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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO401
Library AF
(Link to library)
Clone ID AFO401
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFO401E
(Link to Original site)
Representative DNA sequence 
>AFO401 (AFO401Q) /CSM/AF/AFO4-A/AFO401Q.Seq.d/
ATTTCATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACA
AATAAAAAAAAAAATAAAAAATGGATGGNGATGATGTTCAAGCTTTAGTTATTGATAATG
GTTCAGGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCAT
CAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCAT
ATGTCGGNGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAAC
ATGGTATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATG
AACTTCGNGTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAA
AAGCAAATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGT
ATGTTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTA
TGGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCA
CATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATC
TTAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATT
AAAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCA
TCATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAAT
GAACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCT
GGTATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGAT
TTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGT
ATGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCA
CC
sequence update 2001. 6. 9
Translated Amino Acid sequence 
isllnnskdfnyililnk*tnkkknkkwmxmmfkl*llimvqvcvklvllvmmlhvlfsh
qllvvqdtlvlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfim
nfxllqrniqfx*lklh*iqkqierk*LKSCLKLSTLQQCMLPFKLYYHSMPLVVPLVSL
WIQVMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDI
KEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESA
GIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAP
Translated Amino Acid sequence (All Frames) 
Frame A:
isllnnskdfnyililnk*tnkkknkkwmxmmfkl*llimvqvcvklvllvmmlhvlfsh
qllvvqdtlvlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfim
nfxllqrniqfx*lklh*iqkqierk*LKSCLKLSTLQQCMLPFKLYYHSMPLVVPLVSL
WIQVMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDI
KEKLAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESA
GIHETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAP


Frame B:
fhy*ttqkiliif*y*inkqikkkikngwx*cssfsy**wfryv*swfcw**cstccfpi
ncwsskthwcygwygskgficrx*ssikkryfnt*vsn*twychqlg*ygknlasyfl**
tsxcsrgtsssxn*ssiksksk*rendsnhv*nfqhssnvcchssciitlclwsyhwyry
gfr*wcfpyctnlrrvmhyhmlfyv*i*lvvillit**ks*lnvvthsqqllkeksleil
krn*htlhwilklkcklqphhlh*rnhmnyqmvkllqlvmnvsvvqkhfsnhhswvwsll
vfmkqpiiqs*nvmlifvkiymvmlyyqvvqlcsqvlliv*ikn*lhlhhqq*klkllhh


Frame C:
fiikqlkrf*lyfnik*ink*kkk*kmdgddvqalvidngsgmckagfagddapravfps
ivgrprhtgvmvgmgqkdsyvgdeaqskrgiltlkypiehgivtnwddmekiwhhtfyne
lrvapeehpvllteaplnpkanrekmtqimfetfntpamyvaiqavlslyasgrttgivm
dsgdgvshtvpiyeglcittcyftfrfsws*sy*lhdenln*twllihnnc*krnr*ry*
reisircigf*s*nancsliicireii*itrwssyynw**tfplsrstfptiilgygvcw
ys*nnl*fnhem*c*ys*rfiw*cciirwynyvpryc*sye*rinctctinnen*nyctt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO401 (AFO401Q) /CSM/AF/AFO4-A/AFO401Q.Seq.d/                       1961   0.0
SFL261 (SFL261Q) /CSM/SF/SFL2-C/SFL261Q.Seq.d/                       1953   0.0
SFD319 (SFD319Q) /CSM/SF/SFD3-A/SFD319Q.Seq.d/                       1953   0.0
CHD357 (CHD357Q) /CSM/CH/CHD3-C/CHD357Q.Seq.d/                       1953   0.0
CFI221 (CFI221Q) /CSM/CF/CFI2-A/CFI221Q.Seq.d/                       1953   0.0
AFO158 (AFO158Q) /CSM/AF/AFO1-C/AFO158Q.Seq.d/                       1953   0.0
AFN507 (AFN507Q) /CSM/AF/AFN5-A/AFN507Q.Seq.d/                       1953   0.0
AFE346 (AFE346Q) /CSM/AF/AFE3-B/AFE346Q.Seq.d/                       1953   0.0
AFA723 (AFA723Q) /CSM/AF/AFA7-A/AFA723Q.Seq.d/                       1953   0.0
AFA157 (AFA157Q) /CSM/AF/AFA1-C/AFA157Q.Seq.d/                       1953   0.0
own update 2003. 5.17
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   811  0.0  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   779  0.0  6
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   731  0.0  6
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   731  0.0  13
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     700  0.0  2
X03284|X03284.1 Dictyostelium discoideum gene for actin A3-S2.   692  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   668  0.0  7
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        613  0.0  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   613  0.0  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   597  0.0  5
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   348   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   348   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        342   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   346   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   347   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   340   0.0
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   343   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          339   0.0
AY294153_1(AY294153|pid:none) Platyamoeba placida actin mRNA, pa...   339   e-180
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   342   e-180
protein update 2009. 6.29
PSORT 

 psg: 0.82  gvh: 0.45  alm: 0.26  top: 0.53  tms: 0.07  mit: 0.41  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.38  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFO401 is mit
5' end seq. ID AFO401F
5' end seq. 
>AFO401F.Seq
ATTTCATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACA
AATAAAAAAAAAAATAAAAAATGGATGGNGATGATGTTCAAGCTTTAGTTATTGATAATG
GTTCAGGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCAT
CAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCAT
ATGTCGGNGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAAC
ATGGTATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATG
AACTTCGNGTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAA
AAGCAAATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGT
ATGTTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTA
TGGATTCAGG----------
Length of 5' end seq. 550
3' end seq. ID AFO401Z
3' end seq. 
>AFO401Z.Seq
----------AGCAATGTATGTTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTC
GTACCACTGGTATCGTTATGGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACG
AAGGGTTATGCATTACCACATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACT
GATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGA
GAAATCGTTAGAGATATTAAAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAA
ATGCAAACTGCAGCCTCATCATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAA
GTTATTACAATTGGTAATGAACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTC
TTGGGTATGGAGTCTGCTGGTATTCATGAAACAACCTATAATTCAATCATGAAATGTGAT
GTTGATATTCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTC
CCAGGTATTGCTGATCGTATGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAA
ATTAAAATTATTGCACCACC
Length of 3' end seq. 610
Connected seq. ID AFO401P
Connected seq. 
>AFO401P.Seq
ATTTCATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACA
AATAAAAAAAAAAATAAAAAATGGATGGNGATGATGTTCAAGCTTTAGTTATTGATAATG
GTTCAGGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCAT
CAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCAT
ATGTCGGNGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAAC
ATGGTATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATG
AACTTCGNGTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAA
AAGCAAATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGT
ATGTTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTA
TGGATTCAGG----------AGCAATGTATGTTGCCATTCAAGCTGTATTATCACTCTAT
GCCTCTGGTCGTACCACTGGTATCGTTATGGATTCAGGTGATGGTGTTTCCCATACTGTA
CCAATTTACGAAGGGTTATGCATTACCACATGCTATTTTACGTTTAGATTTAGCTGGTCG
TGATCTTACTGATTACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACAACAAC
TGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGAAATTAGCATACGTTGCATTGGATTT
TGAAGCTGAAATGCAAACTGCAGCCTCATCATCTGCATTAGAGAAATCATATGAATTACC
AGATGGTCAAGTTATTACAATTGGTAATGAACGTTTCCGTTGTCCAGAAGCACTTTTCCA
ACCATCATTCTTGGGTATGGAGTCTGCTGGTATTCATGAAACAACCTATAATTCAATCAT
GAAATGTGATGTTGATATTCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTAC
AACTATGTTCCCAGGTATTGCTGATCGTATGAATAAAGAATTAACTGCACTTGCACCATC
AACAATGAAAATTAAAATTATTGCACCACC
Length of connected seq. 1160
Full length Seq ID AFO401E
Full length Seq. 
>AFO401E.Seq
ATTTCATTATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACA
AATAAAAAAAAAAATAAAAAATGGATGGNGATGATGTTCAAGCTTTAGTTATTGATAATG
GTTCAGGTATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCAT
CAATTGTTGGTCGTCCAAGACACACTGGTGTTATGGTTGGTATGGGTCAAAAGGATTCAT
ATGTCGGNGATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAAC
ATGGTATTGTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATG
AACTTCGNGTTGCTCCAGAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAA
AAGCAAATAGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGT
ATGTTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTA
TGGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCA
CATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATC
TTAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATT
AAAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCA
TCATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAAT
GAACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCT
GGTATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGAT
TTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGT
ATGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCA
CC
Length of full length seq. 1082