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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK477
Library VH
(Link to library)
Clone ID VHK477
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11536-1
Original site URL
Representative seq. ID VHK477P
(Link to Original site)
Representative DNA sequence 
>VHK477 (VHK477Q) /CSM/VH/VHK4-D/VHK477Q.Seq.d/
GTTTTTGAATTTCCACCAAGAGTTTCAAATTTAACTTTATTATCTAGTAATAGCGTTACA
TTAAACTCTAATCAAACTATTGGATGGGAAATTGTAATTGATAGTGGTGGTGGTATTAAA
ATTAGTTATGTATTTATACATGTAATTTCAAATTTAGATAAAAAAGGATACACATTTGAA
TTCTATCCAAACTCTACCTCATGGGAAACCACAATTGGAATTGCTCTATCACCAGAAACA
ACACCATCACAAATATTTAAAATTTATAATGCAACTATTATTGACGAAAGAGGAATTAGA
TCGGACCATATTGATAGTTTTAAAGTTGATGAAATTTTTTCAAATCAAAGTCCAATTTAT
CCAATTTCAATTTCACCATTTTATTTATTTGATCTTAGTTTATTTAGCATAACAGTTAAT
TCACCATTCATTACTGAATCTGTTCCACCAACAATTACACTTGTAACCCTACCATTTGAA
AATGTAGATGATTTTTTACAATATTCAATTTTCTTTCAATTAGTGTCAACTGATAATGTT
GGTATATTAAAAAGCCATATACCACAAGTTTATTTATCGCATTCATCAAACTATGGAGAT
TTACAAATTTTCCAATGTAATACAGCTATTAATGATACGTCTGAAACAACTTGTAATTAT
TATGTTACTTGTAAAXXXXXXXXXXCACCAATTTTAAAAAACAATCAACATGTTTCAAAT
ATATCAGTAGCAATGGAATGGCATGACCAATCTACAACTATTACATTTGCAAAACAAAAT
CTCACAATTAATCCATCAACTTTGAAATATTATATTTCATTAGATAAATACCCATTTGCA
TCATCATTAAATACACTTGATTTAATCATTAAATCAATGTTTAAAAGTTCACAAACTGAT
GATGTATGTGGCAGTAAAGGATTTTCATTAAATGATGGAAATGATTATATGACATTACAA
ATTGGAACATACTCATTTGAAGGTAAATTTATAAAAAAAGCAATTATCAATGGCAATAGA
ATTGTAACAATAAATAATAAATTATTAAATGAAAATCAACTAAATAGTAATTCAACTATA
ACTAAAGAAAAAGATTCATCAAATCAACTTTCATCCTTTATCTCAATTGAAATACCTCAT
TATGATTCAAATGTATTATTAGATCCAAGTTTTTCAGTTTTATTAGATTCAAATGAATCA
TATAAAAACTCTGAAAATTCAATTTGTACTTCAAAAAAATCCTCAAAACTTACTACCGCT
CAAATAATTGCTATTGTTATTGTTTCTGTAGTTTTTGTAATAATTCTAGCTCTCTTTATA
ATCGTTAAATTTTCAAAATCTGATAAATTTTTATCTATAAGAATTTTCTATTATAAATTA
TTTAAACGATTTAAGAAAAAGTATTAAATTTATTTTTTAAAAAATAATATTAATTGTAAA
ATAAAGTTTAAAAAATAGTTTTTATTAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
VFEFPPRVSNLTLLSSNSVTLNSNQTIGWEIVIDSGGGIKISYVFIHVISNLDKKGYTFE
FYPNSTSWETTIGIALSPETTPSQIFKIYNATIIDERGIRSDHIDSFKVDEIFSNQSPIY
PISISPFYLFDLSLFSITVNSPFITESVPPTITLVTLPFENVDDFLQYSIFFQLVSTDNV
GILKSHIPQVYLSHSSNYGDLQIFQCNTAINDTSETTCNYYVTCK---

---PILKNNQHVSNISVAMEWHDQSTTITFAKQNLTINPSTLKYYISLDKYPFASSLNTL
DLIIKSMFKSSQTDDVCGSKGFSLNDGNDYMTLQIGTYSFEGKFIKKAIINGNRIVTINN
KLLNENQLNSNSTITKEKDSSNQLSSFISIEIPHYDSNVLLDPSFSVLLDSNESYKNSEN
SICTSKKSSKLTTAQIIAIVIVSVVFVIILALFIIVKFSKSDKFLSIRIFYYKLFKRFKK
KY*iyflknninckikfkk*fll
Translated Amino Acid sequence (All Frames) 
Frame A:
VFEFPPRVSNLTLLSSNSVTLNSNQTIGWEIVIDSGGGIKISYVFIHVISNLDKKGYTFE
FYPNSTSWETTIGIALSPETTPSQIFKIYNATIIDERGIRSDHIDSFKVDEIFSNQSPIY
PISISPFYLFDLSLFSITVNSPFITESVPPTITLVTLPFENVDDFLQYSIFFQLVSTDNV
GILKSHIPQVYLSHSSNYGDLQIFQCNTAINDTSETTCNYYVTCK---

---hqf*ktinmfqiyq*qwngmtnlqllhlqnkisqlihql*niifh*inthlhhh*ih
li*slnqclkvhklmmyvavkdfh*mmemii*hyklehthlkvnl*kkqlsmaiel*q*i
iny*mkin*iviql*lkkkihqinfhplsqlkylimiqmyy*iqvfqfy*iqmnhiktlk
iqfvlqknpqnllplk*lllllfl*fl**f*lsl*slnfqnlinfyl*efsiinylndlr
ksikfif*kiilivk*slknsfy*

Frame B:
flnfhqefqi*lyylvialh*tliklldgkl*livvvvlklvmylym*fqi*ikkdthln
siqtlphgkpqlellyhqkqhhhkylkfimqllltkeeldrtilivlklmkffqikvqfi
qfqfhhfiylilvyla*qlihhsllnlfhqqlhl*pyhlkm*mifyniqfsfn*cqliml
vy*kaiyhkfiyrihqtmeiykfsnviqllmirlkqlviimllv---

---tnfkkqstcfkyissngma*piynyyicktkshn*sinfeilyfir*ipiciiikyt
*fnh*inv*kftn**cmwq*rifik*wk*lyditnwnili*r*iykksnyqwq*ncnnk*
*iik*kstk**fnyn*rkrfikstfilyln*ntsl*fkciirskffsfirfk*ii*kl*k
fnlyfkkilktyyrsnncycycfcsfcnnssslynr*ifki**ifiyknfll*ii*ti*e
kvlnlffkk*y*l*nkv*kivfik

Frame C:
f*istksfkfnfii***ryikl*snywmgncn**wwwy*n*lciytcnfkfr*krihi*i
lsklylmgnhnwncsitrnntitni*nl*cnyy*rkrn*igpy**f*s**nffksksnls
nfnftilfi*s*fi*hns*ftihy*icstnnytcnpti*kcr*fftifnflsisvn**cw
yikkpyttslfiafiklwrftnfpm*ysy**yv*nnl*llcyl*---

---PILKNNQHVSNISVAMEWHDQSTTITFAKQNLTINPSTLKYYISLDKYPFASSLNTL
DLIIKSMFKSSQTDDVCGSKGFSLNDGNDYMTLQIGTYSFEGKFIKKAIINGNRIVTINN
KLLNENQLNSNSTITKEKDSSNQLSSFISIEIPHYDSNVLLDPSFSVLLDSNESYKNSEN
SICTSKKSSKLTTAQIIAIVIVSVVFVIILALFIIVKFSKSDKFLSIRIFYYKLFKRFKK
KY*iyflknninckikfkk*fll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK477 (VHK477Q) /CSM/VH/VHK4-D/VHK477Q.Seq.d/                       2569   0.0
VHG524 (VHG524Q) /CSM/VH/VHG5-A/VHG524Q.Seq.d/                       1273   0.0
AHQ777 (AHQ777Q) /CSM/AH/AHQ7-D/AHQ777Q.Seq.d/                       1249   0.0
VHK754 (VHK754Q) /CSM/VH/VHK7-C/VHK754Q.Seq.d/                       1237   0.0
VHI711 (VHI711Q) /CSM/VH/VHI7-A/VHI711Q.Seq.d/                       1195   0.0
VHE894 (VHE894Q) /CSM/VH/VHE8-D/VHE894Q.Seq.d/                       1174   0.0
VHG624 (VHG624Q) /CSM/VH/VHG6-A/VHG624Q.Seq.d/                        708   0.0
AFJ808 (AFJ808Q) /CSM/AF/AFJ8-A/AFJ808Q.Seq.d/                         46   0.002
VSH473 (VSH473Q) /CSM/VS/VSH4-D/VSH473Q.Seq.d/                         40   0.094
VSB889 (VSB889Q) /CSM/VS/VSB8-D/VSB889Q.Seq.d/                         40   0.094
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ424254) Dictyostelium discoideum cDNA clone:ddv51j20, 5' ...   658   0.0    2
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...   654   0.0    6
(BJ421902) Dictyostelium discoideum cDNA clone:ddv44o05, 5' ...   654   0.0    2
(BJ443088) Dictyostelium discoideum cDNA clone:ddv51j20, 3' ...   609   0.0    4
(BJ339305) Dictyostelium discoideum cDNA clone:dda64j19, 5' ...   569   0.0    2
(BJ443347) Dictyostelium discoideum cDNA clone:ddv52l13, 3' ...   525   0.0    5
(BJ420935) Dictyostelium discoideum cDNA clone:ddv40l24, 5' ...   654   0.0    2
(BJ441847) Dictyostelium discoideum cDNA clone:ddv48f03, 3' ...   422   0.0    5
(BJ357837) Dictyostelium discoideum cDNA clone:dda64j19, 3' ...   418   0.0    4
(BJ440606) Dictyostelium discoideum cDNA clone:ddv44o05, 3' ...   307   0.0    4
dna update 2008.11.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116957_52(AC116957|pid:none) Dictyostelium discoideum chromoso...   417   e-115
AC116957_18(AC116957|pid:none) Dictyostelium discoideum chromoso...   152   3e-35
AC116984_63(AC116984|pid:none) Dictyostelium discoideum chromoso...   146   2e-33
AC116984_70(AC116984|pid:none) Dictyostelium discoideum chromoso...   144   6e-33
AC115680_4(AC115680|pid:none) Dictyostelium discoideum chromosom...   138   6e-31
AC117072_39(AC117072|pid:none) Dictyostelium discoideum chromoso...   135   3e-30
AC116924_8(AC116924|pid:none) Dictyostelium discoideum chromosom...   135   4e-30
AC116984_134(AC116984|pid:none) Dictyostelium discoideum chromos...   133   2e-29
AC116100_9(AC116100|pid:none) Dictyostelium discoideum chromosom...   127   1e-27
AC115612_9(AC115612|pid:none) Dictyostelium discoideum chromosom...   125   3e-27
protein update 2009. 7.15
PSORT 

 psg: 0.84  gvh: 0.50  alm: 0.03  top: 0.53  tms: 0.07  mit: 0.36  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.05  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	 16.0 %: vesicles of secretory system
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: plasma membrane
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: vacuolar

>> prediction for VHK477 is nuc
5' end seq. ID VHK477F
5' end seq. 
>VHK477F.Seq
GTTTTTGAATTTCCACCAAGAGTTTCAAATTTAACTTTATTATCTAGTAATAGCGTTACA
TTAAACTCTAATCAAACTATTGGATGGGAAATTGTAATTGATAGTGGTGGTGGTATTAAA
ATTAGTTATGTATTTATACATGTAATTTCAAATTTAGATAAAAAAGGATACACATTTGAA
TTCTATCCAAACTCTACCTCATGGGAAACCACAATTGGAATTGCTCTATCACCAGAAACA
ACACCATCACAAATATTTAAAATTTATAATGCAACTATTATTGACGAAAGAGGAATTAGA
TCGGACCATATTGATAGTTTTAAAGTTGATGAAATTTTTTCAAATCAAAGTCCAATTTAT
CCAATTTCAATTTCACCATTTTATTTATTTGATCTTAGTTTATTTAGCATAACAGTTAAT
TCACCATTCATTACTGAATCTGTTCCACCAACAATTACACTTGTAACCCTACCATTTGAA
AATGTAGATGATTTTTTACAATATTCAATTTTCTTTCAATTAGTGTCAACTGATAATGTT
GGTATATTAAAAAGCCATATACCACAAGTTTATTTATCGCATTCATCAAACTATGGAGAT
TTACAAATTTTCCAATGTAATACAGCTATTAATGATACGTCTGAAACAACTTGTAATTAT
TATGTTACTTGTAAANNNNNNNNNN
Length of 5' end seq. 685
3' end seq. ID VHK477Z
3' end seq. 
>VHK477Z.Seq
NNNNNNNNNNCACCAATTTTAAAAAACAATCAACATGTTTCAAATATATCAGTAGCAATG
GAATGGCATGACCAATCTACAACTATTACATTTGCAAAACAAAATCTCACAATTAATCCA
TCAACTTTGAAATATTATATTTCATTAGATAAATACCCATTTGCATCATCATTAAATACA
CTTGATTTAATCATTAAATCAATGTTTAAAAGTTCACAAACTGATGATGTATGTGGCAGT
AAAGGATTTTCATTAAATGATGGAAATGATTATATGACATTACAAATTGGAACATACTCA
TTTGAAGGTAAATTTATAAAAAAAGCAATTATCAATGGCAATAGAATTGTAACAATAAAT
AATAAATTATTAAATGAAAATCAACTAAATAGTAATTCAACTATAACTAAAGAAAAAGAT
TCATCAAATCAACTTTCATCCTTTATCTCAATTGAAATACCTCATTATGATTCAAATGTA
TTATTAGATCCAAGTTTTTCAGTTTTATTAGATTCAAATGAATCATATAAAAACTCTGAA
AATTCAATTTGTACTTCAAAAAAATCCTCAAAACTTACTACCGCTCAAATAATTGCTATT
GTTATTGTTTCTGTAGTTTTTGTAATAATTCTAGCTCTCTTTATAATCGTTAAATTTTCA
AAATCTGATAAATTTTTATCTATAAGAATTTTCTATTATAAATTATTTAAACGATTTAAG
AAAAAGTATTAAATTTATTTTTTAAAAAATAATATTAATTGTAAAATAAAGTTTAAAAAA
TAGTTTTTATTAAA
Length of 3' end seq. 794
Connected seq. ID VHK477P
Connected seq. 
>VHK477P.Seq
GTTTTTGAATTTCCACCAAGAGTTTCAAATTTAACTTTATTATCTAGTAATAGCGTTACA
TTAAACTCTAATCAAACTATTGGATGGGAAATTGTAATTGATAGTGGTGGTGGTATTAAA
ATTAGTTATGTATTTATACATGTAATTTCAAATTTAGATAAAAAAGGATACACATTTGAA
TTCTATCCAAACTCTACCTCATGGGAAACCACAATTGGAATTGCTCTATCACCAGAAACA
ACACCATCACAAATATTTAAAATTTATAATGCAACTATTATTGACGAAAGAGGAATTAGA
TCGGACCATATTGATAGTTTTAAAGTTGATGAAATTTTTTCAAATCAAAGTCCAATTTAT
CCAATTTCAATTTCACCATTTTATTTATTTGATCTTAGTTTATTTAGCATAACAGTTAAT
TCACCATTCATTACTGAATCTGTTCCACCAACAATTACACTTGTAACCCTACCATTTGAA
AATGTAGATGATTTTTTACAATATTCAATTTTCTTTCAATTAGTGTCAACTGATAATGTT
GGTATATTAAAAAGCCATATACCACAAGTTTATTTATCGCATTCATCAAACTATGGAGAT
TTACAAATTTTCCAATGTAATACAGCTATTAATGATACGTCTGAAACAACTTGTAATTAT
TATGTTACTTGTAAA----------CACCAATTTTAAAAAACAATCAACATGTTTCAAAT
ATATCAGTAGCAATGGAATGGCATGACCAATCTACAACTATTACATTTGCAAAACAAAAT
CTCACAATTAATCCATCAACTTTGAAATATTATATTTCATTAGATAAATACCCATTTGCA
TCATCATTAAATACACTTGATTTAATCATTAAATCAATGTTTAAAAGTTCACAAACTGAT
GATGTATGTGGCAGTAAAGGATTTTCATTAAATGATGGAAATGATTATATGACATTACAA
ATTGGAACATACTCATTTGAAGGTAAATTTATAAAAAAAGCAATTATCAATGGCAATAGA
ATTGTAACAATAAATAATAAATTATTAAATGAAAATCAACTAAATAGTAATTCAACTATA
ACTAAAGAAAAAGATTCATCAAATCAACTTTCATCCTTTATCTCAATTGAAATACCTCAT
TATGATTCAAATGTATTATTAGATCCAAGTTTTTCAGTTTTATTAGATTCAAATGAATCA
TATAAAAACTCTGAAAATTCAATTTGTACTTCAAAAAAATCCTCAAAACTTACTACCGCT
CAAATAATTGCTATTGTTATTGTTTCTGTAGTTTTTGTAATAATTCTAGCTCTCTTTATA
ATCGTTAAATTTTCAAAATCTGATAAATTTTTATCTATAAGAATTTTCTATTATAAATTA
TTTAAACGATTTAAGAAAAAGTATTAAATTTATTTTTTAAAAAATAATATTAATTGTAAA
ATAAAGTTTAAAAAATAGTTTTTATTAAA
Length of connected seq. 1459
Full length Seq ID -
Full length Seq. -
Length of full length seq. -