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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO433
Library AF
(Link to library)
Clone ID AFO433
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10734-1
Original site URL
Representative seq. ID AFO433Z
(Link to Original site)
Representative DNA sequence 
>AFO433 (AFO433Q) /CSM/AF/AFO4-B/AFO433Q.Seq.d/
XXXXXXXXXXTAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTC
GATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCA
GCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACC
TTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCA
TTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGT
ACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCT
CCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTA
AAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGT
GATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAAT
CCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGAT
AACGCTGCCATGAATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTAAAAA
AGATTCTTCTGCTAAAC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---KKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETF
SFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAP
WCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENP
ISYEGQRNDHMNFVEFIQDNAAMNSNYHHLKLMIMXI*krffc*
Translated Amino Acid sequence (All Frames) 
Frame A:
---kenryltkr*crfllgryeeipttsnsygslw*scsshlc*fscq*sslqfr*krnl
liryrlqmdprchww*sfsic*istrtrik*css*gcrwyhfqkvgfrltkgcfg*ilrs
mvrslqescsnlr*ir*ilkrr*iclnrkd*crlk*ctk*yrn*rlpnhhalqs**qrks
nll*rskk*syelc*ihsr*rchefklpssqtddnvnlkkillln


Frame B:
---KKIATSQKGNAVFCWVDMKKFPQQATHMGLSGKVVPAISVDSVANKARYNFDEKETF
SFDTVSKWIQDVIGGKVSPFVKSQPEPESNDAPVKVAVGTTFKKLVLDSPKDVLVEFYAP
WCGHCKNLAPIYDKLGEYLKDVESVSIVKIDADSNDVPSDIEIRGYPTIMLFKADDKENP
ISYEGQRNDHMNFVEFIQDNAAMNSNYHHLKLMIMXI*krffc*


Frame C:
---rkslphkkvmpfsvgsi*rnshnkqliwvslvklfqpslliqlpiklvtismkkkps
hsipspngskmslvvkflhllnlnqnqnqmmlqlrlplvplskswf*thqrmfwlnstlh
gaviarillqstin*vnt*ktlnlsqs*rlmptqmmyqvisklevtqpscssklmtkkiq
slmkvkemii*tllnsfkitlp*iqitiisn***cxskkdssak
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO433 (AFO433Q) /CSM/AF/AFO4-B/AFO433Q.Seq.d/                       1316   0.0
AFM548 (AFM548Q) /CSM/AF/AFM5-B/AFM548Q.Seq.d/                       1316   0.0
AFM212 (AFM212Q) /CSM/AF/AFM2-A/AFM212Q.Seq.d/                       1316   0.0
AFK539 (AFK539Q) /CSM/AF/AFK5-B/AFK539Q.Seq.d/                       1316   0.0
AFK217 (AFK217Q) /CSM/AF/AFK2-A/AFK217Q.Seq.d/                       1316   0.0
AFK269 (AFK269Q) /CSM/AF/AFK2-C/AFK269Q.Seq.d/                       1310   0.0
AFJ487 (AFJ487Q) /CSM/AF/AFJ4-D/AFJ487Q.Seq.d/                       1310   0.0
AFM717 (AFM717Q) /CSM/AF/AFM7-A/AFM717Q.Seq.d/                       1308   0.0
AFJ720 (AFJ720Q) /CSM/AF/AFJ7-A/AFJ720Q.Seq.d/                       1306   0.0
SFL246 (SFL246Q) /CSM/SF/SFL2-B/SFL246Q.Seq.d/                       1302   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BJ171852|BJ171852.1 Physcomitrella patens subsp. patens cDNA clone:pph30f12, 3' end, single read.   599  e-167  1
BM398179|BM398179.1 5009-0-41-G11.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    74  2e-09  1
AL924030|AL924030.1 Danio rerio EST sequence, clone 134-B07-2.    70  4e-08  1
CB176061|CB176061.1 pl17e07.y3 Ancylostoma ceylanicum adult Ancylostoma ceylanicum cDNA 5' similar to TR:O17908 O17908 H06O01.1 PROTEIN. [1] ;, mRNA sequence.    70  4e-08  1
BM280519|BM280519.1 ki01h03.y1 Ascaris suum L4 pSPORT1 Zarlenga v1 Ascaris suum cDNA 5' similar to SW:ERP5_CAEEL Q11067 PROBABLE PROTEIN DISULFIDE ISOMERASE P5 PRECURSOR ;, mRNA sequence.    68  2e-07  1
AW255071|AW255071.1 ML1365 peppermint glandular trichome Mentha x piperita cDNA, mRNA sequence.    64  2e-06  1
BM315730|BM315730.1 fw64d09.y1 Sugano SJD adult male Danio rerio cDNA clone 5612584 5' similar to SW:ER60_HUMAN P30101 PROBABLE PROTEIN DISULFIDE ISOMERASE ER-60 PRECURSOR ;, mRNA sequence.    62  9e-06  1
BJ576792|BJ576792.1 Ipomoea nil cDNA clone:jm33b20, 3' end, single read.    60  4e-05  1
BM308748|BM308748.1 sak49h10.y1 Gm-c1036 Glycine max cDNA clone SOYBEAN CLONE ID: Gm-c1036-6523 5' similar to TR:P93358 P93358 PROTEIN DISULFIDE-ISOMERASE PRECURSOR. ;, mRNA sequence.    60  4e-05  1
BU795021|S34275 protein disulfide-isomerase homolog precursor - fluke  (Schistosoma mansoni), mRNA sequence.    60  4e-05  1
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q12730) RecName: Full=Protein disulfide-isomerase;          Sho...   169   5e-41
EU016183_1(EU016183|pid:none) Haemaphysalis longicornis strain G...   169   5e-41
(Q00248) RecName: Full=Protein disulfide-isomerase;          Sho...   166   4e-40
DQ066336_1(DQ066336|pid:none) Ixodes scapularis isolate IS-6-12-...   165   1e-39
CU633901_146(CU633901|pid:none) Podospora anserina genomic DNA c...   160   3e-38
DQ377176_1(DQ377176|pid:none) Amblyomma variegatum protein disul...   160   4e-38
AE013599_1413(AE013599|pid:none) Drosophila melanogaster chromos...   151   2e-35
AK290971_1(AK290971|pid:none) Homo sapiens cDNA FLJ78033 complet...   150   2e-35
BC046707_1(BC046707|pid:none) Xenopus laevis glucose regulated p...   150   2e-35
(P13667) RecName: Full=Protein disulfide-isomerase A4;          ...   150   2e-35
protein update 2009. 4.26
PSORT 

 psg: 0.86  gvh: 0.36  alm: 0.43  top: 0.47  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for AFO433 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFO433Z
3' end seq. 
>AFO433Z.Seq
----------TAAAGAAAATCGCTACCTCACAAAAAGGTAATGCCGTTTTCTGTTGGGTC
GATATGAAGAAATTCCCACAACAAGCAACTCATATGGGTCTCTCTGGTAAAGTTGTTCCA
GCCATCTCTGTTGATTCAGTTGCCAATAAAGCTCGTTACAATTTCGATGAAAAAGAAACC
TTCTCATTCGATACCGTCTCCAAATGGATCCAAGATGTCATTGGTGGTAAAGTTTCTCCA
TTTGTTAAATCTCAACCAGAACCAGAATCAAATGATGCTCCAGTTAAGGTTGCCGTTGGT
ACCACTTTCAAAAAGTTGGTTTTAGACTCACCAAAGGATGTTTTGGTTGAATTCTACGCT
CCATGGTGCGGTCATTGCAAGAATCTTGCTCCAATCTACGATAAATTAGGTGAATACTTA
AAAGACGTTGAATCTGTCTCAATCGTAAAGATTGATGCCGACTCAAATGATGTACCAAGT
GATATCGAAATTAGAGGTTACCCAACCATCATGCTCTTCAAAGCTGATGACAAAGAAAAT
CCAATCTCTTATGAAGGTCAAAGAAATGATCATATGAACTTTGTTGAATTCATTCAAGAT
AACGCTGCCATGAATTCAAATTACCATCATCTCAAACTGATGATAATGTNAATCTAAAAA
AGATTCTTCTGCTAAAC
Length of 3' end seq. 667
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -