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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO414
Library AF
(Link to library)
Clone ID AFO414
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16463-1
Original site URL
Representative seq. ID AFO414P
(Link to Original site)
Representative DNA sequence 
>AFO414 (AFO414Q) /CSM/AF/AFO4-A/AFO414Q.Seq.d/
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAGTAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAAXXXXXXXXXXGCCTTA
AAGTATCGTTTCAATCCATTCGATGTANCAAAGATTTGGTCACACAAAGACTATCCATTA
ATTCAAATTGGTCGTATGGTTTTGAATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAA
CAAGCTGCCTTCTCACCATCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATG
TTACAAGGTCGTCTCTTCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTAT
CAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGT
GATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCT
TTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTC
GCTGCTCGTCAACCATACAATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATAT
AGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCC
GGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAA
GATTTAGGTGCTCGTTTATGTAAGGGTCTTGGTATTGATGTTAAGATGTCATCAAATTCG
CTGCTAGATCAAACTGTAATCAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
iyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRE
RIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKGSSDSE
RDPRGFAVKFYTEEGNFDMVGSNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFL
GQTPESTHQVSILFSDRGTPKSYRHMHGFSSHTL---

---ALKYRFNPFDVXKIWSHKDYPLIQIGRMVLNRNPENYFAEVEQAAFSPSHMVPGIEP
SPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQRDGFMAVNGNGGKGPN
YQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLYRLMSEDAKSRFVSNL
VGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDVKMSSNSLLDQTVIN
Translated Amino Acid sequence (All Frames) 
Frame A:
iyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRE
RIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFTRFSTVGGEKGSSDSE
RDPRGFAVKFYTEEGNFDMVGSNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFL
GQTPESTHQVSILFSDRGTPKSYRHMHGFSSHTL---

---ALKYRFNPFDVXKIWSHKDYPLIQIGRMVLNRNPENYFAEVEQAAFSPSHMVPGIEP
SPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQRDGFMAVNGNGGKGPN
YQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLYRLMSEDAKSRFVSNL
VGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDVKMSSNSLLDQTVIN

Frame B:
yilfgknk*ink*icqlqf*ppvvvhqliti*tq*pqvlmvqf*fkisl*linllisivn
vsqnvlymlkvlvpmvisklpqvmyqngvklns*iklvrehqsspvslllvvkkvhpilk
eiqevslsnfilkkvtliwsvvilqfslfvihqnsqisfihknvihkqivkiqicfgif*
vkhqnqhikyqfyslievhqnhivicmvshhip*---

---p*sivsihsmxqrfghtktih*fklvvwf*iviqritllklnklpshhptwfqvlnh
hqircykvvssliqiliviv*v*tinksqsivhsp*rvvlkttnvmvswlsmvmvvkvqt
inqtlsvvqshiqnllninltslvsllvnhtiiqmttlfnqviyidsclkmpnlvssli*
svtcpvspskkfkfvpfqtftrltki*vlvyvrvlvlmlrchqirc*ikl*s

Frame C:
ifylgkink*inkyvsssfnhq*wftn**qfklndrrc*wsnfnsrfhfn**tcsfrs*t
yprtcctc*rcwcpwlfrsylk*ctkmv*s*ilk*sw*entnlhpflycww*krfirf*k
rskrfrcqily*rr*l*ygr**yssflys*sikiprfhsytkt*stnkl*rskyvlgffr
sntrintssinfil**rytkiissyawflitylk---

---lkvsfqsircxkdlvtqrlsinsnwsygfes*srellc*s*tsclltiphgsry*ti
tr*dvtrsslllsryssssfrcklstnpsqlsirrkgwc*klpt*wfhgcqw*ww*rskl
stklfrwsratsricst*i*rlwfrcsstiqssk*rlcstr*fi*thv*rcqisfrl*fs
rshvrchhqrnssscrfkllqg*qrfrcsfm*gswy*c*dvikfaarsncnq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO414 (AFO414Q) /CSM/AF/AFO4-A/AFO414Q.Seq.d/                       2514   0.0
CFA669 (CFA669Q) /CSM/CF/CFA6-C/CFA669Q.Seq.d/                       1356   0.0
AFI691 (AFI691Q) /CSM/AF/AFI6-D/AFI691Q.Seq.d/                       1346   0.0
AFE708 (AFE708Q) /CSM/AF/AFE7-A/AFE708Q.Seq.d/                       1346   0.0
CFI634 (CFI634Q) /CSM/CF/CFI6-B/CFI634Q.Seq.d/                       1344   0.0
AFN135 (AFN135Q) /CSM/AF/AFN1-B/AFN135Q.Seq.d/                       1338   0.0
AFE574 (AFE574Q) /CSM/AF/AFE5-D/AFE574Q.Seq.d/                       1338   0.0
AFE230 (AFE230Q) /CSM/AF/AFE2-B/AFE230Q.Seq.d/                       1338   0.0
CFI568 (CFI568Q) /CSM/CF/CFI5-C/CFI568Q.Seq.d/                       1336   0.0
AFE294 (AFE294Q) /CSM/AF/AFE2-D/AFE294Q.Seq.d/                       1336   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF090443|AF090443.1 Dictyostelium discoideum catalase (cat) mRNA, complete cds.  1283  0.0  3
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1283  0.0  5
AB064338|AB064338.1 Candida boidinii CTA1 gene for peroxisomal catalase, complete cds.   111  5e-65  7
E65314|E65314.1 Novel catalase, gene of said catalase and composition containing  said  catalase, and process for preparing catalase using the genetic engineering  technology.    80  2e-31  4
AP004595|AP004595.1 Oceanobacillus iheyensis HTE831 genomic DNA, section 3/13.    72  4e-31  6
AX536932|AX536932.1 Sequence 533 from Patent WO02064766.         101  3e-29  3
AB006327|AB006327.1 Candida albicans mRNA for catalase, complete cds.    94  8e-27  3
U40704|U40704.1 Candida albicans catalase (Cat) gene, complete cds.    86  1e-26  3
AJ400965|AJ400965.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, strain PR.    76  3e-25  3
AJ400964|AJ400964.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, wild type.    76  3e-25  3
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF090443_1(AF090443|pid:none) Dictyostelium discoideum catalase ...   627   e-178
(P26901) RecName: Full=Vegetative catalase;          EC=1.11.1.6...   485   e-135
(O93662) RecName: Full=Catalase;          EC=1.11.1.6;  &AJ00593...   426   e-118
BC090377_1(BC090377|pid:none) Xenopus tropicalis hypothetical LO...   417   e-115
CP000300_481(CP000300|pid:none) Methanococcoides burtonii DSM 62...   415   e-114
AY242059_1(AY242059|pid:none) Melopsittacus undulatus catalase (...   410   e-113
CP001280_2409(CP001280|pid:none) Methylocella silvestris BL2, co...   407   e-112
(Q9PWF7) RecName: Full=Catalase;          EC=1.11.1.6;  &AB03187...   407   e-112
BC086479_1(BC086479|pid:none) Xenopus laevis hypothetical LOC495...   407   e-112
BC099349_1(BC099349|pid:none) Xenopus laevis hypothetical LOC495...   407   e-112
protein update 2009. 4.26
PSORT 

 psg: 0.89  gvh: 0.57  alm: 0.44  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 20.0 %: extracellular, including cell wall
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFO414 is cyt
5' end seq. ID AFO414F
5' end seq. 
>AFO414F.Seq
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAGTAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAA----------
Length of 5' end seq. 644
3' end seq. ID AFO414Z
3' end seq. 
>AFO414Z.Seq
----------GCCTTAAAGTATCGTTTCAATCCATTCGATGTANCAAAGATTTGGTCACA
CAAAGACTATCCATTAATTCAAATTGGTCGTATGGTTTTGAATCGTAATCCAGAGAATTA
CTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCATCCCACATGGTTCCAGGTATTGAACC
ATCACCAGATAAGATGTTACAAGGTCGTCTCTTCTCTTATCCAGATACTCATCGTCATCG
TTTAGGTGTAAACTATCAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAGGGTGGTGT
TAAAAACTACCAACGTGATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAAGGTCCAAA
CTATCAACCAAACTCTTTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAACATAAATT
TGACGTCTCTGGTTTCGCTGCTCGTCAACCATACAATCATCCAAATGACGACTTTGTTCA
ACCAGGTGATTTATATAGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTCTCTAATTT
AGTCGGTCACATGTCCGGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTTTCAAACTT
TTACAAGGCTGACAAAGATTTAGGTGCTCGTTTATGTAAGGGTCTTGGTATTGATGTTAA
GATGTCATCAAATTCGCTGCTAGATCAAACTGTAATCAAT
Length of 3' end seq. 690
Connected seq. ID AFO414P
Connected seq. 
>AFO414P.Seq
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCACCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAGTAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAA----------GCCTTA
AAGTATCGTTTCAATCCATTCGATGTANCAAAGATTTGGTCACACAAAGACTATCCATTA
ATTCAAATTGGTCGTATGGTTTTGAATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAA
CAAGCTGCCTTCTCACCATCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATG
TTACAAGGTCGTCTCTTCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTAT
CAACAAATCCCAGTCAATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGT
GATGGTTTCATGGCTGTCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCT
TTCGGTGGTCCAGAGCCACATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTC
GCTGCTCGTCAACCATACAATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATAT
AGACTCATGTCTGAAGATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCC
GGTGTCACCATCAAAGAAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAA
GATTTAGGTGCTCGTTTATGTAAGGGTCTTGGTATTGATGTTAAGATGTCATCAAATTCG
CTGCTAGATCAAACTGTAATCAAT
Length of connected seq. 1334
Full length Seq ID -
Full length Seq. -
Length of full length seq. -