQARIGVAIGDF
VCDLSVLADLKLFDGKLKDTKVFHQENLNSFMSLGKELWSEARKTIQNLLSSETSTIRDN
KEYREKIFHSISSVTMLLPARIGDYTDFYASKEHATNVGIMFRGKENALMPNWVHLPVGY
HGRSSSIVVSGTPLKR---

---nqihyqllihvdywilnwrwal*leenqpnwvnqfqlslqkiislvwyf*migvqgi
skngntfhwvhswqrisaqlyhhgllqwrhcnhllqnhqlkihnq*nifknkvilpliln
yqlvlnlqkcqnlirfqlqi*ntcighslnnwliil*mvvi*mlviylvlvqlvvqlkia
mvqcwnslgkvvkwsh*vlkqmkkensfkmvillsylvfvkvrlsn

Frame B:
vs*fikqnnhnknnnnkcqf*kvllklvkihtfqfkifhmvslnqp*miklelvlllvis
fviyqf*qi*nflmvn*kilkffikki*ihlch*vknygvklekpfkiyyhqkhqlleii
ksiekryfiqfqv*qcyyqqeleiilismhqknmqpmlvlclevkrmh*cqigyiyqlvi
mvdhhq*s*vvhh*kd---

---trfitnf*sm*iigf*igdgrfdwrrinqig*tnfn*vckrsylwfgtfe*leckgy
pkmgirsigsilgkefrlnyitmgcynggiaticykttnsrsttneissrtr*yyl*y*i
is*y*isknvkts*gfnfkfeihvldthstigssyckwl*yecw*ftwywyn*wsn*r*l
wfnvgtller**sgliry*nk*rkkihsrw*fcyliwsl*r*gyq

Frame C:
chnssskttttrttttnvnfkkfy*s**rftlsnskssiwcl*tnpk*ss*nwccyw*fr
l*fisfsrfktf*w*ikry*sfssrkfkfiyvir*rime*s*knhskfiiirniny*r**
rv*rkdisfnfkcnnvitsknwrly*flcikrtcnqcwyyv*r*recidaklgtftswls
w*iiinsrkwytiekt---

---PDSLPTFNPCRLLDFELEMGALIGGESTKLGEPISIESAKDHIFGLVLLNDWSARDI
QKWEYVPLGPFLAKNFGSTISPWVVTMEALQPFATKPPTQDPQPMKYLQEQGNTTFDIEL
SVSIKSPKMSKPHKVSTSNLKYMYWTLTQQLAHHTVNGCNMNAGDLLGTGTISGPTEDSY
GSMLELSWKGSKVVSLGTETNEERKFIQDGDSVILSGLCKGKVIK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO449 (AFO449Q) /CSM/AF/AFO4-C/AFO449Q.Seq.d/                       2355   0.0
CFA612 (CFA612Q) /CSM/CF/CFA6-A/CFA612Q.Seq.d/                       1318   0.0
AFK792 (AFK792Q) /CSM/AF/AFK7-D/AFK792Q.Seq.d/                       1318   0.0
SFD819 (SFD819Q) /CSM/SF/SFD8-A/SFD819Q.Seq.d/                       1304   0.0
AFJ754 (AFJ754Q) /CSM/AF/AFJ7-C/AFJ754Q.Seq.d/                       1296   0.0
AFO620 (AFO620Q) /CSM/AF/AFO6-A/AFO620Q.Seq.d/                       1281   0.0
AFN775 (AFN775Q) /CSM/AF/AFN7-D/AFN775Q.Seq.d/                       1255   0.0
AFE706 (AFE706Q) /CSM/AF/AFE7-A/AFE706Q.Seq.d/                       1253   0.0
CHD537 (CHD537Q) /CSM/CH/CHD5-B/CHD537Q.Seq.d/                       1237   0.0
VFM365 (VFM365Q) /CSM/VF/VFM3-C/VFM365Q.Seq.d/                       1207   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BM774584|BM774584.1 ra95c11.y1 Meloidogyne incognita egg pAMP1 Bird Meloidogyne incognita cDNA 5' similar to TR:Q94272 Q94272 SIMILAR TO FUMARYLACETOACETASE. [1] ;, mRNA sequence.    56  5e-18  3
BI504681|BI504681.1 BB170011A20F01 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170011A20F01 5', mRNA sequence.    56  9e-14  3
BG226093|BG226093.1 kq09b06.y1 TBN95TM-SSR Strongyloides stercoralis cDNA 5' similar to SW:FAAA_HUMAN P16930 FUMARYLACETOACETASE ;, mRNA sequence.    34  3e-08  5
BQ475268|BQ475268.1 cicindela4h01.b Cicindela campestris cDNA Cicindela campestris cDNA clone cicindela4h01 5', mRNA sequence.    56  5e-08  2
CB377955|CB377955.1 rq05b07.y1 Heterodera glycines J3 Heterodera glycines cDNA 5' similar to TR:Q94272 Q94272 SIMILAR TO FUMARYLACETOACETASE. [1] ;, mRNA sequence.    70  7e-08  1
AV384197|AV384197.1 Halocynthia roretzi cDNA clone:008K24_5, 5' end, expressed in fertilized egg.    42  3e-05  3
AW827814|AW827814.1 ra59b10.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to WP:K10C2.4 CE04750 FUMARYLACETOACETASE ;, mRNA sequence.    40  8e-05  3
AW871572|AW871572.1 ra79e06.y1 Bird-Rao Meloidogyne incognita J2 Meloidogyne incognita cDNA 5' similar to WP:K10C2.4 CE04750 FUMARYLACETOACETASE ;, mRNA sequence.    40  9e-05  3
CD419451|CD419451.1 rj11a04.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodi cDNA 5' similar to SW:FAAA_RAT P25093 FUMARYLACETOACETASE ;, mRNA sequence.    42  3e-04  3
CF440839|CF440839.1 EST677184 normalized cDNA library of onion Allium cepa cDNA clone ACAFM72, mRNA sequence.    56  0.001  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q1ZXQ1) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   451   0.0
(A5PKH3) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   275   e-131
AK143759_1(AK143759|pid:none) Mus musculus 6 days neonate spleen...   263   e-130
AY891819_1(AY891819|pid:none) Synthetic construct Homo sapiens c...   273   e-129
(P16930) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   273   e-129
A56825(A56825;S21387) fumarylacetoacetase (EC 3.7.1.2) - mouse  ...   263   e-129
EF144286_1(EF144286|pid:none) Populus trichocarpa clone PX0015_F...   268   e-127
(P25093) RecName: Full=Fumarylacetoacetase;          Short=FAA; ...   255   e-127
DQ311173_1(DQ311173|pid:none) Bombyx mori fumarylacetoacetate hy...   270   e-127
BC157696_1(BC157696|pid:none) Xenopus tropicalis hypothetical pr...   266   e-125

 psg: 0.41  gvh: 0.26  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for AFO449 is cyt

>AFO449F.Seq
AGTGTCATAATTCATCAAGCAAAACAACCACAACAAGAACAACAACAACAAATGTCAATT
TTAAAAAGTTTTATTGAAGTTAGTGAAGATTCACACTTTCCAATTCAAAATCTTCCATAT
GGTGTCTTTAAACCAACCCTAAATGATCAAGCTAGAATTGGTGTTGCTATTGGTGATTTC
GTTTGTGATTTATCAGTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATACT
AAAGTTTTTCATCAAGAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGAGT
GAAGCTAGAAAAACCATTCAAAATTTATTATCATCAGAAACATCAACTATTAGAGATAAT
AAAGAGTATAGAGAAAAGATATTTCATTCAATTTCAAGTGTAACAATGTTATTACCAGCA
AGAATTGGAGATTATACTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTATT
ATGTTTAGAGGTAAAGAGAATGCATTGATGCCAAATTGGGTACATTTACCAGTTGGTTAT
CATGGTAGATCATCATCAATAGTCGTAAGTGGTACACCATTGAAAAGACC----------

>AFO449Z.Seq
----------AACCAGATTCATTACCAACTTTTAATCCATGTAGATTATTGGATTTTGAA
TTGGAGATGGGCGCTTTGATTGGAGGAGAATCAACCAAATTGGGTGAACCAATTTCAATT
GAGTCTGCAAAAGATCATATCTTTGGTTTGGTACTTTTGAATGATTGGAGTGCAAGGGAT
ATCCAAAAATGGGAATACGTTCCATTGGGTCCATTCTTGGCAAAGAATTTCGGCTCAACT
ATATCACCATGGGTTGTTACAATGGAGGCATTGCAACCATTTGCTACAAAACCACCAACT
CAAGATCCACAACCAATGAAATATCTTCAAGAACAAGGTAATACTACCTTTGATATTGAA
TTATCAGTTAGTATTAAATCTCCAAAAATGTCAAAACCTCATAAGGTTTCAACTTCAAAT
TTGAAATACATGTATTGGACACTCACTCAACAATTGGCTCATCATACTGTAAATGGTTGT
AATATGAATGCTGGTGATTTACTTGGTACTGGTACAATTAGTGGTCCAACTGAAGATAGC
TATGGTTCAATGTTGGAACTCTCTTGGAAAGGTAGTAAAGTGGTCTCATTAGGTACTGAA
ACAAATGAAGAAAGAAAATTCATTCAAGATGGTGATTCTGTTATCTTATCTGGTCTTTGT
AAAGGTAAGGTTATCAAAT

>AFO449P.Seq
AGTGTCATAATTCATCAAGCAAAACAACCACAACAAGAACAACAACAACAAATGTCAATT
TTAAAAAGTTTTATTGAAGTTAGTGAAGATTCACACTTTCCAATTCAAAATCTTCCATAT
GGTGTCTTTAAACCAACCCTAAATGATCAAGCTAGAATTGGTGTTGCTATTGGTGATTTC
GTTTGTGATTTATCAGTTTTAGCAGATTTAAAACTTTTTGATGGTAAATTAAAAGATACT
AAAGTTTTTCATCAAGAAAATTTAAATTCATTTATGTCATTAGGTAAAGAATTATGGAGT
GAAGCTAGAAAAACCATTCAAAATTTATTATCATCAGAAACATCAACTATTAGAGATAAT
AAAGAGTATAGAGAAAAGATATTTCATTCAATTTCAAGTGTAACAATGTTATTACCAGCA
AGAATTGGAGATTATACTGATTTCTATGCATCAAAAGAACATGCAACCAATGTTGGTATT
ATGTTTAGAGGTAAAGAGAATGCATTGATGCCAAATTGGGTACATTTACCAGTTGGTTAT
CATGGTAGATCATCATCAATAGTCGTAAGTGGTACACCATTGAAAAGACC----------
AACCAGATTCATTACCAACTTTTAATCCATGTAGATTATTGGATTTTGAATTGGAGATGG
GCGCTTTGATTGGAGGAGAATCAACCAAATTGGGTGAACCAATTTCAATTGAGTCTGCAA
AAGATCATATCTTTGGTTTGGTACTTTTGAATGATTGGAGTGCAAGGGATATCCAAAAAT
GGGAATACGTTCCATTGGGTCCATTCTTGGCAAAGAATTTCGGCTCAACTATATCACCAT
GGGTTGTTACAATGGAGGCATTGCAACCATTTGCTACAAAACCACCAACTCAAGATCCAC
AACCAATGAAATATCTTCAAGAACAAGGTAATACTACCTTTGATATTGAATTATCAGTTA
GTATTAAATCTCCAAAAATGTCAAAACCTCATAAGGTTTCAACTTCAAATTTGAAATACA
TGTATTGGACACTCACTCAACAATTGGCTCATCATACTGTAAATGGTTGTAATATGAATG
CTGGTGATTTACTTGGTACTGGTACAATTAGTGGTCCAACTGAAGATAGCTATGGTTCAA
TGTTGGAACTCTCTTGGAAAGGTAGTAAAGTGGTCTCATTAGGTACTGAAACAAATGAAG
AAAGAAAATTCATTCAAGATGGTGATTCTGTTATCTTATCTGGTCTTTGTAAAGGTAAGG
TTATCAAAT