>AFO388 (AFO388Q) /CSM/AF/AFO3-D/AFO388Q.Seq.d/
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGGXXXXXXXXXXA
TCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACC
CAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAG
CTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCG
CCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAG
GTGAATGGGNACNCCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACC
TCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCT
TTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTNTCTGGAAAGACCT
TTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTG
AAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCG
AAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGNCA
AAAAAGAAGAAGTCAAAAAATCAACCAAAAAAAGTT

nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTL---

---IKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEA
PTIANPEYKGEWKAKKIPNPEYKGEWXXPLIDNPEYAEDNELYLFNDLGAIGFELWQVKS
GSIFNNMIVXDSVEEAKDXSGKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAE
KEAEEADEEEEEVAEEDLVKTDXKKEEVKKSTKKS

Frame A:
nfsnhhiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVS
DWHKEDGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVK
NEQKVDCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKK
EINKVETDQLTHQYTL---

---IKDPKQSKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEA
PTIANPEYKGEWKAKKIPNPEYKGEWXXPLIDNPEYAEDNELYLFNDLGAIGFELWQVKS
GSIFNNMIVXDSVEEAKDXSGKTFVANQEAEKKMFDDLEAAKAEERKKADEKLAAEKAAE
KEAEEADEEEEEVAEEDLVKTDXKKEEVKKSTKKS

Frame B:
ispiiifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllv
igikkmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*r
mnkklivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srk
kstklkpin*lintlw---

---skiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkp
qpspiqntkvngkqrksqiqntkvngxxh*litqntlktmnstslmisvlsvsnygklnl
vpslti*ssptlskklkixlerpllptkkpkrkclttskppklkrerkltkn*lpkkllk
kkpkklmkkkkkslkki*lklmxkkkksknqpkkv

Frame C:
flqssyl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**
la*rrw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke
*tks*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqer
nqqs*nrsinssihf---

---qrsktiqtsrlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgsp
nhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tlpl**srcyrfrimas*iw
fhl*qydrxrlcrrs*rfxwkdlccqprsrkenv*rprsrqs*rekes*rkiscrksc*k
rsrrs**rrrrsr*rrfs*n*xqkrrsqkinqkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO388 (AFO388Q) /CSM/AF/AFO3-D/AFO388Q.Seq.d/                       1848   0.0
VFN642 (VFN642Q) /CSM/VF/VFN6-B/VFN642Q.Seq.d/                        942   0.0
VFM435 (VFM435Q) /CSM/VF/VFM4-B/VFM435Q.Seq.d/                        942   0.0
VFM276 (VFM276Q) /CSM/VF/VFM2-D/VFM276Q.Seq.d/                        942   0.0
VFL360 (VFL360Q) /CSM/VF/VFL3-C/VFL360Q.Seq.d/                        942   0.0
VFL214 (VFL214Q) /CSM/VF/VFL2-A/VFL214Q.Seq.d/                        942   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        942   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        942   0.0
VFH220 (VFH220Q) /CSM/VF/VFH2-A/VFH220Q.Seq.d/                        942   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                        942   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   942  0.0  3
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  3e-28  4
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    72  2e-19  4
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  8e-11  2
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  5e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74  5e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74  5e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74  5e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  5e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  5e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   331   e-166
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   206   3e-96
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   201   4e-96
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   209   8e-96
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   204   8e-96
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   204   8e-96
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   204   1e-95
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   199   5e-95
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   197   2e-94
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   203   3e-94

 psg: 1.01  gvh: 0.69  alm: 0.47  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.06  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: extracellular, including cell wall
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: vacuolar
	  8.0 %: endoplasmic reticulum

>> prediction for AFO388 is exc

>AFO388F.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGG----------

>AFO388Z.Seq
----------ATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGA
AATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGT
TGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGC
CCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCC
AGAATACAAAGGTGAATGGGNACNCCCATTAATTGATAACCCAGAATACGCTGAAGACAA
TGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATC
TGGTTCCATCTTTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTNTC
TGGAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGC
CGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGA
AAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAA
AACTGATGNCAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAAGTT

>AFO388P.Seq
AATTTCTCCAATCATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAAT
ACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTA
TCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGT
GATTGGCATAAAGAAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTT
GGTGATGAAAATCAAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCA
GCTAAATTCCCAAGTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAG
AATGAACAAAAAGTTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGAT
CAATCAGCATTCGATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGT
GCCTCAAAGAGAGTTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAA
GAAATCAACAAAGTTGAAACCGATCAATTAACTCATCAATACACTTTGG----------A
TCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACC
CAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAG
CTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCG
CCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAG
GTGAATGGGNACNCCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACC
TCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCT
TTAACAATATGATCGTCNCCGACTCTGTCGAAGAAGCTAAAGATTTNTCTGGAAAGACCT
TTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTG
AAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCG
AAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGATGNCA
AAAAAGAAGAAGTCAAAAAATCAACCAAAAAAAGTT