NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK791
Library VH
(Link to library)
Clone ID VHK791
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11249-1|Contig-U12842-1
Original site URL
Representative seq. ID VHK791P
(Link to Original site)
Representative DNA sequence 
>VHK791 (VHK791Q) /CSM/VH/VHK7-D/VHK791Q.Seq.d/
GATTAAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAGATAATCTTCTTAA
CTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAGATGCTCGAGCTGA
TACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGTTGTTAATTTCAAA
TGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAATTTTAATTTATAA
TAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTAGTCAAGGTAGTAA
AAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACAGTGTTTATCATAT
AACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAGATCCAAACGAAAG
AATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATAAGAAATGTTTTAG
TTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAAAAGAATATTTTAA
TGGTACAATCATTCGTCTACCATTGAGAATXXXXXXXXXXCTCTTATTCAAATATTATGG
TAATAAGTTGGCCAAACATTTAAAATTTTCTACATTACCATTAAACTTTAGACAATTAAT
TTCATCAATTTGTAGAAATTATATAAATATTAGAGAAATTAAACTTTCAGATTTTATCAC
TAGTCTCAACTCCATCAACAACCTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAA
TTGTATCGATGAATTAAAAAGAAAAGAAACTTTAGAATTATCTCATATCCAATCATTACA
AATTATCCCATATAGATTAAGTCCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTC
ATTGTTAATGTACAAATCAAATTTCTTACCAATTGAACCAATCTTAAACAAACTAAAAAT
CTATTTAATAAAAGAAGGACATCAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCC
AATTTTATTAGGCTATCTTCAAAGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAA
ATTAAGAATATTTTTAGTTGAATCAATGTTCCAAATTTCTTTATCACAAGATTATACTTC
AATTTTTTATAATTTACCAATTCACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAG
TATTACAACCAGTAGTTACTGTGGGATTAGAAAAAATGATCAATANAAATTCCAGGNATT
AAAATTAATAAAGGG
sequence update 2002.10.25
Translated Amino Acid sequence 
IKNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNNGLLISN
DNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGWNSVYHI
TDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQFKESNCNPKEYFN
GTIIRLPLR---

---LLFKYYGNKLAKHLKFSTLPLNFRQLISSICRNYINIREIKLSDFITSLNSINNLQF
LSDSIITIWNCIDELKRKETLELSHIQSLQIIPYRLSPTETDVTSFDKSSLLMYKSNFLP
IEPILNKLKIYLIKEGHQINSLLDGNAQIPILLGYLQRVNLSLDSKEIDKLRIFLVESMF
QISLSQDYTSIFYNLPIHPCIGNSNQKFSSITTSSYCGIRKNDQXKFQXLKLIK
Translated Amino Acid sequence (All Frames) 
Frame A:
d*k*ksnnktisffkr*ss*lsrikni*ridskcrrcss*yshnkg**rlisk*wvvnfk
**prn*kfkriirsinfnl**cnfsrt*lgrnlsn*sr**krkfkkcwqfrfrleqclsy
nr*sncl*w**clvlrskrknewwfil*fr*rr*l*emf*ffraihsi*rikl*pkrif*
wynhsstien---

---LLFKYYGNKLAKHLKFSTLPLNFRQLISSICRNYINIREIKLSDFITSLNSINNLQF
LSDSIITIWNCIDELKRKETLELSHIQSLQIIPYRLSPTETDVTSFDKSSLLMYKSNFLP
IEPILNKLKIYLIKEGHQINSLLDGNAQIPILLGYLQRVNLSLDSKEIDKLRIFLVESMF
QISLSQDYTSIFYNLPIHPCIGNSNQKFSSITTSSYCGIRKNDQXKFQXLKLIK

Frame B:
IKNKSQTIKLSHFLRDNLLNYPESKIFRELIQNAEDARADTVIIKVDEGSYPNNGLLISN
DNQEIESSKELLGPSILIYNNATFQEHDWEGICQISQGSKKENLKSVGNFGLGWNSVYHI
TDNPIVYSGNNVWFSDPNERMSGGLFFDLDDEDDYKKCFSFLEPFIQFKESNCNPKEYFN
GTIIRLPLR---

---sysnimviswpni*nflhyh*tldn*fhqfveii*ileklnfqilslvstpsttsnf
*viv**qfgivsmn*kekkl*nylisnhyklshid*vqlklmslvlinlhc*ctnqisyq
lnqs*tn*ksi**kkdiksivy*memlksqfy*aifkg*iyh*tqrksin*eyf*lnqcs
kflyhkiilqffiiyqfthaleivtkslvvlqpvvtvglekminxnsrx*n**r

Frame C:
lkikvkq*nylif*eiifltiqnqkylen*fkmqkmleliqs**rlmkahiqimgc*fqm
itkklkvqkny*vhqf*fiimqlfknmigkefvklvkvvkkki*kvlaisv*vgtvfii*
qiiqlfivvimfgsqiqtke*vvvysli*mtkmiirnvlvf*shsfnlknqivtqknilm
vqsfvyh*e---

---liqilw**vgqtfkifyitikl*tinfinl*klyky*rn*tfrfyh*sqlhqqppif
k**ynnnlelyr*ikkkrnfriisypiitnypi*iksn*n*ch*f**ifivnvqikfltn
*tnlkqtknlfnkrrtsnq*firwkcsnpnfirlsskgkfiirlkgnr*iknifs*invp
nffitrlyfnfl*ftnspmhwk**pkv**yynq*llwd*kk*sixipgikinkg
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK791 (VHK791Q) /CSM/VH/VHK7-D/VHK791Q.Seq.d/                       1828   0.0
VHO809 (VHO809Q) /CSM/VH/VHO8-A/VHO809Q.Seq.d/                       1344   0.0
VHB767 (VHB767Q) /CSM/VH/VHB7-C/VHB767Q.Seq.d/                       1344   0.0
VHE123 (VHE123Q) /CSM/VH/VHE1-A/VHE123Q.Seq.d/                       1342   0.0
VHD239 (VHD239Q) /CSM/VH/VHD2-B/VHD239Q.Seq.d/                       1326   0.0
VHK810 (VHK810Q) /CSM/VH/VHK8-A/VHK810Q.Seq.d/                       1298   0.0
VFN211 (VFN211Q) /CSM/VF/VFN2-A/VFN211Q.Seq.d/                       1176   0.0
VHN647 (VHN647Q) /CSM/VH/VHN6-B/VHN647Q.Seq.d/                        581   e-164
VHE271 (VHE271Q) /CSM/VH/VHE2-C/VHE271Q.Seq.d/                        581   e-164
VHC102 (VHC102Q) /CSM/VH/VHC1-A/VHC102Q.Seq.d/                        581   e-164
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ443404) Dictyostelium discoideum cDNA clone:ddv52f23, 3' ...  1366   0.0    1
(BJ445598) Dictyostelium discoideum cDNA clone:ddv60b04, 3' ...  1328   0.0    2
(BJ437533) Dictyostelium discoideum cDNA clone:ddv34f17, 3' ...  1328   0.0    2
(BJ443141) Dictyostelium discoideum cDNA clone:ddv52d04, 3' ...  1275   0.0    2
(BJ439015) Dictyostelium discoideum cDNA clone:ddv39m05, 3' ...  1275   0.0    3
(BJ438600) Dictyostelium discoideum cDNA clone:ddv37m10, 3' ...  1275   0.0    3
(BJ416709) Dictyostelium discoideum cDNA clone:ddv27e04, 5' ...  1176   0.0    1
(BJ424548) Dictyostelium discoideum cDNA clone:ddv52f23, 5' ...   591   0.0    2
(BJ426269) Dictyostelium discoideum cDNA clone:ddv58m12, 5' ...   581   0.0    2
(BJ420557) Dictyostelium discoideum cDNA clone:ddv39m18, 5' ...   581   0.0    2
dna update 2008.11.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9NZJ4) RecName: Full=Sacsin;  &AL157766_4(AL157766|pid:none)        106   2e-21
CP001577_292(CP001577|pid:none) Micromonas sp. RCC299 chromosome...   105   3e-21
BC142916_1(BC142916|pid:none) Danio rerio hypothetical LOC555303...   105   5e-21
BC171956_1(BC171956|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   1e-20
BC138482_1(BC138482|pid:none) Mus musculus sacsin, mRNA (cDNA cl...   103   1e-20
(Q9JLC8) RecName: Full=Sacsin;                                        103   1e-20
AB006708_10(AB006708|pid:none) Arabidopsis thaliana genomic DNA,...   101   7e-20
AL732351_6(AL732351|pid:none) Oryza sativa genomic DNA, chromoso...    92   4e-17
AL606457_18(AL606457|pid:none) Oryza sativa genomic DNA, chromos...    90   2e-16
CR749427_1(CR749427|pid:none) Homo sapiens mRNA; cDNA DKFZp686B1...    75   7e-12
protein update 2009. 7.15
PSORT 

 psg: 0.55  gvh: 0.39  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: peroxisomal

>> prediction for VHK791 is cyt
5' end seq. ID VHK791F
5' end seq. 
>VHK791F.Seq
GATTAAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAGATAATCTTCTTAA
CTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAGATGCTCGAGCTGA
TACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGTTGTTAATTTCAAA
TGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAATTTTAATTTATAA
TAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTAGTCAAGGTAGTAA
AAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACAGTGTTTATCATAT
AACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAGATCCAAACGAAAG
AATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATAAGAAATGTTTTAG
TTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAAAAGAATATTTTAA
TGGTACAATCATTCGTCTACCATTGAGAATNNNNNNNNNN
Length of 5' end seq. 580
3' end seq. ID VHK791Z
3' end seq. 
>VHK791Z.Seq
NNNNNNNNNNCTCTTATTCAAATATTATGGTAATAAGTTGGCCAAACATTTAAAATTTTC
TACATTACCATTAAACTTTAGACAATTAATTTCATCAATTTGTAGAAATTATATAAATAT
TAGAGAAATTAAACTTTCAGATTTTATCACTAGTCTCAACTCCATCAACAACCTCCAATT
TTTAAGTGATAGTATAATAACAATTTGGAATTGTATCGATGAATTAAAAAGAAAAGAAAC
TTTAGAATTATCTCATATCCAATCATTACAAATTATCCCATATAGATTAAGTCCAACTGA
AACTGATGTCACTAGTTTTGATAAATCTTCATTGTTAATGTACAAATCAAATTTCTTACC
AATTGAACCAATCTTAAACAAACTAAAAATCTATTTAATAAAAGAAGGACATCAAATCAA
TAGTTTATTAGATGGAAATGCTCAAATCCCAATTTTATTAGGCTATCTTCAAAGGGTAAA
TTTATCATTAGACTCAAAGGAAATCGATAAATTAAGAATATTTTTAGTTGAATCAATGTT
CCAAATTTCTTTATCACAAGATTATACTTCAATTTTTTATAATTTACCAATTCACCCATG
CATTGGAAATAGTAACCAAAAGTTTAGTAGTATTACAACCAGTAGTTACTGTGGGATTAG
AAAAAATGATCAATANAAATTCCAGGNATTAAAATTAATAAAGGG
Length of 3' end seq. 705
Connected seq. ID VHK791P
Connected seq. 
>VHK791P.Seq
GATTAAAAATAAAAGTCAAACAATAAAACTATCTCATTTTTTAAGAGATAATCTTCTTAA
CTATCCAGAATCAAAAATATTTAGAGAATTGATTCAAAATGCAGAAGATGCTCGAGCTGA
TACAGTCATAATAAAGGTTGATGAAGGCTCATATCCAAATAATGGGTTGTTAATTTCAAA
TGATAACCAAGAAATTGAAAGTTCAAAAGAATTATTAGGTCCATCAATTTTAATTTATAA
TAATGCAACTTTTCAAGAACATGATTGGGAAGGAATTTGTCAAATTAGTCAAGGTAGTAA
AAAAGAAAATTTAAAAAGTGTTGGCAATTTCGGTTTAGGTTGGAACAGTGTTTATCATAT
AACAGATAATCCAATTGTTTATAGTGGTAATAATGTTTGGTTCTCAGATCCAAACGAAAG
AATGAGTGGTGGTTTATTCTTTGATTTAGATGACGAAGATGATTATAAGAAATGTTTTAG
TTTTTTAGAGCCATTCATTCAATTTAAAGAATCAAATTGTAACCCAAAAGAATATTTTAA
TGGTACAATCATTCGTCTACCATTGAGAAT----------CTCTTATTCAAATATTATGG
TAATAAGTTGGCCAAACATTTAAAATTTTCTACATTACCATTAAACTTTAGACAATTAAT
TTCATCAATTTGTAGAAATTATATAAATATTAGAGAAATTAAACTTTCAGATTTTATCAC
TAGTCTCAACTCCATCAACAACCTCCAATTTTTAAGTGATAGTATAATAACAATTTGGAA
TTGTATCGATGAATTAAAAAGAAAAGAAACTTTAGAATTATCTCATATCCAATCATTACA
AATTATCCCATATAGATTAAGTCCAACTGAAACTGATGTCACTAGTTTTGATAAATCTTC
ATTGTTAATGTACAAATCAAATTTCTTACCAATTGAACCAATCTTAAACAAACTAAAAAT
CTATTTAATAAAAGAAGGACATCAAATCAATAGTTTATTAGATGGAAATGCTCAAATCCC
AATTTTATTAGGCTATCTTCAAAGGGTAAATTTATCATTAGACTCAAAGGAAATCGATAA
ATTAAGAATATTTTTAGTTGAATCAATGTTCCAAATTTCTTTATCACAAGATTATACTTC
AATTTTTTATAATTTACCAATTCACCCATGCATTGGAAATAGTAACCAAAAGTTTAGTAG
TATTACAACCAGTAGTTACTGTGGGATTAGAAAAAATGATCAATANAAATTCCAGGNATT
AAAATTAATAAAGGG
Length of connected seq. 1265
Full length Seq ID -
Full length Seq. -
Length of full length seq. -