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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK248
Library VH
(Link to library)
Clone ID VHK248
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHK248P
(Link to Original site)
Representative DNA sequence 
>VHK248 (VHK248Q) /CSM/VH/VHK2-B/VHK248Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAXXXXXXXXXXCCAGAAG
GTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTA
AAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAA
ACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACA
GAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTNTATGCTGCTGAATTAACTG
CCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTNTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAANTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAAT
TTTAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLXSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRXRKGLAPEIPDLDKFHEKL*tx*val*vfknsnqi*kkf*k
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLXSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRXRKGLAPEIPDLDKFHEKL*tx*val*vfknsnqi*kkf*k

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflir---

---qkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvvyxmlln*lppqlx*nqxt**kllpqktllvvftlysivvxvll
lvknvvsvphyxlskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkpl
ksl*ppexekv*pqksqiltnstknskqxkshfefskivikskknfkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---rr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph
*chpqrwwsnhpncssctxcc*inclpnxxrtnxlsrnycprkrywwyllctqssxrycy
w*rtsyrfpiixcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*
srfshqkxkrfsprnprs*qiprktlnxlsrtlsfqk**snlkkilk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1219   0.0
VHQ470 (VHQ470Q) /CSM/VH/VHQ4-C/VHQ470Q.Seq.d/                       1219   0.0
VHQ395 (VHQ395Q) /CSM/VH/VHQ3-D/VHQ395Q.Seq.d/                       1219   0.0
VHP832 (VHP832Q) /CSM/VH/VHP8-B/VHP832Q.Seq.d/                       1219   0.0
VHP776 (VHP776Q) /CSM/VH/VHP7-D/VHP776Q.Seq.d/                       1219   0.0
VHP765 (VHP765Q) /CSM/VH/VHP7-C/VHP765Q.Seq.d/                       1219   0.0
VHP749 (VHP749Q) /CSM/VH/VHP7-C/VHP749Q.Seq.d/                       1219   0.0
VHP672 (VHP672Q) /CSM/VH/VHP6-C/VHP672Q.Seq.d/                       1219   0.0
VHP589 (VHP589Q) /CSM/VH/VHP5-D/VHP589Q.Seq.d/                       1219   0.0
VHP487 (VHP487Q) /CSM/VH/VHP4-D/VHP487Q.Seq.d/                       1219   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446440) Dictyostelium discoideum cDNA clone:ddv62a23, 3' ...  1257   0.0    1
(BJ446369) Dictyostelium discoideum cDNA clone:ddv62b17, 3' ...  1257   0.0    1
(BJ446358) Dictyostelium discoideum cDNA clone:ddv62p08, 3' ...  1257   0.0    1
(BJ445933) Dictyostelium discoideum cDNA clone:ddv61i04, 3' ...  1257   0.0    1
(BJ445765) Dictyostelium discoideum cDNA clone:ddv60g14, 3' ...  1257   0.0    1
(BJ443409) Dictyostelium discoideum cDNA clone:ddv52g22, 3' ...  1257   0.0    1
(BJ442954) Dictyostelium discoideum cDNA clone:ddv51o12, 3' ...  1257   0.0    1
(BJ442845) Dictyostelium discoideum cDNA clone:ddv51k04, 3' ...  1257   0.0    1
(BJ442655) Dictyostelium discoideum cDNA clone:ddv50g14, 3' ...  1257   0.0    1
(BJ442110) Dictyostelium discoideum cDNA clone:ddv48j21, 3' ...  1257   0.0    1
dna update 2008.11.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   258   2e-67
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   258   3e-67
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   256   1e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   254   3e-66
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   254   3e-66
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   254   4e-66
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   254   4e-66
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   253   7e-66
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   253   1e-65
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   252   2e-65
protein update 2009. 7.15
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHK248 is nuc
5' end seq. ID VHK248F
5' end seq. 
>VHK248F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGANNNNNNNNNN
Length of 5' end seq. 533
3' end seq. ID VHK248Z
3' end seq. 
>VHK248Z.Seq
NNNNNNNNNNCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCA
ATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGG
TGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCA
CACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTNTA
TGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTAC
TGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGT
TATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTNTCTGTCAAAGCTCATTTACCAGT
ATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCC
ACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCAC
TGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGA
CAAATTCCACGAAAAACTCTAAACAANTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAA
TCAAATCTAAAAAAAATTTTAAAAAA
Length of 3' end seq. 686
Connected seq. ID VHK248P
Connected seq. 
>VHK248P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGA----------CCAGAAG
GTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTA
AAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAA
ACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACA
GAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTNTATGCTGCTGAATTAACTG
CCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTNTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAANTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAAT
TTTAAAAAA
Length of connected seq. 1199
Full length Seq ID -
Full length Seq. -
Length of full length seq. -