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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK669
Library VH
(Link to library)
Clone ID VHK669
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHK669P
(Link to Original site)
Representative DNA sequence 
>VHK669 (VHK669Q) /CSM/VH/VHK6-C/VHK669Q.Seq.d/
GTTGATCCAATTGAAGTAGCACAAAAAGTAGTTAGTGGAGTATTTCCAGGTGTAAAGACT
ACAGAATTGGATAATTTGGCAGCCGAGACAGCAGCATATATGTCAACACGTCATCCAGAT
TATGGTATTTTGGCAGCACGTATTAGTGTATCGAATCTTCATAAACAAACTTCAAAGAGT
TTTGTAGAGACAGTTAAGAAACAATATGAATTCATTAATCCAAAGAGCAATTTATTGGCA
CCATTGGTCAGCAAGGAGCTCTATGAAATCGTAATGAAACATGGTGAGAGATTGGAGAGT
GTCATTGATTATTATCGTGATTTCGATTACGATTTCTTTGGATTCAAAACATTGGAACGT
TCCTATCTTCATCGTATCAATGGTAAAATTGTCGAGCGTCCACAACACATGTTGATGCGT
GTCTCCATTGGTATCCATGGCGAAGACCTCGAATCTGCCATCAACACCTACAAACGTTTG
TCTGAGAAATTGTTCACTCATGCCACACCAACTCTATTCAACGCAGGTACACCATCACCA
CAAATGTCATCTTGTTTCTTGGTTCAAATGAAGGAGGATTCAATCGATGGTATTTATGAC
ACTTTGAAACAATGTGCACTCATTTCAAXXXXXXXXXXCAAGTCAAATCCTTGGTAGCAA
TGAATGCTNTGAACNTCACACTTCCAACATTTACAGTCGTCGTGTTTTAGCAGGTGAATT
CACAATTGTCANCGAACAATTACTCGAAGATNTCATGGAATTGGGCATNTGGTCACCANA
GATGAAGAATCAAATCGTTGTTGCACGTGGTTCCATTCAATCCATCGGTGGCATCCAATN
ATGATTTGAAAGAACTCTATAAAACAGTTTGGGAAATCCGTCAAAGAACTCTCATCGATA
TGGCTGCTGATCGTGGTGCTTTCATCGATCAAAGTCAAAGTTTCAATGTTTTCATNGNTG
AACCAACCTTTGNTAAACTCACCTCAATGCATTTNTACTCATGGAAGAAAGGTTTAAAGA
CATGGTATGTACTATCTTCGTANTCGTCCTGCCGCTGATGCAATTCAATTCANTGTTGAT
CCAATCGTATCTCAAACTCCAAAACCAGTTGAAAATAATATAAACTCANNTACACCACTT
AAAACTCCAANTAAAACTCCAAACTCTTCAAATCGTATCTCTACTAGTCCAACTAATAAT
TTAACAAGTCCAATTCGTTTTACATATAACNCAACAACAACAACANCAACAACAACAACA
ACAACAACAAAATAATAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
VDPIEVAQKVVSGVFPGVKTTELDNLAAETAAYMSTRHPDYGILAARISVSNLHKQTSKS
FVETVKKQYEFINPKSNLLAPLVSKELYEIVMKHGERLESVIDYYRDFDYDFFGFKTLER
SYLHRINGKIVERPQHMLMRVSIGIHGEDLESAINTYKRLSEKLFTHATPTLFNAGTPSP
QMSSCFLVQMKEDSIDGIYDTLKQCALIS---

---SQILGSNECXEXHTSNIYSRRVLAGEFTIVXEQLLEDXMELGXWSPXMKNQIVVARG
SIQSIGGIQX*fertl*nslgnpsknshrygc*swcfhrskskfqcfhx*tnlx*thlna
fxlmeerfkdmvctifvxvlplmqfnsxliqsylklqnqlkii*txxhhlklqxklqtlq
ivsllvqlii*qvqfvlhitqqqqxqqqqqqqqnnk
Translated Amino Acid sequence (All Frames) 
Frame A:
VDPIEVAQKVVSGVFPGVKTTELDNLAAETAAYMSTRHPDYGILAARISVSNLHKQTSKS
FVETVKKQYEFINPKSNLLAPLVSKELYEIVMKHGERLESVIDYYRDFDYDFFGFKTLER
SYLHRINGKIVERPQHMLMRVSIGIHGEDLESAINTYKRLSEKLFTHATPTLFNAGTPSP
QMSSCFLVQMKEDSIDGIYDTLKQCALIS---

---qvkslvamnaxnxtlptftvvvf*qvnsqlsxnnyskxswnwaxghxr*riksllhv
vpfnpsvasnxdlkelyktvweirqrtlidmaadrgafidqsqsfnvfxxeptfxkltsm
hxyswkkglktwyvlssxsscr*cnsixc*snrisnskts*k*yklxytt*nsx*nsklf
ksyly*sn**fnksnsfyi*xnnnnxnnnnnnnkii

Frame B:
liqlk*hkk*lveyfqv*rlqnwiiwqprqqhicqhviqimvfwqhvlvyrifinklqrv
l*rqlrnnmnsliqraiywhhwsarssmks**nmvrdwrvsliiivisitisldskhwnv
pifivsmvklssvhntc*cvsplvsmaktsnlpstptnvclrncslmphqlystqvhhhh
kchlvswfk*rriqsmvfmtl*nnvhsfq---

---ksnpw*q*ml*tshfqhlqsscfsr*ihncxrtitrrxhgighxvtxdeesnrcctw
fhsihrwhpxmi*knsikqfgksvkelssiwllivvlssikvkvsmfsxxnqplxnspqc
ixthgrkv*rhgmyylrxrpaadaiqfxvdpivsqtpkpvenninsxtplktpxktpnss
nristsptnnltspirftynxttttxtttttttk**

Frame C:
*sn*sstkss*wsisrckdyrig*fgsrdssiyvntssrlwyfgsty*ciess*tnfkef
crds*eti*ih*skeqfigtigqqgal*nrnetw*eigech*lls*frlrflwiqnigtf
lsssyqw*ncrastthvdaclhwypwrrprichqhlqtfv*eivhschtnsiqrrytitt
nvilflgsneggfnrwyl*hfetmcthf---

---SQILGSNECXEXHTSNIYSRRVLAGEFTIVXEQLLEDXMELGXWSPXMKNQIVVARG
SIQSIGGIQX*fertl*nslgnpsknshrygc*swcfhrskskfqcfhx*tnlx*thlna
fxlmeerfkdmvctifvxvlplmqfnsxliqsylklqnqlkii*txxhhlklqxklqtlq
ivsllvqlii*qvqfvlhitqqqqxqqqqqqqqnnk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK669 (VHK669Q) /CSM/VH/VHK6-C/VHK669Q.Seq.d/                       2278   0.0
CHJ148 (CHJ148Q) /CSM/CH/CHJ1-B/CHJ148Q.Seq.d/                       1245   0.0
VHF168 (VHF168Q) /CSM/VH/VHF1-C/VHF168Q.Seq.d/                       1126   0.0
CHF205 (CHF205Q) /CSM/CH/CHF2-A/CHF205Q.Seq.d/                       1057   0.0
VHD476 (VHD476Q) /CSM/VH/VHD4-D/VHD476Q.Seq.d/                       1037   0.0
SHB724 (SHB724Q) /CSM/SH/SHB7-A/SHB724Q.Seq.d/                       1027   0.0
AHF429 (AHF429Q) /CSM/AH/AHF4-B/AHF429Q.Seq.d/                       1027   0.0
CHR614 (CHR614Q) /CSM/CH/CHR6-A/CHR614Q.Seq.d/                       1015   0.0
AHI672 (AHI672Q) /CSM/AH/AHI6-C/AHI672Q.Seq.d/                       1007   0.0
SHB485 (SHB485Q) /CSM/SH/SHB4-D/SHB485Q.Seq.d/                        995   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ424494) Dictyostelium discoideum cDNA clone:ddv52i18, 5' ...  1245   0.0    1
(BJ366845) Dictyostelium discoideum cDNA clone:ddc40o11, 5' ...  1245   0.0    1
(BJ421060) Dictyostelium discoideum cDNA clone:ddv41g17, 5' ...  1126   0.0    1
(BJ443336) Dictyostelium discoideum cDNA clone:ddv52i18, 3' ...  1078   0.0    1
(BJ364545) Dictyostelium discoideum cDNA clone:ddc32i02, 5' ...  1057   0.0    1
(BJ419979) Dictyostelium discoideum cDNA clone:ddv37h20, 5' ...  1037   0.0    1
(BJ392318) Dictyostelium discoideum cDNA clone:dds27p05, 5' ...  1027   0.0    1
(BJ350899) Dictyostelium discoideum cDNA clone:dda41j08, 3' ...   664   0.0    2
(BJ386097) Dictyostelium discoideum cDNA clone:ddc57k04, 3' ...   662   0.0    2
(BJ353020) Dictyostelium discoideum cDNA clone:dda48o18, 3' ...   686   0.0    2
dna update 2008.11.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54Q71) RecName: Full=Ribonucleoside-diphosphate reductase larg...   381   e-104
S34807(S34807) ribonucleoside-diphosphate reductase (EC 1.17.4.1...   282   2e-74
AM270068_2(AM270068|pid:none) Aspergillus niger contig An04c0070...   280   8e-74
CR382139_247(CR382139|pid:none) Debaryomyces hansenii strain CBS...   272   2e-71
AM920437_1772(AM920437|pid:none) Penicillium chrysogenum Wiscons...   271   3e-71
AE016817_343(AE016817|pid:none) Ashbya gossypii (= Eremothecium ...   268   2e-70
CU638744_79(CU638744|pid:none) Podospora anserina genomic DNA ch...   265   3e-69
AM437010_1(AM437010|pid:none) Vitis vinifera contig VV78X108426....   263   1e-68
CU928165_187(CU928165|pid:none) Kluyveromyces thermotolerans str...   262   2e-68
(P21524) RecName: Full=Ribonucleoside-diphosphate reductase larg...   262   2e-68
protein update 2009. 7.15
PSORT 

 psg: 0.75  gvh: 0.39  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VHK669 is cyt
5' end seq. ID VHK669F
5' end seq. 
>VHK669F.Seq
GTTGATCCAATTGAAGTAGCACAAAAAGTAGTTAGTGGAGTATTTCCAGGTGTAAAGACT
ACAGAATTGGATAATTTGGCAGCCGAGACAGCAGCATATATGTCAACACGTCATCCAGAT
TATGGTATTTTGGCAGCACGTATTAGTGTATCGAATCTTCATAAACAAACTTCAAAGAGT
TTTGTAGAGACAGTTAAGAAACAATATGAATTCATTAATCCAAAGAGCAATTTATTGGCA
CCATTGGTCAGCAAGGAGCTCTATGAAATCGTAATGAAACATGGTGAGAGATTGGAGAGT
GTCATTGATTATTATCGTGATTTCGATTACGATTTCTTTGGATTCAAAACATTGGAACGT
TCCTATCTTCATCGTATCAATGGTAAAATTGTCGAGCGTCCACAACACATGTTGATGCGT
GTCTCCATTGGTATCCATGGCGAAGACCTCGAATCTGCCATCAACACCTACAAACGTTTG
TCTGAGAAATTGTTCACTCATGCCACACCAACTCTATTCAACGCAGGTACACCATCACCA
CAAATGTCATCTTGTTTCTTGGTTCAAATGAAGGAGGATTCAATCGATGGTATTTATGAC
ACTTTGAAACAATGTGCACTCATTTCAANNNNNNNNNN
Length of 5' end seq. 638
3' end seq. ID VHK669Z
3' end seq. 
>VHK669Z.Seq
NNNNNNNNNNCAAGTCAAATCCTTGGTAGCAATGAATGCTNTGAACNTCACACTTCCAAC
ATTTACAGTCGTCGTGTTTTAGCAGGTGAATTCACAATTGTCANCGAACAATTACTCGAA
GATNTCATGGAATTGGGCATNTGGTCACCANAGATGAAGAATCAAATCGTTGTTGCACGT
GGTTCCATTCAATCCATCGGTGGCATCCAATNATGATTTGAAAGAACTCTATAAAACAGT
TTGGGAAATCCGTCAAAGAACTCTCATCGATATGGCTGCTGATCGTGGTGCTTTCATCGA
TCAAAGTCAAAGTTTCAATGTTTTCATNGNTGAACCAACCTTTGNTAAACTCACCTCAAT
GCATTTNTACTCATGGAAGAAAGGTTTAAAGACATGGTATGTACTATCTTCGTANTCGTC
CTGCCGCTGATGCAATTCAATTCANTGTTGATCCAATCGTATCTCAAACTCCAAAACCAG
TTGAAAATAATATAAACTCANNTACACCACTTAAAACTCCAANTAAAACTCCAAACTCTT
CAAATCGTATCTCTACTAGTCCAACTAATAATTTAACAAGTCCAATTCGTTTTACATATA
ACNCAACAACAACAACANCAACAACAACAACAACAACAACAAAATAATAAG
Length of 3' end seq. 651
Connected seq. ID VHK669P
Connected seq. 
>VHK669P.Seq
GTTGATCCAATTGAAGTAGCACAAAAAGTAGTTAGTGGAGTATTTCCAGGTGTAAAGACT
ACAGAATTGGATAATTTGGCAGCCGAGACAGCAGCATATATGTCAACACGTCATCCAGAT
TATGGTATTTTGGCAGCACGTATTAGTGTATCGAATCTTCATAAACAAACTTCAAAGAGT
TTTGTAGAGACAGTTAAGAAACAATATGAATTCATTAATCCAAAGAGCAATTTATTGGCA
CCATTGGTCAGCAAGGAGCTCTATGAAATCGTAATGAAACATGGTGAGAGATTGGAGAGT
GTCATTGATTATTATCGTGATTTCGATTACGATTTCTTTGGATTCAAAACATTGGAACGT
TCCTATCTTCATCGTATCAATGGTAAAATTGTCGAGCGTCCACAACACATGTTGATGCGT
GTCTCCATTGGTATCCATGGCGAAGACCTCGAATCTGCCATCAACACCTACAAACGTTTG
TCTGAGAAATTGTTCACTCATGCCACACCAACTCTATTCAACGCAGGTACACCATCACCA
CAAATGTCATCTTGTTTCTTGGTTCAAATGAAGGAGGATTCAATCGATGGTATTTATGAC
ACTTTGAAACAATGTGCACTCATTTCAA----------CAAGTCAAATCCTTGGTAGCAA
TGAATGCTNTGAACNTCACACTTCCAACATTTACAGTCGTCGTGTTTTAGCAGGTGAATT
CACAATTGTCANCGAACAATTACTCGAAGATNTCATGGAATTGGGCATNTGGTCACCANA
GATGAAGAATCAAATCGTTGTTGCACGTGGTTCCATTCAATCCATCGGTGGCATCCAATN
ATGATTTGAAAGAACTCTATAAAACAGTTTGGGAAATCCGTCAAAGAACTCTCATCGATA
TGGCTGCTGATCGTGGTGCTTTCATCGATCAAAGTCAAAGTTTCAATGTTTTCATNGNTG
AACCAACCTTTGNTAAACTCACCTCAATGCATTTNTACTCATGGAAGAAAGGTTTAAAGA
CATGGTATGTACTATCTTCGTANTCGTCCTGCCGCTGATGCAATTCAATTCANTGTTGAT
CCAATCGTATCTCAAACTCCAAAACCAGTTGAAAATAATATAAACTCANNTACACCACTT
AAAACTCCAANTAAAACTCCAAACTCTTCAAATCGTATCTCTACTAGTCCAACTAATAAT
TTAACAAGTCCAATTCGTTTTACATATAACNCAACAACAACAACANCAACAACAACAACA
ACAACAACAAAATAATAAG
Length of connected seq. 1269
Full length Seq ID -
Full length Seq. -
Length of full length seq. -