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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK274
Library VH
(Link to library)
Clone ID VHK274
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U10981-1
Original site URL
Representative seq. ID VHK274P
(Link to Original site)
Representative DNA sequence 
>VHK274 (VHK274Q) /CSM/VH/VHK2-D/VHK274Q.Seq.d/
AGAAAAAAACCCAATAAATTTAAACATACGAATATAACATGTATTCGATTAATAGAATTG
GTATACATGGTATATCAAATAAAATACTAAGTAGGCCAAAATTTTTTCATTCAAGTTCAT
CAATATTCTCCCTAAACCAAAATTTAAATAATTTTAAAAATAATAATAATTATATTTTAA
ATAGTTACAATAAAATAACACATAATCATCATCATCAAACATTATTTCATAGACATTATT
CAAATTTAAAGCAAGAGCAAGAAAATAAACAACAAAATGTAGGGAAAAATAGCGAAACAT
CACCTTCATCAACAACAACAACAGAAAATATAACAACAGCAACAACAGCAACAACAAAAA
CAGATATTAAAGAAACTCAAAATTCTACAATGTCAATGTTAAAAACAGTATTTAAATATT
TATGGCCAAAAGATAATAATGATTCAAAGATTAGAATTATAACATCAGTATTATTATTAT
TAAGTGCCAAAGTGTTAACGGTTCAAATACCATTTATATTTAAAGATATAGTTGATAGTT
TAACGACTACACAATCAGAGATGTTAACATTACCATTAXXXXXXXXXXATTCAATGATAC
CNTTTACTATAACATTGCCTATGGTAATCCNAACGCTACAAAAGAACAAGTTGAAAATGC
TGNTCGTGCTGNTCNCATTCATGAAGTTATTTTAAATATGAAAAATGGTTATGATACAGT
TGTAGGTGAACGTGGTTTAAAATTATNTGGCGGTGAAAAACAAANAGTTTCAATTGNTCG
NGCAATTTTAAAGGATTNTCCNATCGTTTTTTATGATGAGGCTACCTNTTCATTGGATAC
TGAAAAANAGAAATTAATTATGGACGCTTTAANAGAATTATTTAAAGGTNGTACCNCTAT
TATGATAGCTCATCGTTTAAGTACNATNGTAGATGCTGATGAAATCATAGTTTTAGGTAC
TGGTGGTATTATTTTAGAACGTGGTAATCATCAACNATTATTAGAATTAGAAGGAAAATA
TAGATCAATGTGGTTGGCCCNACNATAAAAAAAAAAATAATTAAAAAAATAGATTAACTA
ATTTGGTTTTAAATGTTAAGATAAACTCAAATAAACTGTTTAAAATTTTTATTATGTCTT
TTTTATTTTTTTCATTTT
sequence update 2002.10.25
Translated Amino Acid sequence 
kktq*i*TYEYNMYSINRIGIHGISNKILSRPKFFHSSSSIFSLNQNLNNFKNNNNYILN
SYNKITHNHHHQTLFHRHYSNLKQEQENKQQNVGKNSETSPSSTTTTENITTATTATTKT
DIKETQNSTMSMLKTVFKYLWPKDNNDSKIRIITSVLLLLSAKVLTVQIPFIFKDIVDSL
TTTQSEMLTLPL---

---FNDTXYYNIAYGNPNATKEQVENAXRAXXIHEVILNMKNGYDTVVGERGLKLXGGEK
QXVSIXRAILKDXPIVFYDEATXSLDTEKXKLIMDALXELFKGXTXIMIAHRLSTXVDAD
EIIVLGTGGIILERGNHQXLLELEGKYRSMWLAXX*kkk*lkk*in*fgfkc*dklk*tv
*nfyyvffiffi
Translated Amino Acid sequence (All Frames) 
Frame A:
rkkpnkfkhtnitcirlielvymvyqiky*vgqnffiqvhqysp*tki*iilkiiiiif*
ivtik*hiiiiikhyfidiiqi*skskkinnkm*gkiakhhlhqqqqqki*qqqqqqqqk
qilkklkilqcqc*kqylniygqkiimiqrlel*hqyyyy*vpkc*rfkyhlylki*liv
*rlhnqrc*hyh---

---iq*yxll*hclw*sxrykrts*kcxscxshs*syfkyekwl*yscr*twfkixwr*k
txsfnxsxnfkgxsxrfl**gylfigy*kxeinygrfxrii*rxyxyydsssfkyxxrc*
*nhsfrywwyyfrtw*sstiirirrki*invvgptikkkiikkid*liwf*mlr*tqinc
lkfllclfyffhf

Frame B:
eknpinlniri*hvfd**nwytwyik*ntk*akifsfkfinilpkpkfk*f*k***lyfk
*lq*nnt*ssssniis*tlfkfkarark*ttkcrek*rnitfinnnnrkynnsnnsnnkn
ry*rnskfynvnvknsi*ifmakr***fkd*nynisiiiikcqsvngsntiyi*rys**f
ndytirdvniti---

---FNDTXYYNIAYGNPNATKEQVENAXRAXXIHEVILNMKNGYDTVVGERGLKLXGGEK
QXVSIXRAILKDXPIVFYDEATXSLDTEKXKLIMDALXELFKGXTXIMIAHRLSTXVDAD
EIIVLGTGGIILERGNHQXLLELEGKYRSMWLAXX*kkk*lkk*in*fgfkc*dklk*tv
*nfyyvffiffi

Frame C:
kktq*i*TYEYNMYSINRIGIHGISNKILSRPKFFHSSSSIFSLNQNLNNFKNNNNYILN
SYNKITHNHHHQTLFHRHYSNLKQEQENKQQNVGKNSETSPSSTTTTENITTATTATTKT
DIKETQNSTMSMLKTVFKYLWPKDNNDSKIRIITSVLLLLSAKVLTVQIPFIFKDIVDSL
TTTQSEMLTLPL---

---smipftitlpmvixtlqknklkmlxvlxxfmklf*i*kmvmiql*vnvv*nyxavkn
kxfqlxxqf*rixxsffmmrlpxhwilkxrn*lwtl*xnylkvvpll**liv*vxx*mlm
ks*f*vlvvlf*nvviinxy*n*kenidqcgwpxxkkknn*knrltnlvlnvkinsnklf
kifimsflffsf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK274 (VHK274Q) /CSM/VH/VHK2-D/VHK274Q.Seq.d/                       1172   0.0
VHH259 (VHH259Q) /CSM/VH/VHH2-C/VHH259Q.Seq.d/                        726   0.0
VHQ133 (VHQ133Q) /CSM/VH/VHQ1-B/VHQ133Q.Seq.d/                        712   0.0
VHL510 (VHL510Q) /CSM/VH/VHL5-A/VHL510Q.Seq.d/                        656   0.0
VHD846 (VHD846Q) /CSM/VH/VHD8-B/VHD846Q.Seq.d/                        620   e-176
VHH355 (VHH355Q) /CSM/VH/VHH3-C/VHH355Q.Seq.d/                        605   e-172
AFJ482 (AFJ482Q) /CSM/AF/AFJ4-D/AFJ482Q.Seq.d/                        581   e-165
VHL846 (VHL846Q) /CSM/VH/VHL8-B/VHL846Q.Seq.d/                        428   e-119
VHO167 (VHO167Q) /CSM/VH/VHO1-C/VHO167Q.Seq.d/                         40   0.074
VHD121 (VHD121Q) /CSM/VH/VHD1-A/VHD121Q.Seq.d/                         40   0.074
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AF466308) Dictyostelium discoideum ABC transporter AbcB5 (a...   755   0.0    5
(BJ443053) Dictyostelium discoideum cDNA clone:ddv51c20, 3' ...   783   0.0    2
(BJ424216) Dictyostelium discoideum cDNA clone:ddv51c20, 5' ...   389   0.0    3
(BJ425036) Dictyostelium discoideum cDNA clone:ddv54l12, 5' ...   389   0.0    3
(BJ422363) Dictyostelium discoideum cDNA clone:ddv45n13, 5' ...   389   0.0    3
(BJ422318) Dictyostelium discoideum cDNA clone:ddv45e16, 5' ...   389   0.0    3
(BJ424912) Dictyostelium discoideum cDNA clone:ddv54c03, 5' ...   389   0.0    3
(BJ427678) Dictyostelium discoideum cDNA clone:ddv63a09, 5' ...   389   0.0    3
(BJ327255) Dictyostelium discoideum cDNA clone:dda19d22, 5' ...   343   0.0    3
(BJ420156) Dictyostelium discoideum cDNA clone:ddv38l12, 5' ...   389   0.0    5
dna update 2008.11.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000577_1343(CP000577|pid:none) Rhodobacter sphaeroides ATCC 17...   138   4e-31
CP000143_1282(CP000143|pid:none) Rhodobacter sphaeroides 2.4.1 c...   137   6e-31
CP000661_1847(CP000661|pid:none) Rhodobacter sphaeroides ATCC 17...   137   7e-31
CP001154_1404(CP001154|pid:none) Laribacter hongkongensis HLHK9,...   133   1e-29
AE016825_2184(AE016825|pid:none) Chromobacterium violaceum ATCC ...   132   3e-29
CP001635_813(CP001635|pid:none) Variovorax paradoxus S110 chromo...   130   1e-28
CP000362_1657(CP000362|pid:none) Roseobacter denitrificans OCh 1...   129   2e-28
CP000087_1092(CP000087|pid:none) Rickettsia bellii RML369-C, com...   129   2e-28
CP000683_198(CP000683|pid:none) Rickettsia massiliae MTU5, compl...   128   3e-28
CP001503_2917(CP001503|pid:none) Burkholderia glumae BGR1 chromo...   128   4e-28
protein update 2009. 7.15
PSORT 

 psg: 0.80  gvh: 0.43  alm: 0.28  top: 0.20  tms: 0.07  mit: 0.41  mip: 0.12
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 20.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	 12.0 %: cytoplasmic
	 12.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHK274 is mit
5' end seq. ID VHK274F
5' end seq. 
>VHK274F.Seq
AGAAAAAAACCCAATAAATTTAAACATACGAATATAACATGTATTCGATTAATAGAATTG
GTATACATGGTATATCAAATAAAATACTAAGTAGGCCAAAATTTTTTCATTCAAGTTCAT
CAATATTCTCCCTAAACCAAAATTTAAATAATTTTAAAAATAATAATAATTATATTTTAA
ATAGTTACAATAAAATAACACATAATCATCATCATCAAACATTATTTCATAGACATTATT
CAAATTTAAAGCAAGAGCAAGAAAATAAACAACAAAATGTAGGGAAAAATAGCGAAACAT
CACCTTCATCAACAACAACAACAGAAAATATAACAACAGCAACAACAGCAACAACAAAAA
CAGATATTAAAGAAACTCAAAATTCTACAATGTCAATGTTAAAAACAGTATTTAAATATT
TATGGCCAAAAGATAATAATGATTCAAAGATTAGAATTATAACATCAGTATTATTATTAT
TAAGTGCCAAAGTGTTAACGGTTCAAATACCATTTATATTTAAAGATATAGTTGATAGTT
TAACGACTACACAATCAGAGATGTTAACATTACCATTANNNNNNNNNN
Length of 5' end seq. 588
3' end seq. ID VHK274Z
3' end seq. 
>VHK274Z.Seq
NNNNNNNNNNATTCAATGATACCNTTTACTATAACATTGCCTATGGTAATCCNAACGCTA
CAAAAGAACAAGTTGAAAATGCTGNTCGTGCTGNTCNCATTCATGAAGTTATTTTAAATA
TGAAAAATGGTTATGATACAGTTGTAGGTGAACGTGGTTTAAAATTATNTGGCGGTGAAA
AACAAANAGTTTCAATTGNTCGNGCAATTTTAAAGGATTNTCCNATCGTTTTTTATGATG
AGGCTACCTNTTCATTGGATACTGAAAAANAGAAATTAATTATGGACGCTTTAANAGAAT
TATTTAAAGGTNGTACCNCTATTATGATAGCTCATCGTTTAAGTACNATNGTAGATGCTG
ATGAAATCATAGTTTTAGGTACTGGTGGTATTATTTTAGAACGTGGTAATCATCAACNAT
TATTAGAATTAGAAGGAAAATATAGATCAATGTGGTTGGCCCNACNATAAAAAAAAAAAT
AATTAAAAAAATAGATTAACTAATTTGGTTTTAAATGTTAAGATAAACTCAAATAAACTG
TTTAAAATTTTTATTATGTCTTTTTTATTTTTTTCATTTT
Length of 3' end seq. 580
Connected seq. ID VHK274P
Connected seq. 
>VHK274P.Seq
AGAAAAAAACCCAATAAATTTAAACATACGAATATAACATGTATTCGATTAATAGAATTG
GTATACATGGTATATCAAATAAAATACTAAGTAGGCCAAAATTTTTTCATTCAAGTTCAT
CAATATTCTCCCTAAACCAAAATTTAAATAATTTTAAAAATAATAATAATTATATTTTAA
ATAGTTACAATAAAATAACACATAATCATCATCATCAAACATTATTTCATAGACATTATT
CAAATTTAAAGCAAGAGCAAGAAAATAAACAACAAAATGTAGGGAAAAATAGCGAAACAT
CACCTTCATCAACAACAACAACAGAAAATATAACAACAGCAACAACAGCAACAACAAAAA
CAGATATTAAAGAAACTCAAAATTCTACAATGTCAATGTTAAAAACAGTATTTAAATATT
TATGGCCAAAAGATAATAATGATTCAAAGATTAGAATTATAACATCAGTATTATTATTAT
TAAGTGCCAAAGTGTTAACGGTTCAAATACCATTTATATTTAAAGATATAGTTGATAGTT
TAACGACTACACAATCAGAGATGTTAACATTACCATTA----------ATTCAATGATAC
CNTTTACTATAACATTGCCTATGGTAATCCNAACGCTACAAAAGAACAAGTTGAAAATGC
TGNTCGTGCTGNTCNCATTCATGAAGTTATTTTAAATATGAAAAATGGTTATGATACAGT
TGTAGGTGAACGTGGTTTAAAATTATNTGGCGGTGAAAAACAAANAGTTTCAATTGNTCG
NGCAATTTTAAAGGATTNTCCNATCGTTTTTTATGATGAGGCTACCTNTTCATTGGATAC
TGAAAAANAGAAATTAATTATGGACGCTTTAANAGAATTATTTAAAGGTNGTACCNCTAT
TATGATAGCTCATCGTTTAAGTACNATNGTAGATGCTGATGAAATCATAGTTTTAGGTAC
TGGTGGTATTATTTTAGAACGTGGTAATCATCAACNATTATTAGAATTAGAAGGAAAATA
TAGATCAATGTGGTTGGCCCNACNATAAAAAAAAAAATAATTAAAAAAATAGATTAACTA
ATTTGGTTTTAAATGTTAAGATAAACTCAAATAAACTGTTTAAAATTTTTATTATGTCTT
TTTTATTTTTTTCATTTT
Length of connected seq. 1148
Full length Seq ID -
Full length Seq. -
Length of full length seq. -