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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK258
Library VH
(Link to library)
Clone ID VHK258
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10823-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHK258 (VHK258Q) /CSM/VH/VHK2-C/VHK258Q.Seq.d/
XXXXXXXXXXATANGTTACACCAGTTGATGGTATTNCAATGACCACTAAAATCTCTAGAA
CCTTNCCATTTGAATTGATTATNGCCNTGGATTNTTCATNACAAGCAANTGGTCAATTAT
TNNTGGATGATGGTGAATCNATTCAAACNTATGNTGATNATAAANANTNTTTCATCCAAT
TCGATGNTGTNTCCTCANCCATNTTCATTTGCATACAAATTACAATCAACCANTCTCAAT
AACAATTATAATGGTACCGNTTNTTTAATCATNAANTCTATCCAAATANACGGNTCGCCA
NCAGTTCANCAAGTCAGTGTTAAGTGGTAGCCCAATCAATTCATCTAATGCTGTTTCNGA
NTCAACTCTCT
sequence update 2002. 9.10
Translated Amino Acid sequence 
---xlhqlmvxq*plkslepxhln*LXPWIXHXKQXVNYXWMMVNXFKXMXXIXXXSSNS
MXXPXPXSFAYKLQSTXLNNNYNGTXXLIXXSIQIXGSPXVXQVSVKW*pnqfi*ccfxx
ns
Translated Amino Acid sequence (All Frames) 
Frame A:
---xvtpvdgixmttkisrtxpfelixaxdxsxqaxgqlxxddgesiqtyxdxkxxfiqf
dxvssxxficiqitinxsq*ql*wyrxfnhxxypnxrxaxssxsqc*vvaqsihlmlfxx
qls


Frame B:
---xlhqlmvxq*plkslepxhln*LXPWIXHXKQXVNYXWMMVNXFKXMXXIXXXSSNS
MXXPXPXSFAYKLQSTXLNNNYNGTXXLIXXSIQIXGSPXVXQVSVKW*pnqfi*ccfxx
ns


Frame C:
---xyts*wyxndh*nl*nlxi*idyxxgxfxtsxwsiixg*w*ixsnxx*x*xxfhpir
xcxlxhxhlhtnynqpxsitiimvpxx*sxxlskxtxrxqfxksvlsgspinssnavsxs
tl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK258 (VHK258Q) /CSM/VH/VHK2-C/VHK258Q.Seq.d/                        525   e-148
VHD183 (VHD183Q) /CSM/VH/VHD1-D/VHD183Q.Seq.d/                        444   e-124
CHG312 (CHG312Q) /CSM/CH/CHG3-A/CHG312Q.Seq.d/                        438   e-122
VHA108 (VHA108Q) /CSM/VH/VHA1-A/VHA108Q.Seq.d/                        436   e-121
SHJ811 (SHJ811Q) /CSM/SH/SHJ8-A/SHJ811Q.Seq.d/                        436   e-121
SHJ667 (SHJ667Q) /CSM/SH/SHJ6-C/SHJ667Q.Seq.d/                        436   e-121
SHG203 (SHG203Q) /CSM/SH/SHG2-A/SHG203Q.Seq.d/                        436   e-121
SHC731 (SHC731Q) /CSM/SH/SHC7-B/SHC731Q.Seq.d/                        436   e-121
CHS485 (CHS485Q) /CSM/CH/CHS4-D/CHS485Q.Seq.d/                        436   e-121
CHS185 (CHS185Q) /CSM/CH/CHS1-D/CHS185Q.Seq.d/                        436   e-121
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BX901937|BX901937.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-9I23.    42   5.4    1
dna update 2004. 3.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

L25926_1(L25926|pid:none) Rat sucrase-isomaltase (SI) mRNA, comp...    39   0.057
(P23739) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    39   0.057
(O62653) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    39   0.074
(Q9C0Y4) RecName: Full=Alpha-glucosidase;          EC=3.2.1.20; ...    38   0.13
EU937530_1(EU937530|pid:none) Mus musculus sucrase-isomaltase mR...    38   0.13
AB045751_1(AB045751|pid:none) Schizosaccharomyces pombe agl mRNA...    38   0.13
AB428422_1(AB428422|pid:none) Felis catus SI mRNA for sucrase-is...    38   0.13
(P70699) RecName: Full=Lysosomal alpha-glucosidase;          EC=...    37   0.28
BC115034_1(BC115034|pid:none) Homo sapiens sucrase-isomaltase (a...    37   0.28
(P14410) RecName: Full=Sucrase-isomaltase, intestinal; Contains:...    37   0.28
protein update 2009. 7.15
PSORT 

 psg: 0.86  gvh: 0.44  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.44  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 28.0 %: nuclear
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHK258 is mit
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHK258Z
3' end seq. 
>VHK258Z.Seq
NNNNNNNNNNATANGTTACACCAGTTGATGGTATTNCAATGACCACTAAAATCTCTAGAA
CCTTNCCATTTGAATTGATTATNGCCNTGGATTNTTCATNACAAGCAANTGGTCAATTAT
TNNTGGATGATGGTGAATCNATTCAAACNTATGNTGATNATAAANANTNTTTCATCCAAT
TCGATGNTGTNTCCTCANCCATNTTCATTTGCATACAAATTACAATCAACCANTCTCAAT
AACAATTATAATGGTACCGNTTNTTTAATCATNAANTCTATCCAAATANACGGNTCGCCA
NCAGTTCANCAAGTCAGTGTTAAGTGGTAGCCCAATCAATTCATCTAATGCTGTTTCNGA
NTCAACTCTCT
Length of 3' end seq. 371
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -