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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK246
Library VH
(Link to library)
Clone ID VHK246
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16440-1
Original site URL
Representative seq. ID VHK246P
(Link to Original site)
Representative DNA sequence 
>VHK246 (VHK246Q) /CSM/VH/VHK2-B/VHK246Q.Seq.d/
AATATTATTTTTCTTCACTATCTGTTTATATAAACAAATTGGAGGAAATTATAAAATGAA
GATTTCATCTATTTTATTTATTTTTATAGCGATTATTGTTGGCTTAGCACTTTCACAAAA
TACTATTCCAACTTATAAGCAAAACAGCATTAATTGGAAGCAATTTCAACCATTATATAC
AAATAAAGATGATTATGTATTAATTGTAGATACAAGATCAAAGAATGTTTTAAAAAGATC
AACAAATGGTGGTATTGATTGGTCATCAAATGTACTTGATAAACAAGGAAATATTATTTA
TGGTAATGCAGGATATTATCAAAGAGATGATAATATTATGGTTGTAAATTTCCCATCTTA
TGGAAAAACCCAAATTTCAGTATCAAATGATGCAGGTCAAACATTTTCAACCTCAGCAGT
TTTTCCACATAAAGTTGTATCAGTTTCACCACATTTAGATATTGAGGGTTTAGTGATTGC
ACGTTCCGATAATTTCATTTTAATTAGTGAAGATTTTGGTTTAACATGGAACAATGCATC
ATCCAATGGTGATGTTTTATTCACACCAGGTGGTTCAAGCTTTCCAACCGACCCATTATG
GGATCCAXXXXXXXXXXCAAATGTTGATGTCATCAATATCATTACAAGTCCAAGAAATGA
TGGAAATAAATTCATCTTTTTAGTAGAGGATGAATATGATATCGGTCAAATGATTGGTGT
TGATTTAACAAATGTATTGCCATCTCCTTGCGATGATTCCTCAATGCAATCTTATACCAC
TCGTTGTATTTTAGGTGAAAAAACAACTTATAGAACTGTTAAACCAAATACCAATTGTTA
TATGACCCAACAAGCACAAATTTCAAATAAATATTGTGAATGTACAGTCGAAGATTTTGA
ATGTGATATTGGTTACGAAAGTGCAGACGATAGCAGTTCCGATAATGATGGTTCTACCTC
TGGTATTCCATCTGGATCTTCAGAACAATTGAATTGTCGTAAATCAATTGCTTCACAATA
TGTTCCAATCACTGAAGAAAATTGTACACCAGGTCAAACCTATATCAAATCAAAAGGTTT
CAGAAGAGTCGCTGCCACACAATGTGTAGGTGGTGTTAATGCAACTTATTCACCAGAGAT
TGTCATGTGTCCATCAAAAAATTCTTCAAGTGGTGGCAAAGGTTGGATCGCTGGTGTTGT
TATTGCTATTCTTGCTTTAGCTGGTCTTGGTGGTTTTGGTTTCTACGTTTTTAAGAATCC
AGATTTCAAACAAAAATTATTAAAAATGGTTGGTTTTTCAAAGGTCGGTCCAAAATATAG
TGTTATCGGTATTAAACCAAACTCATTAGCTGATGAGAATTTGGTAT
sequence update 2002.10.25
Translated Amino Acid sequence 
ILFFFTICLYKQIGGNYKMKISSILFIFIAIIVGLALSQNTIPTYKQNSINWKQFQPLYT
NKDDYVLIVDTRSKNVLKRSTNGGIDWSSNVLDKQGNIIYGNAGYYQRDDNIMVVNFPSY
GKTQISVSNDAGQTFSTSAVFPHKVVSVSPHLDIEGLVIARSDNFILISEDFGLTWNNAS
SNGDVLFTPGGSSFPTDPLWDP---

---NVDVINIITSPRNDGNKFIFLVEDEYDIGQMIGVDLTNVLPSPCDDSSMQSYTTRCI
LGEKTTYRTVKPNTNCYMTQQAQISNKYCECTVEDFECDIGYESADDSSSDNDGSTSGIP
SGSSEQLNCRKSIASQYVPITEENCTPGQTYIKSKGFRRVAATQCVGGVNATYSPEIVMC
PSKNSSSGGKGWIAGVVIAILALAGLGGFGFYVFKNPDFKQKLLKMVGFSKVGPKYSVIG
IKPNSLADENLV
Translated Amino Acid sequence (All Frames) 
Frame A:
niiflhylfi*tnwrkl*nedfiyfiyfysdycwlstftkyysnl*akqh*leaistiiy
k*r*lcincrykikecfkkinkwwy*lvikct**trkyylw*crilskr**yygckfpil
wknpnfsik*crsnifnlssfst*scisfttfry*gfsdctfr*fhfn**rfwfnmeqci
iqw*cfihtrwfklsnrpimgs---

---qmlmssislqvqemmeinssf**rmnmisvk*lvli*qmychllamipqcnliplvv
f*vkkqliellnqipivi*pnkhkfqinivnvqskilnvilvtkvqtiavpimmvlplvf
hldlqnn*ivvnqllhnmfqslkkivhqvkpisnqkvseeslphnv*vvlmqlihqrlsc
vhqkilqvvakvgslvllllfll*lvlvvlvstflriqisnkny*kwlvfqrsvqnivls
vlnqth*lmriwy

Frame B:
ILFFFTICLYKQIGGNYKMKISSILFIFIAIIVGLALSQNTIPTYKQNSINWKQFQPLYT
NKDDYVLIVDTRSKNVLKRSTNGGIDWSSNVLDKQGNIIYGNAGYYQRDDNIMVVNFPSY
GKTQISVSNDAGQTFSTSAVFPHKVVSVSPHLDIEGLVIARSDNFILISEDFGLTWNNAS
SNGDVLFTPGGSSFPTDPLWDP---

---kc*chqyhykskk*wk*ihlfsrg*i*yrsndwc*fnkciaislr*flnailyhsly
fr*knnl*nc*tkyqllydptstnfk*il*mysrrf*m*ywlrkcrr*qfr**wfylwys
iwifrtiels*incfticsnh*rklytrsnlyqikrfqksrchtmcrwc*cnlftrdchv
sikkffkwwqrldrwccycyscfswswwfwflrf*esrfqtkiikngwffkgrski*cyr
y*tklis**efg

Frame C:
yyfsslsvyinkleeiik*rfhlfylfl*rllla*hfhkilfqlisktaligsnfnhyiq
ikmimy*l*iqdqrmf*kdqqmvvlighqmylinkeilfmvmqdiikemiilwl*ishlm
ekpkfqyqmmqvkhfqpqqffhiklyqfhhi*ilrv**lhvpiisf*lvkilv*hgtmhh
pmvmfyshqvvqafqpthygi---

---NVDVINIITSPRNDGNKFIFLVEDEYDIGQMIGVDLTNVLPSPCDDSSMQSYTTRCI
LGEKTTYRTVKPNTNCYMTQQAQISNKYCECTVEDFECDIGYESADDSSSDNDGSTSGIP
SGSSEQLNCRKSIASQYVPITEENCTPGQTYIKSKGFRRVAATQCVGGVNATYSPEIVMC
PSKNSSSGGKGWIAGVVIAILALAGLGGFGFYVFKNPDFKQKLLKMVGFSKVGPKYSVIG
IKPNSLADENLV
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK246 (VHK246Q) /CSM/VH/VHK2-B/VHK246Q.Seq.d/                       2650   0.0
VHP381 (VHP381Q) /CSM/VH/VHP3-D/VHP381Q.Seq.d/                       1473   0.0
SLB162 (SLB162Q) /CSM/SL/SLB1-C/SLB162Q.Seq.d/                       1473   0.0
AHL194 (AHL194Q) /CSM/AH/AHL1-D/AHL194Q.Seq.d/                       1473   0.0
VHK292 (VHK292Q) /CSM/VH/VHK2-D/VHK292Q.Seq.d/                       1467   0.0
VHB464 (VHB464Q) /CSM/VH/VHB4-C/VHB464Q.Seq.d/                       1467   0.0
VHA856 (VHA856Q) /CSM/VH/VHA8-C/VHA856Q.Seq.d/                       1465   0.0
VHA250 (VHA250Q) /CSM/VH/VHA2-C/VHA250Q.Seq.d/                       1465   0.0
VHQ426 (VHQ426Q) /CSM/VH/VHQ4-B/VHQ426Q.Seq.d/                       1461   0.0
VHB825 (VHB825Q) /CSM/VH/VHB8-B/VHB825Q.Seq.d/                       1461   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442936) Dictyostelium discoideum cDNA clone:ddv51k12, 3' ...  1487   0.0    1
(BJ436550) Dictyostelium discoideum cDNA clone:ddv31c14, 3' ...  1465   0.0    1
(BJ354523) Dictyostelium discoideum cDNA clone:dda53k23, 3' ...  1465   0.0    1
(BJ443077) Dictyostelium discoideum cDNA clone:ddv51g24, 3' ...  1459   0.0    1
(BJ437266) Dictyostelium discoideum cDNA clone:ddv33p16, 3' ...  1459   0.0    1
(BJ436944) Dictyostelium discoideum cDNA clone:ddv32p14, 3' ...  1457   0.0    1
(BJ446129) Dictyostelium discoideum cDNA clone:ddv61b21, 3' ...  1455   0.0    2
(BJ446610) Dictyostelium discoideum cDNA clone:ddv63d08, 3' ...  1453   0.0    1
(BJ437433) Dictyostelium discoideum cDNA clone:ddv34b08, 3' ...  1453   0.0    1
(BJ380467) Dictyostelium discoideum cDNA clone:ddc38c08, 3' ...  1453   0.0    1
dna update 2008.11.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF159157_1(AF159157|pid:none) Hydra vulgaris head-activator bind...    77   1e-12
AB093294_1(AB093294|pid:none) Mus musculus mRNA for mKIAA1329 pr...    76   3e-12
(Q9EPR5) RecName: Full=VPS10 domain-containing receptor SorCS2; ...    76   3e-12
BC156053_1(BC156053|pid:none) Synthetic construct Homo sapiens c...    75   7e-12
AB037750_1(AB037750|pid:none) Homo sapiens mRNA for KIAA1329 pro...    74   1e-11
(Q95209) RecName: Full=Sortilin-related receptor; AltName: Full=...    70   2e-10
AF284755_1(AF284755|pid:none) Mus musculus VPS10 domain receptor...    70   2e-10
(Q9JLC4) RecName: Full=VPS10 domain-containing receptor SorCS1; ...    70   2e-10
AY099453_1(AY099453|pid:none) Homo sapiens VPS10 domain receptor...    67   1e-09
AB209142_1(AB209142|pid:none) Homo sapiens mRNA for VPS10 domain...    67   1e-09
protein update 2009. 7.15
PSORT 

 psg: 0.65  gvh: 0.69  alm: 0.15  top: 0.47  tms: 0.13  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 28.0 %: endoplasmic reticulum
	 16.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: vacuolar
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHK246 is mit
5' end seq. ID VHK246F
5' end seq. 
>VHK246F.Seq
AATATTATTTTTCTTCACTATCTGTTTATATAAACAAATTGGAGGAAATTATAAAATGAA
GATTTCATCTATTTTATTTATTTTTATAGCGATTATTGTTGGCTTAGCACTTTCACAAAA
TACTATTCCAACTTATAAGCAAAACAGCATTAATTGGAAGCAATTTCAACCATTATATAC
AAATAAAGATGATTATGTATTAATTGTAGATACAAGATCAAAGAATGTTTTAAAAAGATC
AACAAATGGTGGTATTGATTGGTCATCAAATGTACTTGATAAACAAGGAAATATTATTTA
TGGTAATGCAGGATATTATCAAAGAGATGATAATATTATGGTTGTAAATTTCCCATCTTA
TGGAAAAACCCAAATTTCAGTATCAAATGATGCAGGTCAAACATTTTCAACCTCAGCAGT
TTTTCCACATAAAGTTGTATCAGTTTCACCACATTTAGATATTGAGGGTTTAGTGATTGC
ACGTTCCGATAATTTCATTTTAATTAGTGAAGATTTTGGTTTAACATGGAACAATGCATC
ATCCAATGGTGATGTTTTATTCACACCAGGTGGTTCAAGCTTTCCAACCGACCCATTATG
GGATCCANNNNNNNNNN
Length of 5' end seq. 617
3' end seq. ID VHK246Z
3' end seq. 
>VHK246Z.Seq
NNNNNNNNNNCAAATGTTGATGTCATCAATATCATTACAAGTCCAAGAAATGATGGAAAT
AAATTCATCTTTTTAGTAGAGGATGAATATGATATCGGTCAAATGATTGGTGTTGATTTA
ACAAATGTATTGCCATCTCCTTGCGATGATTCCTCAATGCAATCTTATACCACTCGTTGT
ATTTTAGGTGAAAAAACAACTTATAGAACTGTTAAACCAAATACCAATTGTTATATGACC
CAACAAGCACAAATTTCAAATAAATATTGTGAATGTACAGTCGAAGATTTTGAATGTGAT
ATTGGTTACGAAAGTGCAGACGATAGCAGTTCCGATAATGATGGTTCTACCTCTGGTATT
CCATCTGGATCTTCAGAACAATTGAATTGTCGTAAATCAATTGCTTCACAATATGTTCCA
ATCACTGAAGAAAATTGTACACCAGGTCAAACCTATATCAAATCAAAAGGTTTCAGAAGA
GTCGCTGCCACACAATGTGTAGGTGGTGTTAATGCAACTTATTCACCAGAGATTGTCATG
TGTCCATCAAAAAATTCTTCAAGTGGTGGCAAAGGTTGGATCGCTGGTGTTGTTATTGCT
ATTCTTGCTTTAGCTGGTCTTGGTGGTTTTGGTTTCTACGTTTTTAAGAATCCAGATTTC
AAACAAAAATTATTAAAAATGGTTGGTTTTTCAAAGGTCGGTCCAAAATATAGTGTTATC
GGTATTAAACCAAACTCATTAGCTGATGAGAATTTGGTAT
Length of 3' end seq. 760
Connected seq. ID VHK246P
Connected seq. 
>VHK246P.Seq
AATATTATTTTTCTTCACTATCTGTTTATATAAACAAATTGGAGGAAATTATAAAATGAA
GATTTCATCTATTTTATTTATTTTTATAGCGATTATTGTTGGCTTAGCACTTTCACAAAA
TACTATTCCAACTTATAAGCAAAACAGCATTAATTGGAAGCAATTTCAACCATTATATAC
AAATAAAGATGATTATGTATTAATTGTAGATACAAGATCAAAGAATGTTTTAAAAAGATC
AACAAATGGTGGTATTGATTGGTCATCAAATGTACTTGATAAACAAGGAAATATTATTTA
TGGTAATGCAGGATATTATCAAAGAGATGATAATATTATGGTTGTAAATTTCCCATCTTA
TGGAAAAACCCAAATTTCAGTATCAAATGATGCAGGTCAAACATTTTCAACCTCAGCAGT
TTTTCCACATAAAGTTGTATCAGTTTCACCACATTTAGATATTGAGGGTTTAGTGATTGC
ACGTTCCGATAATTTCATTTTAATTAGTGAAGATTTTGGTTTAACATGGAACAATGCATC
ATCCAATGGTGATGTTTTATTCACACCAGGTGGTTCAAGCTTTCCAACCGACCCATTATG
GGATCCA----------CAAATGTTGATGTCATCAATATCATTACAAGTCCAAGAAATGA
TGGAAATAAATTCATCTTTTTAGTAGAGGATGAATATGATATCGGTCAAATGATTGGTGT
TGATTTAACAAATGTATTGCCATCTCCTTGCGATGATTCCTCAATGCAATCTTATACCAC
TCGTTGTATTTTAGGTGAAAAAACAACTTATAGAACTGTTAAACCAAATACCAATTGTTA
TATGACCCAACAAGCACAAATTTCAAATAAATATTGTGAATGTACAGTCGAAGATTTTGA
ATGTGATATTGGTTACGAAAGTGCAGACGATAGCAGTTCCGATAATGATGGTTCTACCTC
TGGTATTCCATCTGGATCTTCAGAACAATTGAATTGTCGTAAATCAATTGCTTCACAATA
TGTTCCAATCACTGAAGAAAATTGTACACCAGGTCAAACCTATATCAAATCAAAAGGTTT
CAGAAGAGTCGCTGCCACACAATGTGTAGGTGGTGTTAATGCAACTTATTCACCAGAGAT
TGTCATGTGTCCATCAAAAAATTCTTCAAGTGGTGGCAAAGGTTGGATCGCTGGTGTTGT
TATTGCTATTCTTGCTTTAGCTGGTCTTGGTGGTTTTGGTTTCTACGTTTTTAAGAATCC
AGATTTCAAACAAAAATTATTAAAAATGGTTGGTTTTTCAAAGGTCGGTCCAAAATATAG
TGTTATCGGTATTAAACCAAACTCATTAGCTGATGAGAATTTGGTAT
Length of connected seq. 1357
Full length Seq ID -
Full length Seq. -
Length of full length seq. -