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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO416
Library AF
(Link to library)
Clone ID AFO416
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16272-1
Original site URL
Representative seq. ID AFO416P
(Link to Original site)
Representative DNA sequence 
>AFO416 (AFO416Q) /CSM/AF/AFO4-A/AFO416Q.Seq.d/
GTTAGTCACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTC
ACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGT
CATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAG
CTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAG
AAATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTG
GTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAA
GTGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTT
TCACCAAATACGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTC
TCCAAGCCATCAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACA
AATTAAAATTAACTCAAAAGAAAGCACACGTCCTTGAAATCCAAGTCAATGGTGGTTCAA
TCGTCGAAAAAGTTAACTTTGCTGTTGCCAACTTTGAAAAGACCGTCAACGTCACTGGTG
TCTTTGCCGAAAATGAATTAATCGATGTTATXXXXXXXXXXCTCAACCGGTAAGACTGAA
TTCGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTNGCTCACTACGGTATGGTT
AAACATGAATTTTTAATGCTTAAAGGTATGTTATTTAATTTGNATAAATTATTATTATTT
TTTTTTTATTATTATGTTTTTCTTTTTTCCATAATATTGTTAGATTTCTCCTTTGCTTTT
TCATTTTTTTCACTTCCTATTATGTTTAAGTTCTTTCTGAAAATGCTATTTTTTTTTCCC
CCATTTATTGTTATTTTTTTTCTTTTTCTTTTTTTATTATTATTATTATCATTATTATTA
TATAATTATACGATTCAAATACTAATATATAATTTTCAATAAATAGGTTGTGTTGCTGGT
CCAAGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCC
CTCGAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCAT
CACAACCGCTGCAAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAGG
GCTTAACATTCAATAAATTGATTTCA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
*SLLNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKA
GMTHVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLS
DNFRRRLYKNWYKSKSKKAFTKYVKQYETEEGKKSIEASLQAIKKRCSVVRVIAHTQVHK
LKLTQKKAHVLEIQVNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDV---

---STGKTEFDLTEKTINPMGGXAHYGMVKHEFLMLKGMLFNLXKLLLFFFYYYVFLFSI
ILLDFSFAFSFFSLPIMFKFFLKMLFFFPPFIVIFFLFLFLLLLLSLLLYNYTIQILIYN
FQ*igcvagprkraltlrksittqtgraalekitlkfidtsskfghglhhnrckirlnis
asrnqdqlra*hsin*f
Translated Amino Acid sequence (All Frames) 
Frame A:
vshf*ttiti*vivnlklhvtvisvsdqereppdikvksshsqrmtvpkkst*lhswvtk
lv*ltlsei*knqvqrctkkkslkpspslnvhqctllv*lvmlkplkvskptkpsglni*
vitsvedytktgtnqnqrklspntlnntklkkvknqlkplskpskrdvqssvslltlkft
n*n*lkrkhtslksksmvvqsskkltlllptlkrpstslvslpkmn*sml---

---lnr*d*irfn*kdyqsngwfxslryg*t*ifna*ryvi*fx*iiiifflllcfsffh
nivrfllcffifftsyyv*vlsenaiffspiycyffsfsffiiiiiiiii*lydsntni*
fsinrlccwskkeslnlkkeyhhsnwsccprenhsqihrylqqvwsrsssqplqdktkyf
gvkksrstkgltfnklis

Frame B:
lvtskqq*qyess*i*sstsr*srfqtkkesrqtsr*sqvipkg*pypksplncihglqs
wydsrcqrfrktrfkdaqkrnr*srhhh*mstnvhcwfswlc*nrsrsqnlqnrlgstfk
**lp*kiiqklvqikikesfhqir*tirn*rr*kin*slspshqkemfsrpchcshsssq
ikinskestrp*npsqwwfnrrks*lcccql*kdrqrhwclcrk*inrcy---

---STGKTEFDLTEKTINPMGGXAHYGMVKHEFLMLKGMLFNLXKLLLFFFYYYVFLFSI
ILLDFSFAFSFFSLPIMFKFFLKMLFFFPPFIVIFFLFLFLLLLLSLLLYNYTIQILIYN
FQ*igcvagprkraltlrksittqtgraalekitlkfidtsskfghglhhnrckirlnis
asrnqdqlra*hsin*f

Frame C:
*SLLNNNNNMSHRKFEAPRHGNLGFRPRKRAARHQGKVKSFPKDDRTQKVHLTAFMGYKA
GMTHVVRDLEKPGSKMHKKEIVEAVTIIECPPMYIVGLVGYVETAQGLKTYKTVWAQHLS
DNFRRRLYKNWYKSKSKKAFTKYVKQYETEEGKKSIEASLQAIKKRCSVVRVIAHTQVHK
LKLTQKKAHVLEIQVNGGSIVEKVNFAVANFEKTVNVTGVFAENELIDV---

---qpvrlnsi*lkrlsiqwvvxlttvwlnmnf*clkvcylixinyyyfffiimffffp*
yc*ispllfhffhfllclssf*kcyfffphlllfffffffyyyyyhyyyiiirfky*yii
fnk*vvllvqerep*p*ervsplklvvlpsrkslsnssippaslvtvfittaar*d*ifr
rqeikin*glniq*idf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO416 (AFO416Q) /CSM/AF/AFO4-A/AFO416Q.Seq.d/                       1588   0.0
VFI511 (VFI511Q) /CSM/VF/VFI5-A/VFI511Q.Seq.d/                       1370   0.0
VFB816 (VFB816Q) /CSM/VF/VFB8-A/VFB816Q.Seq.d/                       1370   0.0
CFF138 (CFF138Q) /CSM/CF/CFF1-B/CFF138Q.Seq.d/                       1370   0.0
AFA535 (AFA535Q) /CSM/AF/AFA5-B/AFA535Q.Seq.d/                       1370   0.0
VFM836 (VFM836Q) /CSM/VF/VFM8-B/VFM836Q.Seq.d/                       1364   0.0
VFD340 (VFD340Q) /CSM/VF/VFD3-B/VFD340Q.Seq.d/                       1364   0.0
VFD212 (VFD212Q) /CSM/VF/VFD2-A/VFD212Q.Seq.d/                       1364   0.0
SFG613 (SFG613Q) /CSM/SF/SFG6-A/SFG613Q.Seq.d/                       1364   0.0
VFH212 (VFH212Q) /CSM/VF/VFH2-A/VFH212Q.Seq.d/                       1362   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

L08391|L08391.1 Dictyostelium discoideum ribosomal protein (L3) gene, complete cds.  1306  0.0  6
AX489150|AX489150.1 Sequence 6450 from Patent WO02053728.         42  1e-26  8
BE641342|BE641342.1 Cri2_3_A04_SP6 Ceratopteris Spore Library Ceratopteris richardii cDNA clone Cri2_3_A04 5', mRNA sequence.    76  1e-21  3
BM599962|BM599962.1 17000687050693 A.Gam.ad.cDNA.blood1 Anopheles gambiae cDNA clone 19600449725551 5', mRNA sequence.    58  2e-18  6
J01351|J01351.1 S.cerevisiae ribosomal protein L3 (tcm1) gene.    46  2e-18  6
Z74971|Z74971.1 S.cerevisiae chromosome XV reading frame ORF YOR063w.    46  2e-17  6
AL398773|AL398773.1 T7 end of clone AS0AA011B09 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.    46  2e-16  6
BQ622709|BQ622709.1 CC_Contig5 Conidiobolus cornatus ARSEF 512 Conidiobolus coronatus cDNA, mRNA sequence.    62  2e-16  4
AL436296|AL436296.1 T3 end of clone BC0AA002E09 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    40  2e-15  7
AL436279|AL436279.1 T3 end of clone BC0AA002D12 of library BC0AA from strain CBS 767 of Debaryomyces hansenii.    40  3e-15  7
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34113) RecName: Full=60S ribosomal protein L3;  &JC4254(JC4254...   444   e-123
EF144245_1(EF144245|pid:none) Populus trichocarpa clone PX0011_L...   309   2e-82
AM471157_1(AM471157|pid:none) Vitis vinifera contig VV78X211407....   309   2e-82
EF146052_1(EF146052|pid:none) Populus trichocarpa clone WS0115_D...   308   3e-82
EF145479_1(EF145479|pid:none) Populus trichocarpa clone WS01125_...   307   6e-82
(P22738) RecName: Full=60S ribosomal protein L3-2;  &AC005850_7(...   306   1e-81
DQ241849_1(DQ241849|pid:none) Solanum tuberosum clone 187E04 rib...   305   2e-81
AC125473_1(AC125473|pid:none) Medicago truncatula clone mth2-8j1...   305   2e-81
(P36584) RecName: Full=60S ribosomal protein L3-B;  &AL590902_6(...   305   2e-81
JQ0772(JQ0772) ribosomal protein L3.e (clone ARP2), cytosolic - ...   305   3e-81
protein update 2009. 4.26
PSORT 

 psg: 0.60  gvh: 0.20  alm: 0.07  top: 0.57  tms: 0.27  mit: 0.37  mip: 0.06
 nuc: 0.18  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 52.0 %: plasma membrane
	 16.0 %: vacuolar
	 12.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: endoplasmic reticulum
	  4.0 %: Golgi

>> prediction for AFO416 is pla
5' end seq. ID AFO416F
5' end seq. 
>AFO416F.Seq
GTTAGTCACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTC
ACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGT
CATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAG
CTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAG
AAATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTG
GTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAA
GTGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTT
TCACCAAATACGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTC
TCCAAGCCATCAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACA
AATTAAAATTAACTCAAAAGAAAGCACACGTCCTTGAAATCCAAGTCAATGGTGGTTCAA
TCGTCGAAAAAGTTAACTTTGCTGTTGCCAACTTTGAAAAGACCGTCAACGTCACTGGTG
TCTTTGCCGAAAATGAATTAATCGATGTTAT----------
Length of 5' end seq. 691
3' end seq. ID AFO416Z
3' end seq. 
>AFO416Z.Seq
----------CTCAACCGGTAAGACTGAATTCGATTTAACTGAAAAGACTATCAATCCAA
TGGGTGGTTTNGCTCACTACGGTATGGTTAAACATGAATTTTTAATGCTTAAAGGTATGT
TATTTAATTTGNATAAATTATTATTATTTTTTTTTTATTATTATGTTTTTCTTTTTTCCA
TAATATTGTTAGATTTCTCCTTTGCTTTTTCATTTTTTTCACTTCCTATTATGTTTAAGT
TCTTTCTGAAAATGCTATTTTTTTTTCCCCCATTTATTGTTATTTTTTTTCTTTTTCTTT
TTTTATTATTATTATTATCATTATTATTATATAATTATACGATTCAAATACTAATATATA
ATTTTCAATAAATAGGTTGTGTTGCTGGTCCAAGAAAGAGAGCCTTAACCTTAAGAAAGA
GTATCACCACTCAAACTGGTCGTGCTGCCCTCGAGAAAATCACTCTCAAATTCATCGATA
CCTCCAGCAAGTTTGGTCACGGTCTTCATCACAACCGCTGCAAGATAAGACTAAATATTT
CGGCGTCAAGAAATCAAGATCAACTAAGGGCTTAACATTCAATAAATTGATTTCA
Length of 3' end seq. 585
Connected seq. ID AFO416P
Connected seq. 
>AFO416P.Seq
GTTAGTCACTTCTAAACAACAATAACAATATGAGTCATCGTAAATTTGAAGCTCCACGTC
ACGGTAATCTCGGTTTCAGACCAAGAAAGAGAGCCGCCAGACATCAAGGTAAAGTCAAGT
CATTCCCAAAGGATGACCGTACCCAAAAAGTCCACTTAACTGCATTCATGGGTTACAAAG
CTGGTATGACTCACGTTGTCAGAGATTTAGAAAAACCAGGTTCAAAGATGCACAAAAAAG
AAATCGTTGAAGCCGTCACCATCATTGAATGTCCACCAATGTACATTGTTGGTTTAGTTG
GTTATGTTGAAACCGCTCAAGGTCTCAAAACCTACAAAACCGTCTGGGCTCAACATTTAA
GTGATAACTTCCGTAGAAGATTATACAAAAACTGGTACAAATCAAAATCAAAGAAAGCTT
TCACCAAATACGTTAAACAATACGAAACTGAAGAAGGTAAAAAATCAATTGAAGCCTCTC
TCCAAGCCATCAAAAAGAGATGTTCAGTCGTCCGTGTCATTGCTCACACTCAAGTTCACA
AATTAAAATTAACTCAAAAGAAAGCACACGTCCTTGAAATCCAAGTCAATGGTGGTTCAA
TCGTCGAAAAAGTTAACTTTGCTGTTGCCAACTTTGAAAAGACCGTCAACGTCACTGGTG
TCTTTGCCGAAAATGAATTAATCGATGTTAT----------CTCAACCGGTAAGACTGAA
TTCGATTTAACTGAAAAGACTATCAATCCAATGGGTGGTTTNGCTCACTACGGTATGGTT
AAACATGAATTTTTAATGCTTAAAGGTATGTTATTTAATTTGNATAAATTATTATTATTT
TTTTTTTATTATTATGTTTTTCTTTTTTCCATAATATTGTTAGATTTCTCCTTTGCTTTT
TCATTTTTTTCACTTCCTATTATGTTTAAGTTCTTTCTGAAAATGCTATTTTTTTTTCCC
CCATTTATTGTTATTTTTTTTCTTTTTCTTTTTTTATTATTATTATTATCATTATTATTA
TATAATTATACGATTCAAATACTAATATATAATTTTCAATAAATAGGTTGTGTTGCTGGT
CCAAGAAAGAGAGCCTTAACCTTAAGAAAGAGTATCACCACTCAAACTGGTCGTGCTGCC
CTCGAGAAAATCACTCTCAAATTCATCGATACCTCCAGCAAGTTTGGTCACGGTCTTCAT
CACAACCGCTGCAAGATAAGACTAAATATTTCGGCGTCAAGAAATCAAGATCAACTAAGG
GCTTAACATTCAATAAATTGATTTCA
Length of connected seq. 1276
Full length Seq ID -
Full length Seq. -
Length of full length seq. -