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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO408
Library AF
(Link to library)
Clone ID AFO408
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15027-1
Original site URL
Representative seq. ID AFO408P
(Link to Original site)
Representative DNA sequence 
>AFO408 (AFO408Q) /CSM/AF/AFO4-A/AFO408Q.Seq.d/
GATTTTCATATTCCAACACAACCTATACTTCATCACATAATGTCCAAAATTGTTAAATTT
TTAAAAGAGTTAGCTACTCCATCTCACTCTATGGAATTTTTCCATAAACCAGCATCAAAC
TCTTTACTTGATGCATCAGAACTCAATTTTGTTAGAAGAAATATTAAAAGAGAGGATTTC
GGTCATGAAGTCTTAACAGGTGCTTTTGGTACATTAAAATCACCAGTTATTGTTGAATCA
ATTTTCCATAGCAGAATTGTTGCCTGTGAAGGTGGTGATGGTGAAGAACATGATATTCTC
TTCCATACTGTTGCTGAAAAGAAACCAACCATTTGTTTAGATTGTGGTCAAGTTTTCAAA
CTTAAACACATTAGCTCTGAAGGCGAAGTTATGTACTACTAAACTTAATCACATAATAAT
AATAATAATAACTAAAAAATACTATATAAAAAAAAAAXXXXXXXXXXGATTTTCATATTC
CAACACAACCTATACTTCATCACATAATGTCCAAAATTGTTAAATTTTTAAAAGAGTTAG
CTACTCCATCTCACTCTATGGAATTTTTCCATAAACCAGCATCAAACTCTTTACTTGATG
CATCAGAACTCAATTTTGTTAGAAGAAATATTAAAAGAGAGGATTTCGGTCATGAAGTCT
TAACAGGTGCTTTTGGTACATTAAAATCACCAGTTATTGTTGAATCAATTTTCCATAGCA
GAATTGTTGCCTGTGAAGGTGGTGATGGTGAAGAACATGATATTCTCTTCCATACTGTTG
CTGAAAAGAAACCAACCATTTGTTTAGATTGTGGTCAAGTTTTCAAACTTAAACACATTA
GCTCTGAAGGCGAAGTTATGTACTACTAAACTTAATCACATAATAATAATAATAAAACTA
AAAAAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ififqhnlyfit*cpkllnf*ks*llhltlwnfsinqhqtlylmhqnsilleeilkeris
vmks*qvllvh*nhqlllnqfsiaellpvkvvmvknmifssilllkrnqpfv*IVVKFSN
LNTLALKAKLCTTKLNHIIIIIITKKYYIKKK---

---DFHIPTQPILHHIMSKIVKFLKELATPSHSMEFFHKPASNSLLDASELNFVRRNIKR
EDFGHEVLTGAFGTLKSPVIVESIFHSRIVACEGGDGEEHDILFHTVAEKKPTICLDCGQ
VFKLKHISSEGEVMYY*t*shnnnnktkk
Translated Amino Acid sequence (All Frames) 
Frame A:
dfhiptqpilhhimskivkflkelatpshsmeffhkpasnslldaselnfvrrnikredf
ghevltgafgtlkspvivesifhsrivaceggdgeehdilfhtvaekkpticldcgqvfk
lkhissegevmyy*t*shnnnnnn*kilykkk---

---DFHIPTQPILHHIMSKIVKFLKELATPSHSMEFFHKPASNSLLDASELNFVRRNIKR
EDFGHEVLTGAFGTLKSPVIVESIFHSRIVACEGGDGEEHDILFHTVAEKKPTICLDCGQ
VFKLKHISSEGEVMYY*t*shnnnnktkk

Frame B:
ififqhnlyfit*cpkllnf*ks*llhltlwnfsinqhqtlylmhqnsilleeilkeris
vmks*qvllvh*nhqlllnqfsiaellpvkvvmvknmifssilllkrnqpfv*IVVKFSN
LNTLALKAKLCTTKLNHIIIIIITKKYYIKKK---

---ififqhnlyfit*cpkllnf*ks*llhltlwnfsinqhqtlylmhqnsilleeilke
risvmks*qvllvh*nhqlllnqfsiaellpvkvvmvknmifssilllkrnqpfv*ivvk
fsnlntlalkaklcttklnhiiiiiklkn

Frame C:
fsysnttytsshnvqnc*ifkrvsysislygifp*tsiklft*cirtqfc*kky*krgfr
s*slnrcfwyikitsyc*infp*qnccl*rw*w*rt*yslpycc*ketnhlfrlwssfqt
*th*l*rrsyvllnlit******lknti*kk---

---fsysnttytsshnvqnc*ifkrvsysislygifp*tsiklft*cirtqfc*kky*kr
gfrs*slnrcfwyikitsyc*infp*qnccl*rw*w*rt*yslpycc*ketnhlfrlwss
fqt*th*l*rrsyvllnlit*****n*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO408 (AFO408Q) /CSM/AF/AFO4-A/AFO408Q.Seq.d/                        870   0.0
VFH695 (VFH695Q) /CSM/VF/VFH6-D/VFH695Q.Seq.d/                        854   0.0
VHP755 (VHP755Q) /CSM/VH/VHP7-C/VHP755Q.Seq.d/                        842   0.0
SSB736 (SSB736Q) /CSM/SS/SSB7-B/SSB736Q.Seq.d/                        823   0.0
FC-BB18 (FC-BB18Q) /CSM/FC/FC-BB/FC-BB18Q.Seq.d/                      821   0.0
VSI447 (VSI447Q) /CSM/VS/VSI4-B/VSI447Q.Seq.d/                        813   0.0
VSE550 (VSE550Q) /CSM/VS/VSE5-C/VSE550Q.Seq.d/                        803   0.0
SSE627 (SSE627Q) /CSM/SS/SSE6-B/SSE627Q.Seq.d/                        803   0.0
SSB416 (SSB416Q) /CSM/SS/SSB4-A/SSB416Q.Seq.d/                        797   0.0
FC-AQ09 (FC-AQ09Q) /CSM/FC/FC-AQ/FC-AQ09Q.Seq.d/                      797   0.0
own update 2002.10.30
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X55671|X55671.1 Dictyostelium discoideum mRNA for cytochrome c oxidase subunit V (EC 1.9.3.1).   829  0.0  2
X64771|X64771.1 D.discoideum coxV gene for cytochrome c oxidase subunit V.   270  3e-68  1
AC107925|AC107925.5 Homo sapiens chromosome 11, clone RP11-379A20, complete sequence.    42  2e-05  4
AC090639|AC090639.6 Homo sapiens chromosome 11, clone RP11-1114G3, complete sequence.    42  0.002  4
AC115682|AC115682.2 Dictyostelium discoideum chromosome 2 map 4101105-4158643 strain AX4, complete sequence.    42  0.006  2
AC126013|AC126013.9 Medicago truncatula clone mth2-32e10, complete sequence.    40  0.006  6
AC009868|AC009868.3 Homo sapiens chromosome 11 clone RP11-156E12 map 11, WORKING DRAFT SEQUENCE, 43 unordered pieces.    42  0.022  4
AC120183|AC120183.1 Mus musculus clone RP24-116K23, LOW-PASS SEQUENCE SAMPLING.    40  0.023  2
AC009649|AC009649.4 Homo sapiens chromosome 11 clone RP11-323E3 map 11, *** SEQUENCING IN PROGRESS ***, 55 unordered pieces.    42  0.026  4
BX005470|BX005470.6 Zebrafish DNA sequence from clone CH211-122K23 in linkage group 18.    50  0.049  1
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X64771_1(X64771|pid:none) D.discoideum coxV gene for cytochrome ...    67   1e-09
EU952585_1(EU952585|pid:none) Zea mays clone 1283826 cytochrome ...    62   2e-08
AK071423_1(AK071423|pid:none) Oryza sativa Japonica Group cDNA c...    59   3e-07
AB236124_1(AB236124|pid:none) Pisum sativum coxVb mRNA for cytoc...    56   1e-06
AB236123_1(AB236123|pid:none) Pisum sativum coxVb mRNA for cytoc...    56   1e-06
EU969370_1(EU969370|pid:none) Zea mays clone 329254 cytochrome c...    56   2e-06
AB236125_1(AB236125|pid:none) Pisum sativum coxVb gene for cytoc...    56   2e-06
AY086953_1(AY086953|pid:none) Arabidopsis thaliana clone 29901 m...    54   9e-06
EF147854_1(EF147854|pid:none) Populus trichocarpa clone WS0125_H...    53   1e-05
AB017071_5(AB017071|pid:none) Arabidopsis thaliana genomic DNA, ...    53   2e-05
protein update 2009. 4.26
PSORT 

 psg: 0.79  gvh: 0.38  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for AFO408 is cyt
5' end seq. ID AFO408F
5' end seq. 
>AFO408F.Seq
GATTTTCATATTCCAACACAACCTATACTTCATCACATAATGTCCAAAATTGTTAAATTT
TTAAAAGAGTTAGCTACTCCATCTCACTCTATGGAATTTTTCCATAAACCAGCATCAAAC
TCTTTACTTGATGCATCAGAACTCAATTTTGTTAGAAGAAATATTAAAAGAGAGGATTTC
GGTCATGAAGTCTTAACAGGTGCTTTTGGTACATTAAAATCACCAGTTATTGTTGAATCA
ATTTTCCATAGCAGAATTGTTGCCTGTGAAGGTGGTGATGGTGAAGAACATGATATTCTC
TTCCATACTGTTGCTGAAAAGAAACCAACCATTTGTTTAGATTGTGGTCAAGTTTTCAAA
CTTAAACACATTAGCTCTGAAGGCGAAGTTATGTACTACTAAACTTAATCACATAATAAT
AATAATAATAACTAAAAAATACTATATAAAAAAAAAA----------
Length of 5' end seq. 457
3' end seq. ID AFO408Z
3' end seq. 
>AFO408Z.Seq
----------GATTTTCATATTCCAACACAACCTATACTTCATCACATAATGTCCAAAAT
TGTTAAATTTTTAAAAGAGTTAGCTACTCCATCTCACTCTATGGAATTTTTCCATAAACC
AGCATCAAACTCTTTACTTGATGCATCAGAACTCAATTTTGTTAGAAGAAATATTAAAAG
AGAGGATTTCGGTCATGAAGTCTTAACAGGTGCTTTTGGTACATTAAAATCACCAGTTAT
TGTTGAATCAATTTTCCATAGCAGAATTGTTGCCTGTGAAGGTGGTGATGGTGAAGAACA
TGATATTCTCTTCCATACTGTTGCTGAAAAGAAACCAACCATTTGTTTAGATTGTGGTCA
AGTTTTCAAACTTAAACACATTAGCTCTGAAGGCGAAGTTATGTACTACTAAACTTAATC
ACATAATAATAATAATAAAACTAAAAAAT
Length of 3' end seq. 439
Connected seq. ID AFO408P
Connected seq. 
>AFO408P.Seq
GATTTTCATATTCCAACACAACCTATACTTCATCACATAATGTCCAAAATTGTTAAATTT
TTAAAAGAGTTAGCTACTCCATCTCACTCTATGGAATTTTTCCATAAACCAGCATCAAAC
TCTTTACTTGATGCATCAGAACTCAATTTTGTTAGAAGAAATATTAAAAGAGAGGATTTC
GGTCATGAAGTCTTAACAGGTGCTTTTGGTACATTAAAATCACCAGTTATTGTTGAATCA
ATTTTCCATAGCAGAATTGTTGCCTGTGAAGGTGGTGATGGTGAAGAACATGATATTCTC
TTCCATACTGTTGCTGAAAAGAAACCAACCATTTGTTTAGATTGTGGTCAAGTTTTCAAA
CTTAAACACATTAGCTCTGAAGGCGAAGTTATGTACTACTAAACTTAATCACATAATAAT
AATAATAATAACTAAAAAATACTATATAAAAAAAAAA----------GATTTTCATATTC
CAACACAACCTATACTTCATCACATAATGTCCAAAATTGTTAAATTTTTAAAAGAGTTAG
CTACTCCATCTCACTCTATGGAATTTTTCCATAAACCAGCATCAAACTCTTTACTTGATG
CATCAGAACTCAATTTTGTTAGAAGAAATATTAAAAGAGAGGATTTCGGTCATGAAGTCT
TAACAGGTGCTTTTGGTACATTAAAATCACCAGTTATTGTTGAATCAATTTTCCATAGCA
GAATTGTTGCCTGTGAAGGTGGTGATGGTGAAGAACATGATATTCTCTTCCATACTGTTG
CTGAAAAGAAACCAACCATTTGTTTAGATTGTGGTCAAGTTTTCAAACTTAAACACATTA
GCTCTGAAGGCGAAGTTATGTACTACTAAACTTAATCACATAATAATAATAATAAAACTA
AAAAAT
Length of connected seq. 896
Full length Seq ID -
Full length Seq. -
Length of full length seq. -